Browse > Article
http://dx.doi.org/10.5668/JEHS.2015.41.1.17

Pre-validation of Colony Forming Efficiency Assay for Assessing the Cytotoxicity of Nanomaterials  

Jo, Eunhye (National Institute of Environmental Research)
Lee, Jaewoo (National Institute of Environmental Research)
Park, Sun-Young (National Institute of Environmental Research)
Kim, Pilje (National Institute of Environmental Research)
Choi, Kyunghee (National Institute of Environmental Research)
Eom, Igchun (National Institute of Environmental Research)
Publication Information
Journal of Environmental Health Sciences / v.41, no.1, 2015 , pp. 17-23 More about this Journal
Abstract
Objectives: The cytotoxcities of Au, Ag, SWCNT, $SiO_2$, and ZnO nanomaterials were evaluated in order to assess their potential toxicological effects in in vitro cell models using colony forming efficiency (CFE) assay. Methods: The CFE assay of the test materials was carried out on Hep G2 cells. The size distribution of nanomaterials was studied by transmission electron microscopy (TEM). Changes in cell viability after treatment with a toxicant will result in a decreased number of colonies formed in comparison to solvent. Results: The TEM images show that all the particles except SWCNT and ZnO can be considered approximately spherical. The gold and $SiO_2$ nanoparticles show no response (no toxicity) in concentration response experiments. A statistically significant toxic effect was found in Hep G2 cells treated with Ag, SWCNT and ZnO nanomaterials. Conclusion: In this study, we considered CFE assay to be a promising test for screening studies for cytotoxicity with physicochemical analysis.
Keywords
Colony forming efficiency assay; Cytotoxicity; Nanomaterials;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Ministry of Environment. 2014 Hazard Assessment Guidance of Manufacture Nanomaterials. Available: http://lib.me.go.kr:8018/search/DetailView.ax?sid=1&cid=5583017 [accessed 4 December 2014]
2 OECD. Guidance manual for the testing manufactured nanomaterials: OECD's sponsorship programme; first revision. Available: http://www.oecd.org/officialdocuments/publicdisplaydocumentpdf/?cote=env/jm/mono(2009)20/rev&doclanguage=en [accessed 4 December 2014]
3 Ceriotti L, Ponti J, Broggi F, Kobc A, Drechsler S, Thedinga E, et al. Real-time assessment of cytotoxicity by impedance measurement on a 96-well plate. Sensors and Actuators B. 2007; 123(2): 769-778.   DOI
4 Oberdorster G, Oberdorster E, Oberdorster J. Nanotoxicology: an emerging discipline evolving from studies of ultrafine particles. Environ Health Perspect. 2005; 113(7): 823-839.   DOI
5 Kim H, Park k. Excretion, tissue distribution and toxicities of titanium oxide nanoparticles in rats after oral administration over five consecutive days. J Environ Health Sci. 2013; 39(4): 369-375.
6 Jones C, Grainger DW. In vitro assessments of nanomaterial toxicity. Adv Drug Deliv Rev. 2009; 61(6): 438-456.   DOI
7 Stren ST, McNeil SE. Nanotechnology Safety Concerns Revisited. Toxicol Sci. 2008; 101(1): 4-21.   DOI
8 ISO. ISO 10993-1:2009 Biological Evaluation of Medical Devices -- Part 5: Tests for in vitro cytotoxicity. Available: http://iso.org/iso/catalogue_detail.htm?csnumber=36406 [accessed 4 December 2014]
9 OECD. Nanosafety at the OECD. Available: http://www.oecd.org/science/nanosafety/47104296.pdf [accessed 4 December 2014]
10 Bar-Ilan O, Albrecht RM, Fako VE, Furgeson DY. Toxicity assessments of multisized gold and silver nanoparticles in zebrafish embryos. Small. 2009; 5(16): 1897-1910.   DOI
11 Castiglioni S, Caspani C, Cazzaniga A, Maier JA. Short- and long- term effects of silver nanoparticles on human microvascular endothelial cells. World J Biol Chem. 2014; 5(4): 457-464.   DOI
12 Herzog E, Casey A, Lyng FM, Chambers G, Byrne HJ, Davoren M. A new approach to the toxicity testing of carbon-based nanomaterials-the clonogenic assay. Toxicol Lett. 2007; 174(1-3): 49-60.   DOI
13 Monteiro-Riviere NA, Inman AO, Zhang LW. Limitations and relative utility of screening assays to assess engineered nanoparticles toxicity in a human cell line. Toxicol Appl Pharmacol. 2009; 234(2): 222-235.   DOI
14 De Angelis I, Barone F, Zijno A, Bizzarri L, Russo MT, Pozzi R, et al. Comparative study of ZnO and TiO(2) nanoparticles: physicochemical characterisation and toxicological effects on human colon carcinoma cells. Nanotoxicology. 2013; 7(8): 1361-1372.   DOI
15 Ponti J, Colonato R, Rauscher H, Gloria S, Broggi F, Franchini F, et al. Coloy Forming Efficiency and microscopy analysis of multi-wall carbon nanotubes cell interaction. Tox Letters. 2010; 197(1): 29-37.   DOI
16 Casey A, Herzog E, Davoren M, Lyng FM, Byrne HJ, Chambers G. Spectroscopic analysis conforms the interactions between single walled carbon annotates and various dyes commonly used to assess cytotoxicity. Carbon. 2007; 45: 1425-1432.   DOI
17 EU JRC. EURL ECVAM Recommendation on the Cell Transformation Assay based on the Bhas 42 cell line. Available: https://eurl-ecvam.jrc.ec.europa.eu/eurl-ecvam-recommendations/files-bhas/EURL_ECVAM_Recommendation_Bhas-CTA_2013.pdf [accessed 4 December 2014]