• 한국가금학회지
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• 제7권1호
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• pp.53-64
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• 1980

A total of 1503 specimens were submitted to the Poultry Disease Diagnostic Service Laboratory during the year 1966 and 1978. The most frequently diagnosed diseases in order of prevalence were avian mycoplasmosis, staphylococcosis, colibacillosis, salmonellosis and pullorum disease, the percentages of the conditions being 24.6%, 20.0%, 18.0%, 12.6% and 6.4%, respectively, The drug resistance of pathogenic mirnoorganisms isolated during the year 1978 from chicken with colicabacillosis, staphylococcosis or salmonellosis were investigated by the use of disc diffusion technique, the results being as follow. 1) Drug resistance of 63 strains of Escherichia coli More than 95% of the strains tested were sensitive to colistin and gentamicin. The percentages of strains sensitive to kanamycin, chloramphenicol, ampicillin and nitrofurantoin were 66.7%, 60.3%, 60.3% and 47.6%, respectively. Majority of the strains were highly resistant to streptomycin and tetracyline. All the strains were resisistant to bacitracin lincomycin, oleandomycin, penicillin and erythromycin. All the strains tested were resistant to more than two among 10 drugs in common use such as penicillin, erythromycin, streptomycin, tetracycline, neomycin, chloramphenicol, kanamycin, ampicillin and gentamicin, and 27 different resistance patterns were noted. The most frequent multiple resistance pattern was PC, EM, SM and TC (11.1%). 2) Drug resistance of 48 strains of Salmonella More than 95% of the strains tested were sensitive to colistin, gentamicin ana ampicillin. The percentages of st rains sensitive to kanamycin, tetracycline, neomycin and nitrofurantoin were 81,3%, 79%, 72.9%, and 68.0% respectively. None of them was sensitive to streptomycin, oleandomycin, erythromycin, lincomycin and bacitracin. All the strains were resistant to more than one among 7 drugs in common use such as streptomycin, erythromycin, neomycin, tetracycline, kanamycin, ampicillin and gentamicin. The most frequent resistance pattern was SM and EM(66.7%). 3) Drug resistance of 54 strains of Staphylococci All the strains tested were sensitive to gentmaicin, kanamycin and cephalothin. Majority of them were highly sensitive to bacitracin, methicillin, nitrofurantoin and chloramphenicol. The Percentages of strains sensitive to streptomycin, ampicillin, lincomycin and tetracycline were 66.7%, 55.6%, 44.4% and 27.8%, respectively. Among them, 51 strains were resistant to more than one among 11 drugs in common use such as tetracycline, lincomycin, ampicillin, penicillin, streptomycin, erythromycin, neomycin, oleandomycin, chloramphenicol, methicillin and bacitracin, and thirty one different resistance patterns were noted.

• 한국임상수의학회지
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• 제4권1호
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• pp.409-421
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• 1987

For the epidemiological studies of the respiratory infection from 1,918 heads of adult rabbits from January 1980 to December 1985 at an integrated farm, the causative agents were. Isolated samples 92 from of lungs and nasal discharges, and identified by serological and non-serololgical tests, the loss rates by the infection were also investigatyed, and the antimicrobial sensitivities of the isolates were determined. The results obtained were as fellows : 1. The loss rate by respiratory infection was 11.9% among 1,819 heads which raised and 41.7% among 518 heads which lost by diseases, and the rate increased annually. 2. The loss rate of rabbits in Spring (45.0%), Summer (45.5%) and Winter (53.5%) showed significantly higher (P < 0.01) than that in Autumn(29.1%). 3. The loss rate of Rex (56.3%) showed significantly higher (P<0.05) than New Zealand White (40.7%) and Californian (42.0% ). 4. P. multocida.(37.9%), B. brouchiseptica (16.0%), E. coli (10.6%) and so forth were isolated from 92 samples of lungs and nasal discharges. 5. Sixty fours trains of P. multocida were typed by serological or non-serological tests and their all serotypes were A type (62 strains) except 2 strains of untypable. 6. In antimicrobial sensitivity test for 64 isolates of P. multocida, all the strains tested were highly sensitive to chloramphenicol(95.3%), colistin (95.3%), gentamicin (92.2%), kanamycin (90.6%), neomycin (82.8%) and tetracycline (81.3%), but exhibited low sensitivity to streptomycin (26.6%), and all strains were resistant to lincomycin. 7 Twenty seven isolates of B. brouchiseptica showed higher sensitivity to cephalothin (100%), erythromycin (100% ), gentamicin (100%). trimethoprim+sulfamethoxazole (100%), chloramphenicol(88.9%), kanamycin(88.9%), neomycin (88.9%) and colistin (85.2%), but lower sensitivity to nitrofurantoin (18.5%), penicillin(18.5%). streptomycin(18.5%) and ampicillin (14.8%), and all strains were resistant to lincomycin.

• 대한미생물학회지
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• 제5권1호
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• pp.27-31
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• 1970

Nalidixic acid and six other drugs were studied for in vitro effectiveness against 200 strains of Escherichia coli isolated recently from healthy persons and bactericidal activity of ampicillin against one respective strain of Escherichia coli and Salmonella typhi isolated were also studied. The resutlts obtained by the plate dilution method showed the following percentage of resistance: kanamycin, 2.5%; streptomycin, 12.0%; ampicillin, 13.5%; tetracyclin, 15.5%; chloramphenicol, 17.5%; colistin sulfate, 19.5%. No strains were resistant to nalidixic acid, clearly indicating that nalidixic acid is the most effective drug tested. Ampicillin, measured by test-tube diltion method, was highly bactericidal against Salmonella typhi at the concentration of 2.5mcg/ml and against Escherichia coli at 5mcg/ml.

• 대한수의학회지
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• 제15권1호
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• pp.109-114
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• 1975

A total of 2,065 quarter milk samples of 534 dair cows from 35 herds were examined for mastis and the results obtained were as follows: 1. 654 quarters (31.6%) of 301 dairy cows (56.3%) from 34 herds (97.1%) were infected with mastitis. It was found that 8 cows (1.5%) showed clinical mastitis and other cows were subclinical. 2. Low infection rate of mastis was shown in first delivery cattle and higher infection rate in more deliverable of cattle. 3. The main causative organisms of the matitis were Staphylococcus aureus (19.4%), Streptococcus agalactiae (11.3%) and Streptococcus dysgalatiae (5%). 4. The majority of causative organisms were very resistant to colistin and highly sensitive to tetracycline. Especially Streptococcus strains were highly sensitive to leukomycin, and Staphylococcus aureus were highly sensitive to tetracycline and the other bacteria were highly sensitive to tetracycline and kanamycin.

• 대한수의학회지
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• 제28권1호
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• pp.83-87
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• 1988

The in vitro drug susceptibility of 83 strains of Bordetella bronchiseptica recovered from Korean pigs with atrophic rhinitis was investigated by the use of disk diffusion method. The majority of the organisms were highly resistant in order of prevalence to penicillin(98.7%), ampicillin(91.5%), streptomycin(90.3%), triple sulfa(83.1%), and trimethoprim/sulfamethoxazole(70.7%) while none of them were resistant to gentamicin, only 3.6% to colistin, chloramphenicol and kanamycin and 6.0% to tetracycline. The percentage of the organism resistant to bicozamycin, cephalothin and neomycin were 34.9%, 34.1% and 18.4%, respectively. A high prevalence of multiple drug resistance was observed and the 3 most common resistant patterns among 35 patterns noted were Am Pc Sm Sss Sxt(26.5%), Am Cf Pc Sm Sss Sxt(12.%) and Am Bm Pc Sm Sss Sxt(9.6%) patterns.

• 한국동물위생학회지
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• 제14권1호
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• pp.41-48
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• 1991

Bordetella bronchiseptica were isolated from nasal swabs of the pigs being raised in Eastern Gyeong Nam area from September to December, 1990. Servey on isolation frequency, antimicrobial susceptibility and serum agglutination antibody titer were carried out. The results obtained were summarized as follows : 1. Isolation of B. bronchiseptica were 47 pigs in 113, shown 41.6% of isolation rate and isolation rate were higher in non-vaccination group(60.0%) than vaccination group(28.8%), 2. Isolation rate by ages were the lowest below 1 week ages(22.2%), the highest in 78 weeks ages(55.6 %) and sows was shown 39.3%. 3. It was also found that 47 strains of B. bronchiseptica isolated were highly susceptibility to minocyclin, gentamicin, neomycin, colistin and kanamycin, and highly resistant to penicillin, linsmycin, bacitracin, sulphonamides and chloramphenicol. 4. Isolation rate by the difference of seum agglutination antibody titer were more high at low agglutination titer than high agglutination titer.

• 한국임상수의학회지
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• 제16권1호
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• pp.150-154
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• 1999

This study was designed to examine the therapeutic effect of Italian honeybee (Apis mellifera) venom in piglets with bacterial diarrhea. Preweaning piglets were assigned to treated and nontreated control groups. In treated group, 47 piglets were given natural honeybee venom once a day for 3 consecutive days. The natural honeybees were stung acupoints of GV-1 (Jiao-Chao, at the indentation between the base of tail and the anus) and ST-25 (Hai-men, about 1 cm lateral to the umbilicus). In control group, 44 piglets were intramuscularly injected with a standard dosage of colistin sulfate (300,000 IU/kg of body weight) and antid-iarrheal drug (berberine, 2 ml/kg) once a day for 3 consecutive days. At post-treatment, 90.9% of control piglets and 93.6 % of piglets in treated group recovered from bacterial diarrhea. Bee acupuncture therapy did not show in piglets without any side effects such as allergy, intoxication, hemorrhage, or infection. It might be concluded that honeybee venom therapy was effective in controlling of piglets with bacterial diarrhea.

• 대한수의학회지
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• 제37권4호
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• pp.773-777
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• 1997

A retrospective study of 132 cases of natural Escherichia coli infection in preweaning piglets with diarrhea submitted to the Department of Veterinary Pathology in Seoul National University from 1995 to 1996 was performed to determine the biochemical characteristics and antibiotics susceptibility. Most field isolates were lysine decarboxylase-positive (99.2%), phenylalanine decarboxylase-negative (100%), and fermented sorbitol (91.7%). Antibiotic susceptibilities will be determined by agar diffusion method. A large percentage of isolates were resistant to many antibiotics in common usage. Most isolates were susceptible to colistin (98.8%), gentamycin (64.3%), amikacin (100%), and ceftiour sodium (64.2%), whereas most isolates were notably resistant to ampicillin (86.9%), neomycin (66.7%), streptomycin (84.5%), tetracycline (98.8%), penicillin (98.8%), and amoxacillin (58.3%).

• 미생물학회지
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• 제30권6호
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• pp.438-443
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• 1992

Thirteen strains of Bacillus thuringiensis were isolated from soil in Korea and characterized. The all strains produced parasporal crystals and spores in their cells. Two strains had bipyramidal crystals, seven strains contianed round ones and four strains had unregular ones. Only minor biochemical characteristics of the thirteen isolates were different and distinctive, however general characteristics were similar to the known serotypes of B. thuringiensis. Two strains were resistant to ampicilin. Three strains were resistant to bacitracin, six strains were resistant to cephalothin, two strains were resistant to colistin, HL-68 strain was resistant to gentamycin, HL-67 strain was resistant to kanamycin and HL-71 was resistant to tetracycline. Two strains were resistant to penicillin G. Four strains were toxic to Bombyx mori larvae and eleven strains were toxic to Culex pipiens larvae.

• 한국임상수의학회지
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• 제5권1호
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• pp.53-59
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• 1988

한국 재래산양에 대한 L. monocytogenes균의 감염원을 조사하기 위하여 총 459개의 재래산양 분변, 비즙, 장기, 사료 및 토양재료로부터 균분리를 시도하였으며, 분리균의 항균성 물질에 대한 감수성 및 인공 감염시의 L. monocytogenes의 침입장기를 조사하였던 결과는 다음과 같다. L. monocytogenes는 분변에서만 분리되었으며 (분리율 8.2%) 분변이 본균의 주요 감염원으로 인정되었다. 가검재료로부터 L. monocytogenes의 분리방법으로는 유제화 재료를 혈액배지로써 37에서 24～48시간 동안 호기배양함이 우수하였다. 분리된 L. monocytogenes 8주 중 100%의 균주가 amikacin, colistin, tanamycin, neomycin에 내성을 나타내었으며, clinmycin, gentamicin, lincomycin, methicillin 및 refampin에 87.5%, ampicillin및 oles-ndomycin에 75.0%, Chloramphenico에 62.5%, tetracycline에 50.0% 및 cephalot hin에 37.5%의 균주가 내성이었다. Nitrofurantoin에는 전 균주가 감수성을 나타내었다. L. monocytogenes를 재래산양에 경구 및 정맥내로 접종하였을때 패혈증이 인정되었으며 대부분의 실질장기가 이 균의 침입부위인 것으로 관찰되었다.