• Korean Journal of Microbiology
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• v.34 no.3
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• pp.132-136
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• 1998

Molecular analyses of natural microbial communities are often dependent upon the obtainments of pure nucleic acids. The four methods (elution after agarose gel electrophoresis, G-75 microspin columns, hydroxyapatite mi-crospin columns, and polyvinylpolypyrrolidone (PVPP) microspin columns) were compared for the removal of PCR-inhibitory humic substances from the crude DNA extracts of marine sediment samples. The PVPP microspin columns have shown superior removal of humic substances from the crude DNA extract of marine sediment samples, with yield of $4.8{\mu}g/g$ (dry weight of sediment). The purified DNA by this rapid method was pure enough to amplify 1.5 kb fragment corresponding almost full length of 16S rRNA genes.

• Journal of the Korean Institute of Rural Architecture
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• v.14 no.2
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• pp.1-10
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• 2012

The purpose of this study is to provide the method of saving the construction cost per unit area of the New Hanok using the inner space of a roof as a floor space with a structural modification of it's roof. The proportion of a roof of the Traditional Hanok accounts for about a half of it's elevation, so it is an essential element of it. But, compare to the whole construction cost of the Traditional Hanok, it costs over a half of expenses to build it. Recently, at the traditional building type, it is found that the inner space of a roof of it is used as a floor space with a structural modification of it's roof. It can be divided into two types, the storage type and the living type. The New Hanok at downtown can accommodate various new lifestyle, so the length of it's Yangtong is longer than that of the Traditional Hanok. When we adjust the proportion of the traditional Hanok to the New Hanok, the height of the column and the roof of the New Hanok becomes also higher than those of the traditional Hanok. So, using the upper part of the column and the roof of the New Hanok, we can make the inner space of a roof of the New Hanok as a floor space -like a bed room, tea room, personal work space and a storage- vertically connect with the 1st floor as the main living area. As a result, it is expected to save the unit construction cost of the New Hanok and has an extensity of space when we build the New Hanok at downtown.

• Journal of the Korean Society of Food Science and Nutrition
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• v.25 no.2
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• pp.255-260
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• 1996

증편반죽의 발효에 따른 해면상의 조직(망상구조)형성능의 mechanism을 조사하기 위하여 반죽의 구성성분의 경시적 물성 및 효소(diastase 및 protease) 활성의 변화, 구성 전분 분획의 glucose chain length 변화 및 1% SDS에 의해서 용출되는 단백질 분획의 변화에 대해서 검토하였다. 발효가 진행됨에 따라 증편반죽의 pH는 감소한 반면 점성 및 부피는 발효경과 10시간가지 증가하다가 그 이후로는 약간 감소하는 경향을 보였다. 발효의 진행과 더불어 diastase의 활성은 증가하였으며, 쌀전분 분획 중 amylose의 함량은 약간 감소하였다. 그리고 전분분자의 $\alpha-1,6-glucoside$ 결합을 isoamylase(debranching enzyme)로 가수분해시킨 후 Sephadex G-75를 이용한 chromatogram 분석에 의하면 쌀가루 전분 분획과 증편반죽 전분 분획의 glucose chain length 분포는 거의 유사하지만 발효가 진행됨에 따라 중합도가 낮은 부분이 우선적으로 diastase의 작용을 받은 것이라고 생각되어진다. 한편, 발효가 진행된에 따라 protease의 활성은 증가하고 있었음에도 불구하고 증편반죽의 당에 의한 단백질 분획의 고분자화가 Suprose CL-12 column chromatogram 상에서 관찰되었는데, 이것은 아마도 발효과정 중 증편반죽에 공존하는 미생물들의 발효산물인 당질(gum질)을 매개로 한 단백질 분자의 회합에 의한 결과라고 생각되어진다. 따라서 이러한 결과를 미루어 볼 때 증편반죽의 망상구조 형성능은 발효과정 중 일어나는 당과 단백질간의 상호작용에 의한 결과라 생각되어진다.

• Transactions of the Korean Society of Mechanical Engineers
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• v.15 no.6
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• pp.2147-2159
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• 1991

The effects of principal dimensions of internal mixing twin-fluid atomized and operating conditions on the atomizing characteristics are experimentally investigated. The tests are conducted over the wide range of air/liquid ratio to predict influences of the diameter and length of nozzle, contacting angle between air and liquid in the mixing chamber, and air orifice diameter on the mean drop size(SMD), spray angle, distribution of drop size, and spray dispersion, And also, initial distribution of liquid column by air stream within the mixing chamber are observed through the transparent nozzles. A He-Ne laser particle sizer(MALVERN Model 2604) was used to measure the Sauter.s mean diameter( $D_{321}$) and droplet sizes distribution. In this experiment the air/liquid ratio, mixing length and nozzle diameter have a great influence on SMD, spray angle, droplet sizes distribution and spray dispersion.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.34 no.6
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• pp.51-58
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• 2006

The spray characteristics of liquid jet injected into subsonic cross-flow were investigated experimentally. Spray trajectories were captured using CCD camera. Droplet sizes were measured using PDPA and Image Express. The nozzle diameter was 0.5 mm, and its length-to-diameter ratios (L/D) ran$4.11{\times}10^6$ged from 1.0 to 6.0. Experimental results indicate that the breakup point is delayed by increasing gas momentum ratio and the penetration length is decreased by increasing Weber number. At low injection angle(${\theta}$ < $90^{\circ}$), Weber number is dominant parameter for trajectories, but at high injection angle(${\theta}$ > $90^{\circ}$), L/D is dominant parameter for trajectories rather than Weber number.

• Journal of Soil and Groundwater Environment
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• v.22 no.3
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• pp.27-41
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• 2017

This study dealt with the characteristics and the interrelations of hydrogeological parameters such as hydraulic conductivity, dispersivity and effective porosity of unconsolidated sediments for providing the basic data necessary for the planning of the management and preservation of groundwater quality in the Nakdong River Delta of Busan City, Korea. Groundwater quality in this area has been deteriorated due to seawater intrusion, agricultural fertilizer and pesticide, industrial wastewater, and contaminated river water. The physical properties (grain size distribution, sediment type, sorting) and aquifer parameters (hydraulic conductivity, effective porosity, longitudinal dispersivity) were determined from grain size analysis, laboratory permeability test and column tracer test. Among 36 samples, there were 18 Sand (S), 7 Gravelly Sand (gS), 5 Silty Sand (zS), 5 Muddy Sand (mS), and 1 Sandy Silt (sZ). Hydraulic conductivity was determined through a falling head test, and ranged from $9.2{\times}10^{-5}$ to $2.9{\times}10^{-2}cm/sec$ (0.08 to 25.6 m/day). From breakthrough curves, dispersivity was calculated to be 0.35~3.92 cm. Also, effective porosity and average linear velocity were obtained through the column tracer test, and their values were 0.04~0.46 and 1.06E-04~6.49E-02 cm/sec, respectively. Statistical methods were used to understand the interrelations among aquifer parameters of hydraulic conductivity, effective porosity and dispersivity. The relation between dispersivity and hydraulic conductivity or effective porosity considered the sample length, because dispersivity was affected by experimental scale. The relations between dispersivity and hydraulic conductivity or effective porosity were all in inverse proportion for all long and short samples. The reason was because dispersivity was in inverse proportion to the groundwater velocity in case of steady hydrodynamic dispersion coefficient, and groundwater velocity was in proportion to the hydraulic conductivity or effective porosity. This study also elucidated that longitudinal dispersivity was dependent on the scale of column tracer test, and all hydrogeological parameters were low to high values due to the sand quantity of sediments. It is expected that the hydrogeological parameter data of sediments will be very useful for the planning of groundwater management and preservation in the Nakdong River Delta of Busan City, Korea.

• Journal of Plant Biology
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• v.37 no.2
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• pp.167-173
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• 1994

Poly(A)+ RNA was selected from Canavalia lineata root nodule RNA through oligo(dT) cellulose column and used for construction of a cDNA library using λgt10-EcoRI arms. The size of the library was 7.2$\times$105 pfu/mL. A full length leghemoglobin (Lb) cDNA clone, pCILb1(687 bp) isolated with soybean Lb probe, contained one open reading frame (ORF) of 447 bp with 54 bp plus 186 bp at 5' and 3' untranslated region, respectively. A consensus sequence of plant translation start region (AAAATGGG) was found at 5' untranslated region, and two polyadenylation-related sequence (AATAAA, AATAAG) and a conserved motif between them (gACTTGTT) were found upstream of poly(A)+ tail consisted of 13 (A)s at 3' untranslated region. The ORF encoded a polypeptide consisted of 149 amino acids with a molecular weight of 16.2 kD. Deduced amino acid sequences showed high degree of homology values with those of other Lbs ranging from 66% (Casuarina glauca) to 85% (Glycine max).

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.20 no.5
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• pp.517-522
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• 2011

Most of SEM is double condenser lens system. Two condenser lenses are required to provide the high demagnification ratios necessary for forming nanometer probes. The thin lens concept provides a highly useful basis for preliminary calculations in a broad range of situations. It is an easy way to understand the electron beam paths in column. Demagnification is easily calculated by this method. In this paper, we present design processes for condenser lens's demagnification by using thin lens combination model. Also, we verify the reliability of our design processes by comparing the modeled demagnification with these of corrected condenser lens.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 2005.05a
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• pp.30-34
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• 2005

This paper describes the design and manufacturing of a focused-ion-beam machining system which can make small features of nano size. We use a SIMION simulator in order to obtain the design data of an ion column. The simulation result shows that the focal length of ion beam decreases as the applied voltage of object lens increases. Finally, we obtained the good images of a mesh of 50 micrometers by using the adjustment of applied voltage, acceleration power, and dimension of each elements.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2003.05a
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• pp.185-191
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• 2003

A numerical study on the hydrodynamic properties of a floating flexible breakwater consisting of triple vertical porous membrane structures attached to a floating rigid pontoon restrained by moorings is carried out in the context of two-dimensional linear wave-flexible body interaction theory. The tensions in the triple membranes are achieved by hanging a clump weight from its lower ends. The clump weight is also restrained properly by moorings. The dynamic behavior of the breakwater was described through an appropriate Green function, and the fluid multi-domains are incorporated into the boundary integral equation. Numerical results are presented which illustrate the effects of the various wave and structural parameters on the efficiency of the breakwater as a barrier to wave action. It is found that the wave reflection and transmission properties of the structures depends strongly on the membrane length taking major fraction of water column, the magnitude of tensions on membrane achieving by the clump weight, proper mooring types and stiffness, the permeability on the membrane dissipating wave energy.