• Title/Summary/Keyword: colony test

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A Study of the Efficacy to Control the Bacteria Colony of Scalp in Ayurvedic Oils on Clinical Testing

  • Choi, Jung-Myung
    • Journal of Fashion Business
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    • v.13 no.6
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    • pp.45-60
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    • 2009
  • This study examined the efficacy of restraining the increase of the bacteria colony of scalp according to the application of the Ayurvedic base oils and essential oils to scalp and hair. The result is as follows. The experiment illustrated that as the result of doing the paired sample t-test of a treated group and a control group according to constitutional oiling, the efficacy of the statistically significant decrease of the colony manifested in six tested groups except the group of Pitta constitution to which sesame and lavender were applied. Also, in a group that sesame was applied to Vata constitution and a group that coconut and lavender were applied to Pitta constitution, according to the passage of time, the result of being reduced the number of the colony was obtained and it showed that the oiling suitable for each constitution has the efficacy to decrease the colony of scalp. At the same time, regardless of the constitutions, the application of oils also showed the efficacy of restraining the increase of the bacteria colony of scalp. As the result of performing Paired Sample t-test for the subjective evaluation of the subjects in pre and post clinical testing, In the analysis of questionnaire that were obtained before and after the clinical testing, the results of all of the items except the item questioning a degree of inflammation appeared to be significant. That is to say, the subjects answered that a degree of keratin or a pain in scalp, an amount of sebum and a degree of hair damage were decreased after the application of oil more than before it. And in the question of a degree of hair damage, the application of oils were proved as having the efficacy to improve the hair damage.

Pre-validation of Colony Forming Efficiency Assay for Assessing the Cytotoxicity of Nanomaterials (나노물질의 세포독성 평가법으로 Colony Forming Efficiency Assay에 대한 검증연구)

  • Jo, Eunhye;Lee, Jaewoo;Park, Sun-Young;Kim, Pilje;Choi, Kyunghee;Eom, Igchun
    • Journal of Environmental Health Sciences
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    • v.41 no.1
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    • pp.17-23
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    • 2015
  • Objectives: The cytotoxcities of Au, Ag, SWCNT, $SiO_2$, and ZnO nanomaterials were evaluated in order to assess their potential toxicological effects in in vitro cell models using colony forming efficiency (CFE) assay. Methods: The CFE assay of the test materials was carried out on Hep G2 cells. The size distribution of nanomaterials was studied by transmission electron microscopy (TEM). Changes in cell viability after treatment with a toxicant will result in a decreased number of colonies formed in comparison to solvent. Results: The TEM images show that all the particles except SWCNT and ZnO can be considered approximately spherical. The gold and $SiO_2$ nanoparticles show no response (no toxicity) in concentration response experiments. A statistically significant toxic effect was found in Hep G2 cells treated with Ag, SWCNT and ZnO nanomaterials. Conclusion: In this study, we considered CFE assay to be a promising test for screening studies for cytotoxicity with physicochemical analysis.

A Comprehensive Cash Management Model for Construction Projects Using Ant Colony Optimization

  • Mohamed Abdel-Raheem;Maged E. Georgy;Moheeb Ibrahim
    • International conference on construction engineering and project management
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    • 2013.01a
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    • pp.243-251
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    • 2013
  • Cash management is a major concern for all contractors in the construction industry. It is arguable that cash is the most critical resource of all. A contractor needs to secure sufficient funds to navigate the project to the end, while keeping an eye on maximizing profits along the way. Past research attempted to address such topic via developing models to tackle the time-cost tradeoff problem, cash flow forecasting, and cash flow management. Yet, little was done to integrate the three aspects of cash management together. This paper, as such, presents a comprehensive model that integrates the time-cost tradeoff problem, cash flow management, and cash flow forecasting. First, the model determines the project optimal completion time by considering the different alternative construction methods available for executing project activities. Second, it investigates different funding alternatives and proposes a project-level cash management plan. Two funding alternatives are considered; they are borrowing and company own financing. The model was built as a combinatorial optimization model that utilizes ant colony search capabilities. The model also utilizes Microsoft Project software and spreadsheets to maintain an environment that incorporates activities, their durations, and other project data, in order to estimate project completion time and cost. Ant Colony Optimization algorithm was coded as a Macro program using VBA. Finally, an example project was used to test the developed model, where it acted reliably in maximizing the contractor's profit in the test project.

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The Study on Rough Colony Type Mutant of Pseudomoms mori(Boyer et Lambert) Stevens, caused Mulberry Bacterial Blight: Pathogenicity and General Characteristics (뽕나무 세균성위축병균 Pseudomonas mori (Boyer et Lambert) STEVENS의 Rough Colony Type Mutant에 관한 연구 ; 병원성 및 일반적 성질)

  • Yi Young Keun;Kim Jong Wan;Cho Yong Sup
    • Korean journal of applied entomology
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    • v.16 no.1 s.30
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    • pp.21-31
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    • 1977
  • The study has been carried out to compare the pathogenicity, physiological characteristics and genetic reliability between rough colony type strain and smooth colony type strains of Pseudomonas mori (Boyer et Lambert) Stevens which were isolated from diseased plant parts in 5 different areas throughout country. The results are summarized as follow. 1. The rough colony type strain showed more agressive reactions to tested host plant varieties than smooth colony type strains though there was no differences in the appearence of lesion types caused by both strains. 2. Both colony types were differentiated morphologically in that the rough colony type strain was having more than 200r long filamentous body without flagella where as the smooth colony type strains have short rods with one or several polar flagella. 3. The colony of smooth type strains was circular, entire, smooth and opaque, while the rough type strain shelved endulated, irregular margin, rough and wrinkled colony on nutrient agar media. 4. There were no differences between both colony types in the physiological and serological test. 5. Both of smooth and rough colony type strains showed genetic reliability through more than 100 succeeding cultures on the media, and were stable to various chemicals such as 1 to 3 percent of NaCl, 5 kinds of organic acid and 4 kinds of antibiotics.

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Antibiotic effects of Medicinals resolving dampness with aroma on vaginal microorganisms (방향화습약(芳香化濕藥)이 질내(膣內) 미생물(微生物)에 미치는 영향)

  • Lee, Chang-Hoon;Cho, Jung-Hoon;Jang, Jun-Bock;Lee, Kyung-Sub;Kim, Kyung-Sook
    • The Journal of Korean Obstetrics and Gynecology
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    • v.19 no.4
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    • pp.33-46
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    • 2006
  • Purpose : This study was conducted to investigate effects of 7 herbs among medicinals resolving dampness with aroma on vaginal microorganisms. Methods : Staphylococcus aureus, Methicillin- resistant Staphylococcus aureus, Candida albicans and Gardnerella vaginalis were used for vaginal pathogenic microorganisms. Lactobacillus gasseri, Streptococcus spp. and Escherichia coli HB101 were used for vaginal flora. Medicinals resolving dampness with aroma, Pogostemonis Herba, Amomi Cardamomi Fructus, Amomi Semen, Atractylodis Rhizoma, Ammomi Tsao-ko Fructus, Alpiniae Katsumadaii Semen and Magnoliae Cortex were used in this study. In vitro antimicrobial activities were observed by optical density and colony test. Results : The optical density showed that Alpiniae Katsumadaii Semen and Magnoliae Cortex among medicinals resolving dampness with aroma had antimicrobial effects on pathogenic vaginal microorganisms (Methicillin-resistant Staphylococcus aureus). Pogostemonis Herba, Ammomi Tsao-ko Fructus had antimicrobial effects on Gardnerella vaginalis. The colony test showed that Alpiniae Katsumadaii Semen, Magnoliae Cortex among medicinals resolving dampness with aroma had antimicrobial effects on Methicillin-resistant Staphylococcus aureus. Pogostemonis Herbs, Alpiniae Katsumadaii Semen had antimicrobial effects aganist Gardnerella vaginalis. The optical density showed that Magnoliae Cortex among medicinals resolving dampness with aroma had antimicrobial effect on normal vaginal microorganisms (Streptococcus spp.). The colony test showed that medicinals resolving dampness with aroma had no antimicrobial effect on normal vaginal microorganisms. Conclusion : From this study, we could suggest that Pogostemonis Herba, Ammomi Tsao-ko Fructus, Alpiniae Katsumadaii Semen, Magnoliae Cortex of medicinals resolving dampness with aroma are available to antimicrobial agent of pathogenic vaginal microorganisms in vitro.

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Antimicrobial effects of Astringent medicinals against vaginal microbe (수삽약(收澁藥)이 질내(膣內) 징생물(徵生物)에 미치는 영향(影響))

  • Lee, Chang-Hoon;Cho, Jung-Hoon;Jang, Jun-Bock;Lee, Kyung-Sub;Yoon, Young-Jin
    • The Journal of Korean Obstetrics and Gynecology
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    • v.19 no.2
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    • pp.127-140
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    • 2006
  • Purpose : This study was conducted to investigate the antimicrobial effects of Astringent medicinals against vaginal microbe. Methods : Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, Candida albicans and Gardnerella vaginalis were used for vaginitis-induced microbe. Lactobacillus gasseri, Streptococcus spp. and Escherichia coli HB101 were used for normal vaginal florae. And Astringent medicinals (Rubi Fructus, Tritici Immatri Semen, Corni Fructus, Nelumbinis Semen, Mume Fructus, Schizandrae Fructus, Ailanthi Radicis Cortex, Galla Rhois, Myristicae Semen, Terminariae Fructus, Rosae laevigatae Fructus, Ephedrae Radix) were used in this study. Antimicrobial activities were tested by optical density and colony test in vitro. And then wee valuated the antimicrobial effects in comparison with optical density and colony test. Results : The optical density and colony test showed that Terminariae Fructus and Galla Rhois among Astringent medicinals had the antimicrobial effects. Teminariae Fructus had the antimicrobial susceptibility and selective toxicity against Candida albicans and Gardnerella vaginalis of vaginitis-induced microbe. Galla Rhois had the antimicrobial susceptibility and selective toxicity against Staphylococus aureus, Methicillin-resistant Staphylococus aureus, Candida albicans and Gardnerella vaginalis of vaginitis-induced microbe. Conclusion : According to these results, we can suggest that Terminariae Fructus and Galla Rhois among Astringent medicinals be available to the antimicrobial agent of vaginitis-induced microbe in vitro.

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A Max-Min Ant Colony Optimization for Undirected Steiner Tree Problem in Graphs (스타이너 트리 문제를 위한 Mar-Min Ant Colony Optimization)

  • Seo, Min-Seok;Kim, Dae-Cheol
    • Korean Management Science Review
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    • v.26 no.1
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    • pp.65-76
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    • 2009
  • The undirected Steiner tree problem in graphs is known to be NP-hard. The objective of this problem is to find a shortest tree containing a subset of nodes, called terminal nodes. This paper proposes a method based on a two-step procedure to solve this problem efficiently. In the first step. graph reduction rules eliminate useless nodes and edges which do not contribute to make an optimal solution. In the second step, a max-min ant colony optimization combined with Prim's algorithm is developed to solve the reduced problem. The proposed algorithm is tested in the sets of standard test problems. The results show that the algorithm efficiently presents very correct solutions to the benchmark problems.

Biological Control of Powdery Mildew by Antibiotic-producing Microorganisms Antagonistic to Erysiphe graminis

  • Lee, Yong-Se;Wolf, G.
    • Journal of Microbiology and Biotechnology
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    • v.5 no.6
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    • pp.341-345
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    • 1995
  • Seventy four microorganisms, which have antagonistic activity against to Fusarium culmorum, were tested for their inhibitory effect on colony development of obligate biotroph Erysiphe graminis f. sp. hordei Marchal, the causal agent of powdery mildew on barley plants. Of these, 13 actinomycetes isolates were shown to reduce the colony development of mildew completely by application of their 10% cell-free culture filtrates on barley leaves. An Isolate, A252, was the most powerful antagonist and its antifungal activity was further assessed. The colony development of mildew was significantly reduced by application of the 1% cell-free culture filtrate of isolate A252. In comparison to the control, the protective and curative application of 10% cell-free culture filtrate from A252 showed 88.5% and 96.1% reduction of colony numbers respectively. By the protective application, 68.3% of the inhibition was observed after 9 days of treatment, thus showed prolonged inhibitory effect. In vitro test, complete inhibition of the mycelial growth of Microdochium nivale was achieved by the treatment of 1% A252 culture filtrate and 80.2% of inhibition was observed by the 0.1% treatment.

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Controlling Sacbrood Virus Disease in Apis cerana Colonies with Biological Methods in Korea

  • Vung, Nguyen Ngoc;Kim, Iksoo;Lee, Man Young;Kim, Hye Kyung;Kim, Dong Won;Choi, Yong Soo
    • Journal of Apiculture
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    • v.33 no.4
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    • pp.283-295
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    • 2018
  • As Sacbrood virus (SBV), a causative agent of larval death and colony collapse in Apis cerana honey bee, is prevalent and poses one of the most significant threats to the Korean apiculture, development of methods to counter this viral disease is urgently needed. In this study we tested some SBV controlling methods, such as requeen, shook swam, adding Apis mellifera and spraying yogurt to SBV inoculated colony. Colony size measured by number of sealed brood and adult were evaluated every 15 days until two months while instances of recurrence were recorded up to five months after applying treatment methods. We also test the effects of yogurt on healthy and SBV-infected larvae at both in vitro reared larvae and colony level. Our result showed that all SBV controlling methods had similar success rates with respect to elimination of SBV clinical symptom up to 30 days post treatment. Mix-species and spraying yogurt method had similar pattern of sealed brood and adult number and higher than that of other SBV controlling methods up to 45 days post treatment. These two groups also showed the lower percentage of SBV recurrence (50% and 66.7%) at 120 days post treatment than other group that mostly colony had clinical symptom. Result on in vitro reared larvae challenged with yogurt showed that yogurt have neither harmless on healthy larvae nor remedial effect on SBV infected larvae. However, at colony level, colony in group received yogurt treatment removed significantly more SBV-infected larvae, SBV-killed larvae, and even healthy larvae in comparison to the control, suggested that yogurt could trigger the hygienic behavior of nurse bee. Our results recommended that it is practical in beekeeping by adding A. mellifera and spraying yogurt to control SBV in A. cerana colony.

A STUDY ON THE RELIABILITY OF THE OPTICAL CARIES ACTIVITY TEST (광학적 치아우식활성 검사법의 신뢰도에 관한 연구)

  • Park, Cheol-Hong;Lee, Nan-Young;Lee, Sang-Ho
    • Journal of the korean academy of Pediatric Dentistry
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    • v.33 no.4
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    • pp.615-623
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    • 2006
  • The purpose of this study was to evaluate the specificity, sensitivity, and diagnostic power of caries activity test using LED fluorescence. The subjects of this study were 55 children of $6{\sim}7$ years old. LED light were irradiated to labial or buccal surface of all teeth. Fluorescence from initial carious lesion of teeth illuminated by an LED light was observed through barrier filter and the number of teeth showing lesion, size and position of lesion were counted. Streptococcus mutans colony counting and dDfFtT rate test were also done and their correlation was compared. And then specificity, sensitivity, diagnostic power of optical caries activity test using LED light were evaluated. 1. There was positive $correlation({\gamma}=0.43)$ between LED fluorescence test and Streptococcus mutans count(P<0.05). 2. When visual examination was defined to standard testing method, the specificity, sensitivity, diagnostic power of LED fluorescence test were 100%, 76.1%, and 100%. 3. When dDfFtT rate was defined to standard testing method, the specificity, sensitivity, diagnostic power of LED fluorescence test were 88.9%, 47.8%, and 95.7%. 4. When S. mutans colony counting was defined to standard testing method, the specificity, sensitivity, diagnostic power of LED fluorescence test were 100%, 58.7%, and 100%. Considering the above results, optical caries activity test using LED light could be regarded as a practical method because of its close relationship with microbiological caries activity test.

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