• Title/Summary/Keyword: chlorophyll a and b

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Effect of Metal Ions on Stabilization of Codium fragile's Pigments (금속이온이 청각 색소의 안정화에 미치는 영향)

  • Lee, In-Seon;Lee, Hong-yeol;Kim, Hag-Lyeol;Ko, Kang-Hee;Chang, Hae-Choon;Kim, In-Cheol
    • Food Science and Preservation
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    • v.15 no.3
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    • pp.352-360
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    • 2008
  • The extraction yield and storage stability of Codium fragile pigments extracted in acetone, ethanol or methanol were studied. Methanol was the most effective solvent for pigment extraction, providing an extraction yield of $25.0{\pm}2.10\;mg/g$ (my base). As shown by TLC and HPLC analysis, chlorophyll a(0.40 mg/g) and chlorophyll b(1.94 mg/g) were the major pigment components in dried Codium fragile. The total chlorophyll content of Codium fragile stored a 40C in light or dark conditions for 30 weeks remained at 23.2% and 58.4% respectively. The effect of metal ions ($Cu^{++}$, $Zn^{++}$, $Fe^{++}$ and $Mg^{++}$) on pigment stability was analyzed Among the four metal ions $Cu^{++}$ was the most effective stabilizer of Codium fragile pigments during storage, and $Zn^{++}$ ion was the second most effective. In the presence of 1 mM $Cu^{++}$, the total chlorophyll retained in Codium fragile stored at 40C in light or dark conditions was increased to 47.0% and 88.8% after 30 weeks storage, respectively. The optimum concentrations of $Zn^{++}$ and $Cu^{++}$ for pigment stabilization under dark conditions were 0.5 mM and 0.1 mM, respectively.

Changes of Vitamin C and Chlorophyll Contents in Oi-Kimchi with Storage time (저장기간에 따른 오이김치의 비타민 C 및 클로로필 함량변화)

  • Park, Mi-Lan;Lee, Yeon-Jung;Kozukue, Nobuyuki;Han, Jae-Sook;Choi, Suk-Hyun;Huh, Sung-Mee;Han, Gyeong-Phil;Choi, Soo-Keun
    • Journal of the Korean Society of Food Culture
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    • v.19 no.5
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    • pp.566-572
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    • 2004
  • This study was carried out to examine the changes of pH, chlorophyll, chlorophyll patterns and ascorbic acid contents, and sensory evaluation in Oi-Kimhi by the different periods of storage. The contents of chlorophyll a and b were 52.2% and 14.44% at storage 0 days, 20.67% and 16.99% at storage 11 days,0% at storage 14 days, and decreased with storage times increased. But, phephorbide a and b were increased with storage times increased until storage 11 days, and were 28.44% and 1.25% at storage 0 days,42.63% and 15.79% at storage 11 days,0% at storage 12, 14 days. The contents of ascorbic acid were increased until storage 4 days, but afterwards were decreased with storage times increased, and were 2.57mg% at storage 0 days, 5.49mg% and 2.95mg% at storage 11 days, 0% at storage 14 days. Sensory evaluations of appearance, flavor, taste, texture, overall acceptability showed the highest at storage 8 days, and disliked after storage 10 days.

Evaluation of Internal Quality of Kale Leaf by Non-Destructive Color Measurement (비파괴적 엽색분석을 통한 케일 잎의 내적품질 평가)

  • Kang Ho-Min;Kim IL Sop;Won Jae Hee
    • Journal of Bio-Environment Control
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    • v.14 no.3
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    • pp.144-148
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    • 2005
  • Kale leaf had similar contents of vitamin C, $NO_3$, p, Ca, Mg, and Fe with reported values. Among these internal quality factors, $NO_3$ content which has been a concern recently, ranged from 139 to 429 mg in 100 g fresh kale leaf, Kale showed high vitamin C content ranging from 106 to 203 mg in 100 g fresh leaf. The relative concentration of chlorophyll and b value color had a high correlation coefficient (r) with vitamin C, $NO_3$, Mg and Fe content. The relationship between $NO_3$ content and the relative concentration of chlorophyll was given by the following linear equation: $NO_3$ content : 21.55 + (5.907 ${\times}$ the relative concentration of chlorophyll) with an r of $0.910^{{\ast}{\ast}}$. Correlation between $NO_3$ content and b value was also very dependable (r = $-0.901^{{\ast}{\ast}}$). Vitamin C content and the relative concentration of chlorophyll showed a high correlation, r = $-0.858^{{\ast}{\ast}}$. Among internal quality factors, vitamin C content increased with decreasing $NO_3$ content and their correlation coefficient was high (r = $-0.795^{{\ast}{\ast}}$). Consequently, $NO_3$ content of kale leaf could be inferred from an external nondestructive method, such as the relative concentration of chlorophyll. We may be able to produce high quality kale leaves containing high amount of vitamin C and low content of $NO_3$ using this method.

Influence of Light Intensity on Plug Establishment of Zoysia-82, Chewings fescue and Red fescue (Zoysia-82, Highlight Chewings fescue 및 Pennlawn Red fescue의 plugging에 대(對)한 조도(照度)의 영향(影響))

  • Kim, In Seob;Kim, Dal Ung
    • Current Research on Agriculture and Life Sciences
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    • v.6
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    • pp.19-24
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    • 1988
  • The present experiment was undertaken to study the influence of light intensity on plug establishment of zoysia-82, chewings fescue and red fescue. The results obtained are summarized as follows : In chewings fescue and zoysia-82, plant height and leaf length were increased and leaf width was decreased by reducing light intensity. But in red fescue, plant height and leaf length measured at 90 and 120 days after plugging under 9% of incident light were shorter than those under 30% of incident light. Percent ground cover was decreased by reducing light intensity in chewings fescue, red fescue and zoysia-82. Each part of dry weight in three species was getting lighter by reducing light intensity. Decrease of light intensity in zoysia-82 causes increasing chlorophyll a, b, and total chlorophyll content. But red fescue and chewings fescue had the most plentiful chlorophyll under 30% of incident light. Chewings fescue and red fescue had the lowest concentration of chlorophyll a/b under natural light intensity. But zoysia-82 had the lowest concentration of chlorophyll a/b under 30% of incident light. Tillering number of chewings fescue and red fescue was decreased by reducing light intensity. Internode length of zoysia-82 was longer in the order of : 1) 30% of incident light, 2) natural light, 3) 9% of incident light. And internode diameter was decreased by reducing light intensity.

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Ecophysiological characteristics of Rosa rugosa under different environmental factors

  • Young-Been Kim;Sung-Hwan Yim;Young-Seok Sim;Yeon-Sik Choo
    • Journal of Ecology and Environment
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    • v.47 no.3
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    • pp.85-102
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    • 2023
  • Background: Ecophysiological characteristics of Rosa rugosa were analyzed under different environmental factors from May to October 2022. Photosynthesis, chlorophyll fluorescence, chlorophyll content, leaf water content (LWC), osmolality, carbohydrate content, and total ion content were measured to compare the physiological characteristics of R. rugosa at two study sites (i.e., in large pots and in the Goraebul coastal sand dune area). Results: When R. rugosa was exposed to high temperatures, photosynthetic parameters including net photosynthetic rate (PN) and stomatal conductance (gs) in both experiment areas declined. In addition, severe photoinhibition occurs when R. rugosa is continuously exposed to high photosynthetically active radiation (PAR), and because of this, relatively low Y(II) (i.e., the quantum yield of photochemical energy conversion in photosystem II [PSII]) and high Y(NO) (i.e., the quantum yield of non-regulated, non-photochemical energy loss in PSII) in the R. rugosa of the pot were observed. As the high Y(NPQ) (i.e., the quantum yield of regulated non-photochemical energy loss in PSII) of R. rugosa in the coastal sand dune, they dissipated the excessed photon energy through the non-photochemical quenching (NPQ) mechanism when they were exposed to relatively low PAR and low temperature. Rosa rugosa in the coastal sand dune has higher chlorophyll a and carotenoid content. The high chlorophyll a + b and low chlorophyll a/b ratios seemed to optimize light absorption in response to low PAR. High carotenoid content played an important role in NPQ. As a part of the osmotic regulation in response to low LWCs, R. rugosa exposed to high temperatures and continuously high PAR used soluble carbohydrates and ions to maintain high osmolality. Conclusions: We found that Fv/Fm was lower in the potted plants than in the coastal sand dune plants, indicating the vulnerability of R. rugosa to high temperatures and PAR levels. We expect that the suitable habitat range for R. rugosa will shrink and move to north under climate change conditions.

Effects of Light Intensity on Photosynthesis and Growth in Seedling of Kalopanax pictus Nakai (광강도에 따른 음나무 유묘의 생장 및 광합성 특성)

  • Lee, Cheul-Ho;Shin, Chang-Ho;Kim, Kyu-Sick;Choi, Myung-Suk
    • Korean Journal of Medicinal Crop Science
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    • v.14 no.4
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    • pp.244-249
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    • 2006
  • This study was carried out to determine leaf photosynthetic capacity and growth characteristics of Kalopanax picturs Nakai seedlings under the different light condition. The seedlings grown under low light condition showed larger leaf area and petiole, and higher relative growth rate than those grown under high light condition. Chlorophyll contents as chlorophyll a, chlorophyll b, and chlorophyll a + b were high in the seedlings grown under highlight condition compared to those grown in low light conditions. The mean absorption value of shade leaf within photosynthetically active radiation (400-700 nm) was slightly higher than that of sun leaf, Leaf photosynthetic capacity of seedling was variable under the different light conditions. Seedling grown under high light condition had the higher photosynthetic capacity. Leaf photosynthetic rates under forestry and nursery were 700 and $300\;{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, respectively. However, leaf photosynthetic rates under high and low light conditions were 500 and $300\;{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, respectively.

Identification and Growth Inhibition of Phytotoxic Substances from Artemisia scoparia (바쑥의 독성물질 확인과 생장억제작용)

  • Kil, Bong-Seop;Hyeon-Gyeong Yoo
    • The Korean Journal of Ecology
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    • v.19 no.4
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    • pp.295-304
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    • 1996
  • KDICical substances from Artemisia scoparia were analyzed by gas chromatography. Seven phenolic compounds and thirty nine terpenoids were identified. Most abundant within each group were cinnamic acid and camphor, respectively. The KDICicals were prepared as aqueous extracts and then used for germination, growth, and chlorophyll content tests. The extracts were inhibitory to germination and seedling growth of the receptor lants. This inhibitory effect was dependent on concentration. When the effect of the aqueous extract on chlorophyll content was assayed, both chlorophylls a and b were shown to be reduced. The reduction in seedling elongation and growth in dry weight paralleled the reduction in chlorophyll concentration. These KDICical substances, including phenolic compounds and terpenoids, from Artemisia scoparia were responsible for the growth inhibition of the selected species.

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Bioaccumulation Patterns and Ecophysiological Responses of Monochoria korsakowi Exposed to Cadmium

  • Lim, Yang-Hoan;Kim, In-Sung;Shim, Hyo-Jung;Kang, Kyung-Hong;Lee, Eun-Ju
    • Animal cells and systems
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    • v.10 no.3
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    • pp.125-130
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    • 2006
  • We have examined the bioaccumulation patterns and the ecophysiological responses (photosynthetic pigment and total antioxidative capacity) of Monochoria korsakowi exposed to various cadmium concentrations, one of major environmental pollutants. Cadmium ion contents in M. korsakowi increased significantly with higher cadmium concentration, and most of the accumulated cadmium was found in the root parts. Biomass of each part decreased with higher cadmium concentration. As cadmium treatment concentration was increased, chlorophyll a content was decreased, whereas chlorophyll b content was increased. However, the variations of total chlorophyll and carotenoid contents were not evident. Total antioxidative capacity in the leaves of cadmium treated M. korsakowi increased greatly with higher cadmium concentration. We considered these results as indicative of the ability of M. Korsakowi plants to take up cadmium from wetlands.

Contents of Chemical Constituents in Organic Korean Cabbages (유기농 배추의 영양 및 기능성 성분 함량)

  • Seong, Jong-Hwan;Park, Sung-Gyun;Park, Eun-Mi;Kim, Han-Soo;Kim, Dong-Seob;Chung, Hun-Sik
    • Food Science and Preservation
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    • v.13 no.5
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    • pp.655-660
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    • 2006
  • To evaluate the chemical characteristics of organic Korean cabbages, the content of moisture, crude protein, crude lipid, crude ash, dietary fiber, mineral, vitamin C, free sugar, organic acid, chlorophyll and carotenoid were analyzed in organic and nonorganic cabbages. The levels of crude protein, crude lipid, crude ash, soluble dietary fiber, insoluble dietary fiber, minerals(Ca, P, Ee, Mg, S, Zn), ascorbic acid, dehydroascorbic acid, fructose, glucose, sucrose, chlorophyll a, chlorophyll b and carotenoid in organic cabbages were higher than those in nonorganic cabbages. However, the content of moisture, malic acid, citric acid, potassium in organic cabbages were lower than those in nonorganic cabbages.

Effect of Light Quality (Red, Blue) on the Major Components of Hot Pepper Fruit (신미종(辛味種) 고추의 주요(主要) 성분(成分)의 함량(含量)에 미치는 광질(光質) (Red, Blue)의 영향(影響))

  • Kim, Kwang-Soo;Roh, Seung-Moon;Park, Jyung-Rewng
    • Korean Journal of Food Science and Technology
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    • v.11 no.3
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    • pp.162-165
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    • 1979
  • In order to study the red-coloring effects of hot pepper fruit by light treatment during after-ripening period, 'Karak Geumjang No. 2 green hot pepper fruits, Capsicum annuum L., after 30 to 35 days from flowering were harvested and white, red and blue light treatments at the energy level of $40\;{\mu}watt/cm^2/sec$ were given at $25^{\circ}C$. When compared with white light, total chlorophyll content was strikingly decreased by blue light treatment and no difference in the chlorophyll contents between red and white light was observed. The chlorophyll a and b showed a similar decreasing patterns as shown in the case of total chlorophyll. Total carotenoid content was higher in the blue light treatment by 31% than the white light. However, red light decreased the carotenoid condent as compared to the white light treatment. But ${\beta}-carotene$ was not changed by red light as compared to white light. Blue light treatment increased ${\beta}-carotene$ content (0.71 mg%-f.w.) as compared to white light treatment (0.56 mg%-f.w.). Therefore, blue light treatment increased red-coloring responses of hot pepper fruit during after-ripening period. The capsaicin content was slightly increased by blue light and no red light influence was observed.

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