• Journal of the Korean Institute of Intelligent Systems
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• v.14 no.4
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• pp.402-410
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• 2004

In this paper, an optimal design method of clonal selection based Fuzzy-Neural Networks (FNN) model for complex and nonlinear systems is presented. The FNNs use the simplified inference as fuzzy inference method and Error Back Propagation Algorithm as learning rule. Also Advanced Clonal Selection (ACS) is proposed to find the parameters such as parameters of membership functions, learning rates and momentum coefficients. The proposed method is based on an Immune Algorithm (IA) using biological Immune System and The performance is improved by control of differentiation rate. Through that procedure, the antibodies are producted variously and the parameter of FNN are optimized by selecting method of antibody with the best affinity against antigens such as object function and limitation condition. To evaluate the performance of the proposed method, we use the time series data for gas furnace and traffic route choice process.

• Journal of Korean Society of Forest Science
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• v.83 no.2
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• pp.239-245
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• 1994

The broad sense heritability of height of clonal means was estimated from 18 clones out of 25 clones growing at four different sites, and it showed considerably high heritability of $H^2_{CM}=0.883$. Therefore if we select the best one clone(5% selection), the genetic gain of 35.3% which is equal to 1.02m in height is expected to attain. If we select the best two clones(10% selection), the genetic gain will be 25.6% which is equal to 0.74m in height. Because the trees are only 4-year-old, it is expected to show higher genetic gain than the current gain when the trees reach to the age of final selection.

• Journal of Institute of Control, Robotics and Systems
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• v.6 no.12
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• pp.1079-1085
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• 2000

In this paper, we propose a method of cooperative control (T-cell modeling) and selection of group behavior strategy (B-cell modeling) based on immune system in distributed autonomous robotic system (DARS). An immune system is the living bodys self-protection and self-maintenance system. these features can be applied to decision making of the optimal swarm behavior in a dynamically changing environment. For applying immune system to DARS, a robot is regarded as a B-cell, each environmental condition as an antigen, a behavior strategy as an antibody, and control parameter as a T-cell, respectively. When the environmental condition (antigen) changes, a robot selects an appropriate behavior strategy (antibody). And its behavior strategy is stimulated and suppressed by other robots using communication (immune network). Finally, much stimulated strategy is adopted as a swarm behavior strategy. This control scheme is based on clonal selection and immune network hypothesis, and it is used for decision making of the optimal swarm strategy. Adaptation ability of the robot is enhanced by adding T-cell model as a control parameter in dynamic environments.

• Korean Journal of Veterinary Research
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• v.39 no.6
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• pp.1112-1118
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• 1999

Galectin-3 plays an important role in cell development, differentiation and cancer metastasis, including cell-cell/extracellular matrix (ECM) interactions and is supposed to have an effect of apoptosis on T-cells in thymic clonal selection. In this study, to know the effect of galectin-3 on thymocyte development, we used recombinant human galectin-3 (rHgal-3) from Escherichia coli, JM105, which was inserted with human gal-3 gene-transformed plasmid vector (prGal-3) to express human galectin-3. Expressed rHgal-3 was confirmed by western blot using the culture supernant of hybridoma (M3/38) producing monoclonal antibody to human galectin-3. Sepharose gel affinity chromatography was used to purify the expressed rHgal-3. Thymocytes and hepatocytes from 6-week-old male BALB/c mice were incubated with rHgal-3 and showed marked increase of apoptotic population on analysis using flow cytometry with 7-AAD in a dosedependent manner. However, rHgal-3 failed to induce apoptosis on hepatocytes. Interestingly, this apoptotic effect was not inhibited by lactose, a specific lectin domain inhibitor. From these results, we concluded that extrinsic -3 induces apoptosis on mouse thymocytes, and galectin-3 may have an apoptotic effect on T-cells in thymic clonal selection.

• Korean Journal of Breeding Science
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• v.40 no.3
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• pp.263-268
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• 2008

Estimates of genetic gain (in volume growth) and diversity (expressed as status number, $N_s$) were determined in a clonal seed orchard of Pinus koraiensis. The genetic thinning was based on clonal breeding values (represented by general combining ability) obtained from progeny tests, clonal fertility estimated by strobilus production, and clonal size variation determined by the ramet numbers per clone. Parental GCA values for volume growth were calculated, based on height and diameter at breast height measured from field trials. Clonal fertility was estimated from the assessments of strobilus production over twelve years from 1991 to 2003, and used for the calculation of status number. There are 179 clones and 5,268 ramets in 12ha area of P. koraiensis clonal seed orchard. Genetic gain and diversity estimates were determined under assumptions of 30% pollen contamination and inferior genetic value of contaminating pollen. Genetic gain increased as thinning rates were set from 10% to 60%. However, for the higher thinning intensities, the increase of genetic gain was not remarkable. Genetic thinning by means of truncation selection resulted in a greater genetic gain but a large decrease in status number. Status number was represented around 40 clones for 10% through 60% thinning intensities, but for the higher thinning intensities, it was a bit fluctuated. Based on the present results, it could be concluded that thinning rate should not be stronger than 60% to optimize genetic gain while conserving genetic diversity. Consequently 50% or 60% thinning rate might be appropriate for genetic thinning in the clonal seed orchard of P. koraiensis. The effect of pollen contamination on the genetic gain and the consequence of genetic thinning for seed production in the clonal seed orchard, and seed orchard management scheme were also discussed.

• Fisheries and Aquatic Sciences
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• v.18 no.3
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• pp.287-296
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• 2015

Although rotifers have been considered the best feeding option for several species of fishes in aquaculture, they are sometimes larger than appropriate for the early larval stage of some marine fishes. Thus, we aimed to determine whether size-based selection of the parents could affect the average body size of their progeny in two clonal populations of the rotifer Brachionus rotundiformis. From each of the clones, 20 individuals were bi-directionally selected toward both smaller and larger sizes and each individual-based selection was conducted for 10 consecutive generations. The results showed that although there were sometimes differences in mean body size between parents and their progeny, no directional trend was observed in all selected lines of both clones. We demonstrated that artificial selection in a rotifer stock cannot lead to an expected size range although they appear to exhibit a large degree of body size polymorphism.

• Proceedings of the Korea Inteligent Information System Society Conference
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• 2001.01a
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• pp.298-309
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• 2001

This research aims to unravel the significant features of the human immune system, which would be successfully employed for a novel network intrusion detection model. Several salient features of the human immune system, which detects intruding pathogens, are carefully studied and the possibility and the advantages of adopting these features for network intrusion detection are reviewed and assessed.

• Development and Reproduction
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• v.4 no.2
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• pp.147-152
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• 2000

Four clonal lines of ayu, Plecoglossus altivelis, were produced through gynogenesis, mixed before hatching and reared communally. After 10 months, a randomly taken sample was subjected to a standardized shallow water stressor. Hematocrit, hemoglobin, red blood cells count (RBC) and mean corpuscular volume (MCV) were obtained from stressed and non-stressed fish. DNA fingerprinting was used to confirm the clonal nature of the organisms and to identified the clonal line to which each fish belonged. 1 observed significant differences between clona] lines mostly in the hematocrit and MCV measured under no-stress conditions. Such differences are suggested to represent mainly genetic variance, on account of the common environment provided to all the experimental groups. The stress response ratio was lower than expected, mainly due to some unexpectedly high non-stress values. Heritability values (h$^2$) were medium to high for the no-stress measurements (mean 0.238) and very low or zero for the stressed groups'traits (excepting one high value of 0.484). 1 conclude that the use of communally reared clonal lines represents a good tool for the characterization of the physiological traits, thus allowing for their utilization as genetic selection criteria.

• Journal of Veterinary Science
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• v.21 no.1
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• pp.2.1-2.14
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• 2020

The emergence of livestock-associated (LA)-methicillin-resistant Staphylococcus aureus (MRSA) in livestock animal has become a significant zoonotic concern. In the present study, we investigated nationwide prevalence of LA-MRSA across pork production chain including pig farms, slaughterhouses, and retail markets. A total of 40 MRSA strains were isolated during the investigation and the overall prevalence of MRSA was 3.4% (n = 37), 0.6% (n = 2), and 0.4% (n = 1) in pig farms, slaughterhouses, and retail markets, respectively. Multilocus sequence typing analyses revealed that the 2 most significant clonal lineages in pork production chain in Korea were ST398 (n = 25) and ST541 (n = 6). All of the 40 MRSA isolates were further characterized to investigate key genotypic and phenotypic correlates associated with the emergence and spread of clonal complex 398 (CC398; ST398, and ST541) LA-MRSA. Although the prevalence of swine-associated MRSA was still relatively low and mostly restricted to pig farms, multidrug-resistant CC398 LA-MRSA isolates with new spa types (t18102 and t18103) were identified as a major clonal lineage. The CC398 LA-MRSA strains tended to exhibit increased levels of multiple drug resistance (MDR) phenotype compared with non-CC398 MRSA strains. Of note, in comparison with non-CC398 MRSA isolates, CC398 LA-MRSA isolates exhibited significantly enhanced tetracycline (TET) and zinc resistance. These findings suggested that co-selection pressure associated with MDR phenotype, especially TET resistance, and zinc resistance may have played a significant role in the emergence and persistence of CC398 LA-MRSA in pig farms in Korea.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 1998.03a
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• pp.127-130
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• 1998

In this paper, we propose a method of cooperative control(T-cell modeling) and selection of group behavior strategy(B-cell modeling) based on immune system in distributed autonomous robotic system(DARS). Immune system is living body's self-protection and self-maintenance system. Thus these features can be applied to decision making of optimal swarm behavior in dynamically changing environment. For the purpose of applying immune system to DARS, a robot is regarded as a B cell, each environmental condition as an antigen, a behavior strategy as an antibody and control parameter as a T-call respectively. The executing process of proposed method is as follows. When the environmental condition changes, a robot selects an appropriate behavior strategy. And its behavior strategy is stimulated and suppressed by other robot using communication. Finally much stimulated strategy is adopted as a swarm behavior strategy. This control scheme is based of clonal selection and idiotopic network hypothesis. And it is used for decision making of optimal swarm strategy. By T-cell modeling, adaptation ability of robot is enhanced in dynamic environments.