• Genomics & Informatics
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• v.17 no.1
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• pp.2.1-2.12
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• 2019

Chronic obstructive pulmonary disease (COPD) is a type of progressive lung disease, featured by airflow obstruction. Recently, a comprehensive analysis of the transcriptome in lung tissue of COPD patients was performed, but the heterogeneity of the sample was not seriously considered in characterizing the mechanistic dysregulation of COPD. Here, we established a new transcriptome analysis pipeline using a deconvolution process to reduce the heterogeneity and clearly identified that these transcriptome data originated from the mild or moderate stage of COPD patients. Differentially expressed or co-expressed genes in the protein interaction subnetworks were linked with mitochondrial dysfunction and the immune response, as expected. Computational protein localization prediction revealed that 19 proteins showing changes in subcellular localization were mostly related to mitochondria, suggesting that mislocalization of mitochondria-targeting proteins plays an important role in COPD pathology. Our extensive evaluation of COPD transcriptome data could provide guidelines for analyzing heterogeneous gene expression profiles and classifying potential candidate genes that are responsible for the pathogenesis of COPD.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.47 no.5
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• pp.9-17
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• 2010

This paper presents a localization perception of a phantom sound image for ultrahigh-definition TV with respect to various loudspeaker configurations; two-horizontal, two-vertical and triplet loudspeakers. Vector base amplitude panning algorithm with modification for non-equidistant loudspeaker setup is applied to create the phantom sound image. In order to practically study the localization performance in real situation, the listening tests were conducted at the on-axis and off-axis positions of TV in normal listening room. A method of adjustment which can reduce the ambiguity of a perceived angle is exploited to evaluate the angles of octave-band signals. The subjects changed the panning angle until the real sound source and virtually panned source were coincident. A spatial blurring can be measured by examining the differences of the panning angles perceived with respect to each band. The listening tests show that the triplet panning method has better performance than vertical panning in view of perceptual localization and spatial blurring at both on-axis and off-axis positions.

• Journal of the Korea Institute of Military Science and Technology
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• v.25 no.3
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• pp.231-238
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• 2022

Mine detection has been mainly studied with images of the forward-looking sonar. Forward-looking sonar assumes the propagation path of the sound wave as a straight path, creating the surrounding images. This might lead to errors in the detection by ignoring the refraction of the sound wave. In this study, we propose a mine localization method that can robustly identify the location of mines in an underwater environment by considering the refraction of sound waves. We propose a method of estimating the elevation angle of arrival of the target echo signal in a single receiver, and estimate the mine location by applying the estimated elevation angle of arrival to ray tracing. As a result of simulation, the method proposed in this paper was more effective in estimating the mine localization than the existing method that assumed the propagation path as a straight line.

• 제어로봇시스템학회:학술대회논문집
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• 2003.10a
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• pp.1184-1189
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• 2003

Map building and position estimation capabilities are practically indispensable for a mobile robot to execute its given tasks in its working environments. An autonomous map building method and a smart localization method is proposed in our previous works. The experimental verifications are carried out in this paper. We applied the proposed algorithms to mobile service robots in large-scale indoor buildings. Experimental results show that our strategy is reliable and feasible in tough conditions like non-polygonal and dynamic environments. The advantages of the algorithms are well-illustrated through real experiments.

• Journal of Multimedia Information System
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• v.5 no.3
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• pp.179-188
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• 2018

In order to overcome the problems of contact palmprint recognition, a non-contact palmprint recognition system is developed on personal computer (PC) platform. Three methods, namely "double-line-single-point" (DLSP), "double-assistant-crosshair" (DAC) and "none-assistant-graphic" (NAG), are implemented for the palmprint localization to solve the severe technical challenges, including the complex background, variant illuminations, uncontrollable locations and gestures of hands. In NAG, hand segmentation and the cropping of region of interest are performed without any assistant graphics. The convex hull contour of hand helps detect the outside contour of little finger as well as the valley bottom between thumb and index finger. The three methods of palmprint localization have good operating efficiency and can meet the performance requirements of real-time system. Furthermore, an attendance system on PC platform is designed and developed based on non-contact palmprint recognition.

• Proceedings of the IEEK Conference
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• 2000.07a
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• pp.253-256
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• 2000

This paper describes a DSP implementation of a real-time 3D sound localization algorithm with the use of a low power embedded DSP. A distinctive feature of this implementation is that the audible frequency band is divided into three, in accordance with the sound reflection and diffraction phenomena through different media from a certain sound source to human ears, and then in each subband a specific implementation procedure of the 3D sound localization is devised so as to operate real-time at a low frequency of 50MHz on a 16bit fixed-point DSP. Thus out DSP implementation can provide a listener with 3D sound effects through a headphone at low cost and low power consumption.

• Proceedings of the IEEK Conference
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• 2000.07a
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• pp.264-267
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• 2000

This paper describes a real-time 3D sound localization algorithm to be implemented with the use of a Bow power embedded DSP. This algorithm first divides the audible frequency band into three, on the basis of the analysis of the sound reflection and diffraction effects through different media from a certain sound source to human ears, and then in each subband a specific procedure is devised fur the 3D sound localization so as to operate real-time on a low power embedded DSP This algorithm aims at providing a listener with the 3D sound effects through a headphone at low cost and low power consumption.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.4
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• pp.2447-2451
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• 2013

Objective: To study changes of tumor associated carbohydrate antigen (TACAs) expression and mRNA levels for tumor associated glycosyltransferases, and assess subcellular localizations of N-acetyl galactosyltransferases (GalNAc-Ts) in the K562 leukemia cell line after imatinib treatment. Methods: RT-PCR was performed to analyze the expression of glycosyltransferases which synthesize O-glycan in tumor-associated carbohydrate antigens (TCTAs). The expression of Tn antigen, T antigen and sialyl T antigen on K562 cell membranes was measured by flow cytometry after treatment with different concentrations of imatinib. Co-localization of GalNAc-Ts and ER (endoplasmic reticulum) was determined by confocal laser scanning microcopy. Results: Transcript expression levels of several glycosyltransferases related to TCTAs were decreased after imatinib ($0-0.3{\mu}M$) treatment. Expression of Tn antigen and T antigen was increased while that of sialyl T antigen was decreased. Co-localization of GalNAc-Ts and ER was reduced by $0.2{\mu}M$ of imatinib. Conclusion: Imatinib inhibited the expression of O-glycan related TACAs and several related glycosyltransferases, while decreasing the co-localization of GalNAc-Ts and ER and normalizing O-glycosylation in the K562 human leukemia cell.

• Molecules and Cells
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• v.26 no.1
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• pp.100-105
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• 2008

Glycogen synthase kinase $3{\beta}$ (GSK $3{\beta}$) is a serine/threonine kinase that phosphorylates substrates such as ${\beta}$-catenin and is involved in a variety of biological processes, including embryonic development, metabolism, tumorigenesis, and cell death. Here, we present evidence that human GSK $3{\beta}$ is associated with Fe65, which has the characteristics of an adaptor protein, possessing a WW domain, and two phosphotyrosine interaction domains, PID1 and PID2. The GSK $3{\beta}$ catalytic domain also contains a putative WW domain binding motif ($^{371}PPLA^{374}$), and we observed, using a pull down approach and co-immunoprecipitation, that it interacts physically with Fe65 via this motif. In addition, we detected co-localization of GSK $3{\beta}$ and Fe65 by confocal microscopy, and this co-localization was disrupted by mutation of the putative WW domain binding motif of GSK $3{\beta}$. Finally, in transient transfection assays interaction of GSK $3{\beta}$ (wt) with Fe65 induced substantial cell apoptosis, whereas interaction with the GSK $3{\beta}$ AALA mutant ($^{371}AALA^{374}$) did not, and we noted that phosphorylation of the Tyr 216 residue of the GSK $3{\beta}$ AALA mutant was significantly reduced compared to that of GSK $3{\beta}$ wild type. Thus, our observations indicate that GSK $3{\beta}$ binds to Fe65 through its $^{371}PPLA^{374}$ motif and that this interaction regulates apoptosis and phosphorylation of Tyr 216 of GSK $3{\beta}$.

• Progress in Medical Physics
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• v.11 no.1
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• pp.39-47
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• 2000

In Stereotactic Radiosurgery (SRS), there are three imaging methods of target localization, such as digital subtraction Angiography (DSA), computed tomography (CT), magnetic resonance imaging (MRI). Especially, DSA and MR images have a distortion effect generated by each modality. In this research, image properties of DSA were studied. A first essential condition in SRS is an accurate information of target locations, since high dose used to treat a patient may give a complication on critical organ and normal tissue. Hut previous localization program did not consider distortion effect which was caused by image intensifier (II) of DSA. A neurosurgeon could not have an accurate information of target locations to operate a patient. In this research, through distortion correction, we tried to calculate accurate target locations. We made a grid phantom to correct distortion, and a target phantom to evaluate localization algorithm. The grid phantom was set on the front of II, and DSA images were obtained. Distortion correction methods consist of two parts: 1. Bilinear transform for geometrical correction and bilinear interpolation for gray level correction. 2. Automatic detection method for calculating locations of grid crosses, fiducial markers, and target balls. Distortion was corrected by applying bilinear transform and bilinear interpolation to anterior-posterior and left-right image, and locations of target and fiducial markers were calculated by the program developed in this study. Localization errors were estimated by comparing target locations calculated in DSA images with absolute locations of target phantom. In the result, the error in average with and without distortion correction is $\pm$0.34 mm and $\pm$0.41 mm respectively. In conclusion, it could be verified that our localization algorithm has an improved accuracy and acceptability to patient treatment.