• Title/Summary/Keyword: cold stress

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Establishment of Selection Method for Cold-Tolerant Individuals through Evaluating Tolerance of Evergreen Quercus spp. against Cold Stress

  • Park, DongJIn;Yong, Seong Hyeon;Choi, Myung Suk
    • Journal of Forest and Environmental Science
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    • v.35 no.4
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    • pp.232-239
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    • 2019
  • This study was carried out to establish an efficient selection condition for cold-tolerant individuals among evergreen Quercus spp. To select higher cold-tolerant individuals among four species of evergreen Quercus spp. (Q. acuta, Q. glauca, Q. myrsinaefoila, and Q. salicina), an-year-old seedlings of each species were exposed to low temperature in serial, and then examined for the death of cell tissue and the surface temperature was monitored. It was shown that the higher numbers of seedlings of Q. myrsinaefolia were survived than the others when those seedlings are exposed to cold stress. Thus, selection of the cold tolerant individuals was conducted on Q. myrsinaefolia seedlings. The limit low temperature condition for selecting cold-tolerant individuals was -6℃ for 24 hrs because no seedling of Q. myrsinaefolia was survived after exposed to -7℃ for 24 hours. It was shown that the leaf surface temperature of the selected individuals was higher than those of the non-selected individuals when they were exposed to cold-stress while monitoring them using thermal graphic camera. The results in this study can be used for expanding afforestation area of tree species of the warm temperate zone for preparation of climate change.

Comparative Study of Hwangnyeonhaedok-tang and Geongangbuja-tang on the Plasma Hormones Level in Mice Exposed to Cold Stress (황련해독탕(黃連解毒湯)과 건강부자탕(乾薑附子湯)의 Cold Stress로 유발된 생쥐의 혈중(血中) 호르몬 농도변화에 대한 비교연구)

  • Han, Sang-Yong;Kang, Han-Ju;Choi, Eun-Sik;Lee, Ki-Nam;Lee, Tae-Hee;Kim, Yun-Kyung
    • Herbal Formula Science
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    • v.21 no.2
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    • pp.144-157
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    • 2013
  • Objectives : The aim of this study was to evaluate the efficacy of Hwangnyeonhaedok-tang (HH) and Geongangbuja-tang (GB) on the plasma hormone level in mice exposed to cold stress. HH and GB are the representative prescriptions of cold and hot property, respectively. Methods : We established cold condition by confining ICR mice to a $4^{\circ}C$ cage for 24 hours, ICR mice were given a HH (100, 300, 1000 mg/kg) or GB (100, 300, 1000 mg/kg) extract orally twice a day for three consecutive days. From the second day, they were given cold stress ($4^{\circ}C$) for twenty four hours. To measure the plasma corticosterone, insulin, thyroxine, epinephrine and norepinephrine levels of mice, their blood samples were collected from cardiac puncture, immediately centrifuged at $4^{\circ}C$. The protein level of HSP70 and JNK was examined using western blot analysis in cortex and hypothalamus. Results : Oral administration of GB more significantly reduced plasma corticosterone level raised by cold stress than HH. Gardeniae Fructus (CJ), the constituent of HH, significantly increased the thyroxine level. Western blot analysis showed that cold stress-induced Heat shock protein 70 (HSP70) expression was increased by HH and GB, HH decreased JNK expression and GB increased JNK expression dose-depently in hypothalamus. Scutellariae Radix (HG), Zingiberis Rhizoma (GG) and Aconiti Tuber (BJ) decreased HSP70 in hypothalamus and GG, BJ decreased HSP70 in cortex as well. Conclusions : These results suggest Geongangbuja-tang (GB) is more effective for ameliorating the stress response caused by cold stress.

Influence of cold condition exposure on cognitive function and cell proliferation in rats (저온 노출이 인지기능과 뇌신경세포생성에 미치는 영향)

  • Lim, Beak-Vin;Lee, Sung-Pil
    • Science of Emotion and Sensibility
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    • v.14 no.3
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    • pp.425-434
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    • 2011
  • In the present study was to examine the influence of cold stress conditions on memory function in relation with 5-hydroxytryptamine(serotonin, 5-HT), trptophanhydroxylase(TPH) expression and cell proliferation in the hippocampus. For this study, male Sprague-Dawley rats weighing $250{\pm}10g$ (7 weeks in age) were used. The rats were randomly divided into three groups(n = 10 in each group): the $22^{\circ}C$-control group, the $4^{\circ}C$-3 days group, the $4^{\circ}C$-5 weeks group. The environmental temperature at $22^{\circ}C$ set as the normal conditions, $4^{\circ}C$ was as the cold stress conditions. The present results showed that cold stress conditions shorten latency, representing cold stress disturbed memory function. 5-HT and TPH expressions in the dorsal raphe were increased cold stress. Neurogenesis in the dentate gyrus was increased under cold conditions. The present study revealed that cold stress exerted deteriorative memory function. However, through increasing of 5-HT, TPH and BrdU expression under cold stress conditions did not show memory enhancing effect.

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Effects of the Soyosan and Chyengansoyosan on the Regional Brain Monoamines Contents of Cold Swimming Stressed Mice (소요산(逍遙散)과 청간소요산(淸肝逍遙散)이 스트레스 생쥐의 뇌부위별(腦部位別) Monoamines 함량(含量)에 미치는 영향(影響))

  • Lee Jeong-Ho;Chung Dae-Kyoo
    • Journal of Oriental Neuropsychiatry
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    • v.9 no.2
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    • pp.1-18
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    • 1998
  • This study aimed to evaluate the anti-stress effects of soyosan and chyengansoyosan on the contents cold swimming stress.The experimental animals were forced cold swimming stress for 3 minutes, and administered 4㎎/10g of soyosan and chyengansoyosan extract for 7 days before stress. The monoamines contents were measured by HPLC method in various part of mouse brain. The following results were obtained :1. In frontal cortex, the contenrs of norepinephrine ans dopamine increased significantly in the group administered chyrngansoyosan compared with in the group administered non chyengansoyosan and soyosan(control group) of mice stressed by cold swiming.2. In hypothalamus, the content of norepinephrine increased with statistical significance in the group administered soyosan compared with control group of mice stressed by cold swimming. 3. The content of dopamine increased with statistical significance on the group administered chyenfansoyosan and compared with control group of mice stressed by cold swimming in hypothlaamus and hippocampus.4. The content of serotonin increased with statistical significance in the group administered soyosan and compared with control group of mice stressed by cold swimming in hippocampus and corpus striatum.5. In corpus striatum and hippocampus, the content of norepineohrine decreased with statistical significance in the group administered soyosan and chyengandoyosan compared with control group of mice stressed by cold swimming.Judgding from the above results, this sthdy reaches a conclusion that soyosan or chyengansoyosas has significant effects in reducing stress.

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Finite Element Analysis of the Residual Stress by Cold Expansion Method under the Influence of Adjacent Holes (인접 홀의 영향을 받는 홀 확장 잔류응력의 유한요소해석)

  • Kim, Cheol;Yang, Won Ho;Seok, Chang Seong;Kim, Dae Jin
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.31 no.3
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    • pp.79-84
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    • 2003
  • Cold expansion of fastener holes is a mechanical process widely used in the aerospace industry. This treatment leads to an improvement of fatigue behavior due to the developed compressive residual stresses on the hole surface. Despite its importance to aerospace industries, little attention has been devoted to the accurate modeling of the process. This study is devoted to the modeling and simulation of the residual stress resulting from the cold expansion of two adjacent fastener holes. Simultaneous cold expansion of two adjacent holes lead to much higher compressive residual stress than sequential cold expansion.

Effect of cold-spray deposition on deformation of aluminum alloy substrate (초음속 저온분사법에 의한 알루미늄 분말 적층에서 얇은 모재에 발생하는 변형에 대한 연구)

  • Lee Jae-Chul;Chun Doo-Man;Kim Sung-Geun;Ahn Sung-Hoon
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2006.05a
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    • pp.99-100
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    • 2006
  • Cold gas dynamic spray or cold-spray is a deposition process, which causes deformation of a thin substrate. The deformation is usually convex to the deposited side. In this research, the main cause of the deformation was investigated using 6061-T6 aluminum alloy. The effects or anisotropic coefficient or thermal expansion (CTE) or the deposited layer by cold-spray and residual stress were studied by experiments and finite element analysis. The Hole Drilling method was applied to measure residual stress in the cold-spray layer and substrate. The data obtained by the experiments were used for the analysis of substrate deformation. From the result of the analysis, it was concluded that compressive residual stress was the main reason of substrate deformation while CTE had little effect.

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Effect of laser shock peening and cold expansion on fatigue performance of open hole samples

  • Rubio-Gonzalez, Carlos;Gomez-Rosas, G.;Ruiz, R.;Nait, M.;Amrouche, A.
    • Structural Engineering and Mechanics
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    • v.53 no.5
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    • pp.867-880
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    • 2015
  • Mechanical fastening is still one of the main methods used for joining components. Different techniques have been applied to reduce the effect of stress concentration of notches like fastener holes. In this work we evaluate the feasibility of combining laser shock peening (LSP) and cold expansion to improve fatigue crack initiation and propagation of open hole specimens made of 6061-T6 aluminum alloy. LSP is a new and competitive technique for strengthening metals, and like cold expansion, induces a compressive residual stress field that improves fatigue, wear and corrosion resistance. For LSP treatment, a Q-switched Nd:YAG laser with infrared radiation was used. Residual stress distribution as a function of depth was determined by the contour method. Compact tension specimens with a hole at the notch tip were subjected to LSP process and cold expansion and then tested under cyclic loading with R=0.1 generating fatigue cracks on the hole surface. Fatigue crack initiation and growth is analyzed and associated with the residual stress distribution generated by both treatments. It is observed that both methods are complementary; cold expansion increases fatigue crack initiation life, while LSP reduces fatigue crack growth rate.

Dimensional Changes and Residual Stress of Spur Gear According to the Manufacturing Processes -Comparison of Cold Forging Part with Machining Part- (스퍼기어의 제조공정에 따른 치수변화와 잔류응력에 관한 연구 -냉간 단조기어와 기계가공기어 비교-)

  • Kwon, Y.C.;Lee, J.H.;Lee, C.M.;Lee, Y.S.
    • Transactions of Materials Processing
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    • v.16 no.8
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    • pp.575-581
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    • 2007
  • The high dimensional accuracy of the cold forged part could be acquired by the accurate dimensional modification for the die, which is, the dimensional changes from the die through forged part to final part after heat treatment were considered. The experimental and FEM analysis are performed to investigate the dimensional changes from the die to final part on cold forged part, comparing with the machined gear. The dimension of forged part is compared with the die dimension at each stage, such as, machined die, cold forged part, and heat-treated-part. The elastic characteristics and thermal influences on forging stage are analyzed numerically by the $DEFORM-3D^{TM}$. The analyzed residual stress of forged part is considered into the FE-analysis for heat treatment using the $DEFORM-HT^{TM}$. The effects of residual stress affected into the dimensional changes could be investigated by the FEA. Each residual stress of gears was measured practically by laser beam type measurement.

Modeling of Stress-strain Curve for Cold Rolled Electrical Steel (냉간 압연된 전기강판의 응력-변형률 곡선 모델)

  • Yoo, U.K.;Byon, S.M.;Lee, Y.
    • Transactions of Materials Processing
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    • v.17 no.4
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    • pp.272-277
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    • 2008
  • A constitutive equation of the electrical steel strip used for a raw material of transformer is proposed. The stress-strain behavior of electrical steel strip is quite different from that of common carbon steel and/or alloy steel. A series of tensile tests were performed with the specimens made from cold rolled strip. Several thicknesses of the strip were produced by a two-high (with upper and lower rolls) cold rolling pilot mill as reduction ratio increases from 10% to 90%. Its initial thickness of the strip was 2.5mm. Tensile specimens are cut out from the cold rolled strips. Mechanical properties of the steel are examined through rolling direction. Ramberg-Osgood model and the proposed equation are combined to describe the total behavior of stress-strain including instability region. The stress-strain curves calculated from the present constitutive equation are compared with those from experimentally obtained at each test condition of reduction ratios of specimen. Results show that the predicted stress-strain curves are in overall in a good agreement with measured ones.

Vascular dysfunction in patients with type 2 diabetes mellitus

  • Ekta, Khandelwal;Mahaveer Jain;Sumeet Tripathi
    • Annals of Clinical Neurophysiology
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    • v.25 no.1
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    • pp.32-37
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    • 2023
  • Background: Type 2 diabetic mellitus (T2DM) is an emerging global pandemic which is associated with lots of co-morbidities and reported vascular dysfunctions. T2DM associated vascular dysfunctions leads to vasculopathy in the form of altered peripheral vascular dynamics. Cold stress test (CST) is a reliable sympathetic reactivity test used for assessing vascular dysfunctions. In this study we are trying to quantify vascular dysfunctions in T2DM patients non invasively by various parameters of photoplethysmography (PPG) of cold stress test. Methods: Case control study had done in referral health center AIIMS, Raipur. Parameters are recorded by finger-PPG before, during and after CST (1 min) in 2 groups, control (n = 20 healthy volunteers) and case (n = 20 diagnosed T2DM patients). Results: Due to cold stress, PPG parameter peak amplitude was significantly decreased in both healthy and T2DM groups (p <0.001 and p <0.001, respectively). However, recovery trend of amplitude was significantly slow in T2DM compared to healthy subjects. Another PPG parameter peak to peak interval was significantly higher in healthy group compared to T2DM patients. Conclusions: This study showed that T2DM patients has significant deranged pulse volume parameters like amplitude and peak to peak interval can be used to objectively quantify the vasculopathy in T2DM patients by using sympathetic reactivity to cold stress.