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Antioxidant Effect of the Fraction Isolated from Pyrus ussuriensis Leaves  

Lee, Chang-Eon (Department of Cosmeceutical Science, Daegu Haany University)
Kim, Young-Hun (Skin Science R&D Center, Mediway Korea Co., Ltd.)
Lee, Byung-Guen (Department of Forest Resources, Yeungnam University)
Lee, Do-Hyung (Department of Forest Resources, Yeungnam University)
Publication Information
Journal of Korean Society of Forest Science / v.99, no.6, 2010 , pp. 885-890 More about this Journal
Abstract
This study was conducted to confirm the application as ingredients of cosmetics through an examination of the function for anti-oxidant activity of the fraction isolated from Pyrus ussuriensis leaves. The dried leaf of Pyrus ussuriensis were extracted with acetone-$H_2O$ (6:4, v/v), concentrated and fractionated with the upper layer of acetone on a separatory funnel. Each fraction was freeze dried, then a portion of acetone soluble powder was chromatographed on a Sephadex LH-20 column using a series of aqueous methanol as eluents and also used the MIC-gel using a series of aqueous methanol as developing solvent. The isolated compounds were identified by silica-gel TLC. The concentration of total phenolic compound of Pyrus ussuriensis acetate soluble fraction was high, 914 mg/g. The results obtained from the analyses of the anti-oxidanat effects of Pyrus ussuriensis acetate fraction can be summarized as follows: In the result of DPPH scavenging radical activity, Pyrus ussuriensis acetate soluble fraction showed more than 80% at 100 ppm. SOD-like activity of one of Pyrus ussuriensis acetate soluble fractions was 77% at 1000 ppm. Xanthine oxidase inhibition of Pyrus ussuriensis acetate soluble fraction was 38% at 100 ppm. From these results, we confirmed that acetate fraction of Pyrus ussuriensis has a great potential as a natural ingredients with a natural antioxidant and antimicrobial source.
Keywords
Pyrus ussuriensis; Anti-inflammatory and anti-microbial; anti-oxidant; SOD;
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