• Title/Summary/Keyword: clamp

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Modulation of Cyclic AMP-PKA System on Inhibitory and Excitatory Herbs-induced Ion Currents (Cyclic AMP-PKA System이 억제성 및 흥분성 한약재에 의해 유발된 이온전류에 미치는 영향)

  • Kim Youn-Jung;Shin Min-Chul;Chung Joo-Ho;Lee Choong-Yeol;Kim Ee-Hwa;Kim Chang-Ju
    • Korean Journal of Acupuncture
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    • v.18 no.1
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    • pp.143-156
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    • 2001
  • Modulatory effect of cAMP-PKA system on inhibitory- and excitatory herbs-induced ion currents were investigated by nystatin-perforated patch clamp method under voltage-clamp condition. Ion currents induced by Bupleuri radix and Coptidis rhizoma were not affected by cAMP-PKA system. Ion current induced by Ecliptae herba was partially inhibited by cAMP-PKA system. Ion currents induced by Aconiti tuber and Boshniakiae herba were inhibitory modulated and ion current induced by Zingiberis rhizoma was excitatory modulated by cAMP-PKA system. Modulation of cAMP-PKA system on ion currents induced by Ginseng radix was fluctuated. In this results, it can be seen generally that excitatory herbs-induced ion currents were modulated by cAMP-PKA system while cAMP-PKA system did not affect inhibitory herbs-induced ion currents.

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Effects of Hydrogen Peroxide on Neuronal Excitability and Synaptic Transmission in Rat Substantia Gelatinosa Neurons

  • Son, Yong;Chun, Sang-Woo
    • International Journal of Oral Biology
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    • v.32 no.4
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    • pp.153-160
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    • 2007
  • The superficial dorsal horn, particularly substantia gelatinosa (SG) in the spinal cord, receives inputs from small-diameter primary afferents that predominantly convey noxious sensation. Reactive oxygen species (ROS) are toxic agents that may be involved in various neurodegenerative diseases. Recent studies indicate that ROS are also involved in persistent pain through a spinal mechanism. In the present study, whole cell patch clamp recordings were carried out on SG neurons in spinal cord slice of young rats to investigate the effects of hydrogen peroxide on neuronal excitability and excitatory synaptic transmission. In current clamp condition, tert-buthyl hydroperoxide (t-BuOOH), an ROS donor, depolarized membrane potential of SG neurons and increased the neuronal firing frequencies evoked by depolarizing current pulses. When slices were pretreated with phenyl-N-tert-buthylnitrone (PBN) or ascorbate, ROS scavengers, t-BuOOH did not induce hyperexcitability. In voltage clamp condition, t-BuOOH increased the frequency and amplitude of spontaneous excitatory postsynaptic currents (sEPSCs), and monosynaptically evoked excitatory postsynaptic currents (eEPSCs) by electrical stimulation of the ipsilateral dorsal root. These data suggest that ROS generated by peripheral nerve injury can modulate the excitability of the SG neurons via pre- and postsynaptic actions.

Analysis of Leakage Current Diagnosis According to Online and Offline Conditions (On-Line 및 Off-Line 상태에 따른 누설 전류 진단 분석)

  • Han, Kyung-Chul;Lee, Gyeong-Seop;Choi, Yong-Sung
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.31 no.4
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    • pp.261-266
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    • 2018
  • When the clamp meter approaches the electric path where current is flowing, leakage current can be measured at a distance from the electric current because the induced current increases as the magnitude of the current increases and approaches nearer to the electric path. Therefore, measurements were carried out from a distance to avoid this effect. In addition, the measured values differ depending on the location of the power line that penetrates the ZCT of the clamp meter, thus measurements were performed at a location where this effect was minimized. The fraction of compliant branch circuits, whose leakage current was lower than 1.00 mA, was found to be 69.0% out of the total of 439 branch circuits, while the percentage of compliant branch circuits having an insulation resistance higher than $0.20M{\Omega}$ was found to be 93.2%. The reason why the percentage of compliant branch circuits with low leakage current was low might be due to the inclusion of capacitive leakage current in the total measured leakage current.

Single Power-conversion AC-DC Converter with High Power Factor (고역률을 갖는 단일 전력변환 AC-DC 컨버터)

  • Cho, Yong-Won;Park, Chun-Yoon;Kwon, Bong-Hwan
    • The Transactions of the Korean Institute of Power Electronics
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    • v.19 no.1
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    • pp.23-30
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    • 2014
  • This paper proposes a single power-conversion ac-dc converter with a dc-link capacitor-less and high power factor. The proposed converter is derived by integrating a full-bridge diode rectifier and a series-resonant active-clamp dc-dc converter. To obtain a high power factor without a power factor correction circuit, this paper proposes a suitable control algorithm for the proposed converter. The proposed converter provides single power-conversion by using the proposed control algorithm for both power factor correction and output control. Also, the active-clamp circuit clamps the surge voltage of switches and recycles the energy stored in the leakage inductance of the transformer. Moreover, it provides zero-voltage turn-on switching of the switches. Also, a series-resonant circuit of the output-voltage doubler removes the reverse-recovery problem of the output diodes. The proposed converter provides maximum power factor of 0.995 and maximum efficiency of 95.1% at the full-load. The operation principle of the converter is analyzed and verified. Experimental results for a 400W ac-dc converter at a constant switching frequency of 50kHz are obtained to show the performance of the proposed converter.

Analysis and Implementation of a New Single Switch, High Voltage Gain DC-DC Converter with a Wide CCM Operation Range and Reduced Components Voltage Stress

  • Honarjoo, Babak;Madani, Seyed M.;Niroomand, Mehdi;Adib, Ehsan
    • Journal of Power Electronics
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    • v.18 no.1
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    • pp.11-22
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    • 2018
  • This paper presents a single switch, high step-up, non-isolated dc-dc converter suitable for renewable energy applications. The proposed converter is composed of a coupled inductor, a passive clamp circuit, a switched capacitor and voltage lift circuits. The passive clamp recovers the leakage inductance energy of the coupled inductor and limits the voltage spike on the switch. The configuration of the passive clamp and switched capacitor circuit increases the voltage gain. A wide continuous conduction mode (CCM) operation range, a low turn ratio for the coupled inductor, low voltage stress on the switch, switch turn on under almost zero current switching (ZCS), low voltage stress on the diodes, leakage inductance energy recovery, high efficiency and a high voltage gain without a large duty cycle are the benefits of this converter. The steady state operation of the converter in the continuous conduction mode (CCM) and discontinuous conduction mode (DCM) is discussed and analyzed. A 200W prototype converter with a 28V input and a 380V output voltage is implemented and tested to verify the theoretical analysis.

Development of High Tension Tensile Tester for Transmission Line (송배전 선로 고장력 인장시험기 개발)

  • Shin, Dong-Hwa;Lee, Byung-Ho
    • Journal of the Korean Society of Industry Convergence
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    • v.21 no.5
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    • pp.219-225
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    • 2018
  • In this paper, for the testing of tensile strength of dead-end clamp used in transmission line, resulting values were estimated by designing and producing the horizontal version of widely-used vertical tensile tester. Tensile strength test of dead-end clamp for transmission line is essential for quality test of products. Moreover, tensile tester is an equipment that requires high level of reliability which needs to be examined through sampling tests commensurate with total inspection. Frames of tensile tester were made up of H-beams so that it can endure more than 20 [tons] of load capability and the test was implemented for 60[seconds] applying five types of tension. In consequence, the tester could withstand up to 21,600[kg] of weight as well as all types of tension. This newly developed horizontal tensile tester can be utilized in figuring out properties of various materials by estimating tensile strength of materials such as metal, rubber and fiber.

A Three-phase Current-fed DC-DC Converter with Active Clamp (연료전지용 3상 전류형 능동클램프 DC-DC 컨버터)

  • Cha, Han-Ju;Choi, Jung-Wan;Yoon, Gi-Gab
    • The Transactions of the Korean Institute of Power Electronics
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    • v.12 no.6
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    • pp.456-464
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    • 2007
  • This paper proposes a novel three-phase current-fed active clamp DC-DC converter for fuel cells. A single common active clamp branch is used to limit transient voltage across the three-phase full bridge and to realize zero-voltage switching(ZVS) in all switches. To apply for the power generation system current-fed type has been combined with the three-phase power conversion system. The proposed approach has the following advantages: an increase (by a factor of three) of input current and output voltage chopping frequencies; lower RMS current through the inverter switches with higher power transfer capability; reduction in size of reactive later components and the power conditioning system; better transformer utilization; increase of the system reliability. Therefore, the proposed three-phase current-fed active clamp DC-DC converter is appropriate for the boost type DC-DC converter for fuel cells and also applicable for the photovoltaic and battery charge system. The paper details the analysis, simulation and hardware implementation of the proposed system. Finally, experimental results with the proposed PWM strategy demonstrate the feasibility of the proposed scheme on a 500W prototype converter.

Effects of Incompatibility on Protoplast Fusion between intra-and inter Species in Basidiomycete, Pleurotus spp. (느타리버섯의 불화합성(不和合性)이 종내(種內) 및 종간(種間) 원형질체(原形質體) 융합(融合)에 미치는 영향(影響))

  • Go, Seung-Joo;You, Chang-Hyun;Shin, Gwan-Chull
    • The Korean Journal of Mycology
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    • v.17 no.3
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    • pp.137-144
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    • 1989
  • Effects of incompatibility existing between intra-and interspecies in Pleurotus spp. on protoplast fusion, clamp formation of their fusants and fruitbody production were investigated. Protoplast fusion between intra-and interspecies of the fungus was achieved by Poly ethylene glycol treatment. The fusion frequency between intraspecies was a little higher than that of interspecies. Fusion frequency between interspecies was not correlated with their similarities based on isozyme patterns. In case of protoplast fusion between intra-and interspecies, the fusants from the compatible isolates produced normal fruit bodies, while those from the incompatible isolates did not produce clamp connections and fruit bodies except those of a few isolates presumed mutants.

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Strain improvement of Pleurotus ostreatus using self-fertility monospore isolate (느타리 단핵 임성체를 이용한 균주개발)

  • Yoo, Young-Bok;Kim, In-Yeup;Kong, Won-Sik;Jang, Kab-Yeul;Oh, Se-Jong;Jhune, Chang-Sung
    • Journal of Mushroom
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    • v.4 no.2
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    • pp.48-52
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    • 2006
  • Oyster mushroom, Pleurotus ostreatus is bifactorial heterothallism. Single basidiospore isolates from fruiting bodies are homokaryotic and self-sterile. However, we found that homokaryons derived from some strains of P. ostreatus could develop fruiting bodies with two different types. One hundred and two isolates out of 155 monospore isolates formed fruiting bodies (65.8%). First group did not have only mature or sporulating fruiting bodies but also clamp connections, which initial isolate also did not present clamp connections (Abortive homokaryotic fruiting, AHF). Second group had developed fruiting bodies with clamp connections even though initial homokaryotic colony did not form clamp connections (Pseudo- homokaryotic fruiting, PHF). The mycelial colonies derived from PHF by tissue culture formed clamp connections, while mycelial colonies of AHF lacked them. We obtained 535 PHF and 79 AHF inter-strain hybrids among 8 strains of P. ostreatus by hyphal anastomosis. The fruiting body yield of PHF group is higher than that of AHF group in bottle cultivation. A preselection of single spore isolates for fertility would save labour in strain improvement of P. ostreatus.

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A Experimental Analysis on the Relationship between the Angle of a Locker and the Clamping Force of Wedge Type Rail Clamp (Locker의 물림각과 쐐기형 레일클램프의 압착력에 관한 실험적 고찰)

  • Han Dong-Seop;Shim Jae- Joon;Han Geun- Jo;Lee Kwon-Hee
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2005.10a
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    • pp.247-252
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    • 2005
  • The wedge type rail clamp has the operating mechanism: First, the jaw pad clamps a rail with small clamping force Next as the wind speed increases, the clamping force of the jaw pad is increased by the wedge. In order to design the wedge type rail clamp, we need to determine the proper wedge angle to minimize the sliding distance of a roller and the proper clamping angle of a locker to generate the initial clamping force of a jaw pad. The researches for the proper wedge angle have conducted, and in this study we conducted the investigation to determine the proper clamping angle of a locker in the rail clamp with wedge angle of $10^{\circ}$. Because the initial damping force of the jaw pad was determined by the clamping angle of the locker, in order to carry out the clamping force of a jaw pad, we measured the locking force applied to a locker with respect to the clamping angle of a locker, such as $3^{\circ},\;4^{\circ},\;5^{\circ},\;6^{\circ},\;$ using a pressure gauge, and compared the results with the FEA results.

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