• Journal of the Korean Society for Nondestructive Testing
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• v.26 no.2
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• pp.108-114
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• 2006

Transient and asymmetric density distributions of butane flow have been investigated from laser image signals by developed three-dimensional digital speckle tomography. Moved signals of speckles have been captured by multiple CCD images in three angles of view simultaneously because the flows were asymmetric and transient. The signals of speckle movements between no flow and downward butane flow from a circular half opening have been calculated by a cross-correlation tracking method so that those distances can be transferred to deflection angles of laser rays fur density gradients. The three-dimensional density fields have been reconstructed from the fringe shift signal which is integrated from the deflection angle by a real-time multiplicative algebraic reconstruction technique (MART).

• Publications of The Korean Astronomical Society
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• v.30 no.2
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• pp.595-597
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• 2015

LMC X-4 is an eclipsing high-mass X-ray binary exhibiting a superorbital modulation with a period of ~ 30:5 days. We present a detailed study of the variations of the superorbital modulation period with a time baseline of ~ 18 years. The period determined in the light curve collected by the Monitor of All-sky X-ray Image (MAXI) significantly deviates from that observed by the All Sky Monitor (ASM) onboard the Rossi X-ray Timing Explorer (RXTE). Using the data collected by RXTE/ASM, MAXI, and the Burst Alert Telescope (BAT) onboard Swift, we found a significant period derivative, $\dot{P}=(2.08{\pm}0.12){\times}10^{-5}$. Furthermore, the O{C residual shows complex short-term variations indicating that the superorbital modulation of LMC X-4 exhibits complicated unstable behaviors. In addition, we used archive data collected by the Proportional Counter Array (PCA) on RXTE to estimate the orbital and spin parameters. The detected pulse frequencies obtained in small time segments were fitted with a circular orbital Doppler shift model. In addition to orbital parameters and spin frequency for each observation, we found a spin frequency derivative of $\dot{v}=(6.482{\pm}0.011){\times}10^{-13}Hz{\cdot}s^{-1}$. More precise orbital and spin parameters will be evaluated by the pulse arrival time delay technique in the future.

• BMB Reports
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• v.40 no.3
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• pp.315-324
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• 2007

The novel $\alpha$-amylase purified from locally isolated strain, Bacillus sp. KR-8104, (KRA) (Enzyme Microb Technol; 2005; 36: 666-671) is active in a wide range of pH. The enzyme maximum activity is at pH 4.0 and it retains 90% of activity at pH 3.5. The irreversible thermoinactivation patterns of KRA and the enzyme activity are not changed in the presence and absence of $Ca^{2+}$ and EDTA. Therefore, KRA acts as a $Ca^{2+}$-independent enzyme. Based on circular dichroism (CD) data from thermal unfolding of the enzyme recorded at 222 nm, addition of $Ca^{2+}$ and EDTA similar to its irreversible thermoinactivation, does not influence the thermal denaturation of the enzyme and its Tm. The amino acid sequence of KRA was obtained from the nucleotide sequencing of PCR products of encoding gene. The deduced amino acid sequence of the enzyme revealed a very high sequence homology to Bacillus amyloliquefaciens (BAA) (85% identity, 90% similarity) and Bacillus licheniformis $\alpha$-amylases (BLA) (81% identity, 88% similarity). To elucidate and understand these characteristics of the $\alpha$-amylase, a model of 3D structure of KRA was constructed using the crystal structure of the mutant of BLA as the platform and refined with a molecular dynamics (MD) simulation program. Interestingly enough, there is only one amino acid substitution for KRA in comparison with BLA and BAA in the region involved in the calcium-binding sites. On the other hand, there are many amino acid differences between BLA and KRA at the interface of A and B domains and around the metal triad and active site area. These alterations could have a role in stabilizing the native structure of the loop in the active site cleft and maintenance and stabilization of the putative metal triad-binding site. The amino acid differences at the active site cleft and around the catalytic residues might affect their pKa values and consequently shift its pH profile. In addition, the intrinsic fluorescence intensity of the enzyme at 350 nm does not show considerable change at pH 3.5-7.0.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.40 no.3
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• pp.173-179
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• 2016

This is a numerical study on the curved channel type of hydrogen reformer using the commercial code of fluid dynamics. We numerically compared the numerical model in a previous study model and the modelling of a tube type curved channel. In the result of numerical analysis on 4 types of curved channel reformers, the methanol conversion efficiency of type 1~4 were 45.0%, 45.3%, 45.6%, 45.6% respectively, and there was hardly any difference by ${\pm}0.6%$. In light of flow characteristics, the rectangle type tube and the type 2 with $45^{\circ}$ turn showed most uniform flow characteristics and concentration distribution of methanol, and the circular type tube and the type 3 with $90^{\circ}$ turn had most un-uniform flow characteristics and concentration distribution of methanol. We concluded that the design for curved channel reformer has to have rectangle type tube with curve of almost $45^{\circ}$ as in the type of curved pipe with $45^{\circ}$ turn.

• Korean Journal of Optics and Photonics
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• v.29 no.4
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• pp.166-172
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• 2018

In this paper, we propose a new method to predict a mirror's surface deformation due to the stress of moisture release by a coating in the environment of outer space. We measured the surface deformation of circular samples 50 mm in diameter and 1.03 mm thick, using an interferometer. The results were analyzed using Zernike fringe polynomials. The coating stress caused by moisture release was calculated to be 152.7 MPa. This value was applied to an analytic model of a 1.25 mm thickness sample mirror, confirming that the change of surface deformation could be predicted within the standard deviation of the measurement result ($78.9{\pm}5.9nm$). Using this methodology, we predicted the surface deformation of 600 mm hyperbolic mirror for the Compact Advanced Satellite, which will be launched in 2019. The result is only $2.005{\mu}m$ of focal shift, leading to 2.3% degradation of modulation transfer function (MTF) at the Nyquist frequency, which satisfies the requirement.

• Analytical Science and Technology
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• v.13 no.4
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• pp.494-503
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• 2000

A 21-residue peptide corresponding to amino acids 84-104 of $p16^{INK4A}$, the tumor suppressor, has been synthesized and its structure was studied by Circular Dichroism, $^1H$ NMR spectroscopy and molecular modeling. A p16-derived peptide (84-104 amino acids) forming stable complex with CDK4 and CDK6 inhibits the ability of CDK4/6 to phosphorylate pRb in vitro, and blocks cell-cycle progression through G1/S phase as shown in the function of the full-length p16. Its NMR spectral data including NOEs, $^3J_{NH-H{\alpha}}$ coupling constants, $C_{\alpha}H$ chemical shift, the average amplitude of amide chemical shift oscillation and temperature coefficients indicate that the secondary structure of a p16-derived peptide is similar to that of the same region of full-length p16, which consists of helix-turn-helix structure. The 3-D distance geometry structure based on NOE-hased distance and torsion angle restraints is characterized by ${\gamma}$-turn conformation between residues $Gly^{89}-Leu^{91}$(${\varphi}_{i+1}=-79.8^{\circ}$, ${\varphi}_{i+1}=60.2^{\circ}$) as evidenced in a single crystal structure for the corresponding region of p18 or p19, but is undefined at both the N and C termini. This compact and rigid ${\gamma}$-turn region is considered to stabilize the structure of p16-derived peptide and serve as a site recognizing cyelin dependent kinase, and this well-defined ${\gamma}$-turn structure could be utilized for the design of anti-cancer drug candidates.

• Economic and Environmental Geology
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• v.55 no.5
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• pp.551-561
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• 2022

Geophysical exploration methods are very useful for generating high-resolution images of underground structures, and such methods can be applied to investigation of buried cultural properties and for determining their exact locations. In this study, image feature extraction and image segmentation methods were applied to automatically distinguish the structures of buried relics from the high-resolution ground-penetrating radar (GPR) images obtained at the center of Silla Kingdom, Gyeongju, South Korea. The major purpose for image feature extraction analyses is identifying the circular features from building remains and the linear features from ancient roads and fences. Feature extraction is implemented by applying the Canny edge detection and Hough transform algorithms. We applied the Hough transforms to the edge image resulted from the Canny algorithm in order to determine the locations the target features. However, the Hough transform requires different parameter settings for each survey sector. As for image segmentation, we applied the connected element labeling algorithm and object-based image analysis using Orfeo Toolbox (OTB) in QGIS. The connected components labeled image shows the signals associated with the target buried relics are effectively connected and labeled. However, we often find multiple labels are assigned to a single structure on the given GPR data. Object-based image analysis was conducted by using a Large-Scale Mean-Shift (LSMS) image segmentation. In this analysis, a vector layer containing pixel values for each segmented polygon was estimated first and then used to build a train-validation dataset by assigning the polygons to one class associated with the buried relics and another class for the background field. With the Random Forest Classifier, we find that the polygons on the LSMS image segmentation layer can be successfully classified into the polygons of the buried relics and those of the background. Thus, we propose that these automatic classification methods applied to the GPR images of buried cultural heritage in this study can be useful to obtain consistent analyses results for planning excavation processes.