• BMB Reports
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• v.40 no.6
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• pp.952-958
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• 2007

We isolated many genes induced from pepper cDNA microarray data following their infection with the soybean pustule pathogen Xanthomonas axonopodis pv. glycines 8ra. A full-length cDNA clone of the Capsicum annuum ankyrin-repeat domain $C_3H_1$ zinc finger protein (CaKR1) was identified in a chili pepper using the expressed sequence tag (EST) database. The deduced amino acid sequence of CaKR1 showed a significant sequence similarity (46%) to the ankyrin-repeat protein in very diverse family of proteins of Arabidopsis. The gene was induced in response to various biotic and abiotic stresses in the pepper leaves, as well as by an incompatible pathogen, such as salicylic acid (SA) and ethephon. CaKR1 expression was highest in the root and flower, and its expression was induced by treatment with agents such as NaCl and methyl viologen, as well as by cold stresses. These results showed that CaKR1 fusion with soluble, modified green fluorescent protein (smGFP) was localized to the cytosol in Arabidopsis protoplasts, suggesting that CaKR1 might be involved in responses to both biotic and abiotic stresses in pepper plants.

• Journal of Korean Medical classics
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• v.24 no.4
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• pp.23-32
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• 2011

The texts of "Hwangjenaegyeong(黃帝內經)" explains Hakjil(瘧疾) in detail, especially in the "Jahak(刺瘧)" chapter, where bloodletting treatment is applied in many cases. The following paper categorized and organized Hakjil(瘧疾) cases treated by bloodletting methods, then analyzed applicable subjects and appropriate time for the procedure based on the texts. Afterwards, the mechanism for the cessation of Hakjil(瘧疾) seizures was examined. The findings of this research are as follows. 1. In the contents of "Hwangjenaegyeong(黃帝內經)", the appropriate time for acupuncture and bloodletting procedure is when Hakjil(瘧疾) seizures start to present themselves. 2. When a seizure takes place as a symptom of the body getting rid of the Hak(瘧) pathogen, Yanggi(陽氣) rushes to the locus of the pathogen, causing congestion of Gi(氣) and Blood(血) resulting in static blood[瘀血]. Therefore, bloodletting at the time of seizure initiation helps the flow of Gi(氣) and Blood(血), preventing the rush of Yanggi(陽氣). This is a restoration of the balancing function of Eum(陰) and Yang(陽), which indicates that bloodletting not only promotes smooth flow of Gi(氣) and Blood(血), but extends its effects to mental functions that balances Eum(陰) and Yang(陽). 3. Although Hakjil(瘧疾) seizures are presented in terms of Gi(氣) and Blood(血) in symptoms such as chill and fever[寒熱], static blood[瘀血], pain, etc., a fundamental disturbance in mental functions that control cold and heat seems to be present.

• Journal of Food Hygiene and Safety
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• v.26 no.4
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• pp.296-301
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• 2011

This study was to evaluate the growth potential of E. coli O157:H7 in lettuce leaf extracts and on lettuce leaf surface at various temperatures. The pathogen can survive and multiply in the extracts and leaf surface of lettuce. The population of E. coli O157:H7 in the lettuce extracts reached to 4.79 log CFU/mL at $37^{\circ}C$. The multiplication of pathogen in lettuce extracts initiated within 10 hours of inoculation over $15^{\circ}C$ conditions. And it can survive in the lettuce leaf extracts at $4^{\circ}C$ for 100 hours at least. And this pathogen can multiply on lettuce leaf surface and the population of pathogen on the lettuce leaf surface increased to 1.82 log CFU/g at $25^{\circ}C$. At $37^{\circ}C$, the pathogen density increased to 1.53 CFU/g within 3 days after inoculation. At all temperature, irrespective of the inoculation level, similar trends in growth of E. coli O157:H7 were observed. These results emphasize the growth potential of E. coli O157:H7 in lettuce leaf extract and on lettuce leaf surface. To reduce the risk of outbreak, it is important to maintain the cold chain system during storage before the consumption.

• Journal of Physiology & Pathology in Korean Medicine
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• v.21 no.4
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• pp.835-841
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• 2007

The followings are concluded from the treatment of headache in Hyungsang medicine, focussed on 11 kinds of headaches in Donguibogam. Headache is classified into overall headache and migraine according to the affected region. The causes are divided into exogenous affection and internal injury; The former brings on headache due to Wind-Cold and headache due to Damp-Heat. The latter, reversal headache, headache due to adverse rising of phlegm, headache due to regurgitation of Gi, headache due to excessive Heat, headache due to excessive Damp, true headache, and alcoholic headache. Headache due to internal injury generally tends to show deficiency syndrome with external affection. Headache due to exogenous affections is common to those who have big head or white skin and to Bangkwang type, and woman. The primary causes are Wind-Cold and Wind-Heat. When the body is observed in the perspective of eight phases, Damp-Heat is to be produced in the front, and Dry-Damp, in the back. Headache due to Damp-Heat is susceptible to Yangmyeong meridian type whose body develops more in the front and to woman. In the perspective of the upper and the lower, Yangdu(that is, head) is related to Eumdu(that is, glans of penis). Headache is also caused by the problems of Eumdu ,such as deficiency of Essence in man, pathologic change of uterus in women, and San syndrome in lower abdomen. In the case of man, headache is frequently severe and difficult to treat because head is a root for man. Disharmony of Gi and blood between the right and the left brings out migraine and headache due to regurgitation of Gi. Migraine is usually accompanied by symptoms of exogenous affection and often afflicts Gi-type, Shin-type, Soyang meridian type, deer type, and Dam-type. Headache due to regurgitation of Gi is brought by Gi deficiency or blood deficiency so that symptoms of exogenous affection do not show. It is mainly common with old people and those who have sunken eyes induced by deficiency of stomach Gi. In the perspective of the upper, the middle, and the lower, the pathologic change of head, chest and abdomen also bring about headache. The pathologic cause of head is Wind-Heat ,which triggers overall headache, migraine, headache due to Wind-Cold, headache due to excessive Heat, The pathogen of chest is phlegm-Fire and brings out headache due to Damp-Heat and headache due to adverse rising of phlegm. The pathologic factor in abdomen is Cold-Damp and produces headache due to adverse rising of phlegm and headache due to excessive Damp. In case of women, headache is generally caused by phlegm-Fire and retention of undigested food.

• The Plant Pathology Journal
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• v.24 no.2
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• pp.118-124
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• 2008

Skin sooty dapple disease, a fungal disease that lowers Asian pear fruit quality, has emerged recently in Korea but has not yet been thoroughly characterized. This disease affects the surface of fruit, leaves, and young shoots of the Asian pear, typically appearing as a dark or pale black dapple on the fruit surface. The disease initiates on the fruit with small circular lesions that become bigger, eventually spreading to form large circular or indefinite lesions. Sparse dark or flourishing white-greyish aerial mycelia and appearance of a dark or pale black dapple on the fruit surface are typical signs of this disease. The disease was severe during cold storage of the Niitaka and Chuhwangbae varieties, but more limited on the Gamcheonbae and Hwangkeumbae varieties. To identify causal pathogens, 123 fungal isolates were obtained from lesions. The fungi that caused typical skin sooty dapple disease symptoms in our bioassay were identified. Based on their morphological characteristics, 74% of the isolates were Cladosporium sp. and 5-7 % of the isolates were Leptosphaerulina sp., Tripospermum sp., or Tilletiopsis sp. None of the isolates caused severe soft rot by injection to a wound plug, but some of the Cladosporium sp. isolates caused mild maceration. Therefore this microbiol complex cannot account for the soft rot also observed in stored fruits. The high frequency of isolation of Cladosporium sp. from disease tissues and bioassay on pear fruit surface suggest that Cladosporium sp. could be a major pathogen in the microbial complex associated with skin sooty dapple disease of the Asian pear in Korea.

• Journal of Korean Medical classics
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• v.22 no.4
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• pp.101-109
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• 2009

The pediatrics has other diagnosis and treatment compared with an adult. It is therefore, the children have different pathogenesis, pathology from adult's. The special feature of the children is two. Firstly, children are the body of the pure Yang[純陽之體], which means the growth and development of them are very fast. Secondly, they have weak muscle, skeletal and digestive function. So we must consider health-preserving method in different physiology and pathology aspects of the children. The children have weak digestive functions, so they should be careful to avoid eating greasy food and overeating. They must eat the nourishing Eum[滋陰] food. The children should wear thin clothes as possible. If the children wear clothes so thick, it promote the Yang (陽), so the Eum(陰) is hurted. The children are to be lack of Eum[陰不足], so they need to grow and raise the Eum(陰) in this way which the lower body is cool. Sleep is important ways to generate Eum Essence[陰精]. So the children should take a bed as change of the season. Children's muscles and skeleton are growing so incomplete that they are hard to handle Wind-Cold pathogen[風寒邪]. Therefore, the children should be training vital Essence and Gi(氣) with appropriate exercise. We should be careful treatment the Hwayeoljeung(火熱證) with purgation[瀉下] - bitter in taste and cold in nature[苦寒藥], aromatic herb[芳香性藥物], sudorific herb[發汗藥] In addition, child has weak digestive function, so doctor should not use a lot of nourishment[滋補].

• Journal of Biosystems Engineering
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• v.43 no.4
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• pp.401-409
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• 2018

Decay of fruit is one of the greatest issues in fruit storage. Purpose: In this study, citrus sterilization was performed to evaluate a dry sterilization method using an atmospheric-pressure nonthermal plasma treatment based on a dielectric-barrier discharge technique. Methods: Citrus samples were stored under four different environmental conditions as follows: group A had cold storage with plasma treatment with a temperature of $6.2{\pm}1.0^{\circ}C$ and relative humidity (RH) of $93.4{\pm}8.2%$, group B had ambient-temperature storage with $22.9{\pm}2.3^{\circ}C$ and $82.1{\pm}4.5%$ RH, group C ambient-temperature storage with plasma treatment with $25.3{\pm}2.2^{\circ}C$ and $90.0{\pm}2.8%$ RH, and group D had cold storage with $5.7{\pm}1.0^{\circ}C$ and $93.4{\pm}6.5%$ RH. Results: As a result of citrus surface sterilization by plasma treatment, treatment groups A and C together showed an average of 16.1 CFU/mL of mold colonies, while control groups B and D showed an average of $2.2{\times}10^2CFU/mL$ or approximately 13 times greater than the treatment groups. Regarding the mean concentration of aerobic bacteria colonies, the treatment groups (A and C) and control groups (B and D) showed an average of 7.1 CFU/mL and $1.9{\times}10^3CFU/mL$, respectively. This is approximately a 270-fold difference in the concentration of pathogen colonies between treatment and control groups. Conclusions: The results showed the potential of nonthermal plasma treatment for citrus storage in enhancing storage duration and quality preservation.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2003.10a
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• pp.24-29
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• 2003

Deep sea water has cold temperature, abundant nutrients and minerals, and good water quality that is pathogen-free and stable. Compared with surface water, deep sea water contains more nutrition salt, such as nitrogen and phosphor. Moreover, if has the good balance of minerals. Because of the ability of the spray drying process to produce a free-flowing power considering of spherical particles with a well-defined size distribution and the rapid drying times for heat-sensitive material, spray drying is attractive for a wide range of applications spray drying is a unique unit operation in which powders are produced from a liquid feed in a single processing step. Key component of the process are atomizer, spray chamber. Design of spray chamber should be based on the atomizer type, the production rate, and the particle size required. Because of the complex processes taking place during spray drying, traditional design method are based on pilot-plant tests and empirical scale-up rules. Modern technique such as CFD have a role to play in design and troubleshooting.

• Proceedings of the Materials Research Society of Korea Conference
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• 2011.05a
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• pp.7.1-7.1
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• 2011

Nanoporous templates have been widely used for the development of new functional nanostructured materials suitable for electronics, optics, magnetism, and energy storage materials. We have prepared nanoporous templates by using thin films of mixtures of polystyrene-block-poly (methyl methacrylate) (PS-b-PMMA) and PMMA homopolymers. These templates have cylindrical nanoholes spanning the entire thickness of the film. Some applications of nanoporous templates are introduced: a) anti-reflective coating, b) the preparation of conducting polymer nanowires of poly (pyrrole), poly (3,4-ethylenedioxy-thiopene) onto a glass coated with indium-tin-oxide, and c) the separation membranes for biomaterials. We found that when the pore fraction of nanoholes in the film was ~0.68, almost zero reflectance at a specific wavelength, which can be changed with film thickness, was achieved at visible wavelengths Furthermore, ultra high density array of conducting nanowires was successfully prepared onto various substrates including flexible polymer. Due to highly alignment of polymer chain along the nanowire direction, the conductivity was much increased. Furthermore, these nanoporous films were found to be very effective for the separation of human Rhinovirus type 14 (HRV 14), major pathogen of a common cold in humans, from the buffer solution. We also found that when the pore size was effectively controlled down to 6 nm, a single file diffusion was observed.

• Proceedings of the Polymer Society of Korea Conference
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• 2006.10a
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• pp.313-313
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• 2006

In this study, we describe a new double layered nanoporous membrane suitable for virus filtration. One layer is an 80 nm thick film having cylindrical pores with diameters of 15 nm and a narrow pore size distribution. This layer is prepared by using a thin film of the mixture of a block copolymer and a homopolymer, and mainly acts to separate viruses. The support layer (${\sim}150\;microns\;thick$) is a conventional micro-filtration membrane with a broad pore size distribution. This asymmetric membrane showed very high selectivity and flux for the separation of human rhinovirus type 14 (HRV 14) which has a diameter of ${\sim}30\;nm$ and is a major pathogen of the common cold in humans.