• Journal of Microbiology and Biotechnology
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• 제32권12호
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• pp.1599-1604
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• 2022

Storage stability of freeze-dried lactic acid bacteria is a critical factor for their cost-effectiveness. Long-term storage of lactic acid bacteria enables microbial industry to reduce distribution costs. Herein, we investigated the effect of cold adaptation under supercooling conditions at -5℃ on the viability of Leuconostoc mesenteroides WiKim32 during the freeze-drying process and subsequent storage. Cold adaptation increased the thickness of exopolysaccharides (EPS) and improved the viability of freeze-dried Leu. mesenteroides WiKim32. Compared to non-adapted cells, cold-adapted cells showed a 35.4% increase in EPS thickness under supercooling conditions. The viability of EPS-hydrolyzed cells was lower than that of untreated cells, implying that EPS plays a role in protection during the freeze-drying process. Cold adaptation increased the storage stability of freeze-dried Leu. mesenteroides WiKim32. Fifty-six days after storage, the highest viability (71.3%) was achieved with cold adaptation at -5℃. When EPS-containing broth was added prior to the freeze-drying process, the viability further increased to 82.7%. These results imply that cold adaptation by supercooling pretreatment would be a good strategy for the long-term storage of Leu. mesenteroides WiKim32.

• 한국식품조리과학회지
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• 제31권1호
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• pp.62-71
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• 2015

The purpose of this study was to evaluate the changes on the characteristics of the oxidative stability of Tenebrio molitor larvae during cold storage at $4^{\circ}C$. Pretreatment for T. molitor larvae was designed into three methods: raw (R), freeze-dried (F.D.), and pan-fried (P.F.). The water content of the raw sample (61.46%) was higher than those of other samples (F.D.: 5.02%, P.F.: 3.67%) and its high water content was expected to facilitate the oxidation of the raw sample. In our results, the peroxide value and the carbonyl value of all of the samples increased and the raw sample, after storage for 18 day, showed the highest value. The pan-fried sample had no significant increase in its lactic acid content, acid value, and thiobarbituric acid value; whereas those values were increased in the raw sample and the freeze-dried sample (p<0.05). The browning reaction was more progressed in the pan-fried sample than other samples at 0 day, but there was no significant change during the storage. The raw sample and the freeze-dried sample had their browning indexes increase with the increasing storage period (p<0.05). The pan-fried sample produced less oxidation products than the freeze-dried sample, indicating that the unheated sample was more susceptible to oxidation than the heated samples. In conclusion, heating treatment and low water content would be effective for improving the safety and stability of T. molitor larvae during cold storage.

• 대한약침학회지
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• 제6권2호
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• pp.105-111
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• 2003

Objective : The purpose of this study was to investigate the stability of bee venom according to the keeping method and period. Method : The author observed microbial contamination of bee venom in nutrient agar, broth, YPD agar and YPD media and antibacterial activity for S. aureus, E. coli manufactured 12, 6 and 3 months ago as the two type of room temperature and $4^{\circ}C$ cold storage. Results : 1. 1:3,000 and 1:4,000 diluted bee venom solution did not show microbial contamination both room temperature and cold storage within twelve months. 2. There was antibacterial activity of diluted bee venom for S. aureus in cold storage within twelve months and there was no antibacterial activity of diluted bee venom for S. aureus in twelve months, room temperature storage. 3. We could not observe the zone of inhibition around paper disc of all for E.coli. in 1:3,000, 1:30,000 and 1:3,000,000 diluted bee venom solution, respectively. According to results, we expect that diluted bee venom solution is stable both cold and room temperature storage within twelve months.

• 터널과지하공간
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• 제7권1호
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• pp.58-64
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• 1997

A pilot plant of underground cold storage for food has been excavated as a R&D program. For the stability assessment of underground cold storage opeinengs in shallow jointed rocks, three kinds of stability problems were analyzed by numerical methods. For the analysis of unstability by rock block movements, DEM was used considering the statistical distribution of rock joints. Concerning thermally induced cracking, FDM was used with thermomechanical stress analysis. Finally, in order to evaluate the joint failure during the thawing process, BE algorithm was applied. Numerical examples applied for the pilot plant show that the possibility of unstable failure of opeings exists but can be avoided with proper rock reinforcements provided.

• 대한한의학회지
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• 제30권2호
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• pp.127-132
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• 2009

Objectives: This study aimed to investigate the stability of a decoction using three herbal plants and their major components according to the storage conditions and periods. Materials and Methods: A three-herb mixture (1:1:1) of Glycyrrhiza uralensis Fischer, Artemisia capillaris Thunberg, and Poncirus trifoliata Rafinesqui was decocted and kept at room temperature ($25{\pm}2^{\circ}C$) or cold temperature ($4^{\circ}C$) for 0, 2, 4 or 8 weeks in liquid form in a plastic pack under dark conditions. At time points given, they were lyophilized. 200 mg of powdered samples were dissolved in 1 mL of 90% methanol and then applied to a high performance thin layer chromatography (HPTLC) with glycyrrhizin, 6,7-dimethoxycoumarin or poncirin for quantitative or qualitative analysis. Results: There were no gross changes in HPTLC-based compositional band-patterns of the three herbal mixture according to the storage conditions and period. The major components of each herb, glycyrrhizin, 6,7-dimethoxycoumarin and poncirin, showed slight time-dependent reduction in their contents both at room and cold temperature for 8 weeks. Conclusion: We could conclude that the current herbal decoction is generally safe for the stability at both RT or CT for at least 8 weeks. Nevertheless, we proposed that further advanced studies are required for more multiple herbal mixtures and longer storage periods.

• 한국축산식품학회지
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• 제27권3호
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• pp.314-319
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• 2007

본 연구는 도축과정 중 탕침법과 박피법의 탈모기법이 돈육질에 미치는 영향을 분석하고 이를 통해 생산된 식육의 냉장 저장성에 미치는 효과를 규명하고자 실시하였다. 탈모기법에 따른 온도체중과 등지방은 탕박을 실시한 처리구가 박피 처리구보다 높은 수치를 나타내었으며 등심 근의 온도에서도 등지방이 두껍고 도체중이 무거웠던 탕박 거세돈이 가장 높은 수치를 나타내었다. pH와 육색에서는 처리구 간 유의적인 차이를 보이지 않았지만 보수력의 경우는 탕박과 박피 처리에 따른 차이가 뚜렷하게 나타났으며 탕박법에 의해 탈모된 처리구에서 보수력이 저하되는 것으로 분석되었다. 냉장 저장 중 지방산패에 의한 TBA 측정의 변화를 분석한 결과 저장 1일 탕박 거세 돈의 경우가 가장 높게 나타났으나 다른 처리구와 더불어 대부분 신선한 상태를 유지하였다. Metmyoglobin의 경우 저장일 별 처리구 간 유의적인 차이를 보이지 않았으나 TBA측정치의 변화와 유사한 경향을 보였다. 총균수의 경우도 처리구 간 유의적 차이를 보이진 않았으며 저장 7일과 14일 사이에 급속하게 미생물이 증식하는 것으로 관찰되었고 14일 이후에는 완전 부패하였다.

• Preventive Nutrition and Food Science
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• 제9권3호
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• pp.206-212
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• 2004

Prickly pear extract (PPE) was fermented by Lactobacillus rhamnosus LS at 3$0^{\circ}C$ for 2 days. To improve the physicochemical properties of fermented PPE, it was fortified with food polysaccharides (0.2 %) or seaweed calcium before lactic acid fermentation. The viable cell counts, flow behavior, titratable acidity and color stability of fermented PPE were evaluated during 4 weeks of cold storage. Addition of xanthan gum or glucomannan increased the apparent viscosity and acid production, viable cell counts and red color of PPE were also well maintained during the cold storage. However, fermenting PPE with gellan gum resulted in a decrease in relative absorbance, indicating lower color stability. In particular, PPE fortified with carrageenan or alginic acid showed reduced acid production and lower viable cell counts. Addition of seaweed calcium at a 0.1 % level had positive effects on color stability, and helped maintain viable cell counts of 4.1 ${\times}$ 10$^{9}$ CFU/mL. This study demonstrated that xanthan gum could be used as a good thickening agent and stabilizer for retaining viable cell counts and red color during the cold storage in PPE fermented by lactic acid bacteria.

• 디지털융복합연구
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• 제17권2호
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• pp.101-111
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• 2019

냉동 냉장창고의 집적단지인 '콜드체인 클러스터' 구축이 물류업계의 현안으로 급부상하고 있다. 인천항만공사는 한국가스공사와 협력하여 송도 LNG 인수기지에서 발생하는 냉열 에너지를 이용한 냉동 냉장 클러스터를 구축하기 위한 사업을 진행 중에 있다. 본 연구에서는 CFPR 방법론을 활용하여 냉동 냉장 클러스터 활성화 방안을 제시하는 것을 연구의 목적으로 한다. 분석결과, 상위요인에서는 안정성과 수익성 요인이 0.281로 가장 중요시 되는 요인으로 파악되었다. 세부요인으로는 지속가능한 물동량 확보가 가장 높게 나타났고, 임대료 수준, LNG 냉열 활용기술의 지속 가능성과 일반 냉동 냉장창고와의 경쟁, 시설 중복투자 배제 등의 순으로 중요도가 평가 되었다. 향후 연구에서는 활성화 요인을 활용하여 국내 지역별 냉동냉장클러스터를 평가할 필요가 있다.

• 약학회지
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• 제43권6호
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• pp.793-797
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• 1999

Salmonella typhi Ty21a is an attenuated strain of S. typhimurium and used for oral typhoid vaccine. In an attempt to increase the stability of Ty21a manufacturing typhoid vaccine, we studied about the stability of freeze-dried Th21a including additives at various temperature conditions. In order to investigate the freeze-drying rate of Ty21a according to various absorbance, we lyophilized Ty21a by using 8% sucrose as a stabilizer. The optimal freeze-drying rate of Ty21a was appeared when OD (optical density) value of the growth was between 2.5 and 3.0. To investigate the stability of Ty21a at various temperature, the viability was measured after storaging the freeze-dried Ty21a at the room temperature, cold and freezing condition for 1 week. The viability of Ty21a in cold and freezing storage condition was 5 times more stable than in room temperature. To search the most stable additives for the freeze-dried Ty21a, the viability of Ty21a including additives at the various storage condition was estimated. Mannitol and loctose were the most stable additives. Theses results suggest that the OD value of Ty21a growth, low temperature, mannitol and lactose are important factors for the optimal freeze-drying rate, the stable storage and the most stable additives, respectively.

• 한국축산식품학회:학술대회논문집
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• 한국축산식품학회 2005년도 정기총회 및 제35차 춘계 학술 발표대회
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• pp.145-149
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• 2005

This study was carried out to investigate the effect of dietary RVS on the WHC, color stability, and lipid oxidation of Hanwoo (Korean cattle) beef during cold storage after thawing, RVS fed Hanwoo (Korean cattle) beef was more effective in increasing WHC, color stability and delaying lipid oxidation than that without RVS in diet. And 4% RVS fed Hanwoo (Korean cattle) beef showed the greatest effects.