• Journal of Microbiology and Biotechnology
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• v.26 no.8
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• pp.1473-1480
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• 2016

This study focused on the variations in the non-coding sequences between ctxB and rstR of various CTX phages. The non-coding sequences of CTX-1 and CTX-cla are phage type-specific. The length of the non-coding region of CTX-1 and CTX-cla is 601 and 730 nucleotides, respectively. The non-coding sequence of CTX phage could be divided into three regions. There is a phage type-specific Variable region between two homologous Common regions (Common regions 1 and 2). The non-coding sequence of RS1 element is similar to CTX-1 except that Common region 1 is replaced by a short RS1-specific sequence. The non-coding sequences of CTX-2 and CTX-cla are homologous, indicating the non-coding sequence of CTX-2 is derived from CTX-cla. The non-coding region of CTX-O139 is similar to CTX-cla and CTX-2; however, it contains an extra phage type-specific sequence between Common region 2 and rstR. The variations in the non-coding sequences of CTX phages might be associated with the difference in the replication efficiency and the directionality in the integration into the V. cholerae chromosome.

• Toxicological Research
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• v.17
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• pp.157-166
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• 2001

Almost all currently available retroviral vectors based on murine leukemia virus (MLV) contain one or more viral coding sequences. Because these sequences are also present in the packaging genome, it has been suggested that homologous recombination may occur between the same nucleotide sequence in the packaging genome and the vector, resulting in the production of replication competent retrovirus (RCR). Up until now, it has been difficult to completely remove viral coding sequences since some were thought to be involved in the optimum function of the retroviral vector. For example, the gag coding sequence present in almost all available retroviral vectors has been believed to be necessary for efficient viral packaging, while the pol coding sequence present in the highly efficient vector MFG has been thought to be involved in achieving the high levels of gene expression. However, we have now developed a series of retroviral vectors that are absent of any retroviral coding sequences but produce even higher levels of gene expression without compromising viral titer. In these vectors, the intron and exon sequences from heterologous cellular or viral genes are present. When compared to the well known MLV-based vectors, some of these newly developed vectors have been shown to produce significantly higher levels of gene expression for a longer period. In an experimental system that can maximize the production of RCR, our newly constructed vectors produced an absence of RCR. These vectors should prove to be safer than other currently available retroviral vectors containing one or more viral coding sequences.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2000.10a
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• pp.31-50
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• 2000

Almost all currently available retroviral vectors based on murine leukemia virus (MLV) contain one or more viral coding sequences Because these sequences are also present in the packaging genome, it has been suggested that homologous recombination may occur between the same nucleotide sequence in the packaging genome and the vector, resulting in the production of replication competent retrovirus (RCR). Up until now, it has been difficult to completely remove viral coding sequences since some were thought to be involved in the optimum function of the retroviral vector. For example, the gag coding sequence present in almost all available retroviral vectors has been believed to be necessary for efficient viral packaging, while the pol coding sequence present in the highly efficient vector MFG has been thought to be involved in achieving the high levels of gene e(pression. However, we have now developed a series of reroviral vectors that are absent of any retroviral coding sequences but produce even higher levels of gene expression without compromising viral titer. In these vectors the intron and exon sequences from heterologous cellular or viral genes are present, When compared to the well blown MLV-based vectors, some of these newly developed vectors have been shown to produce significantly higher levels of gene expression for a longer period. In an experimental system that can maximize the production of RCR, our newly constructed vectors produced an absence of RCR. These vectors should prove to be safer than other currently available retroviral vectors containing one or more viral coding sequences

• Journal of Broadcast Engineering
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• v.13 no.3
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• pp.289-298
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• 2008

We propose an SNR-scalable coding algorithm for three-dimensional mesh sequences based on singular value decomposition (SVD). SVD achieves a coding gain by representing a mesh sequence with a small number of basis vectors and singular values. First, we introduce a bit plane coding scheme and derive a quantitative relationship between each bit plane and the reconstructed image quality. Using the relationship, we develop a rate-distortion (RD) optimized coding algorithm. Moreover, we propose prediction techniques to exploit the spatio-temporal correlations in real mesh sequences. Simulation results demonstrate that the proposed algorithm provides significantly better RD performance than conventional SVD coders.

• Proceedings of the IEEK Conference
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• 2002.07a
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• pp.706-709
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• 2002

This paper describes an effective technique for coding video sequences based on JPEG2000 codec. In the proposed method, multiple frames are combined into one large picture by interleaving each pixel data. A large picture enables images to be coded more efficiently and image quality is improved. A video sequence is efficiently coded by adapting the time correlation of the video sequences to spatial correlation. We demonstrated the effectiveness of this method by encoding video sequences using JPEG2000.

• Proceedings of the IEEK Conference
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• 2002.07b
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• pp.908-911
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• 2002

Over the past 30 years, many efficient 2D video coding techniques have been presented and developed from many research centers for commercialization. However, direct application of these monocular compression schemes is not optimal for stereo video coding. In this paper, we present a new technique for coding stereo video sequences based on Discrete Wavelet Transform (DWT). The proposed technique exploits Zerotree Entropy Coding (ZTE) that makes use of the wavelet block concept to achieve low bit rate stereo video coding. The one of two image streams, called main stream, is independently coded by modified MPEG-4 encoder and the other stream, called auxiliary stream, is coded by predicting from its corresponding image, its previous image or its follow image.

• Horticulture, Environment, and Biotechnology : HEB
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• v.59 no.6
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• pp.857-864
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• 2018

Previous studies have not detected transcripts of the gene encoding anthocyanidin synthase (ANS) in white pomegranates (Punica granatum L.) and suggest that a large-sized insertion in the coding region of the ANS gene might be the causal mutation. To elucidate the identity of the putative insertion, 3887-bp 5' and 3392-bp 3' partial sequences of the insertion site were obtained by genome walking and a gene coding for an expansin-like protein was identified in these genome-walked sequences. An identical protein (GenBank accession OWM71963) isolated from pomegranate was identified from BLAST search. Based on information of OWM71963, a 5.8-Mb scaffold sequence with genes coding for the expansin-like protein and ANS were identified. The scaffold sequence assembled from a red pomegranate cultivar also contained all genome-walked sequences. Analysis of positions and orientations of these genes and genome-walked sequences revealed that the 27,786-bp region, including the 88-bp 5' partial sequences of the ANS gene, might be translocated into an approximately 22-kb upstream region in an inverted orientation. Borders of the translocated region were confirmed by PCR amplification and sequencing. Based on the translocation mutation, a simple PCR codominant marker was developed for efficient genotyping of the ANS gene. This molecular marker could serve as a useful tool for selecting desirable plants at young seedling stages in pomegranate breeding programs.

• Journal of information and communication convergence engineering
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• v.7 no.4
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• pp.501-506
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• 2009

In the H.264/ AVC video coding standard, the intra-prediction coding with various block sizes offers a considerably high improvement in coding efficiency compared to previous standards. In order to achieve this, H.264/AVC uses the Rate-distortion optimization (RDO) technique to select the best intraprediction mode for a macroblock, and it brings about the drastic increase of the computation complexity of H.264 encoder. To reduce the computation complexity and stabilize the coding performance on visual quality, this paper proposed a fast intra-prediction mode decision algorithm using non-parametric thresholds and simplified directional masks. The use of nonparametric thresholds makes the intra-coding performance not be dependent on types of video sequences and simplified directional masks reduces the compuation loads needed by the calculation of local edge information. Experiment results show that the proposed algorithm is able to reduce more than 55% of the whole encoding time with a negligible loss in PSNR and bitrates and provides the stable performance regardless types of video sequences.

• Parasites, Hosts and Diseases
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• v.54 no.2
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• pp.173-179
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• 2016

Echinostoma hortense (Digenea: Echinostomatidae) is one of the intestinal flukes with medical importance in humans. However, the mitochondrial (mt) genome of this fluke has not been known yet. The present study has determined the complete mt genome sequences of E. hortense and assessed the phylogenetic relationships with other digenean species for which the complete mt genome sequences are available in GenBank using concatenated amino acid sequences inferred from 12 protein-coding genes. The mt genome of E. hortense contained 12 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 1 non-coding region. The length of the mt genome of E. hortense was 14,994 bp, which was somewhat smaller than those of other trematode species. Phylogenetic analyses based on concatenated nucleotide sequence datasets for all 12 protein-coding genes using maximum parsimony (MP) method showed that E. hortense and Hypoderaeum conoideum gathered together, and they were closer to each other than to Fasciolidae and other echinostomatid trematodes. The availability of the complete mt genome sequences of E. hortense provides important genetic markers for diagnostics, population genetics, and evolutionary studies of digeneans.

• Journal of Multimedia Information System
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• v.8 no.3
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• pp.147-158
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• 2021

Versatile Video Coding (VVC) is the latest video coding standard developed by Joint Video Exploration Team (JVET). In VVC, the quadtree plus multi-type tree (QT+MTT) structure of coding unit (CU) partition is adopted, and its computational complexity is considerably high due to the brute-force search for recursive rate-distortion (RD) optimization. In this paper, we aim to reduce the time complexity of inter-picture prediction mode since the inter prediction accounts for a large portion of the total encoding time. The problem can be defined as classifying the split mode of each CU. To classify the split mode effectively, a novel convolutional neural network (CNN) called multi-level tree (MLT-CNN) architecture is introduced. For boosting classification performance, we utilize additional information including inter-picture information while training the CNN. The overall algorithm including the MLT-CNN inference process is implemented on VVC Test Model (VTM) 11.0. The CUs of size 128×128 can be the inputs of the CNN. The sequences are encoded at the random access (RA) configuration with five QP values {22, 27, 32, 37, 42}. The experimental results show that the proposed algorithm can reduce the computational complexity by 11.53% on average, and 26.14% for the maximum with an average 1.01% of the increase in Bjøntegaard delta bit rate (BDBR). Especially, the proposed method shows higher performance on the sequences of the A and B classes, reducing 9.81%~26.14% of encoding time with 0.95%~3.28% of the BDBR increase.