• Journal of Embryo Transfer
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• v.13 no.3
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• pp.301-312
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• 1998

To efficiently separate a protein stimulating sperm swim-up migration and movement from follicular proteins, the effect of paper chromatography and liquid chromatography with reverse phase column and superose column on protein separation was examined. And the results obtained were as follows; 1. The band component that was separated with paper chromatography stimulated sperm migration and movement depending on its additional levels. Especially, band I component significantly increased sperm migration. But, all components of bands 1, 2 and 3 showed lower sperm migration and movement, compared to follicular fluid at the same additional level. 2. Among the components separated from follicular protein of 2~5mm follicles with reverse phase column ($\mu$RPC), components at retention time (RT) of 3.33, 7.00, 13.87, and 16.6A minutes stimulated sperm migration within a limited range. 3. All components separated from follicular protein of 10mm follicles with $\mu$RPC column didn't stimulate sperm migration and movement. 4. Among the components separated from follicular protein of 2~5m follicles with superose column, components at retention volume (RV) of 1.35 and 0.82 ml significantly stimulated sperm migration and movement. In conclusion, protein components stimulating sperm migration and movement were efficiently separated with superose column in Smart system. Especially, components of RV 1.35 and RV0.82 stimulated sperm swim-up separation.

• Biotechnology and Bioprocess Engineering:BBE
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• v.8 no.2
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• pp.117-125
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• 2003

Experimental and theoretical works were performed for the separation of large polyelectrolyte, such as DNA, in a column packed with gel particles under the influence of an electric field. Since DNA quickly orient in the field direction through the pores, this paper presents how intraparticle convection affects the residence time distribution of DNAs in the column. The concept is further illustrated with examples from solid -liquid systems, for example, from chromatography Showing how the column efficiency is improved by the use of a n electric field. Dimensionless transient mass balance equations were derived, taking into consideration both diffusion and electrophoretic convection. The separation criteria are theoretically studied using two different Peclet numbers in the fluid and solid phases. These criteria were experimentally verified using two different DNAs via electrophoretic mobility measurements. which showed how the separation position of the DNAs varies in the column in relation to the Peg/Pef values of an individual DNA. The residence time distribution was solved by an operator theory and the characteristic method to yield the column response.

• Structural Engineering and Mechanics
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• v.14 no.3
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• pp.307-330
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• 2002

This study aims at postulating a simple methodology for predicting the failure modes of monotonically loaded reinforced concrete beam-column joints. All the factors that affect the failure modes of joints are discussed in detail using an experimental database of monotonically loaded exterior beam-column joints. The relative contributions of the strut and truss mechanisms to joint shear strength are determined based on the test results. A simple design equation for the beam longitudinal reinforcement ratio for joints with low, medium and high amount of stirrups is developed. The factors influencing the failure modes of monotonically loaded exterior beam-column joints are investigated in detail. Design charts that predict the failure modes of exterior beam-column connections both with and without stirrups are developed. Experimental data are compared with the design charts. The results show that the simple methodology gives very accurate predictions of the failure modes.

• Steel and Composite Structures
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• v.20 no.4
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• pp.851-867
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• 2016

Cruciform sections are an appropriate option for columns of orthogonal moment resisting frames for equal bending strength and stiffness about two main axes and the implementation is easier for continuity plates. These columns consist of two I-shaped sections, so that one of them is cut out in middle and two generated T-shaped sections be welded into I-shaped profile. Furthermore, in steel moment frames, unbalance moment at the beam-column connection leads to shear deformation in panel zone. Most of the obtained relations for panel zone strength derived from experimental and analytical results are on I-shaped columns with almost thin flanges. In this paper, a parametric study has been carried out using Finite Element Method (FEM) with effective parameters at the panel zone behavior. These parameters consist of column flange thickness, column web thickness, and thickness of continuity plates. Additionally, a mathematical model has been suggested to determine strength of cruciform column panel zone and has been shown its accuracy and efficiency.

• Journal of Environmental Science International
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• v.10 no.6
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• pp.401-405
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• 2001

For the purpose of developing a new process f3r the elimination of oil components from oily waste water, experimental researches using oil gelling agent were performed. The process is composed of three units, that is, decantator, gelling column and adsorption column. 10g of gelling agent in the gelling column could effectively absorb 15.65g of oil from ship washing waste water and 16.93g of oil from steel industry waste water. COD in waste waters dramatically diminished not in the gelling column but in the adsorption column. The .gelling is hindered by other organic components in waste water, and the optimum space time f3r the gelling column Is 20min. 1g of gelling agent absorbed 3.7-4.0g of oil from waste waters with 25 min in the batch operation.

• Journal of The Korean Digital Architecture Interior Association
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• v.4 no.2
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• pp.44-51
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• 2004

It is generally known in seismic design that the beam yield type frames have more advantages than column yield type of which damage is likely to concentrate to any story. However we may design a building as a beam yield type, it becomes actually a column yield type collapse mode for slab floor diaphragm effect. Considering these points, the column yield type frames are selected and designed as the specimens. The object of this study is to grasp quantitatively the restoring force characteristic values and to estimate the seismic performances of column yield type steel rahmen.

• Proceedings of the Korea Concrete Institute Conference
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• 2004.05a
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• pp.692-695
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• 2004

Seismic performance of reinforced concrete(RC) column bent piers to bidirectional seismic loadings was investigated experimentally. RC column bent piers represent one of the most popular forms of piers used in highway bridges. Further to series of previous experimental researches for the performance of single bridge columns subjected to seismic loadings, four column bent piers were constructed in 400 mm diameter and 2,000 mm height. Each pier has two circular supporting columns. These piers were tested under lateral load reversals with axial load of $0.1f_{ck}A_g$. Bidirectional lateral loadings were applied. The test parameters included: different transverse reinforcement contents and lap-spliced longitudinal reinforcing steels. Test results indicate that lap-splices of longitudinal reinforcing steels have significantly influence on hysteretic response of column bent piers. Column capacity changed with the level of transverse confinement, and bidirectional repeated loadings induced more strength and stiffness degradation than unidirectional repeated loading.

• Computers and Concrete
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• v.6 no.5
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• pp.403-419
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• 2009

Short column effect is cause to failure of columns which may result in severe damages or even collapse during earthquakes. The scope of the study is mainly to reveal the effect of short column on the holistic behaviour of the buildings. The nonlinear analysis of 31 different frame buildings containing short column problem are carried out using finite element method. The finite element models were selected by 2 bays and 3 stories. Since the short columns are generally seen in the first storey of the buildings, in the study, they are only constructed in the same storey. The adverse effect of the short column on the response of buildings was shown in terms of the total load factor and displacement capacity of building. The response of buildings in terms of ground storey displacements is presented in figures and discussed. It is revealed that if the window openings are constructed along the bays, the total load capacity is decreased 85% compared with reference model in which all of bays are filled with infill walls.

• Computers and Concrete
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• v.18 no.5
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• pp.1053-1063
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• 2016

As concrete is most usable material in construction industry it's been required to improve its quality. Nowadays, nanotechnology offers the possibility of great advances in construction. For the first time, the nonlinear buckling of straight concrete columns armed with single-walled carbon nanotubes (SWCNTs) resting on foundation is investigated in the present study. The column is modelled with Euler-Bernoulli beam theory. The characteristics of the equivalent composite being determined using the Mori-Tanaka model. The foundation around the column is simulated with spring and shear layer. Employing nonlinear strains-displacements, energy methods and Hamilton's principal, the governing equations are derived. Differential quadrature method (DQM) is used in order to obtain the buckling load of structure. The influences of volume percent of SWCNTs, geometrical parameters, elastic foundation and boundary conditions on the buckling of column are investigated. Numerical results indicate that reinforcing the concrete column with SWCNTs, the structure becomes stiffer and the buckling load increases with respect to concrete column armed with steel.

• Structural Monitoring and Maintenance
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• v.7 no.3
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• pp.167-193
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• 2020

In this paper, the behavior of interior steel fiber reinforced concrete beam - column joints (BCJs) under cyclic loading is investigated. An experimental program including tests on twelve reinforced concrete (BCJs) specimens under cyclic loading was carried out. The test specimens are divided into two groups having different geometry: group (G1) (symmetrical BCJs specimens) and group (G2) (nonsymmetrical BCJs specimens). The parameters considered in this study are the steel fibers (SFs) content by volume of concrete (V_f), the spacing of shear reinforcement at the joint region, and the area of longitudinal flexural reinforcement. Test results show that the addition of 0.5% SFs with stirrups spacing S=S_max has effectively enhanced the overall performance of BCJs with respect to energy dissipation, ductility ratio, spreading and width of cracks. The failure of specimens is governed mainly by the formation of a plastic hinge at the face column and outside the beam-column junction. Secondary shear cracks were also observed in the beam-column junctions.