• Applied Biological Chemistry
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• v.39 no.4
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• pp.249-254
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• 1996

The volatile flavor components in mash of nonglutinous rice Takju brewed with or without addition of the starter were determined by GC and GC-MS using polar column. Various kinds of volatile components including alcohols(10), esters(9), organic acids(2) and aldehydes(7) were found in mash of nonglutinous rice Takjus. At the beginning stage of fermentation, 3 to 7 volatile flavor components were detected. The number of volatile flavor components were increased to 26 of to 28 after 16 days fermentation. Early in the brewing, just 3 kinds of flavor components including ethyl acetate, ${\beta}-phenylethyl$ acetate and acetic acid were detected in mash of nonglutinous rice Takju without the addition of the starter, when starter is added additional compounds of alcohol including ethanol were detected, which is suggested to be the effect of flavors originated from the starter. The common flavor compounds through the entire process of fermentation in mash of nonglutinous rice Takju were ethyl acetate, ${\beta}-phenylethyl$ acetate and acetic acid. The relative amount(peak area %) of flavor components were changed with the fermentation period, but the major volatile flavor components were ethyl alcohol, iso-amyl alcohol, hexenyl alcohol, ethyl acetate, ${\beta}-phenylethyl$ acetate and ${\beta}-phenylethyl$ alcohol. The relative amount(peak area %) of ethyl alcohol was the highest in mash of nonglutinous rice Takju with addition of starter. When the starter was not added in the mash of nonglutinous rice Takju, any alcoholic components were not detected in the early stage of fermentation. But the percent of peak area hexenyl alcohol was the highest after 16 days fermentation. Especially few sorts of ester were detected in mash of nonglutious rice Takju without addition of starter by 3 days fermentation.

• Proceedings of the Turfgrass Society of Korea Conference
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• 2011.02a
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• pp.5-8
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• 2011

This research was conducted to determine the effect of capillary rise interruption layer on the sand based growing media when growing Kentucky bluegrass under soil reclamation and saline water irrigation. Rootzone profile consists of three layers as top soil of 30 cm, 20 cm of capillary interruption layer and 10 cm of reclaimed paddy soil. Rootzone profile was packed in column pots. The top soil was a mixture of sand dredged up from Lake Bhunam Tae Ahn, Korea and peat at the ratio of 95:5 by volume. Bottom part of column was covered with plastic net and the pots were soaked into 5 cm depth saline water reservoir with salinity $3-5dsm^{-1}$. Kentucky bluegrass was installed by sod and irrigated using $2dSm^{-1}$ saline water(5.7mm $day^{-1}$)in 3days interval. The results showed that the largest accumulation of salt in the spring with ECe of $5.4dSm^{-1}$ and SAR34.0 in rootzone with out capillary rise interruption layer and ECe of $4.6dSm^{-1}$ and SAR8.24 at rootzone using gravel as capillary rise interruption layer material. Kentucky bluegrass grown in growing media with gravel as capillary rise interruption layer resulted in the average visual quality rate of 8.1and clipping dry weight of $24.8gm^{-2}$, while Kentucky bluegrass grown in the growing media with out capillary rise interruption layer showed the visual quality rate of 7.9 and clipping dry weight of $34g.m^{-2}$. Capillary rise interruption layer of gravel and coarses and enhanced the visual quality by 4.1and 4.0%, root length by 50 and 38%, and root dryweight by 35and 17% of Kentucky bluegrass, and reduced the accumulation of Na by 16% and 25%, ECe by 7% and 13% in the rootzone.

• Korean Journal of Food Science and Technology
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• v.29 no.6
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• pp.1248-1254
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• 1997

To measure antioxidative activities, the various extracts from RVS (Rhus Verniciflua Stokes) were tried out with either DPPH or thiocyanate method. Also we used the GO (Glucose Oxidase) 20 mU/mL hydroxyl radical system in mouse whole brain cell culture. Chloroform, n-hexane or ethanol were used as extract solutions which had different polarity respectively. In DPPH and thiocyanate method, the antioxidative activities of the crude ethanol extracts were stronger than other extracts. The crude ethanol extracts were fractionated 5 peaks by glass column. Among of them, antioxidative activity of peak II $(P_{II})$ was shown stronger than other fractions, a little for peak III $(P_{III})$ and peak IV $(P_{IV})$, and none for peak I $(P_I)$ and Peak V $(P_V)$. In the antioxidative effects of crude ethanol extracts (30 mg/mL), cell viabilities were evaluated $1\;{\mu}L\;(297\;{\mu}g/mL)$, $2\;{\mu}L\;(588\;{\mu}g/mL)$ of crude ethanol extracts 59%, 68% respectively. $10\;{\mu}L\;(2,727\;{\mu}g/mL)$ addition of crude ethanol extracts had 95% cell viabilities, 0.01% significant, comparing control. In addition, the compounds related to antioxidative effect of crude ethanol extract might be glycoproteins by means of SDS-PAGE. Comparison to antioxidative effects between several antioxidants (ascorbic acid, ${\alpha}-tocopherol$, catalase) $273\;{\mu}L/mL$ addition of crude ethanol extracts corresponds to $1\;{\mu}g/mL$ catalase in antioxidative effects.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.10
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• pp.1378-1387
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• 2012

To investigate the pharmacological activity of chaga mushroom (Inonotus obliquus) on extraction conditions, chaga was extracted using water (reflux at $50^{\circ}C$, decoction over $90^{\circ}C$, pressure at $121^{\circ}C$) or ethanol (reflux at 50, 70, or $90^{\circ}C$). When water extract was further fractionated into crude polysaccharide (IO-CP), yields of IO-CP (4.8~16.8%) were higher than those of ethanolic extracts (IO-E, 1.9~2.7%) at increased temperature. For antioxidant activity, crude polysaccharide (IO-CP-121) obtained by pressurized extraction showed the highest polyphenolic and flavonoid contents (35.10 mg TAE/g and 18.48 mg QE/g, respectively) as well as DPPH and ABTS free radical scavenging activities (26.08 and 27.99 mg AEAC/100 mg, respectively). Meanwhile, IO-CP-D (decoction) and IO-CP-50 (reflux) had more potent mitogenic effects (2.10- and 1.95-fold of saline control at 100 ${\mu}g/mL$) as well as intestinal immune system modulating activities (6.30- and 5.74-fold) compared to IO-CP-121, whereas ethanolic extracts showed no activity. Although no IO-CP showed cytotoxicity against RAW 264.7 cells at 0.1 mg/mL, IO-CP-121 significantly inhibited TNF-${\alpha}$ and NO production as pro-inflammatory factors in LPS-stimulated RAW 264.7 cells (29.2 and 63.5%, respectively). Ethanolic extracts also showed no cytotoxicity at 0.1 mg/mL, whereas inhibition of TNF-${\alpha}$ and NO production was significantly low compared to that of IO-CP-121. In addition, active IO-CP-D was further fractionated into an unadsorbed (IO-CP-I) and seven adsorbed fractions (IO-CP-II~VIII) by DEAE-Sepharose CL-6B column chromatography in order to isolate immunostimulating polysaccharide. IO-CP-II showed the most potent mitogenic effect and macrophage stimulating activity (4.51- and 1.64-fold, respectively). IO-CP-II mainly contained neutral sugars (61.86%) in addition to a small amount of uronic acid (2.96%), and component sugar analysis showed that IO-CP-II consisted mainly of Glc, Gal, and Man (molar ratio of 1.00:0.55:0.31). Therefore, extraction conditions affect the physiological activity of chaga, and immunostimulating polysaccharide fractionated from chaga by decoction is composed mainly of neutral sugars.

• Journal of Dairy Science and Biotechnology
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• v.14 no.2
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• pp.157-164
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• 1996

This study aimed increase the quality during ripening of Cheddar cheese made with proteinase-negative mutant of Streptococcus lactis KCTC 1913 selected by curing. The degradation of protein during cheese ripening were investigated by electrophoresis and chromatography. The results were summarized as follow ; 1. The number of lactic acid bacteria decreased with the ripening stage, and that of the control cheese decreased faster than that of the cheese made with mutant. 2. Polyacrylamide gel electrophoretic analysis of cheese caseins revealed no difference between the cheese made with mutant and the control cheese, but differences along with the ripening stage were evident. 3. On Sephadex G-25 column chromatography, the extracts of bitter components from the green cheese and 3 month ripended cheese were fractionated into 3 fractions. With the progress of ripening, bitter peptides were degraded to rather small peptides or free amino acids. 4. Sensory evaluation of the 3 month ripended Cheddar cheese found no significant differences in color but the cheese made with mutant evidenced higher palatability in flavor and better texture than the control cheese. 5. The yields of the cheddar cheese made with mutant was 0.14% higher than that of the control cheese.

• Journal of agriculture & life science
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• v.44 no.4
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• pp.45-55
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• 2010

In this study, the acetylcholinesterase (AChE) inhibition and neuronal cell protective effects of water, 100% methanol and dichlromethane extracts from garlic were investigated. We found that dichloromethane extract of garlic resulted in a dose-dependent manner on AChE inhibition ($IC_{50}$: $36.1{\mu}g/mL$). In cell viability assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide (MTT), cell viabilities of water, 100% methanol and dichlromethane extracts were lower (almost under 40%) than amyloid ${\beta}$ protein ($A{\beta}$)-induced neurotoxicity. Because $A{\beta}$ is also known to increase neuronal cell membrane breakdown, neuronal apoptosis was further confirmed by lactate dehydrogenase (LDH) and neutral red uptake (NRU) assay. Water extract presented relative protection against $A{\beta}$-induced membrane damage in LDH assay. However all garlic extracts showed significant problem with decrease of cell viability in NRU assay, especially at dichloromethan extract. To determine active compounds in column fractions (98:2 fraction) from dichloromethane extract which showed significant AChE inhibitory effect, we performed HPLC and LC-MS analysis. It was supposed that garlic may contain allyl methyl disulfide, diallyl monosulfide, and diallyl disulfide as active compounds.

• Korean Journal of Remote Sensing
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• v.13 no.2
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• pp.99-120
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• 1997

Solar radiation is scattered and absorbed atmospheric compositions in the atmosphere before it reaches the surface and, then after reflected at the surface, until it reaches the satellite sensor. Therefore, consideration of the radiative transfer through the atmosphere is essential for the quantitave analysis of the satellite sensed data, specially at shortwave region. This study examined a feasibility of using radiative transfer code for estimating the atmospheric effects on satellite remote sensing data. To do this, the flux simulated by LOWTRAN7 is compared with CAGEX data in shortwave region. The CAGEX (CERES/ARM/GEWEX Experiment) data provides a dataset of (1) atmospheric soundings, aerosol optical depth and albedo, (2) ARM(Aerosol Radiation Measurement) radiation flux measured by pyrgeometers, pyrheliometer and shadow pyranometer and (3) broadband shortwave flux simulated by Fu-Liou's radiative transfer code. To simulate aerosol effect using the radiative transfer model, the aerosol optical characteristics were extracted from observed aerosol column optical depth, Spinhirne's experimental vertical distribution of scattering coefficient and D'Almeida's statistical atmospheric aerosols radiative characteristics. Simulation of LOWTRAN7 are performed on 31 sample of completely clear days. LOWTRAN's result and CAGEX data are compared on upward, downward direct, downward diffuse solar flux at the surface and upward solar flux at the top of the atmosphere(TOA). The standard errors in LOWTRAN7 simulation of the above components are within 5% except for the downward diffuse solar flux at the surface(6.9%). The results show that a large part of error in LOWTRAN7 flux simulation appeared in the diffuse component due to scattering mainly by atmispheric aerosol. For improving the accuracy of radiative transfer simulation by model, there is a need to provide better information about the radiative charateristrics of atmospheric aerosols.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.41 no.4
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• pp.347-355
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• 2021

During the earthquake, for multi-story structure, if the first floor is soft, the deformation will concentrate on that floor causing a serious damage to the column members which might leads to the collapse of the whole structure like Piloti structure during the Pohang earthquake in Korea. According to the 2016 National Disaster Management Research Institute's "Investigation of Seismic Reinforcement and Cost Analysis of Domestic Non-seismic Buildings", the rate of seismic resistance of private reinforced concrete buildings was 38.3 %. Among them, it was reported that the seismic-resistance ratio of the two to five-story structures was less than 50 %. Accordingly, the government is trying to improve the seismic rate through support projects, but the conventional seismic reinforcement methods are still expensive, and emergency construction is difficult. Therefore, in this study, the field applicability was evaluated by improving the reinforcement method using Velcro, which was developed through the research project of the Ministry of Land, Transport and Maritime Affairs in 2014. In order to improve the performance of the Velcro reinforcement method, introducing the initial tension of Velcro using high foaming rigid urethane filling between the Velcro and concrete of the columns was applied. Additionally, an experiment was conducted to evaluate the ductility of Velcro specimen from the concrete confinement effect. As a result, the ductility of the Velcro specimen was improved compare to Normal specimen. However, the energy dissipation capacity of VELCRO2 is better than VELCRO1, yet the maximum ductility of those two specimens did not show a significant difference. Therefore, the improvement of the internal filler material is still needed to have a better maximum ductility.

• Journal of Applied Biological Chemistry
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• v.61 no.4
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• pp.371-377
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• 2018

The fruits of Panax ginseng were extracted with 80% aqueous MeOH and the concentrates were partitioned into EtOAc, n-BuOH, and $H_2O$ fractions. The repeated $SiO_2$ and octadecyl $SiO_2$ column chromatographies for the EtOAc fraction led to isolation of five ginsenosides. The chemical structures of these compounds were determined as ginsenoside F1 (1), ginsenoside F2 (2), ginsenoside F3 (3), ginsenoside Ia (4), notoginsenoside Fe (5) based on spectroscopic analyses including nuclear magnetic resonance, MS, and infrared. Compounds 2-5 were isolated for the first time from the fruits of P. ginseng in this study. All isolated compounds were evaluated for cytotoxic activities against human cancer cell lines such as HCT-116, SK-OV-3, human cervix adenocarcinoma (HeLa), HepG2, and SK-MEL-5. Among them compounds 2, 4, and 5 showed significant cytotoxicity on cancer cells. Compound 2 exhibited cytotoxicity on SK-MEL-5, HepG2, and HeLa cells with $IC_{50}$ values of 82.8, 86.8, and $78.3{\mu}M$, respectively. Compound 4 showed cytotoxicity on HCT-116, SK-MEL-5, SK-OV-3, HepG2, and HeLa cells with $IC_{50}$ values of 24.5, 25.4, 26.3, 22.0, and $24.9{\mu}M$, respectively. Compound 5 did on SK-MEL-5 cell with $IC_{50}$ value of $81.7{\mu}M$. The cytotoxicity of ginsenoside 2, 4, and 5 isolated from the fruits of Panax ginseng showed strong inhibition effect against on cancer cells, all of which have a glucopyranosyl moiety on C-3.

• Journal of the Korean Geotechnical Society
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• v.26 no.7
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• pp.49-58
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• 2010

This paper presents experimental results of a series of 1-g shaking table model tests performed on end-bearing single piles and pile groups to investigate the effect of particle size on the dynamic behavior of soil-pile systems. Two soil-pile models were tested twice: first using Jumoonjin sand, and second using Australian Fine sand. In the case of single-pile models, the lateral displacement was almost within 1% of pile diameter which corresponds to the elastic range of the pile. The back-calculated p-y curves show that the subgrade reaction of the Jumoonjin-sand-model ground was larger than that of the Australian Fine-sand-model ground at the same displacement. This phenomenon means that the stress-strain behavior of Jumoonjin sand was initially stiffer than that of Australian Fine sand. This difference was also confirmed by resonant column tests and compression triaxial tests. And the single pile p-y backbone curves of the Australian fine sand were constructed and compared with those of the Jumoonjin sand. As a result, the stiffness of the p-y backbone curves of Jumunjin sand was larger than those of Australian fine sand. Therefore, using the same p-y curves regardless of particle size can lead to inaccurate results when evaluating dynamic behavior of soil-pile system. In the case of the group-pile models, the lateral displacement was much larger than the elastic range of pile movement at the same test conditions in the single-pile models. The back-calculated p-y curves in the case of group pile models were very similar in both sands because the stiffness difference between the Jumoonjin-sand-model ground and the Australian Fine-sand-model ground was not significantly large at a large strain level, where both sands showed non-linear behavior. According to a series of single pile and group pile test results, the evaluation group pile effect using the p-multiplier can lead to inaccurate results on dynamic behavior of soil-pile system.