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http://dx.doi.org/10.4014/jmb.1507.07039

Magnesium Uptake by the Green Microalga Chlorella vulgaris in Batch Cultures  

Ayed, Hela Ben Amor-Ben (CentraleSupelec, Grande Voie des Vignes)
Taidi, Behnam (CentraleSupelec, Grande Voie des Vignes)
Ayadi, Habib (Faculty of Sciences of Sfax, Laboratory of Biodiversity and Aquatic Ecosystems, Ecology and Department of Planktonology, University of Sfax)
Pareau, Dominique (CentraleSupelec, Grande Voie des Vignes)
Stambouli, Moncef (CentraleSupelec, Grande Voie des Vignes)
Publication Information
Journal of Microbiology and Biotechnology / v.26, no.3, 2016 , pp. 503-510 More about this Journal
Abstract
The accumulation (internal and superficial distribution) of magnesium ions (Mg2+) by the green freshwater microalga Chlorella vulgaris (C. vulgaris) was investigated under autotrophic culture in a stirred photobioreactor. The concentrations of the three forms of Mg2+ (dissolved, extracellular, and intracellular) were determined with atomic absorption spectroscopy during the course of C. vulgaris growth. The proportions of adsorbed (extracellular) and absorbed (intracellular) Mg2+ were quantified. The concentration of the most important pigment in algal cells, chlorophyll a, increased over time in proportion to the increase in the biomass concentration, indicating a constant chlorophyll/biomass ratio during the linear growth phase. The mean-average rate of Mg2+ uptake by C. vulgaris grown in a culture medium starting with 16 mg/l of Mg2+ concentration was measured. A clear relationship between the biomass concentration and the proportion of the Mg2+ removal from the medium was observed. Of the total Mg2+ present in the culture medium, 18% was adsorbed on the cell wall and 51% was absorbed by the biomass by the end of the experiment (765 h). Overall, 69% of the initial Mg2+ were found to be removed from the medium. This study supported the kinetic model based on a reversible first-order reaction for Mg2+ bioaccumulation in C. vulgaris, which was consistent with the experimental data.
Keywords
Chlorella vulgaris; metal ions uptake; adsorbed Mg2+ (extracellular); absorbed Mg2+ (intracellular); photobioreactor;
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