• 한국작물학회지
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• 제51권3호
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• pp.220-226
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• 2006

필름 포장 상태로 유통되는 콩나물의 주요 상품성 저하 원인 중 하나인 이취의 종류 및 발생 조건 등을 구명하고자 수행된 실험 결과를 요약하면 다음과 같다. 1. 콩나물은 저장 온도가 높을 수록 호흡속도가 증가하여 $O_2$의 빠른 소모와 높은 농도의 $CO_2$ 축적 및 알콜취, 이취의 발생 촉진을 가져왔다. 2. 알콜취는 이취에 앞서 발생하였으며 EtOH은 이취와 유사한 시점에서 GC를 이용한 검출이 가능하였다. 3. 이취의 발생 한계 $O_2$ 농도는 $1{\sim}2%$ 수준으로, 알콜취의 발생 한계 $O_2$ 농도는 $4{\sim}2%$ 수준인 것으로 나타났다. 4. 이취 기체 내에는 다양한 종류의 aldehyde류 및 fatty acid류가 동정되었다.

• 대한바이러스학회지
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• 제29권4호
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• pp.235-245
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• 1999

HIV-1 Tat, a strong transactivator, is essential for the HIV-1 replication and AIDS progression. The Tat function is markedly inhibited by human p53 anti-oncogene. However, the detail mechanism has not yet been clearly revealed. In our previous report, we have addressed that p53 is unlikely to interact directly with HIV-1 Tat. In the consecutive experiments, Tat-phosphorylation was found to increase in proportional to the amounts of transfected p53. This work was initiated to identify the signaling factor that is involved in the p53-mediated Tat suppression. Several protein kinases were tested for the phosphorylation of Tat, and we found that PKR is likely to be involved in the p53-mediated Tat suppression. PKR was co-immunoprecipitated by anti-Tat antibody in the Tat-expressing Jurkat cell lysates only when the cells were transfected by p53, indicating that PKR-Tat interaction depends on the p53 activity. The interaction seems to result in PKR-mediated Tat-phosphorylation. Tat function was not blocked by p53 when co-transfected trasiently with antisense-PKR. We have generated PKR-knock out Jurkat cell clone. The PKR defective Jurkat cells didn't show the p53-mediated Tat suppression. These data indicate that p53-mediated Tat suppression is strongly associated with PKR. PKR-mediated Tat phosphorylation experiments are now under investigation by kinase assay and co-immunoprecipitation in the presence or absence of p53.

• Journal of Genetic Medicine
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• 제9권1호
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• pp.42-46
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• 2012

Short-chain acyl-CoA dehydrogenase deficiency (SCADD; OMIM # 201470) is an autosomal recessive inborn error of mitochondrial fatty acid ${\beta}$-oxidation, presenting with a variety of clinical signs and symptoms. Developmental delay, hypertonia or hypotonia, ketotic hypoglycemia, and epilepsy are most frequently reported. In general, patients diagnosed through newborn screening have shown normal growth and development in contrast to those diagnosed as a result of clinically initiated evaluations. Here, the case of an asymptomatic Korean newborn with SCADD identified by tandem mass spectrometry is reported. The patient showed an elevated concentration of butyrylcarnitine detected on newborn screening. Urinary excretion of ethylmalonic acid was elevated by urine organic acid analysis. To confirm the diagnosis of SCADD, a direct sequencing analysis of 10 coding exons and the exon-intron boundaries of the ACADS gene were performed. Genetic analysis of ACADS showed the following novel compound heterozygous missense mutations: c.277C>A (p.Leu93Ile) on exon3 and c.682G>A (p.Glu288Lys) on exon6. These results will provide further evidence of mutational heterogeneity for SCADD.

• 한국기후변화학회지
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• 제8권3호
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• pp.221-230
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• 2017

There are two main policies to meet the national goal of reducing Greenhouse Gases (GHGs) emissions in Korea towards Paris Agreement. From 2012 to 2014, Target Management System (TMS) was operated and the Emissions Trading Scheme (ETS) has been established since 2015. To compare the impact of TMS and ETS on reducing GHGs, we collected annual GHGs emission reports submitted by individual business entities, and normalized them using a z-variant normalized function. In order to evaluate the impact of those policies, we calculated the amount of GHGs emissions of 73 business entities from 15 business sectors. Those entities emitted $508million\;CO_2eq$, which is 74% of total national GHGs emissions in 2014. The main results of analysis indicate that accumulated GHGs emissions during the period 2012 to 2014 affected by TMS was higher than the national goal of GHGs emission reduction, and only the GHGs emissions in 2014 were in the range of allowed GHGs emissions, set by the Government. In 2015, when ETS initiated, total GHGs emission trading was $4.84million\;tCO_2eq$, which is only 0.9% of total allowance in 2015. However, more than 50% of business entities, who got the allowance of GHGs emission given by the Government, met the goal of GHGs emissions. Particularly, 27 of 73 business entities reduced GHGs more under the ETS rather than the TMS. Even though we analyzed only 4 years' data to demonstrate the impact of TMS and ETS, it is expected to commit the national goal of GHGs reduction target by TMS and ETS.

• 통합자연과학논문집
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• 제2권1호
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• pp.18-23
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• 2009

This minireview provides the chosen examples of our recent discoveries in the polymerization of hydrosilanes, dihydrosilole, lactones, and vinyl derivatives using various catalysts. Hydrosilanes and lactones copolymerize to give poly(lactone-co-silane)s with $Cp_2MCl_2$/Red-Al (M = Ti, Zr, Hf) catalyst. Hydrosilanes (including dihydrosilole) reduce noble metal complexes (e.g., $AgNO_3$, $Ag_2SO_4$, $HAuCl_4$, $H_2PtCl_6$) to give nanoparticles along with silicon polymers such as polysilanes, polysilole, polysiloxanes (and silicas) depending on the reaction conditions. Interestingly, phenylsilane dehydrocoupled to polyphenylsilane in the inert nitrogen atmosphere while phenylsilane dehydrocoupled to silica in the ambient air atmosphere. $Cp_2M/CX_4$ (M = Fe, Co, Ni; X = Cl, Br, I) combination initiate the polymerization of vinyl monomers. In the photopolymerization of vinyl monomers using $Cp_2M/CCl_4$ (M = Fe, Co, Ni), the photopolymerization of MMA initiated by $Cp_2M/CCl_4$ (M = Fe, Co, Ni) shows while the polymerization yield decreases in the order $Cp_2Fe$ > $Cp_2Ni$ > $Cp_2Co$, the molecular weight decreases in the order $Cp_2Co$ > $Cp_2Ni$ > $Cp_2Fe$. For the photohomopolymerization and photocopolymerization of MA and AA, the similar trends were observed. The photopolymerizations are not living. Many exciting possibilities remain to be examined and some of them are demonstrated in the body of the minireview.

• 법제연구
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• 제44호
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• pp.211-239
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• 2013

우리정부는 AFoCO 협정의 이행을 위한 상설 행정조직으로서 AFoCO 사무국을 국내에 유치하였다. 그럼에도 불구하고 우리 정부는 공식적으로 국제기구를 인정하는 기준을 마련하고 있지 않은 상태이며, 국제기구가 국내에서 법인격을 가지고 활동할 수 있는 국내법적 근거나 정부부처 차원의 국제기구 등록이나 국제기구 공인을 위한 법제도적 장치가 없는 현실이다. 이 글은 AFoCO 사무국의 국제기구로서의 지위를 설명하는데 필요한 국제법 이론과 국제기구의 국내법적 지위와 관련된 미국과 일본 등 선진국의 국제기구관련 국내 법제를 실증적으로 검토하였다. 이를 통해 우리나라의 글로벌화 추세 속에 향후 지속적으로 추진될 국제기구의 국내유치에 따른 국제기구 사무국의 국제법적 지위와 국내법인격의 문제의 해결을 위한 시사점을 찾고자 하였다. AFoCO 사무국은 한국과 아세안 회원국정부가 당사국이 되어 합의를 도출하고 국제법의 규율을 받는 조약으로 성립된 AFoCO 협정을 체결하여 설립되었다는 점, 국제기구는 당해 기구의 설립을 위한 국제법적 문서에 의하여 명시적으로 법인격이 부여되지 않아도 설립목적의 달성을 위해 필요한 범위 내에서 법인격을 보유한다는 묵시적 권한이론 등을 고려할 때 국제기구로서의 지위를 가지며 법인격을 향유한다. 따라서 AFoCO 사무국은 산림분야에 있어서 한국과 아세안 국가들 간의 협력사업 수행을 목표로 하는 행정적 기관으로서 한국과 아세안 회원국들이 당사국인 다자간 지역 국제기구라고 할 수 있다. 하지만 우리 정부가 글로벌 시대에 부응하여 좀 더 많은 국제기구를 적극 유치하고자 한다면 유치하는 국제기구에 대하여 사례별로 또는 국제법 이론에 근거하여 국제기구로서의 지위를 갖도록 할 것이 아니라 외교부 소관의 법률로 가칭 '국제기구 유치지원에 관한 법률'을 제정하여 명시적인 국내법 규정에 근거하여 국내 유치 국제기구에 대한 법적 지위를 부여할 뿐만 아니라 국제기구를 국내에 적극적으로 유지하도록 지원하는 방안을 모색해야 할 것이다.

• 한국산림과학회지
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• 제97권5호
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• pp.547-551
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• 2008

The study aimed to develop a cryopreservation method for long-term storage using mature somatic embryo of Japanese larch. In this study, desiccation treatments significantly affected re-induction rates of embryogenic tissue (ET) from dried somatic embryos. In the effect of different dehydration temperature and duration on the re-initiation ET. the highest frequency was shown when somatic embryos were dehydrated at $25^{\circ}C$ for 2 (45.5%) or 1 day (43.3%), respectively. In addition, low temperatures [$4^{\circ}C$, 2 days (44.2%) or 3 days (43.5%)] were marked higher ET initiation. After that, the initiation value was declined with dehydration duration. For comparison of different relative humidity on re-induction frequency of ET, the best re-induction (43.5%) was obtained from somatic embryos pre-dried at $(NH_4)_2SO_4$ (RH 79%). Both $Na_2HPO_4$ (RH 97%) and $Na_2CO_3$ (RH 88%) treatments were showed the similar rate, 34.6, 34.2%, respectively. However the lowest rate (19.6%) was observed in distilled water (RH 100%). In comparison of the various storage temperatures and duration of the dried somatic embryos, the highest frequency (66.9%) of re-initiation was obtained when somatic embryos were cryopreserved for one day. However, the frequency was gradually decreased as the time length of storage increased regardless of types of storage. None of ET re-initiated when stored at $4^{\circ}C$ for 1, 2 and 84 days.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1997년도 하계학술대회 논문집 F
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• pp.2075-2077
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• 1997

An electronic starter using MOSFET is developed to take advantage of ideal preheating and starting features which can extend the lifetime of fluorescent lamps. The preheating curcuit of the developed electronic starter is consisted of three parts - a full wave rectifier curcuit, an FET switching curcuit, and a timer curcuit for the gate switching. The curcuit allows sufficient preheating current flow before the starting to protect lamp filaments, nevertheless it shortens the Preheating time and enables a single pulse ignition at the peak level of the line voltage. Experimental results show that fluorescent lamps of 20-40W range can be initiated within rather short time of $1{\sim}1.5sec$ with preheating current of 0.6A. The electronic starter withstands more than 70.000 cycles switchings without noticeable blackening due to anode spot. These features provide Proper evidences for the advantage of direct replacement with the new starter.

• 한국시스템다이내믹스연구
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• 제9권2호
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• pp.77-103
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• 2008

In order to respond dynamic changes in the global distribution system, the City of Pyeongtaek hope to expand port facilities and its hinterlands. In specific, it plans to designate these areas as free economic zones(FEZ), synthesizing the whole procedures from the development proposals to the operation and management. This paper examines the proposed urban development strategies focused on Pyeongtaek port which still reveals critical weakness as a gateway port backed up by the Seoul Metropolitan Areas. In order to enhance the global competitiveness of Pyeongtaek port, theoretically it depends on Geo Coyle's ACTIFELD methods. In addition to the traditional quantitative data, Coyle's ACTIFELD methods emphasize qualitative approaches, all of which would facilitate structural and systemic analyses of complex social phenomena. Applying ACTIFELD methods, this paper proposes that the City of Pyeongtaek should focus on three alternatives. That is, Pyeongtaek's policy priority should be given to ts he port city based on the key concepts of Supply Chain Management(SCM), Co-opetition supporting city, and waterfront-initiated urban development.

• 한국연초학회지
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• 제13권1호
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• pp.43-52
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• 1991

These studies were carred out to obtain the transformant from tobacco cells by Agrobacterium spp. from crown gall and soil at the natural field in Korea, and identified their virulence. Kodo's and Clark's selective media were used for isolation of Agrobacterium spp. In these media, total of 99 strains were characterized based on the morphological characteristics of colonies. Among them 34 strains were able to induce on carrot discs. And hypervirulent strains C23-1 and K29-1 were identified as Agrobacterium tumefaciens biotype 1 and biotype 2, respectively. These strains formed fast growing, larger gall as compared to those induced by other strains on the carrot discs. Transformed tobacco callus was initiated on the phytohormone free MS medium with 250$\mu\textrm{g}$/ml carbenicillin after co-cultivation of tobacco stem explants and Agrobacteria. On the phytohormone free media, shoot was rarely formed from transformed callus. However, these shoot were teratoma shoots which were not grown as normal shoot, and teratoma shoot from transformant by C23-1 was smaller than that of K29-1.