• Journal of Korean Society of Steel Construction
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• v.29 no.1
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• pp.81-88
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• 2017

Residual stress is defined as stress that already exists on a structural member from the effects of welding and plastic deformation before the application of loading. Due to such residual stress, welded H-section compression members under centroidal compression load can undergo buckling and failure for strength values smaller than the predicted buckling load and specified compressive strength. Therefore, this study was carried out to evaluate the effect of residual stress from welding on the determination of the buckling load and specified compressive strength of the H-section compression member according to the column length variation. A three-dimensional nonlinear finite element analysis was performed for the H-section compression member where the welded joint was fillet welded by applying heat inputs of 3.1kJ/mm and 3.6kJ/mm using the SAW welding method.

• Journal of the Korean Geotechnical Society
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• v.29 no.6
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• pp.53-62
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• 2013

A 5-20 cm thick shocrete has been routinely constructed for NATM tunneling method to stabilize and confine the excavated rock of tunnel construction site. A $40kg/m^3$ of steel fibers are usually mixed into such shotcrete but these steel fibers may not be evenly distributed depending on shotcrete machines, mixing ratios and excavated rock conditions. In this study, square column shotcrete-mimicked concrete specimens of $15cm{\times}15cm{\times}55cm$ were prepared with 5 equal layers and 5 or 20% cement ratio. The specimens were prepared with different reinforced-patterns: non-reinforced, middle layer-reinforced, 1, 3, and 5 layers-reinforced, or all layers reinforced. The specimens were air-cured for 7 days and tested for flexural strength. The influence of steel-fiber distribution on flexural strength and toughness of shotcrete-mimicked concrete specimens was investigated. In the case of a specimen with cement ratio of 20%, a flexural strength increased as a number of fiber-reinforced layer increased. The flexural strength of one-layer reinforced specimen showed 20% less than that of evenly fiber-distributed specimen. On the other hand, a specimen with cement ratio of 5% decreased as the number of fiber-reinforced layers increased. A toughness index increased as the number of fiber-reinforced layers increased, regardless of cement ratios. The toughness index of evenly fiber-distributed specimen showed 2-3 times as large as that of one-layer reinforced specimen.

• Journal of the Korea Concrete Institute
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• v.21 no.1
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• pp.105-116
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• 2009

Reinforced concrete (RC) structures have been most widely used because of their good economic efficiency. However, it is very weak in tensile stresses and difficult to control deflection due to the heavy self-weight of concrete. On the other hand, it is generally known that prestressed concrete structures can be the most effective to overcome the demerits of RC structures by using various tendon lay-out and its amount. In the prestressed concrete members, the inflection points of tendons should be placed effectively for the deflection control and the moment reduction. Therefore, in this study, the equations of tendon profiles are derived in terms of polynomials that satisfy essential conditions of tendon geometries such as inflection points and natural curved shapes of tendons placed in continuous members, from which vertical components of prestressing forces can be also calculated. The derived high order polynomial expression for the distributed shape of the upward and downward forces was transformed to an simplified equivalent uniform vertical force in order to improve the applicability in the calculation of member deflection. The influences of vertical forces by tendons to deflection and moment in a continuous slab were also considered depending on the distance from column face to the location of tendons. The applicability of the proposed method was examined by an example of deflection calculation for the cases of slabs with and without tendons, and the efficiency of deflection control by tendons was also quantitatively estimated.

• Journal of Korean Society of Forest Science
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• v.99 no.6
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• pp.885-890
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• 2010

This study was conducted to confirm the application as ingredients of cosmetics through an examination of the function for anti-oxidant activity of the fraction isolated from Pyrus ussuriensis leaves. The dried leaf of Pyrus ussuriensis were extracted with acetone-$H_2O$ (6:4, v/v), concentrated and fractionated with the upper layer of acetone on a separatory funnel. Each fraction was freeze dried, then a portion of acetone soluble powder was chromatographed on a Sephadex LH-20 column using a series of aqueous methanol as eluents and also used the MIC-gel using a series of aqueous methanol as developing solvent. The isolated compounds were identified by silica-gel TLC. The concentration of total phenolic compound of Pyrus ussuriensis acetate soluble fraction was high, 914 mg/g. The results obtained from the analyses of the anti-oxidanat effects of Pyrus ussuriensis acetate fraction can be summarized as follows: In the result of DPPH scavenging radical activity, Pyrus ussuriensis acetate soluble fraction showed more than 80% at 100 ppm. SOD-like activity of one of Pyrus ussuriensis acetate soluble fractions was 77% at 1000 ppm. Xanthine oxidase inhibition of Pyrus ussuriensis acetate soluble fraction was 38% at 100 ppm. From these results, we confirmed that acetate fraction of Pyrus ussuriensis has a great potential as a natural ingredients with a natural antioxidant and antimicrobial source.

• Biomolecules & Therapeutics
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• v.22 no.4
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• pp.347-354
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• 2014

Larrea nitida is a plant that belongs to the Zygophyllaceae family and is widely used in South America to treat inflammatory diseases, tumors and menstrual pain. However, its pharmacological activity remains unclear. In this study we evaluated the property of selective estrogen receptor modulator (SERM) of Larrea nitida extracts (LNE) as a phytoestrogen that can mimic, modulate or disrupt the actions of endogenous estrogens, depending on the tissue and relative amount of other SERMs. To investigate the property of SERM of LNE, we performed MCF-7 cell proliferation assays, estrogen response element (ERE)-luciferase reporter gene assay, human estrogen receptor (hER) binding assays and in vivo uterotrophic assay. To gain insight into the active principles, we performed a bioassay-guided analysis of LNE employing solvents of various polarities and using classical column chromatography, which yielded 16 fractions (LNs). LNE showed high binding affinities for $hER{\alpha}$ and $hER{\beta}$ with $IC_{50}$ values of $1.20{\times}10^{-7}$ g/ml and $1.00{\times}10^{-7}$ g/ml, respectively. LNE induced $17{\beta}$-estradiol (E2)-induced MCF-7 cell proliferation, however, it reduced the proliferation in the presence of E2. Furthermore, LNE had an atrophic effect in the uterus of immature rats through reducing the expression level of progesterone receptor (PR) proteins. LN08 and LN10 had more potent affinities for binding on $hER{\alpha}$ and ${\beta}$ than other fractions. Our results indicate that LNE had higher binding affinities for $hER{\beta}$ than $hER{\alpha}$, and showed SERM properties in MCF-7 breast cancer cells and the rat uterus. LNE may be useful for the treatment of estrogen-related conditions, such as female cancers and menopause.

• Journal of the Earthquake Engineering Society of Korea
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• v.6 no.3
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• pp.23-29
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• 2002

A wall-frame type structural system has been widely used to make full use of a limited land in large cities to satisfy the several functional requirement in one building. However, this type of hybrid structure brought some problems due to the vertical discontinuity of a structural system. The response of a wall-frame type structural system having a deep transfer girder was observed. An arch system was introduced to replace the deep transfer girder. The adequacy of an arch system was observed for the various boundary conditions of a system. The proposed system was compared to a general transfer girder system by applying both gravity load and lateral load. It was observed that an arch system fairly distributes the stress without concentrating stress at a certain location of a system differently from the current transfer girder system. The moment decrement effect of a column can also be obtained by eliminating the large mass of a transfer girder. Also it was investigated that an arch system is more economical and effective than the current transfer girder system.

• Journal of the korean society of oceanography
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• v.33 no.4
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• pp.168-177
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• 1998

Five and a half months after the Keumdong oil spill accident on the 21$^{st}$ of September 1993, 34 seawater samples and 94 sediment samples were collected from Kwangyang Bay and Namhaedo area to assess its environmental impacts. Hydrocarbon concentration in the seawater ranged from 0.8 to 9.2 ${\mu}$g/1 with an average of 3.3 ${\mu}$g/1. This average value was nearly the same as the value(3.7 ${\mu}$g/1) before the oil spill accident. This suggests that by the early March of 1994 majority of the coastal water in the study area restored to its background hydrocarbon concentration before the oil spill accident. Nutrients, heavy metals and other general environmental parameters of the seawater did not show any aggravated seawater quality compared with the previous records. From the regression analysis of time-course observation of hydrocarbon in the seawater, except the sediment environment, the effect of oil spill on the water column was estimated to last at least 4 months in the study area after the oil spill accident. In the shoreline sediments, oil deposits were, however, still found at the high water marks at several stations, and very high values were found in the west of Namhaedo, ranging from 3.7 to 40.1 mg/g of wet sediment. Gas chromatography of these samples showed a very distinct Bunker C chromatogram identical to the Keumdong oil spill. Hydrocarbons in the subtidal bottom sediments in the study area and the reference stations (YB and CB) ranged from 0.45 to 18.08 ${\mu}$g/g of wet sediment with an average of 3.09 ${\mu}$g/g. West of Namhaedo (Stations Bl2-B33) generally showed much higher values than inner Kwangyang Bay and in Chinju Bay. Chinju Bay generally showed the lowest value among the study area. Subtidal bottom sediments in inner Kwangyang Bay and Chinju Bay seemed to be less affected than west of Namhaedo. Heavy metal concentrations in the sediment were relatively higher in the Kwangyang Bay than in the Chinju Bay. However, metal concentrations in the study area were in general comparable to the reference areas.

• Journal of Korean Society of Steel Construction
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• v.22 no.2
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• pp.151-160
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• 2010

The number of cases of collapsed plastic greenhouses in farmlands has increased due to the heavy local snowfall caused by extraordinary atmospheric phenomena. Consequently, the economic losses of farmers have also increased. However the government policy in relation to damage pretension is insufficient and collapse case is repeated every year. The main reason for frame collapse is that the moment capacity of a steel pipe is not sufficient to resist a heavy snowload. In this study, experiments were conducted on the current frame system of a greenhouse with a tension tie. The frame consisted of two sections(${\phi}25.4{\times}1.5$, ${\phi}31.8{\times}1.5$), and its span length was 6.5 m. A temporary tension tie using a steel wire and a fabric rope was connected to the two joints, to which a curved beam and a straight column were connected. The pretension force was applied at the tension tie, and a vertical force simulating snowfall was applied until failure. The fabric rope frame increased the load-carrying capacity by 10-45% compared to the normal frame without a tension tie, and the steel wire frame increased the load-carrying capacity by 58-73% compared to the normal frame without a tension tie. Steel wire was found to be more effective as far as strength is concerned, but its connection details and pretension application are more difficult and complicated than those of the fabric rope. The test results thus show that the fabric rope is more preferable.

• Journal of Soil and Groundwater Environment
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• v.14 no.6
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• pp.101-107
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• 2009

Carboxymethyl cellulose (CMC) as stabilizer is expected to facilitate in-situ delivery of zero-valent iron (ZVI) nanoparticles in a contaminated aquifer because it increases dispersity of ZVI nanoparticles. This work investigated the transport of CMC-stabilized ZVI nanoparticles (CMC-Fe) using column breakthrough experiments. The ZVI nanoparticles (100 mg/L Fe) were transportable through sand porous media. In contrast, non-stabilized ZVI nanoparticles rapidly agglomerate in solution and are stopped in sand porous media. At pH 7 of solution approximately 80% CMC-Fe were eluted. When the pH of solution is below 5, 100% CMC-Fe were eluted. These results suggest that the mobility of CMCFe was increased as pH decreases. In the mobility test under different ionic strengths using $Na^+$ and $Ca^{2+}$ ions, there was no signigficant difference in the mobility of CMC-Fe. Also, in the experiments of effect of clay and natural organic mater (NOM) on the mobility of ZVI, there was no significant difference in the mobility of CMC-Fe not only between 1 and 5% clay, but 100 and 1000 mg/L NOM. The results from this work suggests that the CMC-Fe nanoparticles could be easily delivered into the subsurface over a broad range of ionic strength, clay and NOM.

• Microbiology and Biotechnology Letters
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• v.32 no.2
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• pp.142-148
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• 2004

Erythropoietin is a main regulator of human erythropoiesis. Recombinant human erythropoietin (rhEPO) is one of the glycoproteins produced in animal cells, and it has oligo saccharides chains which comprise about 40％ of its molecular mass. Because the content of sialic acid can extend circulatory lifetime, the high degree of sialylation is often a desirable feature of therapeutic glycoproteins. In this study, the sialylation of rhEPO produced by chinese hamster ovary cell culture was maximized by supplementing the culture medium with N-acetylm-annosamine (ManNAc), a direct intracellular precursor for sialic acid synthesis and 2-deoxy-2,3-dehydro-N-acetylneuraminic acid (NeuAc2en), a sialidase inhibitor. Feeding of 20 mM ManNAc/0.5 mM NeuAc2en into culture medium increased the sialic acid content by nearly tenfold compared with unsupplemented medium. This effect was achieved without affecting the cell growth or product yield. Six erythropoietin fractions differing in sialic acid content, ranging from 11∼15％ of EPO, were identified from chinese hamster ovary cell-derived rhEPO by mono Q column chromatography. It was found that, at 20 mM ManNAc/0.5 mM NeuAc2en feeding, productivity of hyper-glycosylated EPO increased up to 50％, compared with the unsupplemented medium.