• 제목/요약/키워드: cold-active

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Chinese Influence and Southeast Asian Response: An Interactive Approach (중국의 영향과 동남아의 대응: 상호적 접근시각)

  • Park, Sa-Myung
    • The Southeast Asian review
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    • v.21 no.2
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    • pp.217-261
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    • 2011
  • This study is an attempt to construct a basic framework of analysis about China's political and economic influence on Southeast Asia through traditional Sinocentrism, anti-colonial nationalism, Cold War socialism and post-Cold War capitalism. As to the historical status of Southeast Asia vis-a-vis external forces such as India, China and the West, the colonial discourse tends to put excessive emphasis upon its dependence, and the posy-colonial discourse upon its autonomy. However, this study elucidates the political and economic interactions between China and Southeast Asia in a dynamic perspective, focusing on their reciprocal interactions beyond the essentially static dichotomy of autonomy and dependence. Chinese influence on Southeast asia can be divided into active and reactive one, with the former referring to direct and intended consequences and the latter to indirect and unintended consequences. In the historical process of active and reactive influence, both China and Southeast Asia were fundamentally proactive actors. Thus, the autonomy or dependence of Southeast Asia is just a question of relative one, with its actual extent and degree varying with specific spatial and temporal conditions.

A cold-active acidophilic endoglucanase of Paenibacillus sp. Y2 isolated from soil in an alpine region

  • Lee, Jae Pil;Seo, Gu-Won;An, Shin-Deuk;Kim, Hoon
    • Journal of Applied Biological Chemistry
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    • v.60 no.3
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    • pp.257-263
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    • 2017
  • A cellulolytic strain Y2 was isolated from soil obtained in the Canadian Alpine region. The isolate was identified as Paenibacillus sp. Y2 by 16S rRNA sequencing. When grown in LB medium supplemented with carboxymethyl-cellulose (CMC), CMCase production increased to 122.0% of that observed in LB without CMC. Culture supernatant was concentrated by ultrafiltration and 80% ammonium sulfate precipitates were separated by Hi-Trap Q and CHT-II chromatography. The purified enzyme (EG-PY2) showed a homogeneous single band and the molecular mass was estimated to be 38 kDa by SDS-PAGE. Optimum pH and temperature of the enzyme were 4.5 and $30^{\circ}C$, respectively. The half-life of enzyme activity at 50 was 140.7 min, but the enzyme was drastically inactivated within 5 min at $55^{\circ}C$. The enzyme was highly activated to 135.7 and 126.7% by 5.0 mM of $Cu^{2+}$ or $Mg^{2+}$ ions, respectively, and moderately activated by $Ba^{2+}$ and $Ca^{2+}$ ions, whereas it was inhibited to 76.8% by $Fe^{2+}$, and to ${\leq}50%$ by $Mn^{2+}$, $Co^{2+}$, $Zn^{2+}$, and EDTA. The enzyme was activated to 211.5% in the presence of 0.5 M of NaCl and greatly tolerant to 3.15M of NaCl. The enzyme showed 2.98 times higher ${\beta}$-glucanase activity than CMCase activity. Based on these results, it can be concluded that EG-PY2 is an acidophilic, cold-active, and halotolerant endoglucanase. The authors suggest it is considered to be useful for various industrial applications, such as, fruit juice clarification, acidic deinking processes, high-salt food processing, textile and pulp industries, and for biofuel production from seaweeds.

Cloning, Expression, and Characterization of a Cold-Adapted Shikimate Kinase from the Psychrophilic Bacterium Colwellia psychrerythraea 34H

  • Nugroho, Wahyu Sri Kunto;Kim, Dong-Woo;Han, Jong-Cheol;Hur, Young Baek;Nam, Soo-Wan;Kim, Hak Jun
    • Journal of Microbiology and Biotechnology
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    • v.26 no.12
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    • pp.2087-2097
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    • 2016
  • Most cold-adapted enzymes possess higher $K_m$ and $k_{cat}$ values than those of their mesophilic counterparts to maximize the reaction rate. This characteristic is often ascribed to a high structural flexibility and improved dynamics in the active site. However, this may be less convincing to cold-adapted metabolic enzymes, which work at substrate concentrations near $K_m$. In this respect, cold adaptation of a shikimate kinase (SK) in the shikimate pathway from psychrophilic Colwellia psychrerythraea (CpSK) was characterized by comparing it with a mesophilic Escherichia coli homolog (EcSK). The optimum temperatures for CpSK and EcSK activity were approximately $30^{\circ}C$ and $40^{\circ}C$, respectively. The melting points were $33^{\circ}C$ and $45^{\circ}C$ for CpSK and EcSK, respectively. The ${\Delta}G_{H_2O}$ (denaturation in the absence of denaturing agent) values were 3.94 and 5.74 kcal/mol for CpSK and EcSK, respectively. These results indicated that CpSK was a cold-adapted enzyme. However, contrary to typical kinetic data, CpSK had a lower $K_m$ for its substrate shikimate than most mesophilic SKs, and the $k_{cat}$ was not increased. This observation suggested that CpSK may have evolved to exhibit increased substrate affinity at low intracellular concentrations of shikimate in the cold environment. Sequence analysis and homology modeling also showed that some important salt bridges were lost in CpSK, and higher Arg residues around critical Arg 140 seemed to increase flexibility for catalysis. Taken together, these data demonstrate that CpSK exhibits characteristics of cold adaptation with unusual kinetic parameters, which may provide important insights into the cold adaptation of metabolic enzymes.

Causes of Cold Damage of Rice Plant and Its Control 1. Effects of Temperature on the Growth and Absorption of Mineral Nutrients (수도냉해의 발생기구와 그의 대책에 관한 연구 제1보 수도의 생장과 무기양분흡수에 미치는 온도의 영향)

  • 임형빈
    • Journal of Plant Biology
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    • v.17 no.4
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    • pp.31-37
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    • 1974
  • Seedlings of Nongbaek representing strong cold tolerant variety of rice plant, Jinheung of medium variety and Tongil of weak variety were used respectively for the present study. These seedlings were water cultured in phytotron which maintained at the daytime and night temperature of 30-$25^{\circ}C$, 20-2$0^{\circ}C$, 20-15$^{\circ}C$ and 15-1$0^{\circ}C$. The growth rate of plant height, tillering rate, increase in dry weight and absorption pattern of important mineral nutrients at their early growth stage under each temperature conditions were observed. Generally, it appeared that Nongbaek was more active in the growth of plant height than Jinheung, and Jinheung was more active than Tongil under low temperature condition. The tillering rate of these three varieties was equally rapid while it was decreased in the order of weak cold tolerant variety, such as Tongil, Jinheung and Nongbaek as the temperature declined gradually. The dry weigh tincreasing curve showed almost the same pattern in the varieties at the treatment of each temperature. Under low temperature conditions, Nongbaek showed higher absorption rate of N per dry weight and higher absorption amount of per plant body, Jinheung followed and Tongil was the lowest. The absorption amount of P2O5 increased in the top part compared with the roots as temperature rose and decreased as temperature declined. There seemed to be no difference of absorption among the varieties which have different cold tolerance each other. Under low temperature the absorption rate of K per dry weight was high, as a whole, especially Nongbaek was markedly higher than the other two. The absorption rate of Ca, Mg and Fe was also equally high in all varieties under low temperature and Nongbaek showed a more absorptive tendency in the absorption amount under low temperature.

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Analysis of Nocturnal Cold Air Flow Characteristics for Setting of Tropical Night Response Zone in Daegu (대구시 열대야 대응 구역 설정을 위한 야간 찬공기 유동성 분석)

  • SEO, Bo-Yong;LEE, Sang-Beom;GWON, Soon-Beom;CHA, Jae-Gyu;JUNG, Eung-Ho
    • Journal of the Korean Association of Geographic Information Studies
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    • v.23 no.3
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    • pp.220-235
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    • 2020
  • Heat wave generation in cities is basically affected by global warming, but it is further exacerbated by the impact of artificial heat emission and heat accumulation in the city. In particular, the effects of urban heat waves directly affect the occurrence of tropical nights. Basically, however, the choice of countermeasures against tropical nights is very limited compared to the daytime heat wave response. The purpose of this study was to analyze the characteristics of cold air flow at night as a countermeasure against tropical nights in Daegu Metropolitan City and to suggest its spatial applicability. As a research method, the spatial characteristics (flow velocity, flow rate, flow direction and range) of cold air flow in Daegu were quantitatively analyzed using KLAM_21, a cold air flow analysis program. As a result of the analysis, it was found that cold air generation and flow in the surrounding mountains of Daegu Metropolitan City was very active, but the inflow was limited to the urban area, which has tropical nights. However, it has been shown that the flow of cold air flowing from the surrounding mountains is very active in some urban areas, so it has spatial conditions that are very effective in countering tropical nights. If these spatial conditions are used for the urban planning, it will be very useful to develop countermeasures for tropical nights.

Overturning Resistance of Plain Concrete Piers in OSPG Railroad Bridges

  • Rhee, In-Kyu;Park, Joo-Nam;Choi, Eun-Soo
    • International Journal of Railway
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    • v.3 no.1
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    • pp.1-6
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    • 2010
  • The steel plate-girder bridges with concrete gravity piers have possibilities of overturning by lateral inertial force which can be reproduced by sudden earthquake attack. This paper explores an overturning mechanism of existing concrete gravity pier onto the sandy soil in the event of lateral push-over load by in-situ experimental observation. The in-situ push-over experiment for pier with earth anchors between spread footing and rock beds exhibits a reasonable enhancement of ductility against overturning. In unanchored system, a flexural crack at cold joint of concrete pier is not developed because of the over-turning of the pier. This leads a global instability (rotation) of pier-footing system with relatively low stresses in pier itself. While a lateral load is persistently increased in anchored system, the successive flexural cracking failure at cold joint is observed even after the local shear failure of soil due to redistribution of stress equilibrium between soil and pier structure as long as a tensile action of anchor cable is active.

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Management Strategies of Local Cold Air in Jeongmaek for utilizing urban Ventilation Corridor - A Case Study of the Nak-nam Jeongmaek - (바람길 활용을 위한 정맥의 찬공기 관리 방안 - 낙남정맥을 사례로 -)

  • EUM, Jeong-Hee
    • Journal of the Korean Association of Geographic Information Studies
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    • v.22 no.1
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    • pp.154-167
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    • 2019
  • As urban heat environment problems occur due to climate change, urban thermal environmental problems such as heat waves and tropical nights are becoming more serious in cities. In South Korea, forest areas favorable for generating cold air account for about 63 percent of the land area. Furthermore, the Jeongmaek, the axis of the main mountain ranges of Korea, is located close to the cities. Hence, the management of cold air is an effective way to improve the thermal environment of Korean cities. We selected the Nak-nam Jeongmaek located in the southern part of Korean Peninsular as well as two cities (Jinju-si and Sancheong-gun) located at the Jeongmaek to analyze its cold air characteristics and suggest management strategies of cold air. We used KALM (Kaltluftabflussmodell), a cold air simulation model developed in Germany and identified both the cold air flow and the height of cold air layer generated during 6 hours at night. As a result, the cold air flow generated in the Jeongmaek became clear and the height of cold air layer increased with time. Based on the results, we proposed management plans to maintain and expand the cold air flow. For example, forest areas with active cold air generation were designated as 'cold air conservation areas', and areas requiring management for good cold air flow were as 'cold air management areas'. This study is expected to be useful for establishing systematic urban ventilation plan to improve thermal environment in Korea cities.

Simulation Study on the Application of LNG Cold Energy for Hydrogen Liquefaction Process (수소액화공정에서 LNG 냉열 적용에 관한 시뮬레이션 연구)

  • HAN, DANBEE;BYUN, HYUNSEUNG;BAEK, YOUNGSOON
    • Transactions of the Korean hydrogen and new energy society
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    • v.31 no.1
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    • pp.33-40
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    • 2020
  • As hydrogen utilization becomes more active recently, a large amount of hydrogen should be supplied safely. Among the three supply methods, liquefied hydrogen, which is an optimal method of storage and transportation convenience and high safety, has a low temperature of -253℃, which is complicated by the liquefaction process and consumes a lot of electricity, resulting in high operating costs. In order to reduce the electrical energy required for liquefaction and to raise the efficiency, hydrogen is cooled by using a mixed refrigerant in a precooling step. The electricity required for the precooling process of the mixed refrigerant can be reduced by using the cold energy of LNG. Actually, LNG cold energy is used in refrigeration warehouse and air liquefaction separation process, and a lot of power reduction is achieved. The purpose of this study is to replace the electric power by using LNG cold energy instead of the electric air-cooler to lower the temperature of the hydrogen and refrigerant that are increased due to the compression in the hydrogen liquefaction process. The required energy was obtained by simulating mixed refrigerant (MR) hydrogen liquefaction system with LNG cold heat and electric system. In addition, the power replacement rate of the electric process were obtained with the pressure, the temperature of LNG, the rate of latent heat utilization, and the hydrogen liquefaction capacity, Therefore, optimization of the hydrogen liquefaction system using LNG cold energy was carried out.

Numerical Design of Light-off Auto-Catalyst for Reducing Cold-Start Emissions (냉간시동시 자동차용 저온활성촉매의 성능 향상을 위한 수치적 설계)

  • Jeong, Soo-Jin;Kim, Woo-Seung
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.24 no.9
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    • pp.1264-1276
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    • 2000
  • Light-off catalyst has been used for minimization of cold-start emissions. Improved cold-start performance of light-off catalyst needs the optimal design in terms of flow distribution, geometric surface area, precious metal loading, cell density and space velocity. In this study, these influential factors are numerically investigated using integrated numerical technique by considering not only 3-D fluid flow but also heat and mass transfer with chemical reactions. The present results indicate that uneven catalyst loading of depositing high active catalyst at upstream of monolith is beneficial during warm-up period but its effect is severely deteriorated when the space velocity is above 100,000 $hr^{-1}$ To maximize light-off performance, this study suggests that 1) a light-off catalyst be designed double substrate type; 2) the substrate with high GSA and high PM loading at face be placed at the front monolith; and 3) the cell density of the rear monolith be lower to reduce the pressure drop.

Analysis and Utilization Strategies of Ventilation Corridor Characteristics in Jeon-ju Area (전주지역의 바람길 특성 분석 및 활용 방안)

  • Eum, Jeong-Hee
    • Korean Journal of Environment and Ecology
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    • v.33 no.3
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    • pp.366-374
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    • 2019
  • This study aims to analyze the characteristics of ventilation corridor and propose its utilization strategies in Jeonju city in order to discuss how to utilize urban ventilation corridors as a planning factor for reducing heat wave impact and fine particle pollution. For these purposes, cold air characteristics such as cold air flow and height of cold air in Jeonju area located in the Honam Jeongmaek were analyzed and major ventilation corridors were specified. Based on them, we proposed mountain management strategies for securing and utilizing ventilation corridors. We used KALM (Kaltluftabflussmodell), a cold air simulation model developed in Germany and identified both the cold air flow and the height of cold air layer generated during 6 hours at night. As a result, the cold air flow generated in the forests located in the northeast and east sides of the Jeonju city became clear and the height of cold air layer increased in the valley terrain and farmland areas with time. In particular, Jeonju City has an ideal structure of urban ventilation corridor. Based on the results, the area where the cold air generation is active was designated as the 'cold air conservation area', and the area requiring the management for the good cold air flow was as the 'cold air management area'. This study is expected to be used as basic data of policy making and research for reducing heat wave impact and fine particle pollution such as climate change adaptation policy and urban forest plans for ventilation corridor composition.