• 제목/요약/키워드: coding sequences

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Cloning, Sequencing and Expression Analysis of Porcine Uroplakin II Gene

  • Gwon Deuk-Nam;Kim Jin-Hoe
    • Proceedings of the KSAR Conference
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    • 한국동물번식학회 2002년도 춘계학술발표대회 발표논문초록집
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    • pp.90-90
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    • 2002
  • In this study, we report the cloning of the porcine UPII genomic DNA, which contains a putative full-length open reading frame encoding the UPII protein. A comparison of the porcine UPII gene coding sequence with the previously published mouse UPII sequence demonstrates that only the exon sequences are partially conserved. Northern and immunohistochemical analyses show that the porcine UPII gene is expressed only in the urothelium and that the protein specifically localizes to urothelial superficial cells. (omitted)

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Efficient De-quantization Method based on Quantized Coefficients Distribution for Multi-view Video Coding (다시점 영상 부호화 효율 향상을 위한 양자화 계수 분포 기반의 효율적 역양자화 기법)

  • Park, Seung-Wook;Jeon, Byeong-Moon
    • Journal of Broadcast Engineering
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    • 제11권4호
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    • pp.386-395
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    • 2006
  • Multi-view video coding technology demands the very high efficient coding technologies, because it has to encode a number of video sequences which are achieved from a number of video cameras. For this purpose, multi-view video coding introduces the inter-view prediction scheme between different views, but it shows a limitation of coding performance enhancement by adopting only new prediction method. Accordingly, we are going to achieve the more coding performance by enhancing dequantizer perfermance. Multi-view video coding is implemented basically based on H.264/AVC and uses the same quantization/de-quantization method as H.264/AVC does. The conventional quantizer and dequantizer is designed with the assumption that input residual signal follows the Laplacian PDF. However, it doesn't follow the fixed PDF type always. This mismatch between assumption and real data causes degradation of coding performance. To solve this problem, we propose the efficient de-quantization method based on quantized coefficients distribution at decoder without extra information. The extensive simulation results show that the proposed algorithm produces maximum $1.5\;dB{\sim}0.6\;dB$ at high bitrate compared with that of conventional method.

A H.264 based Selective Fine Granular Scalable Coding Scheme (H.264 기반 선택적인 미세입자 스케일러블 코딩 방법)

  • 박광훈;유원혁;김규헌
    • Journal of KIISE:Computing Practices and Letters
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    • 제10권4호
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    • pp.309-318
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    • 2004
  • This paper proposes the H.264-based selective fine granular scalable (FGS) coding scheme that selectively uses the temporal prediction data in the enhancement layer. The base layer of the proposed scheme is basically coded by the H.264 (MPEG-4 Part 10 AVC) visual coding scheme that is the state-of-art in codig efficiency. The enhancement layer is basically coded by the same bitplane-based algorithm of the MPEG-4 (Part 2) fine granular scalable coding scheme. In this paper, we introduce a new algorithm that uses the temproal prediction mechanism inside the enhancement layer and the effective selection mechanism to decide whether the temporally-predicted data would be sent to the decoder or not. Whenever applying the temporal prediction inside the enhancement layer, the temporal redundancies may be effectively reduced, however the drift problem would be severly occurred especially at the low bitrate transmission, due to the mismatch bewteen the encoder's and decoder's reference frame images. Proposed algorithm selectively uses the temporal-prediction data inside the enhancement layer only in case those data could siginificantly reduce the temporal redundancies, to minimize the drift error and thus to improve the overall coding efficiency. Simulation results, based on several test image sequences, show that the proposed scheme has 1∼3 dB higher coding efficiency than the H.264-based FGS coding scheme, even 3∼5 dB higher coding efficiency than the MPEG-4 FGS international standard.

Complete Mitochondrial Genome Sequence and Genetic Diversity of Duroc Breed (돼지 Duroc 품종에서 미토콘드리아 유전체 서열의 특성과 집단의 유전적 다양성)

  • Cho, 1.C.;Han, S.H.;Choi, Y.L.;Ko, M.S.;Lee, J.G;Lee, J.H;Jeon, J .T
    • Journal of Animal Science and Technology
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    • 제46권6호
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    • pp.937-946
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    • 2004
  • Duroc is widely used to improve the meat quality and productivity. To elucidate the phylogenetic relation and the sequence specificity for the maternal property, the complete sequence of mitochondrial genome was determined and the population diversity of Duroc was investigated in this study. The length of mtDNA tested is 16,584-bp. There are several insertion/deletion mutations in the control region and coding regions for tRNA and rRNA, respectively, but not in peptide-coding regions. Four peptide-coding genes(COⅡ, COⅢ, ND3 and ND4) showed incomplete termination codon sequences such as T--, and two(ND2 and ND4L) did alternative initiation codons(AIC), respectively. Especially, the initiation codon sequences of ND2 gene were polymorphic in this population. Polymorphisms were detected in 11-bp duplication motif within control region as well as ND2 and CYTB. Variation patterns observed from the tests on three mtDNA regions were linked completely and then two haplotypes obtained from combining the data dividing this population. Duroc mtDNA is observed at the European pig cluster in the phylogenetic tree, however, the results from the population analyses supported previous opinions. This study suggests that the breed Duroc was mainly originated from the European pig lineage, and Asian lineage was also used to form the pig breed Duroc as maternal progenitors.

Fast Non-integer Motion Estimation for HEVC Encoder (HEVC 부호화기를 위한 고속 비정수 움직임 추정)

  • Han, Woo-Jin
    • Journal of the Institute of Electronics and Information Engineers
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    • 제51권12호
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    • pp.150-159
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    • 2014
  • The latest video coding standard, HEVC can improve the coding efficiency significantly compared with the H.264/AVC. However the HEVC encoder requires much larger computational complexities. The longer 8-tap interpolation filter of the HEVC which is used in a non-integer motion estimation is one of the reasons and this paper aims to reduce the computational complexities. First of all, three shorter-tap interpolation filters for a motion estimation process are tested rather than the use of a standard interpolation filter. In addition, the fast searching strategies to reduce the number of comparisons for choosing the best non-integer motion vector are proposed. Finally, the interpolation process is selectively applied according to the searching strategy. By combining all of the techniques, the experimental results show that the encoding times can be reduced by 13.6%, 18.5% and 21.1% with the coding efficiency penalties of 0.7%, 1.5% and 2.5%, respectively. For the full-HD video sequences, the coding efficiency penalties are reduced to 0.4%, 1.1% and 1.6% at the same level of the encoding time savings, which shows the effectiveness of the proposed schemes for the high resolution video sequences.

Translational Enhancement by the 5' Leader of Tobacco Mosaic Virus and Soybean Glycinin Gene in Transgenic Tobacco Plants (담배 모자이크 바이러스와 대두 Glycinin 유전자의 5' Leader Sequence를 이용한 외래 유전자의 전이효율 증진)

  • Kang, Hong-Gu;Park, Jee-Won;Kim, Chung-Ho;Lim, Jae-Yun;Choi, Yang-Do
    • Applied Biological Chemistry
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    • 제38권3호
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    • pp.224-231
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    • 1995
  • To increase the expression of a foreign protein in transgenic plant, the benefits of 5'-untranslated leader sequences of tobacco mosaic virus (TMV) RNA or soybean glycinin gene, Gy2, fused to a protein coding sequence were exploited. pGA643-derived plasmid contains 355 promoter of cauliflower mosaic virus, protein coding sequence of maize 10 kDa zein (10kZ) and Gy2 terminator. The leader from Gy2 or TMV RNA was inserted between the promoter and the coding sequence in each construct. The recombinant DNAs were introduced into tobacco plants by Agrobacterium mediated leaf disc transformation method. Although the transgene without the leader had more transcripts than the others, mRNAs containing the leader were translated more efficiently. It might be due to difference in the length of 5'-untranslated sequence and context surrounding the AUG codon, but could be sequence specific rather. These results suggest that the leader sequences of Gy2 and TMV play important roles as an enhancer in translational control of foreign gene in transgenic tobacco plant.

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Transform domain Wyner-Ziv Coding based on the frequency-adaptive channel noise modeling (주파수 적응 채널 잡음 모델링에 기반한 변환영역 Wyner-Ziv 부호화 방법)

  • Kim, Byung-Hee;Ko, Bong-Hyuck;Jeon, Byeung-Woo
    • Journal of Broadcast Engineering
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    • 제14권2호
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    • pp.144-153
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    • 2009
  • Recently, as the necessity of a light-weighted video encoding technique has been rising for applications such as UCC(User Created Contents) or Multiview Video, Distributed Video Coding(DVC) where a decoder, not an encoder, performs the motion estimation/compensation taking most of computational complexity has been vigorously investigated. Wyner-Ziv coding reconstructs an image by eliminating the noise on side information which is decoder-side prediction of original image using channel code. Generally the side information of Wyner-Ziv coding is generated by using frame interpolation between key frames. The channel code such as Turbo code or LDPC code which shows a performance close to the Shannon's limit is employed. The noise model of Wyner-Ziv coding for channel decoding is called Virtual Channel Noise and is generally modeled by Laplacian or Gaussian distribution. In this paper, we propose a Wyner-Ziv coding method based on the frequency-adaptive channel noise modeling in transform domain. The experimental results with various sequences prove that the proposed method makes the channel noise model more accurate compared to the conventional scheme, resulting in improvement of the rate-distortion performance by up to 0.52dB.

Complete chloroplast genome sequences of a major invasive species, Cenchrus longispinus, in Daecheong Island

  • Hyun, Jongyoung;Jung, Joonhyung;NamGung, Ju;Do, Hoang Dang Khoa;Kim, Joo-Hwan
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 한국자원식물학회 2018년도 추계학술대회
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    • pp.64-64
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    • 2018
  • The genus Cenchrus (Poaceae), containing ca. 97 species, is distributed throughout Australia, Africa and Indian sub-continent and which was introduced to the United States and Mexico for use in improved pasture. In Korea, especially Daecheong Island, it is one of the most hazardous invasive plant, which causes serious environmental threats, biodiversity damages and physically negative impact on humans and animals. It can cause serious damage to farms, fields and white sand beaches. However, the chloroplast (cp) genome sequences and information of Cenchrus longispinus have been not addressed, so we provide the complete cp genome of Cenchrus longispinus using next-generation sequencing technology. The size of cp genomes of this Daecheong Island species (Cenchrus longispinus) is 137,144 bp, and it shows a typical quadripartite structure. Consisting of the large single copy (LSC; 80,223 bp), small single copy (SSC; 12,449 bp), separated by a pair of inverted repeats (IRs; 22,236 bp). This cp genome contains 75 unique genes, 4 rRNA coding genes, 33 tRNA coding genes and 21 duplicated in the IR regions, with the gene content and organization are similar to other Poaceae cp genomes. Our comparative analysis identified four cpDNA regions (rpl16, rbcL, ndhH and ndhF) from three Cenchrus species, two Setaria species and one Pennisetum species which may be useful for molecular identification.

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Complete Chloroplast DNA Sequence from a Korean Endemic Genus, Megaleranthis saniculifolia, and Its Evolutionary Implications

  • Kim, Young-Kyu;Park, Chong-wook;Kim, Ki-Joong
    • Molecules and Cells
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    • 제27권3호
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    • pp.365-381
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    • 2009
  • The chloroplast DNA sequences of Megaleranthis saniculifolia, an endemic and monotypic endangered plant species, were completed in this study (GenBank FJ597983). The genome is 159,924 bp in length. It harbors a pair of IR regions consisting of 26,608 bp each. The lengths of the LSC and SSC regions are 88,326 bp and 18,382 bp, respectively. The structural organizations, gene and intron contents, gene orders, AT contents, codon usages, and transcription units of the Megaleranthis chloroplast genome are similar to those of typical land plant cp DNAs. However, the detailed features of Megaleranthis chloroplast genomes are substantially different from that of Ranunculus, which belongs to the same family, the Ranunculaceae. First, the Megaleranthis cp DNA was 4,797 bp longer than that of Ranunculus due to an expanded IR region into the SSC region and duplicated sequence elements in several spacer regions of the Megaleranthis cp genome. Second, the chloroplast genomes of Megaleranthis and Ranunculus evidence 5.6% sequence divergence in the coding regions, 8.9% sequence divergence in the intron regions, and 18.7% sequence divergence in the intergenic spacer regions, respectively. In both the coding and noncoding regions, average nucleotide substitution rates differed markedly, depending on the genome position. Our data strongly implicate the positional effects of the evolutionary modes of chloroplast genes. The genes evidencing higher levels of base substitutions also have higher incidences of indel mutations and low Ka/Ks ratios. A total of 54 simple sequence repeat loci were identified from the Megaleranthis cp genome. The existence of rich cp SSR loci in the Megaleranthis cp genome provides a rare opportunity to study the population genetic structures of this endangered species. Our phylogenetic trees based on the two independent markers, the nuclear ITS and chloroplast MatK sequences, strongly support the inclusion of the Megaleranthis to the Trollius. Therefore, our molecular trees support Ohwi's original treatment of Megaleranthis saniculifolia to Trollius chosenensis Ohwi.

Volumetric Image System for High Efficiency Video Coding (고효율 비디오코딩을 위한 입체영상시스템)

  • Kim, Sang Hyun
    • The Journal of the Korea Contents Association
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    • 제16권1호
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    • pp.515-520
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    • 2016
  • Volumetric image system has many applications recently in education, 3D movie, medical images but these applications have several problems that need to be overcome. Volumetric display may process a amount of visual data and design the high efficient vision system for realtime display. In case of stereo system for volumetric display motion vectors, disparity vectors from the stereoscopic sequences and residual images with the reference images has been transmitted, and the stereoscopic sequences have been reconstructed at the receiver for volumetric display. So central issue for the design of efficient volumetric image system lies in selecting an appropriate stereo matching and robust vision system. In this paper, we proposed high efficient vision system, which design vision stage with rotating and moving horizontally, and match the successive stereo image efficiently. In experimental results with volumetric image system, the proposed method represents high efficiency with minimizing error and low computational load for volumetric display.