• Title/Summary/Keyword: colony test

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Isolation and Identification of Bacillus cereus from Fermented Red Pepper-Soybean Paste (Kochujang), and Its Heat Resistance Characteristics

  • Kim, Yong-Suk;Ahn, Yong-Sun;Jeon, Do-Youn;Shin, Dong-Hwa
    • Food Science and Biotechnology
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    • v.17 no.1
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    • pp.123-129
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    • 2008
  • To isolate Bacillus cereus presenting at a level of 5 log CFU/g in kochujang, a primary dilution ($10^{-1}$) of kochujang was heated at $85^{\circ}C$ for 5 min. Two isolated strains Voges-Proskauer positive colony (KBC) and a negative colony (KBM) were identified as B. cereus and Bacillus mycoides, respectively, by biochemical test and 16S rDNA sequencing. $D_{100^{\circ}C}$-Values of KBC and KBM strains was 8.37 and 7.08 min, respectively. When spores of KBC strain were inoculated to kochujang at the level of 4-5 log spores/g, the number of spores was no significant difference (p<0.05) for each sample from 1 up to 60 day of aging. When kochujang was inoculated with 4 log spores/g and heated at $85^{\circ}C$ for 15 min, the number of spores was similar to that of unheated kochujang. Therefore, we estimated that B. cereus isolated from kochujang resistant on the heat treatment ($85^{\circ}C$, 15 min) and its heat resistance characteristics could be used to count the number in kochtjang.

Biological Manganese Removal in Water Treatment (정수처리에서 생물학적 망간처리)

  • Kim, Berm-Soo;Yoon, Jaekyung;Ann, Hyo-Won;Kim, Chung-Hwan
    • Journal of Korean Society of Water and Wastewater
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    • v.20 no.1
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    • pp.44-52
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    • 2006
  • Bio-filtration processes using honeycomb tubes (process 1) and aeration and manganese-sand filtration (process 2) were evaluated for the biological manganese removal efficiency. The concentration of manganese at effluent was stabilized after 20days operation in process 1. It was estimated the required time for attaching and growing microorganisms to honeycomb tubes. In long term of operation periods, manganese removal efficiency was dropped for the excessively attached biofilm and manganese dioxide to honeycomb tubes. It took several days for normal operation in process 2, after that manganese removal efficiency was increased to 98% and stabilized for 1.5 years. Microorganisms in process 1 and 2 were isolated and cultured to characterize manganese-oxidizing bacteria. Among the four types of colony, light brown colony was turned blue color by leuco crystal violet spot test. Stenotropomonas genus, known as manganese-oxidizing bacteria, was identified by 16S rDNA partial sequencing analysis which was isolated in process 1 and 2. For the biological treatment to remove manganese, these two considerations are important. One is to choose the proper media attaching manganese oxidant, another one is to define the cultural condition of isolated manganese-oxidizing bacteria.

Estimation of BOD in wastewater treatment plant by using different ANN algorithms

  • BAKI, Osman Tugrul;ARAS, Egemen
    • Membrane and Water Treatment
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    • v.9 no.6
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    • pp.455-462
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    • 2018
  • The measurement and monitoring of the biochemical oxygen demand (BOD) play an important role in the planning and operation of wastewater treatment plants. The most basic method for determining biochemical oxygen demand is direct measurement. However, this method is both expensive and takes a long time. A five-day period is required to determine the biochemical oxygen demand. This study has been carried out in a wastewater treatment plant in Turkey (Hurma WWTP) in order to estimate the biochemical oxygen demand a shorter time and with a lower cost. Estimation was performed using artificial neural network (ANN) method. There are three different methods in the training of artificial neural networks, respectively, multi-layered (ML-ANN), teaching learning based algorithm (TLBO-ANN) and artificial bee colony algorithm (ABC-ANN). The input flow (Q), wastewater temperature (t), pH, chemical oxygen demand (COD), suspended sediment (SS), total phosphorus (tP), total nitrogen (tN), and electrical conductivity of wastewater (EC) are used as the input parameters to estimate the BOD. The root mean squared error (RMSE) and the mean absolute error (MAE) values were used in evaluating performance criteria for each model. As a result of the general evaluation, the ML-ANN method provided the best estimation results both training and test series with 0.8924 and 0.8442 determination coefficient, respectively.

High Temperature Deformation Behavior of Ti-Al Intermetallic Compound and Orientation Distribution of Lamellae Structure (Ti-Al금속간화합물의고온변형거동및라멜라조직의결정방위분포)

  • Park Kyu-Seop;Kang Chang-Yong;Lee Keun-Jin;Chung Han-Shik;Jung Young-Guan;Fukutomi Hiroshi
    • Journal of the Korean Society for Precision Engineering
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    • v.21 no.10
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    • pp.162-169
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    • 2004
  • High temperature uniaxial compression tests in the alpha single phase region were carried out on the Ti -43mo1%Al intermetallic compound, in order to obtain oriented lamellar microstructure. The compression deformation temperatures and strain rates are from 1573k to 1623k and 1.0x10$^{-4}$ s to 5.0x10$^{-3}$ s, respectively. Fully lamellar microstructure was observed after the uniaxial compression deformation in a single phase region followed by cooling to room temperature. Lamellar colony diameter depended on strain rates and test temperatures. The diameter varied between 8601m and 300fm. Stress-strain curve showed a work softening and the size of lamellar colony diameter varied depending on peak stresses. This shows the occurrence of dynamic recrystallization. Texture measurements after the uniaxial compression deformation, showed the development of fiber during dynamic recrystallization. It is seen that the area for the maximum pole density existed in 35 degrees away from the compression plane. The texture sharpens with a decrease in strain rate

Effects of Chitosan Treatment on Properties of Wool Fabrics (키토산 가공이 모직물의 특성에 미치는 효과)

  • 모태화;이혜자;유혜자
    • Journal of the Korean Society of Clothing and Textiles
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    • v.24 no.1
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    • pp.87-95
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    • 2000
  • The changes on shrinkage, dyeability, antimicrobial activities and other physical properties of wool fabrics by chitosan treatment were investigated. A acid dye, a basic dye and natural artemisia were used for dyeing of wool fabrics. For Antimicrobial test of wool fabrics, S.Aureus was used. As the results, The wool fabrics treated with chitosan showed a marked decrease in shrinkage. Dyeability increased slightly as concentration of chitosan treatment increased, Dyeability of acid dye was the highest than other dyes. On the other hand, the dyeability of basic dye on chitosan treated fabrics was lower than untreated fabrics. Reduction rates of colony of chitosan-treated wool fabrics showed from 64.8% to 85.24% as chitosan concentration increased. Reduction rates of colony of the chitosan-treated fabrics decreased by dyeing with acid and basic dye. But increased by retreating with chitosan. Fabrics dyeing with atremisia showed above 90% reduction rate against S.Aureus on all chitosan concentrations. The Tensile properties of chitosan-treated fabrics showed little decrease. The moisture regain and the air permeability was decreased on chitosan concentration on 0.1% but recovered as chitosan concentration increased. The warmth retention was increased a little by chitosan treatment. The drapeability became hard as chitosan concentration increased.

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Influence of moisture content on mold development in flue-cured tobacco during storage (저장중 황색종 잎담배의 수분 함량이 곰팡이 발생에 미치는 영향)

  • 조대휘;안동명;민영근;이완남;이경구;이상하
    • Journal of the Korean Society of Tobacco Science
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    • v.11 no.2
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    • pp.241-246
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    • 1989
  • Mold development on leaf tobacco(Flue-cured, var. NC 82) during the storage was examined according to the initial moisture content(MC) of the tobacco. The initial moisture content of the leaf tobacco was controlled as 12,14,16% after redrying. Those were packed in cardboard boxes and stored in a warehouse. Samples were taken monthly from June of 1988 through August of 1989 and were evaluated for mold development (colony count) and MC. The moisture content change in the leaf tobacco of 12, 14 and 16% initial MC was in the range of 11.1-13.3, 12.8-15.3 and 14.9-16.7% and the mean number of colonies from them were 1.4$\times$102, 1.0$\times$102, and 4.0$\times$102 colonies per gram of tobacco, respectively, however mold-damaged leaf tobacco other than the treatments was occasionally observed to have the colony number as high as 2.3$\times$105 colonies per gram of tobacco. In the meantime, the leaf tobacco was stored at 6 relative humidity levels at $25^{\circ}C$ in laboratory test. Leaf tobacco of 15.0-16.4% MC was maintained without the mold-damage after 80 days of storage whereas those of 19.9, 21.3, 25.5 and 27.1% MC became moldy after 50, 15, 11 and 6 days of storage, respectively.

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Foraging Habitat Preferences of Herons and Egrets

  • Choi, Yu-Seong;Kwon, In-Ki;Yoo, Jeong-Chil
    • Journal of Ecology and Environment
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    • v.30 no.3
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    • pp.237-244
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    • 2007
  • We investigated the foraging habitat preferences of herons and egrets in an agricultural area in Asan city, Chungcheongnam-do, Korea. In the study area, rice fields were the most abundant habitat type (86.8%) and total suitable feeding habitat was greater in the northern area (59.0%) than the southern area (22.5%) of the colony. Most feeding herons and egrets were located in the northern area of the colony. The number of feeding individuals in a given area was related to the available feeding area (Pearson correlation, r=0.773, p<0.001 for field habitats; r=0.901, p<0.001 for freshwater habitats). Feeding habitat preferences differed among species. Grey herons (Ardea cinerea), great egrets (Egretta alba), and black-crowned night herons (Nycticorax nycticorax) used reservoirs and ditches. However, intermediate egrets (E. intermedia) and cattle egrets (Bubulcus ibis) were dependent on rice fields. The little egret (E. garzetta) was a habitat generalist using all types of habitats. The two largest species, grey herons and great egrets, fed at deeper site than little egrets and foraged in deeper sites in reservoirs than in ditches (${\chi}^2-test,\;{\chi}^2{_3}=26.6$ and p<0.001 for grey herons, ${\chi}^2{_3}=17.5$ and p<0.001 for great egrets). All species displayed seasonal changes in feeding habitat use and these changes were related with changes in availability of feeding habitats.

Application of an Optimized Support Vector Regression Algorithm in Short-Term Traffic Flow Prediction

  • Ruibo, Ai;Cheng, Li;Na, Li
    • Journal of Information Processing Systems
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    • v.18 no.6
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    • pp.719-728
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    • 2022
  • The prediction of short-term traffic flow is the theoretical basis of intelligent transportation as well as the key technology in traffic flow induction systems. The research on short-term traffic flow prediction has showed the considerable social value. At present, the support vector regression (SVR) intelligent prediction model that is suitable for small samples has been applied in this domain. Aiming at parameter selection difficulty and prediction accuracy improvement, the artificial bee colony (ABC) is adopted in optimizing SVR parameters, which is referred to as the ABC-SVR algorithm in the paper. The simulation experiments are carried out by comparing the ABC-SVR algorithm with SVR algorithm, and the feasibility of the proposed ABC-SVR algorithm is verified by result analysis. Continuously, the simulation experiments are carried out by comparing the ABC-SVR algorithm with particle swarm optimization SVR (PSO-SVR) algorithm and genetic optimization SVR (GA-SVR) algorithm, and a better optimization effect has been attained by simulation experiments and verified by statistical test. Simultaneously, the simulation experiments are carried out by comparing the ABC-SVR algorithm and wavelet neural network time series (WNN-TS) algorithm, and the prediction accuracy of the proposed ABC-SVR algorithm is improved and satisfactory prediction effects have been obtained.

Antibacterial Properties of Silver-alginate/PVP Nanofiber (은-알지네이트/PVP 나노섬유의 항균 특성)

  • Choi, Yoo-Sung;Min, Kyung-Du;Yoon, Doo-Soo
    • Applied Chemistry for Engineering
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    • v.24 no.4
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    • pp.416-422
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    • 2013
  • In order to incorporate silver ions into the alginate, silver-alginate was prepared with aqueous solutions of silver nitrate. In the study, the silver-alginate was prepared by blending with poly vinylpyrrolidone solutions and the electrospinning was performed by using this blend solution. Antibacterial properties of silver-alginate/PVP solutions were estimated for Escherichia coli and Staphylococcus aureus by the colony counting test. Electrospinning conditions of silver-alginate/PVP solution were the tip-to-collector distance of 22 cm, the flow rate of the solution at 0.01 mL/min, and the voltage at 26 kV. The form and size of silver-alginate/PVP nanofibers were estimated by SEM and Image J. The average diameter of the electrospun SA5P15 fibers was 124 nm and showed a narrow diameter distribution. The reduction of bacteria for SA5P15 exhibited 99.9% after 24 h.

Antimicrobial Effects Of Herbs For Removing Dampness And Promoting Urination Against Vaginal Microbe (이수삼습약(利水參濕藥)의 질내(膣內) 미생물(微生物)에 대한 항균효과(抗菌效果))

  • Lee, Jin-Moo;Lee, Chang-Hoon;Cho, Jung-Hoon;Jang, Jun-Bock;Lee, Kyung-Sup;Kim, Eun-Sook
    • The Journal of Korean Obstetrics and Gynecology
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    • v.20 no.1
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    • pp.1-15
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    • 2007
  • Purpose : This study was conducted to investigate the antimicrobial effects of herb for removing dampness and promoting urination against vaginal microbes. Methods : Staphylococcus aureus. Methicillin-resistant Staphylococcus aureus(MRSA), Candida albicans and Gardnerella vaginalis were used for vaginitis-induced microbes. Lactobacillus gasseri, Streptococcus spp. and Escherichia coil HB101 were used for normal vaginal florae. And herbs for removing dampness and promoting urination(Dianthi herbs. Tokoro Rhizoma, Saururi Herbs, Pyrrosiae Folium, Artemisiae Iwayomogii Herba, Plantaginis Semen, Tetrapanacis Medulla, Polygoni Avicularis Herba, Malvae Semen, Akebiae Caulis, Kochiae Fructus, Lygodii Spora) were used. Antimicrobial activities were estimated by the change of optical densities and colony test in vitro. Results : Plantaginis Semen, Artemisiae Iwayomogii Herba and Lygodii Spora had the antimicrobial susceptibility and selective toxicity against MRSA and Gardnerella vaginalis. Polygoni Avicularis Herba had the antimicrobial susceptibility and selective toxicity against Staphylococcus aureus. MRSA and Gardnerella vaginalis. Malvae Semen and Kochiae Fructus had the antimicrobial susceptibility and selective toxicity against MRSA. Dianthi Herba had the antimicrobial susceptibility and selective toxicity against Gardnerella vaginalis. Conclusion : According to these results, we can suggest that Plantaginis Semen, Artemisiae Iwayomogii Herba, Lygodii Spora, Polygoni Avicularis Herba, Malvae Semen, Kochiae Fructus and Dianthi Herba would be available to the antimicrobial agent for vaginitis-induced microbe in vitro.

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