• Biomolecules & Therapeutics
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• 제2권3호
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• pp.292-297
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• 1994

This study was conducted to investigate antigenic potential of DA-3030, a recombinant human granulocyte-colony stimulating factor, in guinea pigs and mice. In the active systemic anaphylaxis test, the guinea pigs sensitized with 1.25 or 12.5 $\mu\textrm{g}$/head of DA-3030 alone did not show any anaphylactic reaction. In the homologous passive cutaneous anaphylaxis reaction, anti-DA-3030 antibody was not detected in guinea pigs sensitized with 1.25 or 12.5 $\mu\textrm{g}$/head of DA-3030 alone. On the other hand, the guinea pigs sensitized with 12.5 $\mu\textrm{g}$/heed of DA-3030 incorporated in Freund's complete adjuvant(FCA) or 1 mg/head of ovalbumin incorporated in FCA showed anaphylactic reaction. Anti-DA-3030 antibody was also detected in those guinea pigs. In immunodiffusion test using the sera sensitized with DA-3030 incorporated in FCA, precipitating antibodies were detected only in the sera sensitized with DA-3030 or DA-3030 incorporated in FCA showed. In 24-hour heterologous PCA reaction with sera of C57BL/6 mice immunized with 1.25 or 12.5 $\mu\textrm{g}$/head of DA-3030 alone, none of the sera showed positive reaction. But sera of the animals immunized with 12.5 $\mu\textrm{g}$/head of DA-3030 incorporated in aluminum hydroxide gel(Alum) or 5 $\mu\textrm{g}$/head of ovalbumin incorporated in alum showed positive PCA reaction. DA-3030 did not cause anaphylactic shock or passive cutaneous anaphylaxis in guinea pigs and mice when given alone although DA-3030 incorporated in FCA or Alum induced anaphylactic shock and passive cutaneous anaphylaxis. From these results, it may be concluded the DA-3030 does not induce systemic allergic reaction when administered alone in its clinical use.

• 한국물환경학회지
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• 제23권2호
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• pp.179-183
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• 2007

This study was carried out to evaluate water quality based on physicochemical properties, including ammonium nitrogen ($NH_3-N^+$), nitrate nitrogen ($NO_3-N^-$), consumption of $KMnO_4$ and microbial indicators, such as total colony counts and total surveyed a group of 30 samples three times in 2005 (January to March, July to September, October to December). The mean concentration values of ammonium nitrogen ($NH_3-N^+$), nitrate nitrogen ($NO_3-N^-$) and the consumption of $KMnO_4$, were 0.01 mg/L (range 0.00~0.09 mg/L), 0.48 mg/L (range 0.00~3.31 mg/L) and 0.61 mg/L (0.0~3.42 mg/L), respectively. Based on the results, total colony count was detected in the ranges of 0~412 CFU/mL. In details, 84.4% of test samples was ${\leq}30CFU/mL$, 10.0% was 30~100 CFU/mL and 5.6% was ${\geq}100CFU/mL$. The detected rate of total coliforms was 6.6%. In conclusion, the ground water quality of 30 military facilities in Seoul, Kyunggi-do, and Inchon-city was acceptable for drinking except for a few detection of total colony count and total coliforms over standard.

• Biomolecules & Therapeutics
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• 제6권1호
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• pp.89-94
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• 1998

CJ50001 is a recombinant human granulocyte colony-stimulating facto, (rHuG-CSF) that stimulates the formation of neutrophils from bone marrow stem cells. It was produced in E. colt and purified through refolding and several processes. We produced CS970125(300) using purified C150001 and additives in order to test the stability of CJ50001. When CS970125(300) was stored at 50'S for more than 1 week, high molecular weight proteins were formed and those proteins were detected by non-reducing SDS-PAGE, gel filtration HPLC, and Western blot. Those proteins showed single band at the same position of CJ50001 in reducing SDS-PAGE. These data indicated that those high molecular weight proteins were the multimers of C150001. In biological assays, iu viro and in viro, the multimers did not have biological activity and inhibitory action to that of CJ 50001. The mutimers did not induce toxicity in mice and rats in acute toxicity test. These results suggest that if Cs970125(300) containing CJ50001 is stored at 5$0^{\circ}C$, CJ50001 will be the multimers that do not have biological activity and inhibitory effect to CJ50001 and do not induce acute toxicity.

• Biomolecules & Therapeutics
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• 제2권3호
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• pp.247-255
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• 1994

As a series of safety studies of DA-3030, a new rhO-CSF, its local irritancy was examined in the rabbits after the following treatment; application into the conjunctival sac of the eye(single), subcutaneous injection(single), intramuscular injection(single), and intravenous injection(8-day repeated). In addition, paravenous irritation of DA-3030 was investigated in mice. The results obtained were as follows. 1. In the result of ocular irritation test, 0.03% solution of DA-3030 could be considered as a non-irritating material. 2. The local irritation of DA-3030 by an injection of 0.5mι of its solution subcutaneously or intramuscularly was negligible and not so much different from that of saline. 3. In the vascular irritancy test, macro- and microscopic observations revealed that the irritating activity of DA-3030 in blood vessels was not different from that of saline when they were injected once a day into vein retroauricularis of rabbits for 8 days.4. The paravenous administration of DA-3030 did not induce any abnormal changes at injection sites except mild swelling in 1 mouse at 3 hours after injection which was thought to be due to slow absorption. The above-mentioned results suggest that DA-3030 has no irritating activity when injected through intravenous or subcutaneous route for clinical practice as 0.03% solution.

• Advances in Computational Design
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• 제1권4호
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• pp.315-327
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• 2016

Damage detection and localisation in structures is essential since it can be a means for preventive maintenance of those structures under service conditions. The use of structural modal data for detecting the damage is one of the most efficient methods. This paper presents comparative performance of various state-of-the-art meta-heuristics for use in structural damage detection based on changes in modal data. The metaheuristics include differential evolution (DE), artificial bee colony algorithm (ABC), real-code ant colony optimisation (ACOR), charged system search (ChSS), league championship algorithm (LCA), simulated annealing (SA), particle swarm optimisation (PSO), evolution strategies (ES), teaching-learning-based optimisation (TLBO), adaptive differential evolution (JADE), evolution strategy with covariance matrix adaptation (CMAES), success-history based adaptive differential evolution (SHADE) and SHADE with linear population size reduction (L-SHADE). Three truss structures are used to pose several test problems for structural damage detection. The meta-heuristics are then used to solve the test problems treated as optimisation problems. Comparative performance is carried out where the statistically best algorithms are identified.

• 대한간호학회지
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• 제43권4호
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• pp.478-485
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• 2013

Purpose: This study was conducted to develop a health education program for preschoolers who have defected from North Korea with their mothers, and to evaluate the effects on health knowledge and behavior. Methods: A non-equivalent control group pre-post test design was used with 58 preschoolers who were assigned to either the experimental or control group (29 for each group). The program was composed of five sessions in health education and contracts. To test the effectiveness of the intervention, health knowledge and behaviors, and total bacterial colony counts on hands were measured at one pretest and two post tests (1 week and 4 weeks after the intervention ended). Data were analyzed using the SAS program. Results: Health knowledge and behavior in the experimental group increased significantly compared to the control group. The effects of the intervention were evident even at 4 weeks after the intervention ended. Total bacterial colony counts in the experimental group decreased significantly at the 1 and 4 week posttest intervention compared to the control group. Conclusion: Results indicate that this program is effective in improving health knowledge and behavior in these children and therefore can be utilized to ensure efficient management their health care.

• 대한치과의사협회지
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• 제58권1호
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• pp.19-26
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• 2019

Purpose: The purpose of this study is to compare the adhesion of Candida albicans according to build angle in 3D printing denture base resin. Methods: The 3D printing was performed by setting the build angle of the disk type specimen designed by CAD program at 0 degree, 30 degrees, 60 degrees, and 90 degrees. Surface roughness was measured using a non-contact 3D microsurface profiler. The specimens were incubated in Candida albicans suspension for 24 hours. The attached Candida albicans were detached by cell scraper. The suspension of detached C. albicans was serially diluted and plated on Trypticase soy broth. After 48 hours of incubation, total colony forming unit was counted. Results: There was no significant difference in surface roughness(Sa) between the test groups, but the interlayer boundary was observed. There was no statistically significant difference in total colony forming units of Candida albicans between the test groups. Conclusion: There was no difference in the average surface roughness and adhesion of Candida albicans between the specimens. It is considered that the setting of the build angle should be set considering the accuracy or strength rather than the roughness of the surface.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2008년도 춘계학술대회 논문집
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• pp.435-438
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• 2008

High temperature deformation of Ti-6Al-4V alloy with a lamellar colony microstructure was investigated by hot compression and torsion tests. The torsion and compression tests were carried out under a wide range of temperatures and strain rates with true strain up to 2 and 0.7, respectively. The processing maps were generated on the basis of compression and torsion test data and using the principles of dynamic materials modeling (DMM). The shapes of the strain-stress curves in alpha-beta region and processing maps obtained on the two different tests have been compared with a view to evaluate the effect of the microstructure evolution on the flow softening behavior of Ti-6Al-4V alloy with a lamellar colony microstructure.

• Toxicological Research
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• 제8권1호
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• pp.29-40
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• 1992

The recombinant glycoprotein, LBD-005 (Lucky R & D Center, Biotechnology)stimulates the growth of stem cells and activates the development of the hematopoietic cell in a similar manner to the naturally occurring GM-CSF. This test was conducted to investigate if LBD-005 had any effect on fertility in male and female rats. Sprague-Dawley rats(88 of each sex) bred at KRICT, were dosed subcutaneously, at a volume of 2ml/kg body weight with LBD-005 at 0, 250, 500 or 1, 000mg/kg body weight. The male rats were dosed, from 6 weeks of asge, for 60 days prior to mating.

• 대한미생물학회지
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• 제21권2호
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• pp.271-276
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• 1986

Amphotericin B and rifampin or 5-fluorocytosine were tested for synergism against Candida albicans in a synthetic mdium. The test was based on viability studies in which fungicidal activity was determined during the incubation hours of drug exposure. Concentration of amphotericin B(0.2ug per ml and 0.4ug per ml) in combination with inactive concentration of rifampin(12.5ug per ml) or 5-fluorocytosine(25ug per ml) resulted in rapid decrease of colony froming unit(CFU). On the basis of these and earlier studies, it is concluded that amphotericin Band rifampin or 5-fluorocytosine are synergistic against various yeasts and yeast-like fungi under widely differing experimental conditions.