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Optimization in Extraction Conditions of Carotenoids from Citrus unshiu Press Cake by Supercritical Carbon Dioxide  

Lim, Sang-Bin (Department of Food Science and Engineering, Cheju National University)
Jwa, Mi-Kyung (Department of Food Science and Engineering, Cheju National University)
Publication Information
Korean Journal of Food Science and Technology / v.35, no.6, 2003 , pp. 1104-1109 More about this Journal
Abstract
Response surface methodology (RSM) was used to investigate the effects of the processing parameters on supercritical $CO_2\;(SC-CO_2)$. extraction of total carotenoids and ${\beta}$-cyptoxanthin from Citrus unshiu press cake. The parameters tested were $SC-CO_2$ pressure, dynamic extraction time, and concentration of ethanol added as the modifier to $CO_2$. Experimental data correlated well with the processing parameters (p<0.01), and there was a high statistically significant multiple regression relationship for the extraction of total carotenoids and ${\beta}-cyrptoxanthin$ ($R^2=0.9789$ and 0.9796, respectively). The optimal processing conditions were extraction pressure 33.4 and 37.3 MPa, extraction time 39.6 and 41.0 min, ethanol concentration 18.6 and 17.0% for total carotenoids and ${\beta}-cryptozanthin$, respectively. Maximum extraction yields predicted by RSM were 61.1 and 95.8% ppm, respectively. The extraction yield of total carotenoids increased asymptotically with the increase of the extraction pressure. It increased in proportion to extraction time and concentration of the cosolvent. The extraction yield of ${\beta}-cryptoxanthin$ increased with extraction pressure, extraction time, and concentration of the cosolvent. The extraction time and the concentration of the cosolvent, and the interaction between extraction time and the concentration of the cosolvent significantly affected the extraction yields of carotenoids from C. unshiu press cake.
Keywords
Citrus unshiu press cake; supercritical carbon dioxide extraction; carotenoids; ${\beta}-cryptoxanthin$; response surface methodology;
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1 Wingerath, T., Stahl, W. and Sies, H. $\beta$-Cryptoxanthin selectively increases in human chylomicrons upon ingestion of tangerin concentrate rich in $\beta$-cryptoxanthin esters. Arch. Biochem. Biophy. 324: 385-390 (1995)   DOI   ScienceOn
2 Vega, P.J., Balaban, M.O., Sims, C.A., O'Keefe, S.F. and Cornell, J.A. Supercritical carbon dioxide extraction efficiency for carotenes from carrots by RSM. J. Food Sci. 61: 757-765 (1996)   DOI   ScienceOn
3 Wattenberg, L.W. Inhibition of neoplasia by minor dietary constituents. Cancer Res. 43: 485-535 (1993)
4 Schmitz, H.H., Schwartz, S.J. and Catignani, G.L. Resolution and quantitation of the predominant geometric $\beta$-carotene isomers present in human serum using normal-phase HPLC. J. Agric. Food Chem. 42: 2746-2750 (1994)   DOI   ScienceOn
5 SAS Institute, Inc. SAS User's Guide. Statistical Analysis Systems Institute, Cary, NC, USA (1996)
6 Lim, S., Jung, S.K. and Jwa, M.K. Extraction of valuable substances from Citrus peel by supercritical carbon dioxide. Food Sci. Biotechnol. 11: 644-648 (2002)
7 Myers, R.H. and Montgomery, D.C. Response Surface Methodology, pp. 208-350. John Wiley & Sons Inc., NY,USA (1995)
8 Barth, M.M., Zohu, C, Kute, K.M. and Rosenthal, G.A. Determination of optimum conditions for supercritical fluid extraction of carotenoids from carrot tissue. J. Agric. Food Chem. 43: 2876-2878 (1995)   DOI
9 Nishino, R., Tokuda, R., Satomi, Y., Masuda, M., Bu, P., Ono zuka, M., Yamaguchi, S., Okuda, Y., Takayasu, J., Tsuruta, J., Okuda, M., Ichiishi, E., Murakoshi, M., Kato, T., Misawa, N., Narisawa, T., Takasuka, N. and Yano, M. Cancer prevention by carotenoids. Pure Appl. Chem. 71: 2273-2278 (1999)   DOI   ScienceOn
10 Wingerath, T., Stahl, W., Kirsch, D., Kaufmann, R. and Sies, H. Fruit juice carotenol fatty acid esters and carotenoids as identified by matrix-assisted laser desorption ionization mass spectrometry. J. Agric. Food Chem. 44: 2006-2013 (1996)   DOI   ScienceOn
11 Riso, P. and Porrini, M. Determination of carotenoids in vegetable foods and plasma. Internal. J. Vit. Nutr. Res. 67: 47-54 (1996)
12 Hawthorne, S.B. and Miller, D.J. Direct comparison of soxhlet and low- and high-temperature supercritical CO_2 extraction efficiencies of organics from environmental solids. Anal. Chem. 66: 4005-4012 (1994)   DOI   ScienceOn
13 Block, G., Patterson, B. and Subar, A. Fruit, vegetables, and cancer prevention: a review of epidemiological evidence. Nutr. Cancer 18: 1-29 (1992)   DOI   PUBMED   ScienceOn
14 Steinmetz, K.A. and Potter, J.D. Food-group consumption and colon cancer in the Adelaide case-control study. Int. J. Cancer 53: 711-719 (1993)   DOI   ScienceOn
15 Lim, S., Jung, S.K. and Jwa, M.K. Extraction of carotenoids from Citrus unshiu press cake by supercritical carbon dioxide. Food Sci. Biotechnol. 12: 513-520 (2003)
16 Burton, G.W. and Ingold, K.U. $\beta$-Carotene: an unusual type of lipid antioxidant. Science 224: 569-572 (1985)   DOI
17 Langenfeld, J.J., Hawthorne, S.B., Miller, D.J. and Pawliszyn, J. Role of modifiers for analytical-scale supercritical fluid extraction of environmental samples. Anal. Chem. 66: 909-916 (1994)   DOI   ScienceOn
18 Hart, D.J. and Scott, K.J. Development and evaluation of an HPLC method for the analysis of carotenoids in foods, and the measurement of the carotenoid content of vegetables and fruits commonly consumed in the UK. Food Chem. 54: 101-111 (1995)   DOI   ScienceOn
19 Baysal, T., Ersus, S. and Starmans, D.A.J. Supercritical CO_2 extraction of $\beta$-carotene and lycopene from tomato paste waste. J. Agric. Food Chem. 48: 5507-5511 (2000)   DOI   ScienceOn
20 Turner, C., King, J.W. and Mathiasson, L. Supercritical fluid extraction and chromatography for fat-soluble vitamin analysis. J. Chromatogr. A. 936: 215-237 (2001)   DOI   PUBMED   ScienceOn