• BMB Reports
• /
• 제51권4호
• /
• pp.194-199
• /
• 2018

Mesenchymal stem cells (MSCs) have shown great potential in treating bone deficiency. Human adipose-derived stem cells (HASCs) are multipotent progenitor cells with multi-lineage differentiation potential. Human amnion-derived mesenchymal stem cells (HAMSCs) are capable of promoting osteogenic differentiation of MSCs. In this study, we investigated the effect of HAMSCs on HASCs by a transwell co-culture system. HAMSCs promoted proliferation, osteogenic differentiation, angiogenic potential and adiponectin (APN) secretion of HASCs. Moreover, the positive effect of HAMSCs was significantly inhibited by U0126, a highly selective inhibitor of extracellular signaling-regulated kinase 1/2 (ERK1/2) mitogen-activated protein kinase (MAPK) signaling pathway. These observations suggested that HAMSCs induced bone regeneration in HASCs via ERK1/2 MAPK signaling pathway.

• 대한생식의학회:학술대회논문집
• /
• 대한불임학회 2002년도 제42차 춘계학술대회
• /
• pp.93-94
• /
• 2002

• 산업경영시스템학회지
• /
• 제32권4호
• /
• pp.101-106
• /
• 2009

The unsafe act and unsafe condition is due to human error that experience 80% of safety accidents. Accordingly, one of the most important issues to reduce industrial accidents as a whole, is how to reduce the accident rate by the human error. Therefore, this paper describes the development of quantitative risk assessment (QRA) model by ergonomics for reform of safety consciousness on the semiconductor industry. Unconsciousness, disregard, ignorance, recklessness, and stress among the human factors are selected for conscious reform. Finally, the QRA model is efficiently expected to contribute towards improving continuous self-safety and health and safety culture campaign in order to prevent industrial accidents.

• Biomolecules & Therapeutics
• /
• 제32권2호
• /
• pp.231-239
• /
• 2024

Methyl anthranilate (MA) is a botanical fragrance used in food flavoring with unexplored potential in anti-pigment cosmetics. MA dose-dependently reduced melanin content without affecting cell viability, inhibited dendrite elongation and melanosome transfer in the co-culture system of human melanoma cells (MNT-1) and human keratinocyte cell line (HaCaT), and downregulated melanogenic genes, including tyrosinase, tyrosinase-related protein 1 and 2 (TRP-1, TRP-2). Additionally, MA decreased cyclic adenosine monophosphate (cAMP) production and exhibited a significant anti-pigmentary effect in Melanoderm^TM. These results suggest that MA is a promising anti-pigmentary agent for replacing or complementing existing anti-pigmentary cosmetics.

• Annals of Occupational and Environmental Medicine
• /
• 제35권
• /
• pp.18.1-18.7
• /
• 2023

South Korea has the highest policy priority for working hour regulations because it has longer annual working hours than other Organization for Economic Development Co-operation and Development countries and has fewer holidays. According to the results of the Working Conditions Surveys between 2006 and 2020, in 2020, 6% of wage earners worked for > 52 hours weekly. The percentage of workers exceeding 52 hours weekly has decreased over time; however, disparities exist based on age, industry, occupation, company type, and company size, particularly in service-, arts-, and culture-related occupations and workplaces with fewer than 5 employees. South Korea's working hours system is greatly influenced by the 52-hour weekly maximum; sometimes, a maximum of 64-69 hours, including overtime, is theoretically possible. To ensure healthy working hours, it is important to actively protect workers who fall through the cracks, such as those in businesses with fewer than 5 employees.

• 한국수산과학회지
• /
• 제44권3호
• /
• pp.237-242
• /
• 2011

To evaluate the proximate composition and main nutritive ingredients (vitamin A, $V_A$; vitamin E, $V_E$; cholesterol; the composition of fatty acids) between eels cultured in different domestic eel farms, we analyzed edible parts of eels that were cultured in three "Water Recirculation System (WRS)" farms and three "Still-Water System (SWS)" farms. We found differences in crude protein, crude lipids and the ash content of eel meat between the farms, but water content showed no significant difference. $V_A$ and $V_E$ contents did not show meaningful differences between the two culture methods, but there was a noticeable distinction between the eel farms. In particular, $V_E$ content showed significant differences between the eels of each farm (highest farm, about 25 mg/100 g dry wt; lowest farm, about 1 mg/100 g dry wt). The fatty acids of cultured eels were composed of monoenes (MUFA, 55-60%), saturates (SFA, 30-33%), $\omega$-6 highly unsaturates (HUFA, 1.6-2%) and $\omega$-3 HUFA (8-9.2%). The MUFA content of WRS eels ($59.9{\pm}1.97%$) was higher than that of SWS eels ($55.8{\pm}0.48%$). The contents of other fatty acids were higher in SWS eels (SFA, $33.0{\pm}0.52%$; $\omega$-6FUFA, $1.98{\pm}0.2%$; $\omega$-3 HUFA, $9.2{\pm}0.67%$) than in WRS eels (SFA, $30.5{\pm}1.34%$; $\omega$-6 HUFA, $1.62{\pm}0.19%$; $\omega$-3 HUFA, $8.0{\pm}0.5%$).

• 문화기술의 융합
• /
• 제6권4호
• /
• pp.703-708
• /
• 2020

최근 지진에 대한 위험이 고취됨에 따라 내진설계에 대한 관심이 높아지고 있는 실정이다. 이에 본 연구에서는 면진장치중 하나인 마찰 진자형 지진격리받침을 고강도 엔지니어링 플라스틱을 이용하여 슬라이더(spherical)를 적용하였다. 이는 다른 금속에 비해 가벼운 중량으로 운반, 설치 및 유지보수가 매우 용이하고, 기존의 금속부품 대비 내부식성이 매우 우수하다. 또한 특수 마찰재를 삽입하여 사용하므로 교체가 용이하여 마찰계수 조절이 가능하다는 장점을 입증하였지만 금속 소재 슬라이더 대비 장기 처짐에 대한 우려가 대두되어 왔다. 따라서 본 연구에서는 개발된 마찰 진자형 지진격리받침으로 압축-전단시험, 반복피로시험 후 극한하중 재하시험을 실시하여 시험 후의 압축 및 전단특성 변화와 반복피로시험 후 극한하중 재하시 슬라이더의 변형 및 전단특성을 측정, 분석하여 슬라이더의 장기 처짐값을 측정하였고 그 처짐값이 사용 설계 허용범위 내에 있는지를 입증하였다.

• Animal cells and systems
• /
• 제11권1호
• /
• pp.1-8
• /
• 2007

Recently, we have shown that some endocrine disruptors, heavy metals, organotins and azoles suppressed steroidogenic enzymes such as P450 side-chain cleavage enzyme (P450scc) and aromatase in bullfrog ovarian follicles. In the present study, by using an amphibian ovarian follicle culture system, we examined the effects of these endocrine disruptors on maturation and ovulation of oocytes from Rana dybowskii in vitro. Ovarian fragments or isolated follicles were cultured for 24 h in a medium containing frog pituitary homogenate (FPH) or progesterone ($P_{4}$) with or without endocrine disruptors, and oocyte maturation (germinal vesicle breakdown, GVBD) and ovulation were examined. Among the organotins, tributyltin (TBT) strongly inhibited both FPH-and $P_{4}-induced$ oocyte maturation ($ED_{50}$:0.6 and 0.7 ${\mu}M$, respectively); however, tetrabutyltin (TTBT) and dibutyltin (DBT) showed only partial suppression, while monobutyltin (MBT) showed no inhibitory effect. All of the organotins suppressed $P_{4}-induced$ oocyte ovulation very effectively at a low concentration, and TBT and DBT exerted an inhibitory effect on FPH-induced ovulation. Among the heavy metals, mercury (Hg), cadmium (Cd) and cobalt (Co) were very effective in inhibiting FPH-induced oocyte maturation and ovulation, while lead (Pb), arsenite (As) and zinc (Zn) were less effective. However, all of the heavy metals suppressed FPH-induced oocyte ovulation at a high dose ($100{\mu}M$). Among the azoles, itraconazole (ICZ), ketoconazole (KCZ) and clotrimazole (CTZ) effectively inhibited FPH-induced oocyte maturation and ovulation, while econazole (ECZ), miconazole (MCZ) and fluconazole (FCZ) were considerably less effective. These results demonstrated that the abovementioned endocrine disruptors exhibited differential effects on oocyte maturation and ovulation in amphibian follicles and that the frog ovarian culture system could be used as an effective experimental tool to screen and evaluate the toxicity of various endocrine disruptors in vitro.

• 문화기술의 융합
• /
• 제7권1호
• /
• pp.646-653
• /
• 2021

본 연구에서 개발된 의료용 침대는 N개의 건반으로 이루어져 각각이 엑츄에이터에 의해 수직으로 구동된다. 각 건반에는 M개의 센서가 실장되어 체압을 측정하므로 체압지도의 해상도는 MN에 의해 결정된다. 각 건반에는 센서 제어기가 실장되어 M개의 센서로부터 측정한 체압값을 CAN(Car Area Network)와 같은 직렬 통신 네트워크를 통해 메인제어기로 전달한다. 각 건반에는 모터를 구동하는 서보 드라이버가 하나씩 장착되며 메인 제어기와 CAN으로 연결되어 메인 제어기에서 지시된 변위값대로 건반의 높낮이를 제어하며 향후 개발될 인공지능 제어 알고리즘을 위해 신체 각부의 편안도를 조사하여 건반의 높낮이를 맞추었다. 또한, 건반 각부에 걸리는 최대 체압값과 체압비를 계산하여 인공지능에 의한 침대 편안도 제어를 위해 기초 데이터로 활용한다. 결과적으로 제시된 시스템은 향후 개발될 인공지능에 의한 신체의 안락함과 욕창 방지 제어를 위해 활용될 수 있는 토대가 될 수 있다.

• Korea Journal of Cosmetic Science
• /
• 제1권1호
• /
• pp.1-9
• /
• 2019

The identification of compounds with anti-senescence activity in cell culture system is a first step in aging research. Given that pyruvate can be used energy source by conversion to acetyl-CoA in mitochondria, and protects cultured cell from various stress-induced cell damage and cell death, synthetic media (e.g., DMEM) often includes 1 mM pyruvate, which is very higher than the pyruvate concentration in human blood (approximately 30 ��M). However, the use of medium containing high concentration of pyruvate is not suitable for screening anti-senescence compounds, because pyruvate also protects against the cellular senescence of primary human dermal fibroblasts (NHDFs) through NAD+ generated during conversion to lactate. In this study, four extracts, i.e., Sprouted seed and fruit complex, Poncirus trifoliata fruit extract, Jaum balancing complex, and Prunus mume extract were used for evaluation of different anti-senescence effect in the absence or presence of 0.1 mM pyruvate, similar to the physiological pyruvate concentration. The senescence in NHDFs cultured with DMEM in the presence of 0.1 mM pyruvate (approximately the physiological concentration in human blood) is accelerated, as observed in pyruvate deprivation conditions. The cytotoxicity of the Poncirus trifoliata fruit extract was protected by pyruvate, and Jaum balancing complex and Prunus mume extract had anti-senescence activity in the presence of 0.1 mM pyruvate, but not in the absence of pyruvate. Given that pyruvate is a powerful protector against both cytotoxicity and cellular senescence, the screening of candidate agents for anti-senescence in high pyruvate conditions using an in vitro cell culture system is not valid. Therefore, we recommend the use of a low concentration of pyruvate to evaluate the anti-senescence effects of candidates, which is more similar to in vivo aging conditions than excessive stress-induced senescence models, to exclude the effect of excessive pyruvate in vitro.