• Korean Journal of Food Science and Technology
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• v.38 no.2
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• pp.159-164
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• 2006

Volatile components of Phellinus linteus produced from different areas were collected by simultaneous steam distillation-solvent extraction method (SDE). Concentrated extracts analyzed and identified by GC and GC-MS showed musty and earthy characteristics. 2-Methylphenol, methoxy benzene, coumaran, azulene, ${\alpha}-cedrene,\;{\alpha}-longipinene,\;{\beta}-selinene,\;{\alpha}-selinene$, camphor, ${\gamma}-ionone,\;{\beta}-ionone$, phytol, and borneol not reported in other edible mushrooms, were identified and/or tentatively identified in P. linteus for the first time. Main volatile components of P. linteus (Busan-Jinsung: BJ) were phytol from chlorophyll and methoxy benzenes having musty odor. Volatile components of P. linteus (Jinju-Kumwhang: JK) resembled those of BJ, but with high concentration of phenylacetaldehyde contributing to flower-odor. P. linteus (Cheju-Gullim: CG) contained low concentration of methoxy benzenes, but high concentration of phenylacetaldehyde. Low concentrations of ${\gamma}-ionone\;and\;{\beta}-ionone$ were identified in three kinds of P. linteus. They appeared to have been produced from degradation of carotenoid, which suggests P. linteus contains a carotenoid pigment.

• Korean Journal of Environmental Biology
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• v.24 no.1 s.61
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• pp.81-88
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• 2006

The cell abundance and marker pigment distribution patterns of picophytoplankton in the Chuuk Lagoon, tropical South Pacific, were analyzed flow cytometry and HPLC. Also, respective contribution of Synechococcus, Prochlorococcus and picoeukaryotes on estimated carbon biomass was evaluated. Synechococcus and Prochlorococcus showed contrasting distributional patterns in the waters of Chuuk Lagoon. Relatively high concentration of Synechococcus was observed near Weno Island but the concentration decreased toward the Northeast Passage. However, Prochlorococcus showed an opposite distributional pattern. Picoeukaryotes did not show any significant variable difference. The range of divinyl chlorophyll a (Chl. $\alpha$) concentration, marker pigment of Prochlorococcus, was $1.2\sim180.3\;ng\;L^{-1}$ and higher concentrations were observed at the stations near the Northeast Passage than stations near Weno Island. This pigment pattern was similar to cell abundance pattern indicating that chi. a2 may be a useful biomass indicator. On the other hand, the range of zeaxanthin concentrations was $61.4\sim135.8\;ng\;L^{-1}$ showing comparatively less significant variation indicating zeaxanthin influence derived from Prochlorococcus. Estimated carbon biomass of Synechococcus contributed 68% of total picophytoplankton biomass. Prochlorococcus and picoeukaryotes respectively contributed 17.1% and 14.9% of total picophytoplankton biomass.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.3
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• pp.369-385
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• 1996

The eco-hydrodynamic model was used to estimate the primary productivity of the oyster culture grounds in Kamak Bay. It is composed of the three-dimensional hydrodynamic model for the simulation of water flow and ecosystem model for the simulation of phytoplankton. The ecosystem model was applied to simulate phytoplankton biomass during culturing period in condition of no oyster culture grounds. The field surveys were conducted from May, 1994 to March, 1995 in Kamak bay. The results showed the concentration of chlorophyll $\alpha$ to be $1.00\~23.28\;{\mu}g/l$ in the surface layer, $1.27\~29.97\;{\mu}g/l$ in the middle layer and $1.23\~23.08\;{\mu}g/l$ the bottom layer. In monthly variations of chlorophyll $\alpha$ concentration, very high concentration were found in July, 1994 and very low concentrations in December, 1994. As the results of three-dimensional hydrodynamic simulation, the computed tidal currents ave mainly toward the inner part of bay through Yeosu Harbor and the southern mouth of a bay during the flood tide. The computed residual currents were dominated southward in Yeosu Harbor and eastward in the mouth of bay and also showed strong clockwise water circulation at the mouth of bay. The pattern between the simulated and observed tidal ellipses at three stations was very similar. The mean relative errors of all levels between the simulated and observed phytoplankton biomass at 14 stations in Kamak Bay were $13.81\%,\;9.31\%\;and\;17.84\%$, respectively. The results of phytoplankton biomass simulation showed that the biomass increased from June to September and rapidly decreased to December and then slowly increased to March. Primary productivity was estimated in the range of $0.99\~10.20gC/m^2/d$ with the average value of $4.43gC/m^2/d$ in condition of no oyster culture grounds. Primary productivity was rapidly increased from lune to August and rapidly decreased to December and then slowly increased from January to March in Kamak Bay.

• Applied Biological Chemistry
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• v.21 no.2
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• pp.84-102
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• 1978

Barley embryoless half seeds were incubated in medium containing $10{\mu}M$ GA. Time course activity changes of ${\alpha}-amylase$ were studied in extract and medium seperately by the addition of $0.1{\mu}M,\;5{\mu}M,\;and\;10{\mu}M$ ABA in midcourse incubation of 10 hours after GA treatment. MAK profiles of nucleic acids in embryoless half seeds were compared either with $10{\mu}M$ GA treatment or concomitant treatment with $10{\mu}M$ GA and $10{\mu}M$ ABA after 10 hours incubation, Time course changes of weight increase, chlorophyll, protein and RNA consent in addition to RNase activity were studied in the presence of $10{\mu}M$ GA or $10{\mu}M$ ABA in barley coleoptile sections. After 20 hours incubation in the presence of plant hormones, MAK profiles of nucleic acids and reactive distribution of polysome and monosome were investigated. The above results were summarized as follows. 1) The production of ${\alpha}-amylase$ by treatment with GA alone increased at a linear rate in the incubation period and the active secretion of ${\alpha}-amylase$ began from 18 hours incubation in embryoless half seeds. 2) On the contrary to the partial inhibition by addition of $0.1{\mu}M$ ABA, the production of ${\alpha}-amylase$ was completely inhibited by both $5{\mu}M$ and $10{\mu}M$ ABA within 4 hours. Regardless of concentration of GA, the addition of $5{\mu}M$ ABA in midcourse completely inhibited the production of ${\alpha}-amylase$ 3) ABA treatment gave no effect on the secretion of ${\alpha}-amylase$. 4) There were no differences in RNA fractions between GA treatment and concomitant treatment with GA and ABA in the barlye embryoless half seeds. 5) While GA treatment increased the r-RNA fraction, ABA treatment decreased it and increased the s-RNA fraction in the coleoptile sections. 6) GA treatment increased RNA-DNA fraction best ABA treatment decreased it in the coleoptile sections. 7) While GA treatment suppressed RNase activity, ABA treatment increased it in the coleoptile sections. 8) GA treatment gave no great effect on the total RNA but ABA treatment remarkably diminished it in the coleoptile sections. 9) While GA treatment increased the growth and chlorophyll content, ABA treatment decreased them in the coleoptile sections. 10) GA treatment increased the protein synthesis and polysome formation but ABA treatment decreased them in the coleoptile sections. 11) The inhibition effect of ABA on polysome formation seemed to be resulted from the inhibition of r-RNA synthesis by ABA.

• Journal of the Korean Society for Marine Environment & Energy
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• v.13 no.1
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• pp.1-11
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• 2010

In order to understand the biological environmental characteristics with temporal variations of the physico-chemical factors in 2012 Yeosu Expo site of Korea, we investigated at one station, once per week, from April 2006 to December 2007. The surface water temperature ranged from 6.8 to $27.8^{\circ}C$ and the bottom water temperature ranged from 6.3 to 25.9 $25.9^{\circ}C$. The salinity varied from 12.8 to 33.0 psu in the surface water and from 25.2 to 33.6 psu in the bottom water. A strong halocline was observed between the surface and bottom layers in the summer when a rapid decrease of salinity coincided with heavy rainfall. The DIN concentration ranged from 1.36 to $82.7{\mu}M$ in the surface water and from 0.82 to $25.2{\mu}M$ in the bottom water. Phosphate concentration varied from 0.06 to $2.13{\mu}M$ in the surface water and from 0.07 to $1.38{\mu}M$ in the bottom water. Silicate was $1.68-52.0{\mu}M$ in the surface water and $1.37-30.7{\mu}M$ in the bottom water. The nutrient concentrations were generally high during heavy rainfalls and low water temperature periods, and considerably decreased in spring and autumn. The N/P ratio ranged from 4.43 to 325 in the surface water and from 3.8 to 321 in the bottom water. It increased rapidly during the heavy rainfall season and remained at a value of approximately 16 in other periods. The chlorophyll a concentration ranged from 0.46 to $65.0{\mu}g$ $L^{-1}$ in the surface water and from 0.71 to $15.0{\mu}g$ $L^{-1}$ in the bottom water. $Chl-{\alpha}$ concentration remained low in periods of low water temperature, however rapidly increased in periods of high water temperature. From the results of principal component analysis (PCA) and multiple regression analysis (MRA), we conclude that temporal variations of physico-chemical and biological factors were greatly affected by the influx of fresh water, and that nutrients were well controlled by their uptake and assimilation by phytoplankton. Also, during the low water temperature periods, environmental structure in this study site was affected by recycled nutrients through nutrient cycling and mineralization.

• Journal of Plant Biology
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• v.18 no.1
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• pp.1-10
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• 1975

Development of a model system for mode of action studies of $SO_2$ was attempted with a plant tissue. Leaf disks, 1.0cm diameter, cut from the lamina of lettuce leaves, were floated on the testing medium and placed in light or dark condition to investigate the discoloration pattern with various sources of $SO_2$. Discoloration of leaf disks tended to be more serious with higher concentrations of $SO_2$ and on exposure to the light. Leaf disks were more severely discolored at lower pH with constant SO2 concentration. These discoloration patterns were highly reproducible and similar in all sources of $SO_2$. Spectrophotometric evidence suggested that light-mediated discoloration of leaf disks in the presence of $SO_2$ might occur mainly through chlorophyll ${\alpha}$ degradation.

• Korean Journal of Ecology and Environment
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• v.41 no.spc
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• pp.68-76
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• 2008

This study examined the inhibition effects of a freshwater bivalve (Unio douglasiae) and a submerged plant (Potamogeton crispus) on the cyanobacterial bloom (Oscillatoria sp.). The experiment were conducted in aquarium $(50cm{\times}65cm{\times}120cm)$ with lake sediments in the bottom of the aquarium in 10 cm thick. Before the experiments, artificial cyanobacterial bloom was induced with the addition of lake sediment and CB medium. Total 12 transparent acrylic cylinders (${\Phi}19cm$, height 40 cm) were placed in the aquarium, and within which bivalves and plants were placed in various conditions such as the control (C), plant addition (P:5 stems), mussel addition (U:2 individuals), and both mussel and plant addition (PU: the same quantity as used in each treatment). The experiment was conducted in triplicate during 7 days. pH, dissolved oxygen (DO), electric conductivity (EC), salinity, cyanobacterial cell density, chlorophyll-${\alpha}$ concentration, and mussel filtering rate were monitored daily. At the end of the experiment, total phosphorus (TP), total nitrogen (TN), and plant height and weight were measured. Overall, a large degree of cyanobacterial growth inhibition appeared in both P and U treatments, and the effect was highest in the U treatment, followed by P and PU. The combined treatment of both U and P did not show any synergic effects compared to the effect in separated treatment. In all enclosures of the treatments chlorophyll-${alpha}$ (Chl-${alpha}$) concentration decreased until 36 hours after the additions of the plants and mussels. In contrast, Chl-${alpha}$ concentrations increased in PU enclosures after 36 hours. The same trend was shown in the cell density of Oscillatoria. pH and DO gradually decreased until 120 and 144 hours, respectively, in the P and PU enclosures. TP concentration increased in the mussel enclosures (U and PU), while TN concentration largely decreased in the plant enclosures (P and PU). Our results suggest that applied bivalve (Unio) and submerged plant (Potamogeton) seemed to have a potential effect on the growth inhibition of cyanobacteria, but their combined application may have an antagonistic effect to diminish the degree of the inhibition.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.2
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• pp.288-295
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• 1998

Egg production of Acartia steueri was estimated in llkwang Bay, located in the southeastern coast of Korea. The equation, combining fecundity, temperature and chlorophyll a, obtained under the laboratory experiments, was applied to the in situ temperature and chlorophyll a for the estimation of field egg production. Mean egg size was $80.52{\mu}m$. Prosome length of adult females was not correlated with egg size. Egg production of field population ranged from 0.32 to $63.32{\mu}gC\;m^{-3}d^{-1}$ with a mean of $13.33{\mu}gC\;m^{-3}d^{-1}$, which were equivalent to $7.1\~1407.1\;eggs\;m^{-3}d^{-1}$ and $296.1\;eggs\;m^{-3}d^{-1}$, respectively. Fecundity of adult females ranged from 5.4 to $12.5\;eggs\;female^{-1}d{-1}$ with a mean of $8\;eggs\;female^{-1}d^{-1}$. Specific egg production rates ranged from 0.028 to 0.117 $d^{-1}$ with a mean of 0.064 $d^{-1}$. Considering the egg Production ($\%$ female body carbon) as a function of temperature and chlorophlrll a concentration, our results showed rather low fecundity, which might be underestimated. Probable cannibalism of egg by the adults were thought to be pan of the reason for this low estimated fecundity.

• Korean Journal of Environmental Agriculture
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• v.22 no.1
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• pp.1-6
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• 2003

The yearly average water qualities of the Whang river, which flows into the Hapcheon lake, were COD $3.1{\sim}4.2\;mg/L$, T-N $2.460{\sim}3.550\;mg/L$ and T-P $0.111{\sim}0.201\;mg/L$ during $1996{\sim}2001$. The yearly average COD concentration of Hapcheon lake was increased from 1.9 mg/L (in 1996) to 2.7 mg/L (in 2000). However, T-N and T-P concentration of Hapcheon lake did not show increasing trend over the 6 year period During $1996{\sim}2001$, the yearly average concentrations of T-N, T-P were $1.383{\sim}1.792\;mg/L$, $0.018{\sim}0.023\;mg/L$ respectively. The correlation coefficients between chlorophyll ${\alpha}$ and T-N, T-P, rainfall intensity, water temperature were 0.382, 0.372, 0.589, and 0.526, respectively. Therefore, the rainfall and water temperature appeared to play an important role far the variations of chlolophyll ${\alpha}$ concentration in the Hapcheon lake. Trophic state of the Hapcheon lake were evaluated to be in the range of mesotrophic to eutrophic.

• 한국해양학회지
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• v.3 no.1
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• pp.16-25
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• 1968

This study was undertaken to assess the annual cycle of primary production and plant pigments in a downstream of the Han River. Measurements were carried out at three week intervals during April 1966 and March 1967, and ancillary data include water temperature, transparency, pH, dissolved oxygen and phytoplankton cell number. The seasonal cycle in water temperature profile shows the hihgest in the end of August with 27$^{\circ}C$, lowest in the middle of February with 0.2$^{\circ}C$. The transparency with Secchi disk reading varied from a maximum 4.0m in fall and a minimum 0.5m or less in early spring and flood season of summer. The pH of the river water varied from 6.5 to 7.3, averaged 6.91 in the surface water and 6.98 in the bottom water, showed little seasonalvariability. The dissolved oxygen in the surface water ranged from 5.93-9.64ml/L, while in the bottom water it ranged from 5.54-9.72 ml/L, and the oxygen saturation never fall below 94%. None thermal, the distribution of pH and content of oxygen, stratifications occurred. An apparent seasonal cycle of primary productivity was observed with remarkably high levels in the spring and fall, the lowest level in the winter. The range of net carbon assimilations showed 3.1-112.6 mgC/㎥/day or 15-427 mgC/㎡/day in spring, 37.0-271.2 mgC/㎥/day or 115-329 mgC/㎡/day in summer, 27.2-168.0 mgC/㎥ /day or 139-415 mgC/㎡/day in fall and 0.5-10.9 mgC/㎥/day or 5-19 mg/㎡/day in winter, respectively. Amount of chlorophyll ${\alpha}$ ranged from a minimum concentration of 0.2-0.3 mg/㎥ in the middle of February and a maximum 4.1-6.7 mg/㎥ in the middle of June. A general increase trend in chlorophyll ${\alpha}$ concentration was noted with increase of the river water temperature.