• 대한지역사회영양학회지
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• 제10권2호
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• pp.183-188
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• 2005

The aim of this study was to assess the effect of 6-week vegetable juice supplementation (360 ml/day) on serum lipid profiles and antioxidant activity in college women. Twenty women (mean age: 21) with normal life style and dietary pattern and who are free of any specific diseases were recruited among the student in S women's university. The subjects consumed vegetable juice to take part in an uncontrolled clinical trial for a 6-week intervention period. While there was no difference in the concentration of total cholesterol and HDL-cholesterol, serum concentrations of TG and HDL/LDL ratio were significantly reduced by $22\%$ (p = 0.013) and $6\%$ (p =0.007) respectively. Significant decrease in manlondialdehyde (p = 0.000) was accompanied by an increase in the activity of serum antioxidant enzymes, such as GSHPx (p = 0.000), SOD (p=0.007). It was also found that total antioxidant status was improved by $5.4\%$ (p=0.009). Serum parameters were all changed without affecting body mass index, waist and hip circumferences, or nutrient intakes after six week of supplementation. This study demonstrated that the supplementation regular meals with vegetable juice can favorably affect serum lipid profiles and antioxidant systems, and hence could contribute to reduce the risks of chronic diseases in college women.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권5호
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• pp.442-448
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• 2006

Human lactoferrin (hLf) is an iron-binding glycoprotein that has been considered to play many biological roles in the human, including the stimulation of the immune system, antimicrobial and anti-inflammatory effects, and regulation of iron absorption. We generated transgenic Siberian ginseng (Acanthopanax senticosus) cell cultures producing a functional hLf protein using the signal peptide sequence from the endoplasmic reticulum and driven by an oxidative stress-inducible SWPA2 promoter which is highly expressed in plant cell cultures. The production of hLf increased proportionally to cell growth and showed a maximal level (up to 3.6% of total soluble protein) at the stationary phase in suspension cultures. Full-length hLf protein was identified by immunoblot analysis in transgenic cell cultures of Siberian ginseng. Recombinant hLf (rhLf) was purified from suspension cells of Siberian ginseng by ammonium sulfate precipitation, cation-exchange and gel filtration chromatography. N-terminal sequences of rhLf were identical to native hLf (nhLf). The overall monosaccharide composition of rhLf showed the presence of plant specific xylose while sialic acid is absent. Antibacterial activity of purified rhLf was higher than that of nhLf. Taken together, we anticipate that medicinal Siberian ginseng cultured cells, as demonstrated by this study, will be a biotechnologically useful source for commercial production of functional hLf not requiring further purification.

• KSBB Journal
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• 제23권2호
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• pp.153-157
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• 2008

생리활성이 뛰어난 마카의 지질 분획으로부터 macamides를 분리 동정하기 위해 본 연구에서는 초임계 이산화탄소 추출을 통해 마카 지질 추출물을 제조하여 재순환 분취용 HPLC로부터 2종의 macamides 물질을 분리 정제하였다. 분리된 2종의 macamides 물질의 구조 해석을 위해 LC/MS와 $^1H$-NMR과 $^{13}C$-NMR 분석을 실시한 결과 분자량이 384인 N-benzyl-5-oxo-6E,8E-octadecadienamide와 분자량이 346인 N-benzylhexadecanamide로 동정되었다. 본 연구를 통해 분리된 2종의 macamides는 향후 마카분말 및 마카제품의 macamides의 지표물질로 활용이 가능할 것으로 사료된다.

• Journal of Periodontal and Implant Science
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• 제29권4호
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• pp.995-1005
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• 1999

Alveolar bone destruction is a character-istic of periodontal disease. Treponema denticola are found in significantly increased numbers in the sites affected with periodontal disease. In order to clarify the role of T. denticola in destruction of alveo-lar bone in periodontal disease, this study was undertaken to determine the effect of sonicated extract of T. denticola on osteo-clast differentiation in co-culture system of mouse bone marrow cells and calvaria cells. The ability of osteoclast formation was estimated by counting the number of tar-tartrate resistant acid phosphatase(TRAP) positive cells. Sonicated extract of this bacteria stimulated osteoclast formation in a dose dependent manner(p<0.05). Indomathacin, an inhibitor of prostaglandin synthesis, decreased osteoclast formation induced by sonicated extract of this bacte-bacteria(p<0.05). Extract-induced osteoclast formation was decreased, when sonicated extract of bacteria was heated(p<0.05). These findings suggest that T. denticola induces osteoclast differentiation, and protein component of this bacteria and $PGE_2$ may play an important role in this process.

• 패션비즈니스
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• 제22권2호
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• pp.61-73
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• 2018

Power is the driving force of society, and the generation of power is inevitable. as long as society is rganized hierarchically. According to Michael Foucault's discourse modern power operates as a mechanism of 'panopticon', a system that monitors the 'body' of man through discipline. Moreover. fashion as acts as a symbol of beauty that continues to co-exist with power for the purpose of exposing status and authority, and for displaying the trends within a culture. So, it is necessary to study fashion design according to the changing power structure that exists in society. The aim of this study is to suggest types of creative fashion design process by visualizing the Foucault's power discourse through the image associative action. The four types of creative fashion design that have been drawn by visualizing Foucault's power discourse are as follow: disciplinary power, imprisonment power and knowledge power. The first type of fashion design method is to emphasize the shoulder by using shoulder pads, strings, tabs, and incisions in the clothing. The second method is to expose the body by using see-through material and manipulating its composition to expose the body. Third method is to borrowing elements of underwear. Fourth method is to utilize patterns that represent power, such as weapons, bones, blood, muscles, skulls, and various human imagesin the clothing. Through this study we expect to utilize creative fashion design to visualize concepts of the humanities, such as philosophical discourse.

• 한국간호교육학회지
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• 제8권1호
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• pp.108-119
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• 2002

This paper as the basic study aiming the establishment of nursing theory according to Korean culture, is made to reveal the types of recognition about concepts of health immanent in Korean who have the health view, completely mixed with traditional oriental medicine and Confucianism and Buddhism and Shamanism and european scientific medicine, etc. This paper is using the Q-method, which is greatly effective in measuring the individual subjectivity, to collect rightly the concepts of health of objects. This analysis shows us the 4 types of recognition about concepts of health immanent in Korean as follows. Type Ⅰ: modern self-searching type(現代的 自己 追求型), Type Ⅱ: nature-sharing type(自然 共有型), Type Ⅲ: meaning-intentional altruism(意味 指向的 他者 中心型), Type Ⅳ: oriental adaptational type(東洋的 順應型). The men belonging to type Ⅰ have positive and self-searching view of health. They are not only interpreting the concept of health scientifically, but also positively accepting the oriental concept of health. The men belonging to type Ⅱ have strong tendency of down-to-earth. They not only have the naturalistic view of health as Taoist, but also carry the weight in the family and neighbour and regard co-sharing as excellent virtue. The men belonging to type Ⅲ are positive altruists, pursuing the meaning of life and at the same time setting the importance to the good value system. Naturally they care more the family and neighbour than themselves. The men belonging to type Ⅳ have oriental view of the world characterized by nature-adaptation. They positively agree to the Shamanism, Buddhism, Confucianism and Oriental concept of health. In conclusion, in all types we can see that Korean understand the harmony of man and nature as fundamental principle of heath and carry the more importance to the mind(心) than body(身) and at the same time esteem more the family-centric, oriental and organic community than individual. All above facts is immersed in the concepts of health of Korean as a common denominator.

• 한국미생물·생명공학회지
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• 제28권5호
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• pp.279-284
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• 2000

This study was per-formed to screen lactic acid bacteria poultry for the probiotic use. Among the previously obtained acid tolerant, 139 strains, 111 strains were selected with MRS medium containing 0.3% oxgall. 34 strains of 111 was re-selected by Gram-staining and acid producing ability. These strains was identified by MIDI Sherlock Microbial Identification System. Among the identified 34 strains Lactobacillus fermenum YL-3 was selected for the final pro-biotic use because of the good growth and high survival rate at pH 2.0. 60%, 50% and 40% cells of Lactobacillus fermentum YL-3 survived at pH 3.0, 2.5 and 2.0, respectively. More than $10^{7}$ / CFU/ml survived when exposed with the number of $10^{8}$ CFU/ml at pH 2.0 after 12 hr. L.fermenum YL-3 maintained growth in MRS broth containing 0.3, 0.5, 1.0 and 2.0% oxgall for 24 hr. L.fermenum YL-3 showed an inhibitory effect against pathogenic strains of Sal. enteritidis and E. coli O157:H7. In mixed culture with L.fermenum YL-3 Sal. enteritidis lost ability com-pletely in 15 hrs and E. coil O157:H7 in 16 hrs.

• 한국항해항만학회지
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• 제34권1호
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• pp.83-89
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• 2010

본 논문은 일선파출소에 근무하는 해양경찰관들을 조사대상으로 하여 해양경찰 파출소 조직의 건강성 수준을 파악하고, 조직효과성에 영향을 미치는 조직건강성 측정지표들을 분석하고자 하였다. 연구의 결과 첫째, 해양경찰 파출소 조직의 건강성 수준은 업무스트레스를 제외한 모든 측정지표들에서 평균 이상인 것으로 나타나, 조직건강성 수준은 양호한 것으로 판단되었다. 둘째, 조직효과성에 의미있는 영향을 미치는 조직건강성 측정지표들은 개발문화, 보수, 동료관계, 승진제도, 감독, 직무자율성, 직무자체, 조직신뢰감, 업무지향성 등으로 나타났다. 특히 보수, 동료관계, 직무자율성, 직무자체, 조직신뢰감, 업무지향성 등의 측정지표들은 조직효과성에 긍정적인 영향을 미치는 것으로 나타났고, 현행 해양경찰조직의 개발문화, 승진제도, 감독 등은 일선파출소에 근무하는 해양경찰관의 조직효과성에 부정적인 영향을 미치는 것으로 나타났다.

• Journal of Microbiology
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• 제41권4호
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• pp.289-294
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• 2003

Gelatinase is a proteolytic enzyme that hydrolyzes gelatin. Gelatinolytic activity was detected from culture broths of Streptomyces griseus IFO13350 and HH1 by paper disc assays on 0.5% agar plates containing 1% gelatin. The concentrated extracellular protein from the S. griseus was analyzed by SDS polyacrylamide gel, and two proteins, with molecular weights of 30 and 28 kDa, respectively, were identified to have gelatinase activity by gelatin zymography. The protein with a molecular weight of 28 kDa was confirmed to be S. griseus trypsin (SGT). The effects of metal ions and metal chelators on the protease activity of the SGT were studied. Of the metal ions tested, only manganese was found to enhance the protease activity, 2.6 times, however, $Co^{2+},\;Cu^{2+},\;and\;Zn^{2+}$, and metal chelators, such as EDTA and EGTA, inhibited the SGT activity. When the protease activity of the SGT was measured at various pHs, in the presence of 5 mM $MnCl_2$, its highest activity was at pH 11.0, whereas only 60% of the maximum activity was observed between pHs 4.0 and pH 6.0, and almost 80% activity between pHs 7.0 to pH 10.0. The protease activity was measured at various temperatures in the presence of 5 mM $MnCl_2$. The SGT was found to be stable up to $60^{\circ}C$ for 30 min, while only 16% of the enzyme activity remained at $60^{\circ}C$, and at $80^{\circ}C$ almost all the activity was lost. The optimal temperature for the protease activity was $50^{\circ}C$.

• Restorative Dentistry and Endodontics
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• 제29권6호
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• pp.498-503
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• 2004

The properties of ideal root canal sealers include the ability of sealing the total root canal system and no toxic effects to periradicular tissues. Cytotoxicity test using cell culture is a common screening method for evaluation of the biocompatibility of root canal sealers. The purpose of this study was to investigate the cytotoxic effect of newly developed resin-based sealer (Adseal 1, 2, and 3) comparing with those commercial resin-based sealers (AH26 and AH Plus), ZOE-based sealers (Tubliseal EWT, Pulp Canal Sealer EWT) and calcium hydroxide based sealer (Sealapex), An indirect contact test of cytotoxicity by agar diffusion was performed according to the international standard ISO 10993-5. L929 fibroblast cells were incubated at $37^{\circ}C$ in humidified 5% $CO_2-containing$ air atmosphere. The freshly mixed test materials were inserted into glass rings of internal diameter 5 mm and height 5 mm placed on the agar. After the 24 hrs incubation period, the decolorization zones around the test materials were assessed using an inverted microscope with a calibrated screen. A Decolorization Index was determined for each specimen. Adseal 1. 2, and 3 did not exert any cytotoxic effects, whereas AH26, AH Plus, Tubliseal EWT, Pulp Canal Sealer EWT, and Sealapex produced mild cytotoxicity.