• Title/Summary/Keyword: coding sequences

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Isolation and sequence analysis of a small cryptic plasmid from Lactobacillus farciminis KCTC3681 (Lactobacillus farciminis로부터 미지의 작은 플라스미드의 분리와 염기서열 분석)

  • Lee, Eun-Mo;Choi, Shin-Geon
    • Journal of Industrial Technology
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    • v.28 no.B
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    • pp.53-57
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    • 2008
  • From the extensive screening for small cryptic plasmid among about 23 lactic acid bacteria (LAB), 2.4 kb of cryptic plasmid was isolated from Lactobacillus farciminis strain KCTC 3681 and named as pLF24. The plasmid pLF24 was a circular molecule of 2,396 base-pairs in length with a G+C content of 38%. Two protein-coding sequences could be predicted. ORF1 and ORF2 showed homologies to plasmids of gram-positive bacteria. The replication protein coded by ORF2 and the plus origin, were similar to replication regions of other gram-positive bacteria as shown in plasmids such as pLH2, pLS141-1 and pLC2. The nucleotide sequence of pLF24 was deposited into Genbank data base with an accession number of EU429343. The newly isolated plasmid can be used for construction of shuttle vector in Lactobacillus bacteria.

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An Effective Block Matching Algorithm for Motion Compensated Coding (이동 보상형 부호화를 위한 효과적인 블록정합 알고리즘)

  • 송현선;김남철;최태호
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.13 no.3
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    • pp.221-230
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    • 1988
  • This paper presents an effective block matching algorithm(BMA) in which the number of search point is about a half of that of three step search, and the number of search step is fixed a four. The performance of the proposed algorithm is compared with those of three step search and one-at-at time search(OTS) for three video sequences composed of 16 framse. Moreover the performance of applying subsampling or integral projection to each BMA for further reducing the amount of computation is considered.

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Introduction to Gene Prediction Using HMM Algorithm

  • Kim, Keon-Kyun;Park, Eun-Sik
    • Journal of the Korean Data and Information Science Society
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    • v.18 no.2
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    • pp.489-506
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    • 2007
  • Gene structure prediction, which is to predict protein coding regions in a given nucleotide sequence, is the most important process in annotating genes and greatly affects gene analysis and genome annotation. As eukaryotic genes have more complicated structures in DNA sequences than those of prokaryotic genes, analysis programs for eukaryotic gene structure prediction have more diverse and more complicated computational models. There are Ab Initio method, Similarity-based method, and Ensemble method for gene prediction method for eukaryotic genes. Each Method use various algorithms. This paper introduce how to predict genes using HMM(Hidden Markov Model) algorithm and present the process of gene prediction with well-known gene prediction programs.

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Variable Sub-pixel Motion Vector Resolution Based on Block Mode Motion Estimation for H.264/AVC

  • Tran, Trung-Kien;Kim, Dae-Yeon;Lee, Yung-Lyul
    • Proceedings of the IEEK Conference
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    • 2008.06a
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    • pp.807-808
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    • 2008
  • In H.264, sub-pel motion estimation (ME) has strong effect when coding video sequences. 1/4-pel performs better at low bitrate while 1/8-pel gives better results at high bitrate. In this paper, a variable sub-pixel motion vector resolution based on block mode motion estimation method is proposed. Experiment results show that the proposed method can take the advantage of 1/4-pel at low bitrate and 1/8-pel at high bitrate. In addition to that, time is reduced from 14% to 53% compared to KTA1.3 with 1/8-pel motion vector (MV) resolution.

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Adaptive coding algorithm using quantizer vector codebook in HDTV (양자화기 벡터 코드북을 이용한 HDTV 영상 적응 부호화)

  • 김익환;최진수;박광춘;박길흠;하영호
    • Journal of the Korean Institute of Telematics and Electronics B
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    • v.31B no.10
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    • pp.130-139
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    • 1994
  • Video compression algorithms are based on removing spatial and/or temproal redundancy inherent in image sequences by predictive(DPCM) encoding, transform encoding, or a combination of predictive and transform encoding. In this paper, each 8$\times$8 DCT coefficient of DFD(displaced frame difference) is adaptively quantized by one of the four quantizers depending on total distortion level, which is determined by characteristics of HVS(human visual system) and buffer status. Therefore, the number of possible quantizer selection vectors(patterns) is 4$^{64}$. If this vectors are coded, toomany bits are required. Thus, the quantizer selection vectors are limited to 2048 for Y and 512 for each U, V by the proposed method using SWAD(sum of weighted absolute difference) for discriminating vectors. The computer simulation results, using the codebook vectors which are made by the proposed method, show that the subjective and objective image quality (PSNR) are goor with the limited bit allocation. (17Mbps)

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Fast Intra Mode Decision using Transform Coefficients (변환 계수를 이용한 고속 인트라모드 결정법)

  • Hwang Gyu-Yong;Park Jong-Bin;Jeon Byeung-Woo
    • 한국정보통신설비학회:학술대회논문집
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    • 2006.08a
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    • pp.255-258
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    • 2006
  • In the H.264/AVC standard, intra prediction increases the coding efficiency of intra macroblocks and by applying rate-distortion optimization to each macroblock, it is possible to choose Inter or Intra encoding adaptively. This sort of encoding scheme, however, increases encoding complexity dramatically and causes troubles in practical applications of the real-time mobile environment. In order to decrease the complexity, variety of methods is proposed but most of those take only its current block into consideration for selecting candidate modes which naturally causes degradation in PSNR (Peak Signal to Noise Ratio). The proposed fast intra mode decision finds the candidate modes by analyzing transformed coefficients of neighboring blocks stored in the buffer at the encoder stage of input sequences. We verify this proposed scheme in complexity, PSNR and bit-rate.

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Comparative Genome Analysis of Sphingomonas chungbukensis DJ77

  • Hai Dang Sy;Kim Young-Pil;Choi Bum-Sun;Um Hyun-Ju;Kim Young-Chang
    • Proceedings of the Microbiological Society of Korea Conference
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    • 2002.10a
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    • pp.175-179
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    • 2002
  • The assemblies of our partial genomic sequence data of Sphingomonas chungbukensis DJ77, with the total size of 877,928 bp, was done by TIGR Assembler. The total size of our current obtained contigs was about 0.73 Mb. A comparative genome analysis between our uncompleted genome and the other completed genomes was performed by taking advantage of the availability of multiple complete genomes in COGs database (Clusters of Orthologous Groups of proteins) to produce the genomic prediction of our S. chungbukensis DJ77. This analysis based on homologues search among completed genomes provides good initial step to our better assigning putative function to predicted coding sequences.

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Complete genome sequence of Streptococcus hyointestinalis B19, a strain producing bacteriocin, isolated from chicken feces

  • Lee, Ju-Eun;Heo, Sunhak;Kim, Geun-Bae
    • Journal of Animal Science and Technology
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    • v.62 no.3
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    • pp.420-422
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    • 2020
  • Streptococcus hyointestinalis B19 was isolated from chicken feces collected from local farm in Anseong, Korea. S. hyointestinalis B19 was shown to produce bacteriocin-like compounds exhibiting inhibitory activities against several pathogens including strains of Clostridium perfringens and Listeria monocytogenes. The whole genome of S. hyointestinalis B19 strain was sequenced using PacBio RS II platform. The genome comprised four contigs with a size of 2,217,061 bp. The DNA G + C content was found to be 42.95 mol%. Annotation results revealed 2,266 coding sequences (CDSs), 18 rRNAs, and 61 tRNA genes. Based on genome analysis, we found that the strain B19 possessed various genes associated with bacteriocin synthesis, modification, and transport.

Adaptive Temporal Rate Control of Video Objects for Scalable Transmission

  • Chang, Hee-Dong;Lim, Young-Kwon;Lee, Myoung-Ho;Ahan, Chieteuk
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • 1997.06a
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    • pp.43-48
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    • 1997
  • The video transmission for real-time viewing over the Internet is a core operation for the multimedia services. However, its realization is very difficult because the Internet has two major problems, namely, very narrow endpoint-bandwidth and the network jitter. We already proposed a scalable video transmission method in [8] which used MPEG-4 video VM(Verification Model) 2.0[3] for very low bit rate coding and an adaptive temporal rate control of video objects to overcome the network jitter problem. In this paper, we present the improved adaptive temporal rate control scheme for the scalable transmission. Experimental results for three test video sequences show that the adaptive temporal rate control can transfer the video bitstream at source frame rate under variable network condition.

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A Cross-Layer Approach to Fair Resource Allocation for Multimedia Service in WiMAX

  • Hong, Seok-Hoon;Lee, Yong-Hun;Jung, Jae-Yoon;Suh, Doug-Young
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.4 no.6
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    • pp.1006-1022
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    • 2010
  • In this paper, a cross-layer resource allocation mechanism is proposed for wireless multimedia service. In particular, a game theory based on quality of service (QoS) for multimedia users is introduced to deal with the fairness of network resource allocation in wireless networks. Moreover, the channel states of wireless users are additionally regarded under the cross-layer design in WiMAX environment. In details, the bargaining solution is adopted to discover the efficient and fair resource allocation strategy for multimedia service in considering QoS in the peak signal-to-noise ratio (PSNR) and the channel states in the carrier-to-interface ratio (CINR). The proposed mechanism is illustrated and evaluated by simulation results of transmitting video sequences in WiMAX environment.