• Applied Biological Chemistry
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• 제37권6호
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• pp.433-440
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• 1994

상온에서 배양된 녹색조류 Clamydomonas reinhardtii를 $5^{\circ}C$의 저온에 세워두었을 때 세포내에는 괄목할 만한 pyruvate의 축적이 일어났다. 그러나 저온처리된 세포를 다시 상온으로 옮기면 세포내 pyruvate 수준은 감소하기 시작하며 이에 수반하여 superoxide radicals$(O_2^-)$의 발생수준이 증가하였다. 일정기간 후 최고치에 도달한 $O_2^-$ 수준은 다시 비교적 빠르게 떨어지기 시작하였는데, 이때를 전후하여 세포내 superoxide dismutase(SOD)가 현저하게 활성화 되었다. 이러한 결과는 벼의 냉해와 관련하여 얻었던 기존(본 연구실)의 관찰 사실과 매우 유사한 것으로서 고등식물이나 조류가 공히 냉해의 초기과정에 관한한 동일한 기작의 지배를 받고 있음을 시사한다. 아울러 본 연구에서는 저온처리에 의해 야기된 SOD의 활성 증가가 대부분 Mn-SOD의 활성화에 기인하는 것으로 관찰되었다. Mn-SOD는 세포 소기관 중 미토콘드리아에만 존재하므로, 이러한 관찰은 독성이 큰 산소화학종이 과생성되는 부정적 상황 즉 냉해유발 상황에 대처하기 위해 항산소성 효소가 냉해의 초기과정이 진행되는 미토콘드리아에서 유도된다는 개념과 부합된다.

• Applied Biological Chemistry
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• 제30권4호
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• pp.364-370
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• 1987

본연구의 근간이 되는 기본적 가정은, (1) 식물냉해기작에서 2차과정은 반응성이 강한 $O_2^-$의 체내 축적이다. (2) $O_2^-$는 식물체가 저온(低溫)처리를 받고 있는 도중이 아니라 상온(常溫)으로 환원된 뒤에 축적되기 시작한다. (3) $O_2^-$의 축적은 미토콘드리아 막의 상전이가 야기한 세포대사(細胞代謝)$(glycolysis{\rightarrow}TCA\;cycle{\rightarrow}respiratory\;electron\;transport)$의 균형파괴에 기인 한다. 이와 같은 가정을 뒷받침할 수 있는 중요한 연구 결과는 다음과 같다. 먼저 $O_2^-$의 상대적(相對的) 수준(水準)을 측정하는 방법을 확립하였다. 저온처리를 받은 벼 유묘는 처리하지 않은 유묘보다 그 조직 추출액중의 $O_2^-$수준이 높게 나타났다. 48시간의 저온처리중에는 $O_2^-$의 축적이 거의 일어나지 않았으나, 상온(常溫)으로 환원시킨 후 축적되기 시작하여 약 8시간후에 최고치에 도달하였다. Model system을 이용한 연구 결과, 호흡기질을 공급했을 때 미토콘드리아 membrane(SMP)에서 $O_2^-$의 생성이 증가하였다. 효소적으로 발생시킨 $O_2^-$가 존재하는 조건하에서 미토콘드리아막의 전자전달 활성이 저해를 받았다. $O_2^-$의 축적이 최고치에 도달한 후 급격히 감소되는 사실이 관찰되었으며, 이는 Superoxide dismutase(SOD)에 의한 $O_2^-$의 dismutation 결과로 해석되었다.

• 한방안이비인후피부과학회지
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• 제16권3호
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• pp.38-67
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• 2003

The burn is acute skin injury caused by fire, hot water. steam. hot oil, sour and salty. It is occurred frequently in the daily life as well as oriental therapy like moxibustion therapy, physical therapy. Nevertheless, medical treatment of the burn is almost dependent on western cure. So we chose the oriental medicine textbooks and the oriental medicine journals that were dealing with the drugs, processing the drugs. peculiar treatment put first external cure. The results were as follows; 1. The burn is acute skin injury caused by fire, hot water, steam, hot oil, sour and salty. 2. The burn cause blisters, irritability and restlessness, nausea, dryness of mouth, constipation, in case of serious, coma, dyspnea and death. The early stage of the burn, blisters form by skin damage and they burst into skin ulceration from which pus issues, the latter term, the wound form scab and healed up. 3. In a light case, medical treatment of the burn was used external treatment by medicine for externalism use, in a serious case, it was used both as an internal remedy and medicine for outward application. Also in the early stage, it was careful of using the cold and cool medicine, as the process of healing, it was used alleviating pain, detoxicating, moistening the skin, growing muscle and skin, convergence, evacuating pus, regeneration of the tissue, strengthen the spleen and nourishing the stomach. 4. The external treatment medication is Herba Ephedrae Oil(麻油), Radix ET Rhizoma Rhei(大黃), Glauberitum(寒水石), Water(水), Pig OiI(猪油), Pig Fat(猪脂), Radix Angelicae Gigantis(當歸), Rhizoma Coptidis(黃連), Cortex Phellodindri(黃栢). The White of an Egg(鷄子淸), Raw Honey(生蜜), Honey(蜜), Wine(酒), Etc. It is mostly the cold and cool medications. 5. Soft extracted and powered dosage form in external treatment is much used. The soft extracted form(32times used) are mostly Chung Ryang paste(淸凉膏) and Fructus Papaveris paste(罌粟膏). The powered form(30times used) are mostly Bingsang Powder(氷霜散), Bosaenggugo Powder(保生救苦散), Sahwang Powder(四黃散). The others is much a various powder adding solvent. 6. If varicella stage, erosion after varicella stage, oozing stage and extreme pain stage, the powder adding solvent is much used. If little oozing stage. ulcering stage, scabing stage and a chronic stage, Soft extracted dosage form is much used. 7. The most many(26.65%) used method is that apply each medication power mixed water(水), wine(酒), honey(蜜) in a wounded part.

• 화훼연구
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• 제19권2호
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• pp.96-102
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• 2011

'백마'의 휴면개시시기와 타파시기를 구명하기 위해 삽목일 및 입실시기에 따른 생육 및 개화특성을 조사하였다. 삽목시시가 늦어질수록 꽃눈 형성일수와 개화소요 일수가 점차 증가하였다. 9월 18일 채취한 삽수는 100% 개화하였으며, 10월 30일 채취한 삽수는 꽃눈조차 형성되지 않았다. 9월 10일 삽목 후 입실시기에 따른 개화특성을 조사한 결과, '백과'는 입실시기가 늦어짐에 따라 분지발생비율이 점차 증가한 반면, '신마'는 입실시기에 관계없이 약 70-80%의 분지가 발생되었다. 또한 '백마'는 12월 20일 입실시 동해에 의해 1.5%가 고사한 반면, '신마'는 11월 30일부터 고사되기 시작하여 12월 20일 21.7%로 '백마'보다 고사율이 약 5-14배 높은 것으로 나타났다. '백마'의 발뢰일수와 개화소요일수는 입실시기가 늦어짐에 따라 감소되었다. 즉 11월 10일 입실시에는 발뢰일수가 67.9일로 가장 길고, 12월 20일에 입실시에는 50.3일이었으며, '신마'는 12월 10일까지는 입실시기가 늦어짐에 따라 발뢰일수와 개화소요일수가 증가하다가 12월 20일 입실시 감소하였다. 이것으로 보아 '백마'는 9월 말부터 휴면에 돌입하여, 10월 하순경에는 완전히 휴면상태에 이르는 것으로 판단된다.

• The Korean Journal of Physiology and Pharmacology
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• 제13권3호
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• pp.257-263
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• 2009

This study aimed to investigate whether selective serotonin reuptake inhibitors (SSRIs) attenuate brain injury and facilitate recovery following photothrombotic cortical ischemia in mice. Male ICR mice were anesthetized and systemically administered Rose Bengal. Permanent focal ischemia was induced in the medial frontal and somatosensory cortices by irradiating the skull with cold light laser. The animals were treated with fluoxetine or sertraline once a day for 14 d starting 1 h after ischemic insult. Treatment with fluoxetine and sertraline significantly reduced the infarct size. The Evans blue extravasation indices of the fluoxetine- and sertraline-treated groups were significantly lower than that of the vehicle group. Treatment with fluoxetine and sertraline shifted the lower limit of the mean arterial blood pressure for cerebral blood flow autoregulation toward normal, and significantly increased the expression of heme oxygenase-1 (HO-1) and hypoxia-inducible factor-1 ${\alpha}$ (HIF-1 ${\alpha}$) proteins in the ischemic region. These results suggest that SSRIs, such as fluoxetine and sertraline, facilitate recovery following photothrombotic cortical ischemia via enhancement of HO-1 and HIF-1 ${\alpha}$ proteins expression, thereby providing a benefit in therapy of cerebral ischemia.

• 대한한의학회지
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• 제35권1호
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• pp.75-87
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• 2014

Objectives: This study aimed to analyze the utilization of Korean medical clinics by sex. Methods: Data was based on reports about usage and consumption of Korean medicine in 2011 by the Ministry of Health and Welfare and Korea Institute for Health and Social Affairs. 171 Korean medical hospitals and more than 300 Korean medical clinics were selected after 2 rounds of extraction, from the 12,250 Korean medical institutions throughout the country. The investigation was carried out between August 25 and September 30, 2011. The study group was composed of 290 men and 813 women; Korean medical institutions including clinics and hospitals were mainly used for their health care. Results: 1. For sociodemographic distribution, sex, marital status, education level, occupation status, and income level showed statistically significant differences. 2. Females had a lower subjective health status than did males. Muscle injury, hwa-byung, gastric disorder, lumbago, common cold, weight loss, and herbal tonics showed differences in prevalence rates between the sexes. 3. Arthritis, muscle injury, gastric disorder, ankle sprain, herbal tonics, and stroke showed differences in usage rates between the sexes. Likewise, major treatment methods had differences between the sexes. 4. There were no statistically significant differences between the sexes concerning acupuncture. Conclusions: Females were lower than males in several aspects of income and education level and occupation status. Males had a higher subjective health status than did females. Differences by sex of treatment methods and prevalence rates of several diseases existed.

• 사상체질의학회지
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• 제21권3호
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• pp.1-16
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• 2009

1. Objectives: This research was proposed to find out the pathology of Soyangin in Sasang Constitutional Medicine(SCM). 2. Methods: The related contents of the pathology of Soyangin were selected in Je-Ma Lee's literatures such as "Dongmu-YuGo(東武遺稿)"(DYG), "Donguisusebowon-SaSangchobongyun(東醫壽世保元四象草本卷)"(DSS), "Donguisusebowon-GabObon(東醫壽世保元甲午本)"(DGO), "Donguisusebowon-ShinChukbon(東醫壽世保元辛丑本)"(DSC), and the research was written in order to find out the physiology and pathology of Soyangin in SCM. 3. Results and Conclusions: The chronical change of pathologic concept in Soyangin diseases as follows : Pathology in Soyangin diseases was much Hot Qi(熱氣), and more ascending Qi, less descending Qi in DYG, DSS. In "Discourse on the viscera and bowels" of DGO and DSC, Soyangin has a circulation of Water-Food Hot Qi of Spleen Group(脾黨) and Water-Food Cold Qi of Kidney Group(腎黨). Exterior Disease(表病) was the injury of Exterior-Qi such as mouth-hip Qi(口膀胱氣) by Anger-Nature-Qi(怒性氣), and Interior Disease(裏病) was the injury of Interior-Qi such as kidney-large intestine Qi(腎大腸氣) by Sorrow-Emotion-Qi(哀情氣). All diseases of Soyangin are caused by insufficient Cool Yin Qi(陰淸之氣) in Kidney Group(腎黨), so the pathology of Soyangin was focused on Requisite energy(保命之主) and each small viscera and bowels(偏小之臟). In this viewpoint, the schema of Soyangin diseases such as Soyangsangpoong-syndrome(少陽傷風證), Kyulhyung-syndrome(結胸證), Mangeum-syndrome(亡陰證), Hyungkyukyeol-syndrome(胸膈熱證), Sogal-syndrome(消渴證) and Eumhuoyeol-syndrome(陰虛午熱證) were designed to explain the mechanism of each syndrome.

• 대한한의학원전학회지
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• 제28권1호
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• pp.35-53
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• 2015

Objectives : In order to find how reference books of Yi Xue Ru Men reflect the classification and criterion for low back pain(LBP). Methods : From reference books of Yi Xue Ru Men, select the texts on classification and criterion for LBP. Results : According to the causes of LBP, Chao Yuan Fang(巢元方) in Sui Dynasty assorted to 5 types of LBP at the very first. Chen Wu Ze(陳無擇) in Song Dynasty made 7 divisions by external, internal, and non-external, non-internal causes. According to the pulse of LBP, Yan Yong He(嚴用和) first categorized 4 groups, Zhu Zhen Heng(朱震亨) added another 4 groups. Aside from this standard, Zhu(朱震亨) adopted the cause standard. Depending on Yunqi(運氣), Lou Ying(樓英) classified 5 types. But his classification had been not adopted by any TCM books. According to symptom of 6 varieties(六變), Zhang Jie Bin(張介賓) assorted external(表), internal(裏), deficiency(虛), sufficiency(實), cold(寒) and heat(熱), add 2 groups besides them. But his categorization did not reflect Yi Xue Ru Men. Li Chan(李梴), the author of this book chose causes and pulse classification standards that Zhu Zhen Heng had adopt. Conclusions : In the side of classification and criterion for LBP, Li Chan first divided 2 group, external and internal injury. After it he subdivided both groups to 10 subgroup. His classification is similar to Chen(陳無擇)'s, but actually followed the classification for external and internal injury that was invented by Li Dong Yuan(李東垣).

• The Korean Journal of Pain
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• 제25권1호
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• pp.7-15
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• 2012

Background: Neuropathic pain is a chronic pain due to disorder in the peripheral or central nervous system with different pathophysiological mechanisms. Current treatments are not effective. Analgesic drugs combined can reduce pain intensity and side effects. Here, we studied the analgesic effect of nimesulide, nefopam, and morphine with different mechanisms of action alone and in combination with other drugs in chronic constriction injury (CCI) model of neuropathic pain. Methods: Male Wistar rats (n = 8) weighing 150-200 g were divided into 3 different groups: 1- Saline-treated CCI group, 2- Saline-treated sham group, and 3- Drug-treated CCI groups. Nimesulide (1.25, 2.5, and 5 mg/kg), nefopam (10, 20, and 30 mg/kg), and morphine (1, 3, and 5 mg/kg) were injected 30 minutes before surgery and continued daily to day 14 post-ligation. In the combination strategy, a nonanalgesic dose of drugs was used in combination such as nefopam + morphine, nefopam + nimesulide, and nimesulide + morphine. Von Frey filaments for mechanical allodynia and acetone test for cold allodynia were, respectively, used as pain behavioral tests. Experiments were performed on day 0 (before surgery) and days 1, 3, 5, 7,10, and 14 post injury. Results: Nefopam (30 mg/kg) and nimesulide (5 mg/kg) blocked mechanical and thermal allodynia; the analgesic effects of morphine (5 mg/kg) lasted for 7 days. Allodynia was completely inhibited in combination with nonanalgesic doses of nefopam (10 mg/kg), nimesulide (1.25 mg/kg), and morphine (3 mg/kg). Conclusions: It seems that analgesic drugs used in combination, could effectively reduce pain behavior with reduced adverse effects.

• The Korean Journal of Pain
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• 제27권3호
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• pp.246-252
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• 2014

Background: Neuropathic pain is generally defined as a chronic pain state resulting from peripheral and/or central nerve injury. There is a lack of effective treatment for neuropathic pain, which may possibly be related to poor understanding of pathological mechanisms at the molecular level. Curcumin, a therapeutic herbal extract, has shown to be effectively capable of reducing chronic pain induced by peripheral administration of inflammatory agents such as formalin. In this study, we aimed to show the effect of curcumin on pain behavior and serum COX-2 level in a Chronic Constriction Injury (CCI) model of neuropathic pain. Methods: Wistar male rats (150-200 g, n = 8) were divided into three groups: CCI vehicle-treated, sham-operated, and CCI drug-treated group. Curcumin (12.5, 25, 50 mg/kg, IP) was injected 24 h before surgery and continued daily for 7 days post-surgery. Behavioral tests were performed once before and following the days 1, 3, 5, 7 after surgery. The serum COX-2 level was measured on day 7 after the surgery. Results: Curcumin (50 mg/kg) decreased mechanical and cold allodynia (P < 0.001) and produced a decline in serum COX-2 level (P < 0.001). Conclusions: A considerable decline in pain behavior and serum COX-2 levels was seen in rat following administration of curcumin in CCI model of neuropathic pain. High concentration of Curcumin was able to reduce the chronic neuropathic pain induced by CCI model and the serum level of COX-2.