• Journal of Animal Science and Technology
• /
• 제57권1호
• /
• pp.4.1-4.5
• /
• 2015

The study was performed to investigate the effect of dietary supplementation of a lipid-encapsulated Zinc oxide on growth parameters and intestinal mucosal morphology piglets born to Duroc-sired Landrace ${\times}$ Yorkshire dams. Twenty-four 30-day-old piglets weaned at 25 days of age were orally challenged with $5{\times}10^8$ colony forming units of enterotoxigenic Escherichia coli (ETEC) K88 and fed one of the four diets for 7 days: (i) a nursery basal diet containing 100-ppm ZnO (referred to as BASAL), (ii) BASAL supplemented with 120-ppm apramycin (referred to as ANTIBIO), (iii) BASAL with 2,400-ppm ZnO (referred to as HIGH), and BASAL containing 100-ppm lipid-encapsulated ZnO (referred to as LE). All piglets were killed at the end of the experiment for histological examination on the intestine. The results showed that the average daily gain (ADG), the villus height: crypt depth (CD) ratio in the ileum, and the goblet cell density of the villus and crypt in the duodenum, jejunum, and colon were greater in the LE-fed group that those of the BASAL (p < 0.05). Fecal consistency score (FCS) and the CD ratio in the ileum were less in the LE-fed group, compared to the BASAL-fed one (p < 0.05). The effects observed in the LE-fed group were almost equal to those of the HIGH-fed group as well as even superior to those of the ANTIBIO-fed group. Taken together, our results imply that dietary supplementation of 100-ppm lipid-encapsulated ZnO is as effective as that of 2,400-ppm ZnO for promoting growth diarrhea and intestinal morphology caused by ETEC infection.

• 한국주조공학회지
• /
• 제10권1호
• /
• pp.57-63
• /
• 1990

Continuous casting of the $Al-CuAl_2$ eutectic composite was carried out by the upward continuous casting process. The morphology of the eutectic growth and the stability of solid-liquid interface were investigated under various growth conditions. It was possible to get the planar solid-liquid interface at the condition of $G_L/R$＝$3.6{\times}10^3^{\circ}Csec/mm^2$. And the colony structures were formed at the conditions of $G_L/R$ < $R=0.33{\times}10^3^{\circ}Csec/mm^2$. The inter-lamellar spacing of $Al-CuAl_2$ eutectic composite was decreased with the increase of pulling speed. The reduction of inter-lamellar spacing & value of $G_L/R$ caused the increase of ultimate tensile strength and Rockwell hardness in $Al-CuAl_2$ eutectic composite.

• Archives of Pharmacal Research
• /
• 제20권5호
• /
• pp.448-453
• /
• 1997

Protoplast fusion between isoleucine-, argihine- and thymidine-requiring auxotroph $(Ile^{-}, Arg^{-}, Thy^{-})$ of Lentinula edodes and arginine-requiring auxotroph $(Arg^-)$ of Coriolus versicolor has been achieved using 30% polyethylene glycol (M.W.4000) in 10 mM $CaCl_2$-glycine solution (pH 8.0). Fusion hybrids were selected in the 0.6 M sucrose supplemented minimal media on the basis of nutritional complementation with fusion frequency of $7.4{\times}10{-6}$ The hybrids included both parental and non-parental types in colony morphology, growth rate and isozyme patterns. We succeeded inter-order protoplast fusion between the auxotrophs of Lentinula edodes and Coriolus versicolor overcoming the natural barriers of incompatibility. We examined the characteristics of the hybrids and clarified the fusion rocess using electron microscopy.

• 한국식물병리학회:학술대회논문집
• /
• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
• /
• pp.151.2-151
• /
• 2003

One fungus was isolated from lesions on imported chinese cabbage leaves in process of quarantine inspection from China. The fungus was identified as Mycosphaerlla brassicicola, based on morphology of perithecia, asci, ascospore, and curtural characteristics. In Korea, this fungus has been quarantine fungus, and not yet report to occur. Perithecia of the fungus were globose, dark brown with apical papilate ostioles. The size was 90-100 ${\times}$ 130-135$\mu\textrm{m}$. Asci were bitunicate, 8-spored and 38-43 ${\times}$ 15-l9$\mu\textrm{m}$. Ascospore were irregularly biseriate, hyaline, cylindrical, 2-celled, and rounded at the ends. Optium growth temperature of the fungus was at 20$^{\circ}C$ on PDA but did rarely grow over 24$^{\circ}C$. Colony on PDA was of black aerial mycelia.

• Journal of Microbiology
• /
• 제44권1호
• /
• pp.1-10
• /
• 2006

Adaptation to any undesirable change in the environment dictates the survivability of many microorganisms, with such changes generating a quick and suitable response, which guides the physiology of bacteria. During nutritional deprivation, bacteria show a stringent response, as characterized by the accumulation of (p)ppGpp, resulting in the repression of stable RNA species, such as rRNA and tRNA, with a concomitant change in colony morphology. However, genes involved in amino acid biosynthesis become over-expressed to help bacteria survive under such conditions. The survivability of pathogenic bacteria inside a host cell also depends upon the stringent response demonstrated. Therefore, an understanding of the physiology of stringent conditions becomes very interesting in regulation of the growth and persistence of such invading pathogens.

• 대한임상검사과학회지
• /
• 제42권2호
• /
• pp.81-85
• /
• 2010

The clinical features of 178 patients with tinea corporis from January to December 2008 in Catholic Skin Clinics were evaluated. KOH examination and culture from the patients lesions were performed. And cultured dermatophytes were identified by colony morphology and microscopic findings. The ratio of male to female with the infection was about 1.3:1 and showed more prevalence in male. The unexposed area (84.5%) was more frequently affected than exposed (15.5%) and the most common site was buttock. Coexisting dermatophytoses in patients with tinea corporis were noted in 27 cases and the most commons were tinea pedis. The isolated dermatophytes were composed of Trichophyton rubrum (91.7%), Microsporum canis (2.6%), T. verrucosum (2.6%), T. mentagrophytes (1.7%), and T. tonsurans (1.7%).

• 약학회지
• /
• 제38권6호
• /
• pp.696-702
• /
• 1994

An interspecific fusant strain, MS1, was obtained by protoplast fusion between S. peucetius subsp. caesius and S. platensis. We studied on the microbiological and cultural characteristics of the fusant MS1. In liquid culture, the viscosity of culture broth of S. peucetius increased during incubation However the fusant MS1 formed pellet like S. platensis without viscosity change. On agar medium the colony morphology of MSI resembled S. platensis but the color was similar to S. peucetius. The fermentation products of the fusant MS1 was identical with S. peucetius.

• 농업과학연구
• /
• 제4권2호
• /
• pp.167-172
• /
• 1977

스코트랜드 National Collection of Industrial Bacteria의 3개 섬유소 분해세균에 대하여 형태적, 생리적, 및 배양적성질을 검토한 결과 NCIB 8077은 Cellulomonas sp.로, NCIB 8633 및 NCIB 8634는 Pseudomonas sp로 각각 분류되었으며 기왕에 보고된 다른 섬유소 분해세균에 대한 분류학상의 문제점을 아울러 고찰하였다.

• Journal of Species Research
• /
• 제7권1호
• /
• pp.9-12
• /
• 2018

In 2016, as part of a larger effort to discover indigenous prokaryotic species in Korea, we isolated the family Deinococcaceae and Planctomycetaceae as unrecorded bacterial species. From the high 16S rRNA gene sequence similarity (>98.5%) and formation of a robust phylogenetic clade with known species, it was determined that each strain was a distinct bacterial species. There are no official reports that these two species have been described in Korea; therefore, the bacterial strains of Deinococcus and Blastopirellula are described for the first time in Korea. Gram reaction, colony and cell morphology, basic biochemical characteristics, and isolation sources are also described in the species description section.

• Restorative Dentistry and Endodontics
• /
• 제11권1호
• /
• pp.97-103
• /
• 1985

The purpose of this study was to compare Black-pigmented Bacteroides isolated from necrotic pulp with the presence or absence of eight symptoms associated with pulpal necrosis and to identify the proportion of each Black-pigmented Bacteroides species. The canal contents of fourteen traumatically and cariously necrotized teeth were sampled with a special technique and cultured aerobically and anaerobically for growth in blood agar plate and for Black-pigmented Bacteroides on selective blood agar plate. Each Black-pigmented Bacteroides species were identified by Gram's stain, hemolysis reaction, colony color and morphology and biochemical tests. The results were as follows; 1. 60.9 percent of the bacteria isolated were anaerobic and 39.1 percent were aerobic. 2. Four Black-pigmented Bacteroides species were isolated; B. loescheii (74.1%), B. denticola (11.1%), B. intermedius (7.4%) and B. gingivalis (7.4%). 3. Black-pigmented Bacteroides was found to be significantly related to sinus tract formation and exudate.