• Journal of Microbiology
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• 제35권4호
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• pp.354-359
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• 1997

Methods for the extraction of DNA from water sample were approximated. Four different procedures of DNA extraction were carried out with pellets obtained from centrifugation of 4 liter water samples. The recovery efficiency and purity of DNA extracted by each method from different sources were compared. DNA yield varied with extraction methods, Method I, which involves enzymatic and freeze-thaw lysis steps and phenol and phenol-chloroform purification of extracted nucleic acid, showed a significantly higher yield and purity than the other methods. The use of glass beads in the DNA extraction methods improved the purity of DNA suitable for PCR. Bovine serum albumin in the PCR reaction mixture was useful in reducing inhibitory effects of contaminants. The efficiency of an extraction method was determined by the detection of the aer of Aeromonas hydrophila with PCR. The lower limit of detection of A. hydrophila from seeded tap water was 2 CFU/ml in PCR when method I was used for DNA preparation.

• The Korean Journal of Applied Statistics
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• 제10권2호
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• pp.353-366
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• 1997

In this paper, we derive an approximate optimal Bayes group sequential design for a given loss function. We use the Monte-Carlo method to compare the ASN(average sample size) function and Bayes risk of approximate optimal Bayes group sequential design, Pocock design and O'Brien and Fleming design. Also introduced is the concept of Bayes efficiency and percentage loss of information due to grouping for the group sequential design and use it to measure the loss of information for different group sizes.

• Communications for Statistical Applications and Methods
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• 제19권6호
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• pp.781-791
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• 2012

A $2{\times}2$ Cross-over design is widely used in clinical trials for comparison studies of two kinds of drugs or medical treatments. This design has many statistical methods such as Hills-Armitage's (1979) method or Koch's (1972) method. In this paper, we propose a nonparametric test for $2{\times}2$ Cross-over design based on a two-sample test suggested by Baumgartner et al. (1998). In addition, a Monte Carlo simulation study is adapted to compare the power of the proposed methods with those of previous methods.

• Proceedings of the KOSOMBE Conference
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• 대한의용생체공학회 1995년도 춘계학술대회
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• pp.127-130
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• 1995

Chromosome analysis is an important and difficult task for clinical diagnosis, for mutagen dosimetry, and for biological research. It is expensive, time consuming and imprecise when performed manually. Efforts lo automate some or all of the procedures have continued for more than 30 years, with only limited success. An acquiring sample from chromosome group is not solved with automatic method. It is still performed by user. This paper represents the method of an automatic chromosome sample extraction which based on region splitting, and scan converted method.

• Proceedings of the KIEE Conference
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• 대한전기학회 1995년도 하계학술대회 논문집 B
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• pp.661-663
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• 1995

Chromosome analysis is an important and difficult task for clinical diagnosis for mutagen dosimetry, and for biological research. It is expensive, time consuming and imprecise when performed manually. Efforts to automate some or all of the procedures have continued for more than 30 years, with only limited success. An acquiring sample from chromosome group is not solved with automatic method. It is still performed by user. This paper represents the method of an automatic chromosome sample extraction which based on region splitting, and scan converted method.

• Proceedings of the KOSOMBE Conference
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• 대한의용생체공학회 1996년도 추계학술대회
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• pp.251-253
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• 1996

Noninvasive monitoring of total Hemoglobin value is feasible with the use of spectroscopic measurements. As a step toward tile final goal of the development of a noninvasive monitor, the spectra$(400{\sim}800mm)$of EDTA whole blood were obtained along with reference total Hemoglobin values. Under the same condition water spectrum was generated. It was subtracted from each blood sample, and then tile first derivative of each subtracted data was taken by 'approximated first derivative algorithm' with gap (1,6,10,20nm). The correlation was obtained between total Hemoglobin and first valley wavelength of first derivative spectrum (sample number: 93).

• Journal of Korean Academy of Fundamentals of Nursing
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• 제14권1호
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• pp.29-43
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• 2007

Purpose: This study was done to develop a pain management protocol for nursing home patients with dementia and to examine effects of the protocol on pain assessments and interventions by the nurses and on pain relief signs in the patients. Method: The six steps in the protocol development and the examination of effect are outlined. Three rounds using the Delphi technique and one group pretest-posttest design experiment were developed. Design issues, such as sample selection and sample size, are addressed in relation to the study protocol. Results: After implementation of the pain management protocol, there were significant changes nursing actions including frequency of number of physical examinations, utilization of pain assessment tools, and request to doctors for discomfort management and there were significant changes in frequency in the number of verbal and physical expressions of pain, and emotional patterns. Conclusion: This is the first pain management protocol for patients with dementia in Korea. However, more study will be needed to determine the methodological strength and necessary revisions for the protocol.

• Journal of Veterinary Clinics
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• 제30권4호
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• pp.258-263
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• 2013

The primary goal of this study was to compute sample sizes required to achieve the each aim of a variety of foot-and-mouth disease (FMD) surveillance programs, using a statistically valid technique that takes the following factors into account: sensitivity (Se) and specificity (Sp) of diagnostic test system, desired minimum detectable prevalence, precision, population size, and desired power of the survey. In addition, sample sizes to detect FMD if the disease is present and also as proof of freedom were computed. The current FMD active surveillance programs consist of clinical, virological, and serological surveillance. For the 2012 serological surveillance, annual sample sizes (n = 265,065) are planned at four separate levels: statistical (n = 60,884) and targeted (n = 115,232) at breeding pig farms and slaughter house, in together with the detection of structural proteins (SP) antibodies against FMD (n = 88,949). Overall, the sample size was not designed taking the specific aims of each surveillance stream into account. The sample sizes for statistical surveillance, assuming stratified two-stage sampling technique, was based to detect at least one FMD-infected case in the general population. The resulting sample size can be used to obtain evidence of freedom from FMD infection, not for detecting animals that have antibodies against FMD virus non-structural proteins (NSP). Additionally, sample sizes for targeted surveillance were not aimed for the population at risk, and also without consideration of statistical point of view. To at least the author's knowledge, sampling plan for targeted, breeding pig farms and slaughter house is not necessary and need to be included in the part of statistical surveillance. Assuming design prevalence of 10% in an infinite population, a total of 29 animals are required to detect at least one positive with probability of 95%, using perfect diagnostic test system (Se = Sp = 100%). A total of 57,211 animals needed to be sampled to give 95% confidence of estimating SP prevalence of 80% at the individual animal-level with a precision of ${\pm}5%$, assuming 800 herds with an average 200 heads per farm, within-farm variance of 0.2, between-farm variance of 0.05, cost ratio of 100:1 of farm against animals. Furthermore, 779,736 animals were required to demonstrate FMD freedom, and the sample size can further be reduced depending on the parameters assumed.

• Journal of Korean Medicine for Obesity Research
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• 제13권1호
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• pp.1-9
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• 2013

Objectives: This study was performed to analyze the recent trend of the studies about obesity in medicine and to provide background for futher studies. Methods: All of the article were selected in "The Korean Journal of Obesity". Search were conducted through "http://kosso.or.kr" with the search word "obesity". Collected articles were classified into clinical study, experimental study, literary study, case report. Results: One hundred eighty four studies were included and analyzed in terms of study design, subject, intervention, period, obesity index and so on. The numbers of clinical studies, literary studies, experimental studies, case reports were respectively 143 (77.7%), 26 (14.1%), 2 (1.1%) and 7 (3.8%). In clinical studies, observational studies were 112 (78.3%) and intervention studies were 31 (21.7%). And most of sample sizes were more than 100 and less than 499. Body mass index, waist circumstance, body fat percent were major criteria of clinical study. Most of the subjects on obesity were about relation with another disease or experimental results and diagnosis. According to classification by the kinds of intervention, diet, exercise, drug, behavior were respectively 22, 18, 8, and 8. More than fourty percent of intervention studies were studied for 12 weeks. Conclusions: To improve the quality of Korean Medicine study for obesity, we need to recruiting big sample size and activate randomized clinical trial.

• Asian Pacific Journal of Cancer Prevention
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• 제14권7호
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• pp.4243-4247
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• 2013

The relationship between the X-ray repair cross-complementing group 3 (XRCC3) Thr241Met polymorphism and gliomas remains inclusive or controversial. For better understanding of the effect of XRCC3 Thr241Met polymorphism on glioma risk, a meta-analysis was performed. All eligible studies were identified through a search of PubMed, Elsevier Science Direct, Excerpta Medica Database (Embase) and Chinese Biomedical Literature Database (CBM) before May 2013. The association between the XRCC3 Thr241Met polymorphism and gliomas risk was conducted by odds ratios (ORs) and 95% confidence intervals (95% CIs). A total of nine case-control studies including 3,533 cases and 4,696 controls were eventually collected. Overall, we found that XRCC3 Thr241Met polymorphism was significantly associated with the risk of gliomas (T vs. C: OR=1.10, 95%CI=1.01-1.20, P=0.034; TT vs. CC: OR=1.30, 95%CI=1.03-1.65, P=0.027; TT vs. TC/CC: OR=1.29, 95%CI=1.01-1.64, P=0.039). In the subgroup analysis based on ethnicity, the significant association was found in Asian under four models (T vs. C: OR=1.17, 95%CI=1.07-1.28, P=0.00; TT vs. CC: OR=1.79, 95%CI=1.36-2.36, P=0.00; TT vs. TC/CC: OR=1.75, 95%CI=1.32-2.32, P=0.00; TT/TC vs. CC: OR=1.11,95% CI=1.02-1.20). This meta-analysis suggested that the XRCC3 Thr241Met polymorphism is a risk factor for gliomas, especially for Asians. Considering the limited sample size and ethnicities included in the meta-analysis, further large scale and well-designed studies are needed to confirm our results.