• Journal of Korean Society of Forest Science
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• v.99 no.5
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• pp.711-719
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• 2010

We investigated physiological responses and phytoextraction ability of Pinus thunbergii in cadmium contaminated soil as part of our efforts in identifying plant materials for the restoration and revegetation of forest soil contaminated by heavy metals. Thirty seedlings (ten per treatment) were assigned to three treatments (control, 0.3 and 0.6 mM $CdSO_4$ solution) at first year experiment. At second year, ten seedlings per treatment treated with Cd during the first year experiment were divided by two groups (no Cd-treated and consecutive Cd-treated group). At first experiment, photosynthetic pigment content, and superoxide dismutase (SOD) and glutathione reductase (GR) activities have significantly reduced by Cd application, and the reduction rate was increased much higher as the rate of Cd application increased. On the other hand, thiol and malondialdehyde (MDA) content were significantly increased at the application of 0.6 mM of Cd. At the second year experiment, a general increase in chlorophyll and carotenoid content was observed with Cd treatment while SOD and GR activities showed a relative reduction compared to the control. Similar to the first year measurement, thiol and MDA contents also increased considerably due to Cd treatment. At harvest, dry matter was significantly reduced by Cd treatment especially at the rate of 0.6 mM Cd, but dry yield of P. thunbergii treated with 0.3 mM Cd was less affected and it was comparable with the control seedling. Cadmium concentration in seedling tissues increased with increasing Cd application rate while Cd uptake was higher in seedlings supplied with 0.3 mM Cd, which could be ascribed to their high dry matter. Overall, our study has demonstrated the unique physiological response of P. thunbergii to Cd-prolonged exposure by showing that the changes in photosynthetic pigment content and antioxidative enzyme activities were dependent on the concentration and duration of treatment. In addition, our results have demonstrated the potential of P. thunbergii to withstand up to 0.3 mM Cd (equivalent to cumulative Cd concentration of 134.4 to 268 mg $kg^{-1}$) without showing growth reduction, hence it might be used for phytoremediation of Cd contaminated areas.

• Applied Biological Chemistry
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• v.29 no.2
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• pp.219-226
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• 1986

The differences of pigment compositions in leaf chloroplast between sun and shade plant were analyzed to investigate the photo-induced destructive effects on shade species under the strong light. Ginseng was selected as a C-3 shade plant, soybean as C-3 sun species and corn as C-4 malate forming sun species. To study the effects of light, ginseng was divided into two subgroups; the 1 and 2 lines received sunlight a little more than those of 3 and 4 lines. Total amounts of chlorophylls, carotenes and xanthophylls were not considerably different among 3 and 4-lined ginseng, soybean and corn. However, the amounts of the three components of 1 and 2-lined ginseng and the chlorophylls content in corn leaves were smaller than those of others. The molar ratio of lutein to total carotenoids was significantly high in ginseng, that of violaxanthin was considerably high in corn and that of neoxanthin the highest in 1 and 2-lined ginseng among tested samples. Chlorophylls to carotenes ratio was 16.0 and highest in ginseng, 13.2 in soybean and 12.0 in corn. In 1 and 2-lined ginseng, the molar ratios of lutein and carotenes were lower while those of neoxanthin and violaxanthin were higher than those of the samples of more light. It was noticeable that an antheraxanthin-like epoxy carotenoid detected in soybean and corn leaves extracts was not observed in ginseng leaf extract.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.28 no.5
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• pp.589-598
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• 1995

Filtering rates of Crassostrea gigas were experimentally investigated with reference to effects of water temperature and size. Absorptiometric determinations of filtering rates with oysters being fed diatom Chaetoceros calcirtans were carried out in a closed system. Optical density of 675nm in path length 100mm cell used as the indication of food particles absorption was appeared directly In proportion with the concentration of diatom pigment $chlorophyll-\alpha$. In the closed system where $C_0$ is $OD_{675}$ at initial time 0, $C_t$, at time t, and Z is the decreasing coefficient of OD as meaning of instantaneous removal speed, then $C_t=C_0{\cdot} e^{-2t}$, $Z=In(C_t/C_0)/t$. On the assumption that the filtering rate is constant, then removal rate per unit time (d) is $d=-e^{-z}$. If t is used to time unit of hour (hr), the filtering rate (FR) in I/hr is given by $FR=V{\cdot}d=V(1-e^{-z})$, where V is the water volume (I) of the experimental vessel. Filtering rate increased as exponential function with increasing temperature while not over critical limit. The critical temperature for filtering rate was assumed to be between $28^{\circ}C$ and $29^{\circ}C$. And the weight exponent for filtering rate is 0.223. The model formula derived from the results as FR, $Ihr^{-1}$ = $Exp(0.208{\cdot}T-4.324){\cdot} (DW)^{0.223}$ (T<29 $^{\circ}C)$ where T is water temperature $(^{\circ}C

• Journal of Korean Society of Forest Science
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• v.101 no.2
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• pp.236-243
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• 2012

The leaf growth and physiological characteristics of Forsythia saxatilis were investigated at six natural habitats under different light intensities in Bukhansan in order to figure out an appropriate growth environment for conservation and restoration of F. saxatilis that is Korean endemic plant designated as rare and endangered species. Relative light intensities (RLI) at six habitats showed from 10% to 78% of the full sun. Leaf length, leaf width, leaf area and dry leaf weight of population under highest relative light intensity (78%) were the highest. The ratio of dry leaf weight to leaf area increased with the increase of RLI. The content of photosynthetic pigments such as chlorophyll a, b and carotenoid were the highest at 78% of RLI, whereas the ratio of total chlorophyll to carotenoid content reduced according to the increase of RLI. Photosynthetic parameters, such as photosynthetic rate, also increased with the increase of RLI. The total nonstructural carbohydrate (TNC) was 1.5 times higher at 78% of RLI than that at 10% of RLI and the total soluble sugar (TSS) was the highest at 78% of RLI. In conclusion, leaf characteristics and physiological characteristics have high positive correlation with light intensity. Therefore, light condition should be primarily considered to improve growth and physiology characteristics of F. saxatilis under low light intensity.

• Journal of Environmental Science International
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• v.24 no.11
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• pp.1501-1511
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• 2015

As essential trace elements, copper and zinc play important roles in many physiological events in plants. In excess, however, these elements can limit plant growth. This study selected a heavy metal-tolerant plant by analyzing seed germination and biomass of alfalfa (Medicago sativa), canola (Brassica campestris subsp. napus var. nippo-oleifera), Chinese corn (Setaria italica), and a sorghum-sudangrass hybrid (Sorghum bicolor ${\times}$ S. sudanense), and determined heavy metal uptake capacity by analyzing biomass, chlorophyll a fluorescence, and heavy metal contents under high external copper or zinc levels. The seed germination rate and biomass of the sorghum-sudangrass hybrid were higher under copper or zinc stress compared to the other three plants. The plant biomass and photosynthetic pigment contents of the sorghum-sudangrass hybrid seedlings were less vulnerable under low levels of heavy metals (${\leq}50ppm$ copper or ${\leq}400ppm$ zinc). The maximum quantum yield of PSII ($F_v/F_m$) and the maximum primary yield of PSII ($F_v/F_o$) decreased with increasing copper or zinc levels. Under high copper levels, the decline in $F_v/F_m$ was caused only by the decline in $F_m$, and was accompanied by an increase in non-photochemical quenching (NPQ). The $F_v/F_m$ declined under high levels of zinc due to both a decrease in the maximum fluorescence ($F_m$) and an increase in the initial fluorescence ($F_o$), and this was accompanied by a marked decrease in photochemical quenching (qP), but not by an increase in NPQ. Accumulations of copper and zinc were found in both aboveand below-ground parts of plants, but were greater in the below-ground parts. The uptake capacity of the sorghum-sudangrass hybrid for copper and zinc reached 4459.1 mg/kg under 400 ppm copper and 9028.5 mg/kg under 1600 ppm zinc. Our results indicate that the sorghum-sudangrass hybrid contributes to the in situ phytoremediation of copper or zinc polluted soils due to its high biomass yield.

• Journal of the Korean Society for Marine Environment & Energy
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• v.19 no.2
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• pp.144-150
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• 2016

In southwestern East Sea, we investigated the spatio-temporal distribution characteristics of particulate organic carbon (POC) and nitrogen (PON) in September 2011 (summer), January (winter) and May 2012 (spring). Although cold waters known as the origin upwelling in the surface layer of September were not observed, this periods showed high primary productivity because of high concentrations of chlorophyll, low percentage of non-autotrophic particulate fraction among POC calculated by POC/Chl-a ratio (27%) and low POC/PON ratio (6.2), which means active amino acid and protein synthesis, However, May, 2012 showed low primary productivity because of high percentage of non-autotrophic particulate fractions among POC (66%) and high POC/PON ratio (8.1), Although spring bloom and high primary productivity has been reported in the East Sea, high percentage of non-autotrophic particulate fractions in POC, observed in the East sea during the post 2012 spring, is suggested to be due to the increase of phaeo-pigment during post spring bloom. Thus, composition of particulate organic matter may have sensitively changed by marine environmental factors in spite of same season.

• The Korean Journal of Food And Nutrition
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• v.27 no.3
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• pp.339-347
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• 2014

This study investigated the classification of olive oils that are mainly distributed in Korea via imports. The fatty acid contents, degree of color, pigments, anti-oxidants, and sterol contents are analyzed on the different types of olive oil as follows: 10 kinds of extra virgin olive oil, 5 kinds of pure olive oil, and 5 kinds of refined olive-pomace oil. As a result of fatty acid analysis, the majority of oleic acid ($C_{18:1}$) and palmitic acid ($C_{16:0}$), and minority of linoleic acid ($C_{18:2}$) and stearic acid ($C_{18:0}$) were detected without any significant differences between the grades of olive oils. The UV spectrum is related to the ${\Delta}K$, and it is a part of the analysis factor for the purity and degree of degradation of the oil. Extra virgin olive oil had ${\Delta}K$ of almost 0, pure olive oil had 0.07~0.12, and refined olive-pomace oil had 0.1~0.13. These differed from extra virgin oil, and the pure or pomace oil ${\Delta}K$ had a confirmed distinct difference. The color degrees of chlorophyll with a low $L^*$ value and $(-)a^*$ (green) and carotenoid with $(+)b^*$ (yellow) were confirmed to have correlation between extra virgin and other olive oils. To compare chlorophyll and carotenoid as natural pigment in olive oils, 417 nm and the ratio of the absorbance at 480 nm (417/480) was calculated at 1.62 of extra virgin, 1.85 of pure olive oil, and 3.32 of refined olive-pomace oil. Therefore, it will be possible to distinguish when the extra virgin or pure olive oil are mixed with olive-pomace oil. The total amount of tocopherol, an anti-oxidant, were 19.06 in extra virgin, 10.91 in pure olive oil, and 27.88 in refined olive-pomace oil. The high content of tocopherol in pomace oil caused recovery of solvent extraction from olive pulp. Thus, extra virgin oil and pure olive oil were distinguished by olive-pomace oil. Polyphenol compounds in extra virgin olive oil measured high only in ferulic acid with 0.543 mg/kg, caffeic acid with 0.393 mg/kg, and other vanillic acid, vanillin, and p-coumaric acid had similar amount of 0.3 mg/kg. All grade of olive oils had the highest ${\beta}$-sitosterol content. Af (Authenticity factor) value were estimated with campesterol and stigmasterol content ratio (%). Af value was 19.2 in extra virgin olive oil, 17.1 in pure olive oil, 16.9 in refined olive-pomace oil, which were distinctive from sunflower oil with 3.7, corn oil with 2.4, and soybean oil with 2.0. It can provide important indicator of olive oil adulteration with other cheap vegetable oils. The results of this study can be used as a database for the classification of olive oil grade and distinguishing between the different types of oils.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.10 no.3
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• pp.163-170
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• 1977

The present study was carried out to knew the sterol components of U. pinnatifida and their incorporation abilities of $^14C-1-acetate$ injected into it. The results obtained are as follows: 1. The total lipids are classified as hydrocarbon $1.6\%$, pigment and sterol ester $2.5\%$, triglyceri do $3.3\%$, free fatty acid $2.2\%$, free sterol $3.8\%$, chlorophyll $18.8\%$, and polar lipids $ 67.3\%$ 2. The sterol mixture from U. pinnatifida are omposed of cholesterol $3.5\%$, 24-methylene-cholesterol $11.2\%$, fucosterol $85.3\%$. 3. The radioactivities of the lipids classes from U. pinnatifida injected with $^14C-1-acetate$ are distributed 4,648 dpm/ug in total lipid, 2,754 dpm/mg in polar lipids, 373 dpm/mg in chlorophyll, 22,481 dpm/mg in free sterol, 6,520 dpm/mg in free fatty acid, 789 dpm/mg in sterol ester and 358 dpm/mg in hydrocarbon respectively. 4. The specific radioactivities of the sterols are 115 dpm/mg in cholesterol, 147,821 dpm/mg in 24-methylenecholesterol, 20, 887 dpm/mg in fucosterol.

• Korean Journal of Agricultural and Forest Meteorology
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• v.16 no.1
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• pp.22-28
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• 2014

This study was conducted to investigate the photosynthetic characters of Populus alba${\times}$glandulosa cuttings in response to elevated $CO_2$ concentration and air temperature for selecting tree species adaptive to climate change. The cuttings were grown in environment controlled growth chambers with two combinations of $CO_2$ concentration and air temperature conditions: (i) $22^{\circ}C$ + $CO_2$ 380 ${\mu}mol$ $mol^{-1}$ (control) and (ii) $27^{\circ}C$ + $CO_2$ 770 ${\mu}mol$ $mol^{-1}$ (elevated) for almost three months. The cuttings under the elevated treatment showed reduced tree height and photosynthetic pigment contents such as chlorophyll and carotenoid. In particular, the elevated treatment resulted in a marked reduction in the chlorophyll a closely associated with $CO_2$ fixative reaction system. Different levels of reduction in photosynthetic characters were found under the elevated treatment. A decrease was noted in photochemical reaction system parameters: net apparent quantum yield (7%) and photosynthetic electron transport rate (14%). Moreover, a significant reduction was obvious in $CO_2$ fixative reaction system parameters: carboxylation efficiency (52%) and ribulose-1,5-bisphosphate(RuBP) regeneration rate (24%). These results suggest that the low level of photosynthetic capacity may be attributed to the decreased $CO_2$ fixative reaction system rather than photochemical reaction system.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.24 no.2
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• pp.282-297
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• 2019

Detection of mesoscale oceanic eddies using satellite data can utilize various ocean parameters such as sea surface temperature (SST), chlorophyll-a pigment concentration in phytoplankton, and sea level altimetry measurements. Observation methods vary for each satellite dataset, as it is obtained using different temporal and spatial resolution, and optimized data processing. Different detection results can be derived for the same oceanic eddies; therefore, fundamental research on eddy detection using satellite data is required. In this study, we used ocean color satellite data, sea level altimetry data, and infrared SST data to detect mesoscale eddies in the East Sea and compared results from different detection methods. The sea surface current field derived from the consecutive ocean color chlorophyll-a concentration images using the maximum cross correlation coefficient and the geostrophic current field obtained from the sea level altimetry data were used to detect the mesoscale eddies in the East Sea. In order to compare the eddy detection from satellite data, the results were divided into three cases as follows: 1) the eddy was detected in both the ocean color and altimeter images simultaneously; 2) the eddy was detected from ocean color and SST images, but no eddy was detected in the altimeter data; 3) the eddy was not detected in ocean color image, while the altimeter data detected the eddy. Through these three cases, we described the difficulties with satellite altimetry data and the limitations of ocean color and infrared SST data for eddy detection. It was also emphasized that study on eddy detection and related research required an in-depth understanding of the mesoscale oceanic phenomenon and the principles of satellite observation.