• IMMUNE NETWORK
• /
• 제3권1호
• /
• pp.29-37
• /
• 2003

Background: CC chemokine receptor (CCR) 7 and cognate CCR7 ligands, CCL21 (formerly secondary lymphoid tissue chemokine [SLC]) and CCL19 (formerly Epstein-Barr virus-induced molecule 1 ligand chemokine [ELC]), were known to establish microenvironment for the initiation of immune responses in secondary lymphoid tissue. As described previously, coadministration of DNA vaccine with CCR7 ligand-encoding plasmid DNA elicited enhanced humoral and cellular immunity via increasing the number of dendritic cells (DC) in secondary lymphoid tissue. The author hypothesized here that CCR7 ligand DNA could effectively expand memory CD4+ T cells to protect from viral infection likely via increasing DC number. Methods: To evaluate the effect of CCR7 ligand DNA on the expansion of memory CD4+ T cells, DO11.10.BALB/c transgenic (Tg)-mice, which have highly frequent ovalbumin $(OVA)_{323-339}$ peptide-specific CD4+ T cells, were used. Tg-mice were previously injected with CCR7 ligand DNA, then immunized with $OVA_{323-339}$ peptide plus complete Freund's adjuvant. Subsequently, memory CD4+ T cells in peripheral blood lymphocytes (PBL) were analyzed by FACS analysis for memory phenotype ($CD44^{high}$ and CD62 $L^{low}$) at memory stage. Memory CD4+ T cells recruited into inflammatory site induced with OVA-expressing virus were also analyzed. Finally, the protective efficacy against viral infection was evaluated. Results: CCR7 ligand DNA-treated Tg-mice showed more expanded $CD44^{high}$ memory CD4+ T cells in PBL than control vector-treated animals. The increased number of memory CD4+ T cells recruited into inflammatory site was also observed in CCR7 ligand DNA-treated Tg-mice. Such effectively expanded memory CD4+ T cell population increased the protective immunity against virulent viral infection. Conclusion: These results document that CCR7 and its cognate ligands play an important role in intracellular infection through establishing optimal memory T cell. Moreover, CCR7 ligand could be useful as modulator in DNA vaccination against viral infection as well as cancer.

• 식물병연구
• /
• 제22권1호
• /
• pp.25-31
• /
• 2016

2014년도 충남 당진의 고추포장 바이러스 발생 조사 중, 포장 주변에서 바이러스 병징을 보이는 속속이풀을 수집하였다. 수집한 시료에 대해 여러 고추감염바이러스의 감염여부를 조사한 결과, PMMoV와 CMV에 복합감염되어 있음을 확인하였다. 이는 PMMoV의 잡초 기주로서 속속이풀의 최초 동정이며, 속속이풀의 CMV 감염의 국내 첫 보고이다. 따라서 이들 신규 분리주들의 계통발생학적 위치를 파악하고자, 이들의 전체 게놈 염기서열을 분석하였고 이미 보고된 PMMoV 및 CMV 분리주의 유전정보와 비교 분석하였다. 유전적 측면에서 이미 보고된 고추감염바이러스와의 관계를 알아보고자 속속이풀에서 분리된 PMMoV와 CMV의 전체 염기서열을 분석하였고, 이들의 계통발생학적 특성을 구명하였다. 그 결과, 속속이풀에서 분리한 PMMoV 및 CMV의 분리주가 고추에서 분리한 분리주들과 매우 높은 유사성을 보임을 확인하였고, 이는 속속이풀이 PMMoV 및 CMV의 중간 기주로 작용할 수 있음을 시사한다.

• BMB Reports
• /
• 제37권6호
• /
• pp.709-714
• /
• 2004

The wild-type repressor CI of temperate mycobacteriophage L1 and the temperature-sensitive (ts) repressor CIts391 of a mutant L1 phage, L1cIts391, have been separately overexpressed in E. coli. Both these repressors were observed to specifically bind with the same cognate operator DNA. The operator-binding activity of CIts391 was shown to differ significantly than that of the CI at 32 to $42^{\circ}C$. While 40-95% operator-binding activity was shown to be retained at 35 to $42^{\circ}C$ in CI, more than 75% operator-binding activity was lost in CIts391 at 35 to $38^{\circ}C$, although the latter showed only 10% less binding compared to that of the former at $32^{\circ}C$. The CIts391 showed almost no binding at $42^{\circ}C$. An in vivo study showed that the CI repressor inhibited the growth of a clear plaque former mutant of the L1 phage more strongly than that of the CIts391 repressor at both 32 and $42^{\circ}C$. The half-life of the CIts391-operator complex was found to be about 8 times less than that of the CI-operator complex at $32^{\circ}C$. Interestingly, the repressor-operator complexes preformed at $0^{\circ}C$ have shown varying degrees of resistance to dissociation at the temperatures which inhibit the formation of these complexes are inhibited. The CI repressor, but not that of CIts391, regains most of the DNA-binding activity on cooling to $32^{\circ}C$ after preincubation at 42 to $52^{\circ}C$. All these data suggest that the 131st proline residue at the C-terminal half of CI, which changed to leucine in the CIts391, plays a crucial role in binding the L1 repressor to the cognate operator DNA, although the helix-turn-helix DNA-binding motif of the L1 repressor is located at its N-terminal end.

• Ecology and Resilient Infrastructure
• /
• 제9권2호
• /
• pp.107-112
• /
• 2022

본 연구는 유기물 멀칭재 종류에 따른 토양수분함량과 동자꽃의 생육반응을 비교·분석하여 저관리 옥상녹화에서 멀칭재의 효용성을 알아보고자 수행하였다. 실험구는 멀칭재를 사용하지 않은 대조구 (Cont.; Control)와 코코칩 (C.O; Cocochip), 우드칩 (W.O; Woodchip), 짚거적 (S.T; Straw), 톱밥 (S.A; Sawdust) 등, 총 5개의 처리구로 조성하였다. 실험결과, 유기물 멀칭재 유형에 따른 토양수분함량은 W.O > S.T > Cont. > C.O > S.A 순으로 높게 나타났으며, 특히 톱밥에서 유의적 차이를 보였다. 생육 측정 결과, 초장에서 S.T > Cont. > C.O > W.O > S.A의 순으로 생육이 좋았으며, 초장을 제외한 다른 생육항목에서의 멀칭재별 유의적인 차이는 미미하였다. 상대엽록소함량과 체내수분량은 모두 무처리구보다 우수하게 나타나 유기 멀칭재 처리가 동자꽃의 엽록소함량 및 체내수분량 유지에 효과적인 것으로 판단된다. 특히 실험구 내 토양수분함량은 멀칭재 자체의 성질에 의해 영향을 받는 것으로 나타나 저관리 옥상녹화에서 각 식물의 특성에 적합한 멀칭재 사용이 요구되며 수분 스트레스에 약한 수종 선정 시 유기물 멀칭을 통해 극복할 수 있을 것으로 판단된다.

• Journal of Photoscience
• /
• 제9권2호
• /
• pp.102-105
• /
• 2002

Phoborhodopsin (pR or sensory rhodopsin II, sRII; the absorption maximum of ∼ 500 nm) is a retinoid protein and works as a photoreceptor of the negative phototaxis of Halobacterium salinarum. pharaonis phoborhodopsin (ppR or pharaonis sensory rhodopsin II, psRII) is a corresponding protein of Natronobacterium pharaonis. These sensory proteins form a complex with a cognate transducer protein in the membrane, and this complex transmits the light-signal to the cytoplasm to evoke avoidance reaction from blue-green light. Recently, the functional expression in Escherichia coli membrane of ppR was achieved, which can afford a large amount of the protein and enables mutant studies to clarify the role of various amino acid residues. A truncated transducer which can bind to ppR is also expressed in Escherichia. coli membrane. In this article, we will review properties of ppR mainly using observations of our laboratory; which contains photochemistry (photocycle), light-driven proton uptake, release and transport, F -helix titling during photocycle and association of the transducer.

• Molecules and Cells
• /
• 제27권5호
• /
• pp.503-513
• /
• 2009

Proteoglycans located in basement membranes, the nanostructures underling epithelial and endothelial layers, are unique in several respects. They are usually large, elongated molecules with a collage of domains that share structural and functional homology with numerous extracellular matrix proteins, growth factors and surface receptors. They mainly carry heparan sulfate side chains and these contribute not only to storing and preserving the biological activity of various heparan sulfate-binding cytokines and growth factors, but also in presenting them in a more "active configuration" to their cognate receptors. Abnormal expression or deregulated function of these proteoglycans affect cancer and angiogenesis, and are critical for the evolution of the tumor microenvironment. This review will focus on the functional roles of the major heparan sulfate proteoglycans from basement membrane zones: perlecan, agrin and collagen XVIII, and on their roles in modulating cancer growth and angiogenesis.

• 한국인터넷방송통신학회논문지
• /
• 제10권6호
• /
• pp.169-177
• /
• 2010

철도는 다양한 분야에서 이용되며, 현재 많은 인원과 물류를 운송할 수 있는 효과적이고 친환경적인 교통수단이다. 근래에 들어 지하철을 비롯한 철도에 사고는 인명피해 및 물적 사고로 대부분 인재에 속하며, 이를 방지하기위한 안전 대책을 강구하는 방안이 추진 중이다. 본 논문에서는 철도 건널목 사고 예방을 위한 시스템의 개발을 목적으로 열차의 상황정보를 기반으로 기본적인 철도 건널목 안전관리시스템의 확장뿐만 아니라, 시스템 측면에서도 유연성을 제공할 수 있는 OSGi 프레임워크 기반 상황인지형 스마트 철도 건널목 안전 관리시스템을 구현하는 것을 목적으로 한다.

• 말소리와 음성과학
• /
• 제2권3호
• /
• pp.81-89
• /
• 2010

Korean and three other languages (English, Arabic, and Japanese) were compared with regard to the compensatory movements in a VC (Vowel and Consonant) sequence and word. For this, Korean data were collected from an experiment and the other languages' data from literature. All the test words of the languages had the same syllabic contexture, i.e., /CVCV(r)/, where C was an oral stop and intervocalic consonants were either bilabial or alveolar stops. The present study found that (1) Korean is most striking in the durational variations of segments (vowel and the following hetero-syllabic consonant); (2) unlike the three languages that show a constant sum of VC, Korean yields a three-way distinction in the length of VC according the type (lax unaspirated vs. tense unaspirated vs. tense aspirated) of the following stop consonant; (3) a durational constancy is maintained up to the word level in the three languages, but Korean word duration varies as a function of the feature tenseness of the intervocalic consonants; (4) consonant duration is proven to differentiate Korean the most from the other languages. It is suggested that the durational difference between a lax consonant and its tense cognate(s) and the degree of compensation between V and C are determined by the phonology in each language.

• IMMUNE NETWORK
• /
• 제9권5호
• /
• pp.203-207
• /
• 2009

Background: The FimA of Porphyromonas gingivalis is a crucial pathogenic component of the bacteria and has been implicated as a target for vaccine development against the periodontal diseases. Methods: In this study, the purified fimbriae (FimA subunit polymers) protein was used for immunization in their native form and B hybridoma clones producing antibodies specific to FimA were established. Results: The monoclonal antibodies prepared from selected two clones, designated #123 (IgG2b/ kappa) and #265 (IgG1/kappa), displayed different patterns of binding activity against the cognate antigen. Both antibodies reacted with conformational epitopes expressed by partially dissociated oligomers, but not with monomer as elucidated by Western blot analysis. Ascites fluid containing the monoclonal antibodies showed the inhibitory activity against P. gingivalis to saliva-coated hydroxyapatite beads, an in vitro model for the pellicle-coated tooth surface. Conclusion: These results suggest that the monoclonal antibodies could be used as vaccine material against the periodontal diseases through passive immunization.

• BMB Reports
• /
• 제32권6호
• /
• pp.547-553
• /
• 1999

Aminoacyl-tRNA synthetases are essential enzymes catalyzing the attachment of specific amino acids to cognate tRNAs. In the present work, the substrate analogue L-methionine hydroxamate was used to identify functional residues located in the active site of the E. coli methionyl-tRNA synthetase (MetRS). This compound inhibited bacteria, yeast, and human MetRS activities to a similar degree, suggesting a conserved active site structure and mechanism between MetRSs of different phylogenetic domains. Mutants of the E. coli MetRS resistant to methionine hydroxamate were also isolated. These mutants contained a substitution either at T10, Y15, or Y94. These residues are highly conserved among the different MetRSs and the mutants showed decreased aminoacylation activity, suggesting their functional and structural significances. The putative roles of these residues are discussed on a structural basis.