• Bulletin of the Korean Chemical Society
• /
• v.18 no.5
• /
• pp.510-514
• /
• 1997

We studied the interaction of 4',6-diamidino-2-phenylindole (DAPI) with single stranded poly(dA) using optical spectroscopic methods, including absorption, circular dichroism (CD), and fluorescence spectroscopy. The temperature-dependent conformation of poly(dA) was also investigated. The conformation of poly(dA) varied with temperature, which is explained by the stacking-destacking process of the adenine bases, resulting from the sugar conformation. The hypochromicity and red-shift in the absorption spectroscopy, the lack of CD change in the drag absorption region, and the fluorescence behavior, especially a great accessibility of the I2 quencher to the poly(dA)-bound DAPI, suggest that DAPI binds to the outside of poly(dA). The Job plot for the DAPI-poly(dA) mixture demonstrated that a stoichiometry of one DAPI molecule binds to the one phosphate of poly(dA).

• Bulletin of the Korean Chemical Society
• /
• v.17 no.4
• /
• pp.358-362
• /
• 1996

The ionic strength dependent binding mode of 9-aminoacridine (9AA), a well-known DNA intercalator, to DNA is studied by flow linear dichroism, circular dichroism, fluorescence techniques and equilibrium dialysis. The DNA-bound 9AA exhibits spectral properties corresponding to the intercalative binding mode disregarding the salt concentrations; the angle between the long-axis transition moment of the 9AA molecule and DNA helix axis is calculated to be about 65°, indicating a significant deviation from the classical intercalation. At low salt concentrations, however, upwards bending curve in Stern-Volmer plot is observed (where 9AA is a fluorophore and DNA a quencher), indicating the coexistence of both static and dynamic quenching mechanisms or the existence of an additional binding site.

• The Korean Journal of Quaternary Research
• /
• v.17 no.2
• /
• pp.163-163
• /
• 2003

The study area, Kwangneung Experiment Forest (KEF) is located on the west-central portion of Korean peninsula and belongs to a cool-temperate broadleaved forest zone. At the old-growth deciduous forest near Soribong-peak (533.1m) in KEF, we have established a permanent plot and a flux tower, and the site was registered as a KLTER site and also a KoFlux site. In this study, we aimed to present basic ecological characteristics and synthetic data of carbon budgets and flows, and some monitoring data which are essential for providing important parameters and validation data for the forest dynamics models or biogeochemical dynamics models to predict or interpolate spatially the changes in forest ecosystem structure and function. We made a stemmap of trees in 1 ha plot and analyzed forest stand structure and physical and chemical soil characteristics, and estimated carbon budgets by forest components (tree biomass, soils, litter and so on). Dominant tree species were Quercus serrata and Carpinus laxiflora, and accompanied by Q. aliena, Carpinus cordata, and so on. As a result of a field survey of the plot, density of the trees larger than 2cm in DBH was 1,473 trees per ha, total biomass 261.2 tons/ha, and basal area 28.0 m2/ha. Parent rock type is granite gneiss. Soil type is brown forest soil (alfisols in USDA system), and the depth is from 38 to 66cm. Soil texture is loam or sandy loam, and its pH was from 4.2 to 5.0 in the surface layer, and from 4.8 to 5.2 in the subsurface layer. Seasonal changes in LAI were measured by hemispherical photography at the l.2m height, and the maximum was 3.65. And the spatial distributions of volumetric soil moisture contents and LAIs of the plot were measured. Litterfall was collected in circular littertraps (collecting area： 0.25m2) and mass loss rates and nutrient release patterns in decomposing litter were estimated using the litterbag technique employing 30cm30cm nylon bags with l.5mm mesh size. Total annual litterfall was 5,627 kg/ha/year and leaf litter accounted for 61% of the litterfall. The leaf litter quantity was highest in Quercus serrata, followed by Carpinus laxiflora and C. cordata, etc. Mass loss from decomposing leaf litter was more rapid in C. laxiflora and C. cordata than in Q. serrata litter. About 77% of C. laxiflora and 84% of C. cordata litter disappeared, while about 48% in Q. serrata litter lost over two years. The carbon pool in living tree biomass including below ground biomass was 136 tons C/ha, and 5.6 tons C/ha is stored in the litter layer, and about 92.0 tons C/ha in the soil to the 30cm in depth. Totally more than about 233.6 tons C/ha was stored in DK site. And then we have drawn a schematic diagram of carbon budgets and flows in each compartment of the KEF site.

• Journal of Korean Society of Forest Science
• /
• v.95 no.5
• /
• pp.621-627
• /
• 2006

This study was conducted to understand the pattern and characteristics of seed rain in the broadleaved-Korean pine mixed forest. We established 287 $0.5m^2$ circular seed traps and collected and identified fallen seeds in the traps every two weeks in $150m{\times}150m$ sample plot of the permanent nine hectare of experimental plot in 2005. The overall average density of seed rain was $864.2/m^2$. The seeds of Betula costata Betula costata had the highest number of fallen seeds as $676.0/m^2$ (78.2%), followed by Abies nephrolepis as $57.5/m^2$, B. platyphylla as $37.9/m^2$, Tilia amurensis as $32.2/m^2$, Acer ukurunduense as $17.0/m^2$, A. tegmentosum $14.8/m^2$, and so on. Pinus koraiensis was recorded only $2.5/m^2$ of fallen seeds mainly owing of Korean pine had low rate of purity due to the animal and microbiological predation. Most of seed dispersal have started from the middle to late August and come to an end on the middle of November. The peak time of seed dispersal varied depending on the species. The rate of pure seed by dispersal time varied according to the species, thereupon the aspect of predation and the rate of blasted seed which had influence on the rate of purity also varied according to the species. The density of Korean pine seed rain in the forest gap was significantly different at $P{\leq}0.05$ from in the closed canopy. But the other species had no difference among canopy coverage.

• Journal of the Korean Magnetic Resonance Society
• /
• v.9 no.1
• /
• pp.38-47
• /
• 2005

Axin is a scaffold protein of the APC/axin/GSK complex, binding to all of the other signalling components. Axin interacts with Glycogen synthase kinase 3$\beta$ (GSK 3$\beta$) and functions as a negative regulator of Wnt signalling pathways. To determine the solution structure of the GSK3$\beta$ binding regions of the axin, we initiated NMR study of axin fragment comprising residues 3$Val^{388} - Arg^{401}$using circular dichroism (CD) and two-dimensional NMR spectroscopy. The CD spectra of 3$axin^{pep}$ in the presence of 30% TFE displayed a standard 3$\alpha$-helical conformation, exhibiting the bound structure of 3$axin^{pep}$ to GSK3$\bata$. On the basis of experimental restraints including $NOE_s$, and $^3J_{HN\alpha} $ coupling constants, the solution conformation of $axin^{pep}$ was determined with program CNS. The 20 lowest energy structures were selected out of 50 final simulated-annealing structures in both water and TFE environment, respectively. The $RMSD_s$ for the 20 structures in TFE solution were 0.086 nm for backbone atoms and 0.195 nm for all heavy atoms, respectively. The Ramachandran plot indicates that the $\varphi$, $\psi$ angles of the 20 final structures is properly distributed in energetically acceptable regions. $Axin^pep$ in aqueous solutions consists of a stable $\alpha$-helix spanning residues form $Glu^{391}$ to $Val^{391} $, which is an interacting motif with GSK3$\beta$.

• Journal of Ecology and Environment
• /
• v.33 no.4
• /
• pp.363-376
• /
• 2010

Vegetative communities of created wetlands often display lower species richness, less cover, higher occurrence of non-native or invasive species, and fewer obligate wetlands species than those in natural wetlands, thus failing to meet basic success criteria for wetland mitigation. This study examined the effects of two design elements, disking-induced microtopography and hydrologic regime, on the first year vegetation development pattern of a mitigation wetland newly created in the Virginia piedmont. Elevation and species cover were measured along replicate multiscale circular transects in two adjacent wetland sites that are different in their hydrologic regime. Two microtopographic indices, tortuosity (T) and limiting elevation difference (LD), were calculated from the elevation measurements. Both indices were higher in disked plots than non-disked plots, showing the effect of disking on microtopography. Out of forty-one vegetation taxa observed in the wetland, 29 taxa were naturally colonized and 12 taxa were seeded. All plots except one non-disked plot were dominated by wetland vegetation. Species richness and diversity were higher in disked than in non-disked plots. Vegetation community development seemed also influenced significantly by hydrologic regime of the site. The effect of microtopography on species richness and diversity was more pronounced in a relatively dry site compared to a wet site. In addition, percent cover, species richness and diversity of vegetation were positively correlated with microtopographic indices such as T and LD. Two design elements, microtopography and hydrologic regime, should be considered and incorporated in wetland creation to enhance plant community development.

• Journal of Forest and Environmental Science
• /
• v.32 no.1
• /
• pp.10-19
• /
• 2016

This study was carried out in the Boin and Miryousef regions in Baneh of Kordestan province, west of Iran. Both areas were divided into two parts including protected and unprotected areas. In each area, 20 $1000-m^2$ circular plots were established following a selective method. Tree and shrub species were identified and the number of these species was counted in each sampling plot. The results indicated that 7 trees, 4 shrubs and 109 herbaceous species were found in the studied areas, 40 herbaceous, 7 tree and 3 shrub species were present in protected area, while unprotected area had 16 herbaceous and 3 tree species. The highest value of diversity indices belonged to protected areas in all vegetation layers. In tree layer, Quercus brantii had the greatest value of SIV, whereas the lowest value belonged to Pistacia atlantica. In shrub layer, the highest and lowest values of SIV belonged to Daphne mezereum and Cerasus microcarpa, respectively. Finally, in herbaceous layer, Luzula spicata had highest value of SIV, and lowest value was belonged to Galium sp.

• Journal of Korean Society of Forest Science
• /
• v.97 no.2
• /
• pp.165-170
• /
• 2008

Litterfall has been recognized an important part of the forest ecosystem production, playing a major pathway in energy flow and nutrient cycling through the ecosystem. This study was carried out to examine the quantity and components, temporal variation, and spatial heterogeneity of the litterfall in the mixed broadleaved-Korean pine forest. The data were collected from the 9ha permanent experimental plot, of which on the center area, i.e. $150m{\times}150m$, the total number of 319 circular litterfall traps with the size of $0.5m^2$ were established to collect falling litterfall. The results showed that the annual amount of litterfall was totalized 3,033.7 kg/ha, occupying broad-leaves of 39.3%, conifer-leaves of 29.5%, others of 18.5%, branches of 10.4%, and seeds of 2.3%. The peak point of the litterfall production was made at the end of September, proportionating 32.2% of total amount. The analysis of semivariogram revealed the existence of high spatial heterogeneity, calculated the scale of spatial heterogeneity ranged from 11.6 m to 29.1 m. The result of proportion (C/[Co+C]) showed that spatial heterogeneity of autocorrelation in total spatial heterogeneity were from 97.0% to 100%. The relatively heavy branches and others had significant differences in litterfall production between the areas of canopy gap and closed canopy in the 95% probability level, but the other components did not show statistical differences.

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.36 no.3
• /
• pp.269-276
• /
• 2012

An air core is generated during draining through an axisymmetrically placed circular orifice after rotating a cylindrical tank filled with a liquid. If an air core is generated, the draining flow rate decreases and the draining time increases. In this study, the process of the formation of the air core and internal flow characteristics in a cylindrical tank are studied by numerical methods. Several methods are used in the analysis, and the results are compared with experimental results to obtain the appropriate scheme. Axial, radial, and swirl velocity profiles on a variety of heights are shown graphically, and the internal flow structure is analyzed from the velocity profiles, the vector plot, and the stream function distribution.

• Journal of Korean Society of Water and Wastewater
• /
• v.30 no.3
• /
• pp.253-261
• /
• 2016

A variety of methods for detecting the location of an underground water pipeline are being used across the world; the current main methods used in South Korea, however, have the problems of low precision and efficiency and the limitations in actual application. On this, this study developed locator capable of detecting the location of a water pipe by the use of an IMU sensor, and technology for using the extended karman filter to correct error in location detection and to plot the location on the coordinate system. This study carried out a tract test and a road test as basic experiments to measure the performance of the developed technology and equipment. As a result of the straight line, circular and ellipse track tests, the 1750 IMU sensor showed the average error of 0.08-0.11%; and thus it was found that the developed locator can detect a location precisely. As a result of the 859.6-m road test, it was found that the error was 0.31 m in case the moving rate of the sensor was 0.3-0.6 m/s; and thus it was judged that the equipment developed by this study can be applied to long-distance water pipes of over 1 km sufficiently. It is planned to evaluate its field applicability in the future through an actual pipe network pilot test, and it is expected that locator capable of detecting the location of a water pipe more precisely will be developed through research for the enhancement of accuracy in the algorithm of location detection.