• Journal of Korean Society of Coastal and Ocean Engineers
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• v.13 no.4
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• pp.309-318
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• 2001

A zero-dimensional simple model is presented to predict the currents in the opening channel of tide embankment and the sea-level response of the interior basin to ocean sea level. In general, a tidal embayment has wide tidal flats so that the effect of tidal flats has been taken into account by formulating the volute variation vs. depth. The model has been verified through the comparison with the 2-dimensional depth-integrated model which can resolve the small area by using the multigrid method. As the results applied to the storage of dredged material of Incheon North Harbour, the results indicate that both models adequately describe the sea-level and current fluctuations in the storage.

• Proceedings of the Korean Geotechical Society Conference
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• 2010.03a
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• pp.1046-1050
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• 2010

During construction of a sea-crossing bridge grouting was used to fill densely the space between the bottom of caisson and the ground. This grout mixture was mixed with an anti-washout admixture after locating accurately the pre-cast caisson on three concrete landing pads. This method differs significantly from the costly conventional method, for bridge foundations offshore, where concrete is placed in situ after excavating inside of a temporary concrete coffering wall. To verify the grouting method in advance, the full-scale field tests were performed twice on land. After identifying the fluidity of the grout material to be filled, finding some possible problems with the main construction and revising the original design, the main construction has been continuing successfully with 20 caissons completed to date. The purpose of this paper is to introduce for the first time in Korea the grouting method including the automatic and the manual monitoring system based on the main construction of the caisson foundation.

• Proceedings of the Korean Geotechical Society Conference
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• 2007.09a
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• pp.665-678
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• 2007

It was applied the grouting method to fill densely the space between the bottom of the caisson and the ground with the grout mixture mixed with anti-washout admixture after locating accurately the precast caisson on three concrete landing pads but it is far different from a costly conventional method, which place concrete to build the foundation of reinforcement concrete on the spot after excavating inside of the temporary coffering wall for the bridge foundation in the sea. To verify the grouting method in advance, the full-scale trial test was performed twice on the land. After confirming the fluidity of material for the injection and some possible problems during construction and then enhancing the original design, the main process is ongoing and it has been finished 12 spots until now. The purpose of this study is to introduces for the first time in Korea the grouting method including the automatic and the manual monitoring process applied to, based on the main process of the caisson foundation finished already in the site. In a similar construction it is sincerely expected to be referred to in the future.

• Journal of Plant Biotechnology
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• v.40 no.4
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• pp.198-202
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• 2013

To produce transgenic cucumber expressing Nit gene coffering abiotic resistance, the cotyledonary-node explants of cucumber (cv. Eunsung) were inoculated with A. tumefaciens transformed with pPZP211 or pCAMBIA2300 carrying Nit gene, that has cis-acting element involved in resistance to various abiotic environmental stresses. After co-cultivation, the procedures of selection, shoot initiation, shoot elongation, and plant regeneration were followed by cotyledonary-node transformation method (CTM, Jang et al. 2011). The putative transgenic plants were selected when shoots were grown to a length greater than 3 cm from the cotyledonary-node explants on selection medium supplemented with 100 mg/L paromomycin as a selectable agent. The confirmation of transgenic cucumber was based on the genomic PCR, Southern blot analysis, RT-PCR, and Northern blot analysis. A 105 shoots (4.12%) selected from the selection mediums were obtained from 2,547 explants inoculated. Of them, putative transgenic plants were only confirmed with 45 plants (1.77%) by genomic PCR analysis. Transgenic plants showed that the Nit genes integrated into each genome of 39 plants (1.53%) by Southern blot analysis, and the expression of gene integrated into cucumber genome was only confirmed at 6 plants (0.24%) by RT-PCR and Northern blot analysis. These results lead us to speculate that the genes were successfully integrated and expressed in each genome of transgenic cucumber.