• Asian-Australasian Journal of Animal Sciences
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• 제19권9호
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• pp.1240-1244
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• 2006

The complete coding region sequence of the SRY gene in Chinese swamp buffalo was determined by PCR product sequencing. Comparison of swamp and river buffalo SRY gene sequences revealed a single nucleotide polymorphism (SNP, C/G) at the 202 bp site of the coding region. Further, a total of 124 male domestic buffaloes were genotyped at this SNP site using the PCR-SSCP method, and it was found that all Chinese indigenous swamp buffaloes had a guanine (G) at this site, while introduced river buffaloes and crossbred buffaloes showed a cytosine (C). Our findings suggested that this Y-linked SNP displayed type-specific alleles differentiating swamp and river buffaloes, and could be used as an effective marker to detect crossbreeding of swamp buffaloes with introduced river buffaloes in native buffalo populations, and thereby assess genetic diversity status and make proper conservation decisions for indigenous swamp buffaloes. In addition, this SNP can be potentially applied in the study of Asian water buffalo phylogeny from a male perspective.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2000년도 추계종합학술대회 논문집(4)
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• pp.155-158
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• 2000

In this paper, the quantization noise in block-based video coding is analyzed, and a post-processing method based on the analysis is presented for reducing the quantization noise by using a wavelet transform(WT). In the proposed method, the quantization noise is considered as the sum of a blocking noise expressed as a deterministic profile and the random remainder noise. Each noise is removed in a viewpoint of image restoration using a 1-D WT, which yields a regularized differentiation. The blocking noise first is reduced by weakening the strength of each blocking noise component that appears as an impulse in the first scale wavelet domain. The impulse strength estimation is performed using median filter, quantization parameter(QP), and local activity. The remainder noise, which is considered as a white noise at non-edge pixels, then is reduced by soft-thresholding. The experimental results show that the proposed method yields better performance in terms if subjective quality as well as PSNR performance over VM post-filter in MPEG-4 for all test sequences of various compression ratios. We also present a fast post-processing in spatial domain equivalent to that in wavelet domain for real-time application.

• BMB Reports
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• 제39권3호
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• pp.240-246
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• 2006

The base compositional correlations that hold among various coding and noncoding regions of the canine genome have been analysed. The distribution pattern of genes, on the basis of $GC_3$ composition, shows a wide range similar to that observed in human. However the occurrence of maximum number of genes was observed in the range of 65-75% of $GC_3$ composition. The correlation between the coding DNA sequences of canine with the different noncoding regions (introns and flanking regions) is found to be significant and in many cases the degree of correlation show similarity to human genome. We found that these correlations are not limited to the GC content alone, but is holding at the level of the frequency of individual bases as well. The present study suggests that canines ideally belong to the predicted 'general mammalian pattern' of genome composition along with human beings.

• BMB Reports
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• 제38권6호
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• pp.717-724
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• 2005

The nontoxic B subunit of cholera toxin (CTB) can significantly increase the ability of proteins to induce immunological tolerance after oral administration, when it was conjugated to various proteins. Recombinant CTB offers great potential for treatment of autoimmune disease. Here we firstly investigated the feasibility of silkworm baculovirus expression vector system for the cost-effective production of CTB under the control of a strong polyhedrin promoter. Higher expression was achieved via introducing the partial non-coding and coding sequences (ATAAAT and ATGCCGAAT) of polyhedrin to the 5' end of the native CTB gene, with the maximal accumulation being approximately 54.4 mg/L of hemolymph. The silkworm bioreactor produced this protein vaccine as the glycoslated pentameric form, which retained the GM1-ganglioside binding affinity and the native antigenicity of CTB. Further studies revealed that mixing with silkworm-derived CTB increases the tolerogenic potential of insulin. In the nonconjugated form, an insulin : CTB ratio of 100 : 1 was optimal for the prominent reduction in pancreatic islet inflammation. The data presented here demonstrate that the silkworm bioreactor is an ideal production and delivery system for an oral protein vaccine designed to develop immunological tolerance against autoimmune diabetes and CTB functions as an effective mucosal adjuvant for oral tolerance induction.

• 한국방송∙미디어공학회:학술대회논문집
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• 한국방송공학회 2011년도 하계학술대회
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• pp.192-195
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• 2011

본 논문에서는 JCT-VC(Joint Collaboration Team on Video Coding)에서 표준화가 진행 중인 HEVC(High Efficiency Video Coding)의 Test Model 인 HM1.0과 현재 디지털 방송, 통신, 저장 매체 등 다양한 응용 분야에 사용되고 있는 범용 비디오 코덱들 간의 객관적, 주관적 측면에서의 부호화 성능을 비교한다. 이를 통해 HEVC의 현재 성능 수준에 대한 평가 결과를 보이고, 활용 가능성에 대해 결론을 맺는다. 비교 대상 코덱으로는 H.264/AVC 표준의 S/W 기반인 VideoLAN Project의 x264와 MPEG-2 표준으로 Harmonic사의 H/W기반의 최신형 실시간 인코더인 Electra8000을 사용하였다. 총 5개의 HD(1920x1080)영상에 대한 객관적 성능 비교 결과, HM1.0이 x264 대비 평균 44.93%의 BD-rate 감소와 평균 1.65%의 BD-PSNR 증가를 보였고, Electra8000에 대해서도 월등히 높은 성능차를 보였다. 주관적 화질 비교 결과 동일 PSNR 조건하에서 HM1.0이 범용 비디오 코덱들보다 주관적 화질이 유사하거나 좀 더 나음을 보였다.

• 한국동물위생학회지
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• 제36권1호
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• pp.1-6
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• 2013

Since the 1980's, several kinds of inactivated bovine viral diarrhea virus (BVDV) vaccines have been used to immunize domestic animals such as cattle and goat in Korea. Immunogenicity of the BVDV vaccines has been checked by the Korean Veterinary Authority using laboratory animals. In this study, we applied a molecular method to investigate the genetic characterization of the BVDV genes in six commercial inactivated BVDV vaccines, and determined the efficiency of two extraction reagents (i.e., sodium citrate or isopropyl myristate) to separate the vaccine antigens from the antigen/adjuvant complexes. Six partial non-coding regions (288 bp) were successfully amplified with specific primer sets, which demonstrated that sodium citrate is more efficient in extracting viral RNA from inactivated gel vaccines than isopropyl myristae. In addition, we identified the virus strains from the vaccines by analyzing the nucleotide sequences of the 5' non-coding region (NCR) of BVDV. The nucleotide similarity of the partial 5' NCR ranged from 95.1 to 100% among BVDV vaccine strains, respectively, indicating that a few manufacturers used different BVDV strains to produce their vaccines.

• Parasites, Hosts and Diseases
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• 제54권6호
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• pp.813-817
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• 2016

Armillifer agkistrodontis (Ichthyostraca: Pantastomida) is a parasitic pathogen, only reported in China, which can cause a zoonotic disease, pentastomiasis. A complete mitochondrial (mt) genome was 16,521 bp comprising 13 protein-coding genes (PCGs), 22 tRNA genes, 2 rRNA genes, and 1 non-coding region (NCR). A phylogenetic tree drawn with the concatenated amino acid sequences of the 6 conserved PCGs (atp6, cox1-3, and nad2) showed that A. agkistrodontis and Armillifer armillatus constituted a clade Pentastomida which was a sister group of the Branchiura. The complete mt genome sequence of A. agkistrodontis provides important genetic markers for both phylogenetic and epidemiological studies of pentastomids.

• The Plant Pathology Journal
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• 제16권2호
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• pp.98-104
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• 2000

For the taxonomic evaluaition, 15 strains of the genus Pectobacterium and Erwinia were analyzed for 16S-23S rDNA intergenic spacer regions (ISRs). These species contained two types of ISRs, large and small ISRs. Large ISRs were on the range of 474-569 bp size, and coding transfer $\textrm{RNA}^{11e}$($\textrm{tRNA}^{11e}$) and $\textrm{tRNA}^{Ala}$. Small ISRs were 354-459 bp in length and coding $\textrm{tRNA}^{Glu}$. The sequence variations of two ISRs among species and strains were very high as compared with 16S rRNA gene sequences. By phylogenetic trees on the basis of two ISRs, Pectobacterium ere differentiated into P. carotovorum-P. cactiaidum group and P. chrysanthemi group. However, the taxonomic position of E. cypripedii and E. rhapontici, which were not clear on taxonomic delineation between Pectobacterium and Erwinia, were not clearly resolved on the basis of ISRs.

• 제어로봇시스템학회논문지
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• 제16권10호
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• pp.957-962
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• 2010

If we want to recognize the human's emotion via the facial image, first of all, we need to extract the emotional features from the facial image by using a feature extraction algorithm. And we need to classify the emotional status by using pattern classification method. The AAM (Active Appearance Model) is a well-known method that can represent a non-rigid object, such as face, facial expression. The Bayesian Network is a probability based classifier that can represent the probabilistic relationships between a set of facial features. In this paper, our approach to facial feature extraction lies in the proposed feature extraction method based on combining AAM with FACS (Facial Action Coding System) for automatically modeling and extracting the facial emotional features. To recognize the facial emotion, we use the DBNs (Dynamic Bayesian Networks) for modeling and understanding the temporal phases of facial expressions in image sequences. The result of emotion recognition can be used to rehabilitate based on biofeedback for emotional disabled.

• 한국방송∙미디어공학회:학술대회논문집
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• 한국방송공학회 1996년도 Proceedings International Workshop on New Video Media Technology
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• pp.72-76
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• 1996

We propose a novel algorithm for fractal video sequence coding, based on the circular prediction mapping (CPM), in which each range block is approximated by a domain block in the circularly previous frame. In our approach, the size of the domain block is set to be same as that of the range block for exploiting the high temporal correlation between the adjacent frames, while most other fractal coders use the domain block larger than the range block. Therefore the domain-range mapping in the CPM is similar to the block matching algorithm in the motion compensation techniques, and the advantages of this similarity are discussed. Also we show that the CPM can be combined with non-contractive inter-frame mapping (NCIM), improving the performance of the fractal sequence coder further. The computer simulation results on real image sequences demonstrate that the proposed algorithm provides very promising performance at low bit-rate, ranging from 40 Kbps to 250 Kbps.