• Journal of Environmental Science International
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• v.25 no.3
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• pp.425-438
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• 2016

This study carried out zeta potential measurements of the Microcystis sp. under various solutions condition and investigated the characteristics of Microcystis sp. through the size control of microbubbles to eliminate algae that causes problems in aquatic ecosystems and human activities. DAF process was adopted and several coagulants were used to remove the Microcystis sp. CCD Camera was used to measure and analyze the size of microbubble, and fluorescent microscope was used to observe the particle, algae species and community. Zeta potential behavior of the algae was analyzed by using ELS-Z. Lab-scale and pilot-scale experiments were conducted to test flotation process. Polyaluminium chloride(PAC) coagulant was used, and the removal efficiency of the algae was assessed through Chlorophyll-a analysis. In the Lab-scale experiment, 2.2 ppm, 11 ppm, 22 ppm, and 44 ppm of polyaluminium chloride was injected to coagulate the algae. The coagulated algae was floated by the microbubble. The microbubbles in the experiments were generated at a air pressure of 450 ~ 550 kPa. The microbubble size was controlled in $36{\mu}m$, $100{\mu}m$, and $200{\mu}m$, respectively by using different diffuser. The results of lab-scale experiments on flotation plant indicated that the average removal rate was about 90% or above for 11 ppm, 22 ppm, and 44 ppm of polyaluminium chloride. On the other hand, in the pilot-scale experiment, the removal efficiency was in the range of 85% to 95% in all dose ranges of polyalumium chloride and aluminium sulfate coagulants.

• Journal of Plant Biology
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• v.33 no.4
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• pp.277-283
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• 1990

Inactivation and reactivation of photosynthetic oxygen evolving complex were studied with isolated spinach (Spinacia oleraceda. L.) photosystem II particles by the activity of oxygen evolution and chlorophyll fluorescence. When the particles were treated with Tris and urea, the oxygen evolution was inactivated and three polypeptides having molecular weights of 33 kDa, 24 kDa and 18 kDa were simultaneously released. But in NaCl-treated particles, two polypeptides of 24 kDa and 18 kDa were removed from PS II particles. The oxygen evolution activities of Tris and urea-treated particles were not restored by adding cation ions (Mg2+, Mn2+ and Ca2+), but the NaCl-treated particles were restored by exogenously added Ca2+. The removal of these extrinsic polypeptides, especially 33 kDa, markedly showed the decrease of the variable fluorescence (Fv). These results are likely to be due to dissipate thermal energy by antenna of photosystem II complexes.

• Journal of Microbiology and Biotechnology
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• v.26 no.3
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• pp.503-510
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• 2016

The accumulation (internal and superficial distribution) of magnesium ions (Mg²⁺) by the green freshwater microalga Chlorella vulgaris (C. vulgaris) was investigated under autotrophic culture in a stirred photobioreactor. The concentrations of the three forms of Mg²⁺ (dissolved, extracellular, and intracellular) were determined with atomic absorption spectroscopy during the course of C. vulgaris growth. The proportions of adsorbed (extracellular) and absorbed (intracellular) Mg²⁺ were quantified. The concentration of the most important pigment in algal cells, chlorophyll a, increased over time in proportion to the increase in the biomass concentration, indicating a constant chlorophyll/biomass ratio during the linear growth phase. The mean-average rate of Mg²⁺ uptake by C. vulgaris grown in a culture medium starting with 16 mg/l of Mg²⁺ concentration was measured. A clear relationship between the biomass concentration and the proportion of the Mg²⁺ removal from the medium was observed. Of the total Mg²⁺ present in the culture medium, 18% was adsorbed on the cell wall and 51% was absorbed by the biomass by the end of the experiment (765 h). Overall, 69% of the initial Mg²⁺ were found to be removed from the medium. This study supported the kinetic model based on a reversible first-order reaction for Mg²⁺ bioaccumulation in C. vulgaris, which was consistent with the experimental data.

• Journal of Wetlands Research
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• v.7 no.3
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• pp.67-73
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• 2005

At research, the river's water which the sewage treatment facility will ask growth suppression the algae in the artificial rivers which it is using or it researched the method for the removal of the algae which grew. At results, when 60ppm of Chlorine was put in, the possibility of getting the result where 90% chlorophyll-a was removed and LC50 was 0.8ppm. The algae did not create even after 20days by which uses prevent agent of creation of the algae. Controlling the chlorine almost there was not an extinction effect of the algae which was already in large quantities multiplied, with in the effect against an water ecosystem widely known it is not. The prevent agent could inhibit creation of the algae, but it was judged with the fact that the methods are directly applied to the field must be researched continuously.

• Korean Journal of Environmental Agriculture
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• v.17 no.4
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• pp.352-357
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• 1998

This experiment was conducted to find out the effects of major nutrient levels(N, P, K) on ozone susceptibility of tomato plants(Lycopersicon esculentum Mill, cv. Pink Glory). Plants were grown in water culture system. A half-strength of Hoagland's nutrient solution was considered as a standard formulation($N_{100}$ $P_{100}$ $K_{100}$). The levels of major nutrients were adjusted through addition or removal of several fertilizer salts from the standard solution. Top growth was significantly decreased at the low nitrogen level or phosphorus removal condition. P- and K-contents of leaves were greatly decreased by removal of salts containing P and K from the nutrient solution. The rate of ozone injury was significantly increased when potassium was removed. However, the influence of nitrogen and phosphorus levels or high potassium level on injury occurrence did not show statistical significance compared to the standard solution. Ozone exposure resulted in reduction of chlorophyll, and increase of ethylene production, electrolyte leakage and malondialdehyde(MDA) contents. These changes were much more enhanced in plants grown at the potassium removal solution. Whereas the activity of superoxide dismutase(SOD) was low at the potassium removal treatment and this tendency remained after ozone exposure. These results indicated that potassium nutrient level in tomato plants is closely associated with the susceptibility to ozone injury.

• Korean Journal of Ecology and Environment
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• v.42 no.2
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• pp.161-171
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• 2009

A 'continuous removal of organic matters (CROM) system' using a native freshwater bivalve in Korea Anodonta woordiana, was developed to determine its potential of controlling various sestons in eutrophic lake system, and to evaluate its effect on water quality improvement under consideration of sediment addition as habitat. We designed CROM experiments with four treatments: no mussels and no sediment (W, negative control), no mussels and sediment (WS, positive control), mussels and no sediment (WM), and mussels and sediment (WMS). The experiments were performed at the condition of 18${\sim}$25 L $h^{-1}$ of inflow, mussel density of 486.1 indiv. $m^{-2}$, and temperatures between 15 and $22^{\circ}C$ for 13 consecutive days. Physicochemical and biological parameters were measured at daily (10:00 am) intervals after the mussel addition. Results indicated that mussel stockings without addition of sediment effectively removed sestons (suspended solids and chlorophyll-a) at nearly same level over 80 percentage of the control during the study, while there were no differences in removal activities of sestons between with and without sediment (P>0.5). Therefore, it clearly suggests that CROM system using A. woordiana has a strong potential to control the seston in surface water of eutrophic lake.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.28 no.5
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• pp.589-598
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• 1995

Filtering rates of Crassostrea gigas were experimentally investigated with reference to effects of water temperature and size. Absorptiometric determinations of filtering rates with oysters being fed diatom Chaetoceros calcirtans were carried out in a closed system. Optical density of 675nm in path length 100mm cell used as the indication of food particles absorption was appeared directly In proportion with the concentration of diatom pigment $chlorophyll-\alpha$. In the closed system where $C_0$ is $OD_{675}$ at initial time 0, $C_t$, at time t, and Z is the decreasing coefficient of OD as meaning of instantaneous removal speed, then $C_t=C_0{\cdot} e^{-2t}$, $Z=In(C_t/C_0)/t$. On the assumption that the filtering rate is constant, then removal rate per unit time (d) is $d=-e^{-z}$. If t is used to time unit of hour (hr), the filtering rate (FR) in I/hr is given by $FR=V{\cdot}d=V(1-e^{-z})$, where V is the water volume (I) of the experimental vessel. Filtering rate increased as exponential function with increasing temperature while not over critical limit. The critical temperature for filtering rate was assumed to be between $28^{\circ}C$ and $29^{\circ}C$. And the weight exponent for filtering rate is 0.223. The model formula derived from the results as FR, $Ihr^{-1}$ = $Exp(0.208{\cdot}T-4.324){\cdot} (DW)^{0.223}$ (T<29 $^{\circ}C)$ where T is water temperature $(^{\circ}C

• Journal of Environmental Science International
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• v.10 no.1
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• pp.21-25
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• 2001

The Keum river has been utilized for drinking water supply of several city including Kunsan city and is deepening pollution state due to numerous municipal and industrial discharges. The concentration BOD in river is affected by the organic loading from a tributary and the algae biomass that largely happen to under eutrophication state. In the eutrophic water mass such as the Keum river, the autochthonous BOD was very important part for making a decision of water quality management, because it was accounted for majority of the total BOD. The predict of water quality has important meaning for management of water quality pollution of the Keum river. The purpose of this study will manage and predict water quality of the Keum river using QUAL-2E model considering the autochthonous BOD. The estimation of autochthonous BOD represented that the relationship between BOD and chlorophyll a. The regression equation was shown to be autochthonous BOD=$\beta$(sub)5$\times$chlorophyll a. The results of this study may be summarized as followed; The QUAL-2E model was calibrated with the data surveyed in the field of the study area in June, 1998. The calculated value by QUAL-2E model are in good agree to measured value within relative error of 7.80~20.33%. Especially, in the case of the considering autochthonous BOD, the calculated value of BOD were fairly good coincided with the observed values within relative error of 15%. But the case of not considering autochthonous BOD, relative error of BOD was shown to be 43.2%. In order to attain II grade of water quality standard in Puyo station which has a intake facility of water supply, we reduced to the pollutants loading of tributaries. In the case of removed 100% BOD of tributaries, the BOD of Puyo station was 4.07mg/$\ell$, belong to III grade of water quality standard. But in the case of removed 88% nutrient of tributaries, it was satisfied to II grade of water quality standard as below 3mg/$\ell$ of BOD. For estimation of autochthonous BOD in Keum river, we are performed simulating in accordance with reduction of nutrient load(50~100%) under conditions removal 90% organic load. Occupancy of autochthonous BOD according to nutrient loading reductions were varied from 25.97~79.51%. Occupancy of autochthonous BOD was shown to be a tendency to increasing in accordance with reduction of nutrient loading. Showing the above results, the nutrient that one of the growing factor of algae was important role in decision of BOD in the Keum river. For the water quality management of the Keum river, therefore, it is necessary to considering autochthonous BOD and to construction of advanced sewage treatment plant for nutrient removal.

• Journal of Environmental Impact Assessment
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• v.27 no.1
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• pp.83-91
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• 2018

Media (bio-stone), aquatic macrophytes (Oenanthe javanica) and herbivorous cladoceran (Daphnia similoides) have been used in artificial floating island (AFI) systems for water pollution control. Efficiency in chl-a concentration controlling of AFI was tested using different combinations of each device: G-BD-mixture bio-stone and Daphnia similoides, G-OB-mixture Oenanthe javanica and bio-stone, G-BOD-mixture bio-stone, Oenanthe javanica and Daphnia similoides, and the out-put water quality improvement was compared with G-C-control (no device was applied). We analyzed removal efficiency of chl-a concentration and nutrient concentrations in the artificially eutrophic water in the laboratory experimental facility. The results showed average removal rates of Chlorophyll a, TN and TP for different four groups: 69.24%, 16.61%, -0.61%; 68.39%, 14.11%, 10.52%; 78.30%, 6.69%, 25.09%; 35.42%, -3.47%, -25.18%, respectively. The results have suggested that the mixture of media, plants and zooplankton is the most efficient combination for Chlorophyll a control, while the mixture of macrophytes and bio-stone have better efficiency nutrient control.

• Journal of Korean Society of Environmental Engineers
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• v.32 no.3
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• pp.248-255
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• 2010

This study was carried out to assess the feasibility of the electron beam irradiation as a mean of red tide control in coastal water. Prorocentrum minimum, Prorocentrum micans, Cochlodinium polykrikoides, Heterosigma akashiwo, Alexnadrium catenella were selected and cultured for experiments, and red tide occurring in Tongyeong(2007. 8. 15) was also tested under the same conditions. The irradiation dose were 1 kGy, 2 kGy, 4 kGy and 8 kGy. The result showed 50~65% extinction in red tide cells was observed right after irradiation dose of 1 kGy and 86~97% within 1 day after irradiation, compared with control. Chlorophyll-a concentration of red tide was reduced by 50~64% immediately and it was drastically reduced up to 86~97% 1 day after irradiation. When the culture was irradiated at 1 kGy, 28~47% of s-protein was released immediately, and 77~138% was released 1day after irradiation. 77~212% of s-carbohydrate was excreted after 1 day while 16~45% of s-carbohydrate was excreted immediately. A transmission electron microscope(TEM) observation for the irradiated red tide revealed that the cell was destroyed and intracellular biopolymeric substance was leached out from the damaged cell as a result of electron beam irradiation. These results imply that electron beam irradiation is enable to control red tide by flocculation with extracellular biopolymer. The paralytic shellfish poisoning(PSP) toxin contents produced by Alexandrium catenella was decreased 48% by 1 kGy of electron beam irradiation compared with the unirradiated cells. As a result, electron beam irradiation was effective for detoxication as well as destruction of red tide.