• 한국농림기상학회지
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• 제1권2호
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• pp.103-109
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• 1999

This study was carried out to investigate spectral characterisistics and chlorophyll contents of Pinus koraiensis needles in Gwangju-gun, Kyunggi-do. In spectrum analysis by each age, needle showed difference on 700∼1,100 nm(NIR : near infrared ray). Light reflexibility keeps constant between 1st year needle and 3rd year needle, current year needle's value was higher than others and that was higher for 400∼500 nm and 600∼700 nm at 550 nm. Transmission to needle's age showed the highest result in current year needle. It showed in order of current, 1st, 2nd and 3rd year needle at 550 nm and 750 nm. Especially, current year needles of Pinus koraiensis showed a tendency to change transmission coefficient in NIR. The result of transmission coefficient measurement by each position of slope showed in order of middle slope, upper slope and lower slope. But the gathered needle in middle slope made marked change at NIR. Chlorophyll a content of the upper slope's needles was higher than other 4 positions but a component ratio was lower. In needle of the oppressed tree, Chlorophyll a content was rather low than others. Chlorophyll a content by slope positions, the upper slope's needle showed a highest value(4.48 mg/g) and lower slope was lowest value(1.35 mg/g). Chlorophyll b content also showed a similar tendency to chlorophyll a and chlorophyll(a+b) contents.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2006년도 Proceedings of ISRS 2006 PORSEC Volume I
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• pp.479-482
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• 2006

The absorption coefficient and backscattering properties of phytoplankton were calculated from the Mie theory. Given a simple case that phytoplankton and mineral particles are the only constitutions in seawater, the reflectance $b_b({\lambda})/[a({\lambda})+b_b({\lambda})]$was analyzed. Then the chlorophyll concentrations were estimated from remote sensing OC2 algorithm. The results show that reflectance in short wavelength region is more sensitive to the Chl variation; High mineral concentrations in seawater have significant influence on the reflectance spectrum; the existence of high mineral concentration may result in the mistake in chlorophyll estimation from OC2 algorithm.

• Journal of Photoscience
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• 제9권2호
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• pp.362-363
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• 2002

Since chlorophyll a and bacteriochlorophyll a are asymmetric molecules, an external ligand can coordinate to the central Mg atom either from the chiorin macrocycle side where the C13$^2$-methoxycarbonyl moiety protrudes (denoting as the 'back' side) or frome the other side (the 'face' side). We investigated which side of the macrocycle is favored for the ligand coordination, by survey of the highly resolved crystal structures of various photosynthetic proteins and theoretical model calculations. It is found that chlorophyll a as well as bacteriochlorophyll a and b in the photosynthetic proteins mostly bind their ligands on the 'back' sides. This finding was confirmed by the theoretical calculations for methyl chlorophyllide a and methyl bacteriochlorophyllide a as models: the 'back' type ligand-(bacterio )chlorophyll complex was more stable than the 'face' type one. The calculations predicted influence of the Cl3$^2$-stereochemistry on the choice of the side of the ligand coordination, which is discussed in relation to the presence of the Cl3$^2$-epimer of chlorophyll a in photosystem I reaction center [I].

• 한국연초학회지
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• 제21권2호
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• pp.152-159
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• 1999

A CAB cDNA clone(pKGCAB) encoding the light harvesting chlorophyll a/b binding protein of the semi-shade plant, Korean ginseng(Panax ginseng C. A. Meyer) was isolated by the one-way path random sequencing of ginseng cDNA library clones and transgenic tobacco plants(Nicotiana tabacum NC82) were produced by the transformation of this ginseng CAB gene in use of Agrobacterium tumefaciens LBA4404. The CAB gene showed type 1 structure of LHCP-II, 84% similarity in nucleotide sequence and 92% in amino acid sequence to that of Nicotiana tabacum CAB40, respectively. Seed germination and initial growth of the transgenic tobacco plants transformed with the cDNA fragment were accelerated under low light intensity compared with those of normal tobacco plant, that may result from the higher light sensitivity of the transgenic plants than that of the normal.

• 동아시아식생활학회지
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• 제13권6호
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• pp.586-592
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• 2003

본 연구는 국내에서 시판되고 있는 몇 가지 품질의 말차를 이용하여 물의 온도와 교반 시간에 따른 크로로필 함량과 클로로필 패턴을 분석한 것으로서 그 결과를 요약하면 다음과 같다. 1. 한일 말차 각 3종류에 대한 클로로필 함량은 일본산 중품(S5 ; 8.34mg/g), 하품(S6 ; 8.23mg/g)이 가장 많았고 한국산 중품(S2 ; 4.87mg/g)이 가장 적었다. 2. 물의 온도에 대하여는 한국산 말차 중ㆍ하품(S2와 S3)을 제외하고는 85$^{\circ}C$와 95$^{\circ}C$에서 클로로필 함량이 가장 많았다. 3. 교반 시간에 따른 클로로필 함량은 일본산 말차 상ㆍ중ㆍ하(S4 ; 7.87mg/g, S5 ; 8.27mg/g, S6 ; 8.08mg/g)은 60초 교반이 가장 많은 반면 한국산 말차 상품과 하품(S1 ; 6.72mg/g, S3 ; 5.02mg/g)은 15초 교반이 많았다. 4. 클로로필 패턴에 있어서는 페오포비드, 클로로필 a, b 페오피틴 a, b의 5종류의 peak를 확인하였고, 일본산 상품 말차(S4)가 한국산 상품 말차(S1)보다 클로로필 a의 함량이 약 10% 많았다. 그러나 페오피틴 a는 S1이 S4보다 약 20% 많이 함유하고 있었다. 5. 한국산 말차 중 클로로필 패턴의 비율을 보면 상푸(S1)의 클로로필 a의 비율이 47.42%로서 가장 많은 반면 중품(S2)은 30.70%, S2는 23.20%, S3는 37.68%로서 품질간에 뚜렷한 차이를 나타내었다. 일본산 말차의 경우, 클로로필 a는 상품(S4)이 51.57%, 중품(S5)이 63.51%, 하품(S6)이 63.89%이었고 페오피틴 a는 S4, S5, S6가 각각 14.90%, 14.21%, 13,26%로서 한국산 말차에 비하여 뚜렷한 차이는 볼 수 없었다.

• Applied Biological Chemistry
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• 제12권
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• pp.107-114
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• 1969

생장조절제(生長調節劑) GA. IAA, Kinetin, Uracil 및 MH로 Nicotiana Tobaccum var. Virginica를 처리(處理)하여 황변현상(黃變現象)을 관찰(觀察)하여 다음과 같은 결과를 얻었다. 1) 엽연초엽황변시(葉煙草葉黃變時) Chlorophyll 감소(減少)에 따라 Protein 및 RNA의 감소(減少)도 병행(倂行)하였다. 2) GA, IAA, 를 처리(處理)한 엽(葉)은 Protein 과 RNA 의 감소(減少)가 억제(抑制)되고 MA로 처리(處理)된 엽(葉)은 오히려 무처리엽(無處理葉)봐 그들 성분(成分)의 감소(減少)가 심(甚)하였다. 3) 연초엽황변시(煙草葉黃變時) Chlorophyll a/b 비율(比率)의 변동(變動)과 protein 및 RAN 함량(含量)의 변동(變動)과는 아무런 상관관계(相關關係)가 없는것 같았다.

• 생물환경조절학회지
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• 제17권2호
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• pp.101-109
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• 2008

인위적인 토양내 수분처리 결과 물푸레나무와 들메나무 모두 6월<8월<9월로 갈수록 엽록소 함량이 증가하였다. 생육기간이 지나는 동안 엽록소 a는 0.89mg/g에서 1.67mg/g로, 엽록소 b는 0.18mg/g에서 0.44mg/g을 나타냈으며, 두 수종 모두 6월보다 8월과 9월에 높은 함량 증가를 보였다. 두 수종의 광합성율을 보면, 물푸레나무는 각 토양수분 처리구마다 광합성율의 차이를 보였으며, 충분한 토양수분처리구($90{\sim}78%$)의 묘목이 상대적으로 낮은 토양수분조건에서 자란 묘목보다 높은 경향을 나타냈다. 들메나무 역시 물푸레나무와 비슷한 경향을 보였으며, 토양수분이 높을수록 광합성률이 높게 나타났고, 감소할수록 광합성률이 감소하였다. 광합성율의 생육시기별 변화는 두 수종 모두 9월>8월>6월 순으로. 9월에 상대적으로 높은 광합성률을 나타냈다. 이로 미루어보면 두 수종은 생장시기에 상관없이 수분함량이 높은 처리구에서 광합성이 활발히 이루어지는 것으로 생각된다.

• 대한원격탐사학회지
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• 제18권1호
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• pp.35-42
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• 2002

The results of our observation in May 2000 indicated that the SeaWiFS algorithm (O'Reilley et al., 1998), which was adopted for OSMI data processing, overestimated the actual chlorophyll values. This was rather unexpected in that there were good reasons to expect that the bio-optical properties of East/Japan Sea belonged to Case 1 water and in such case, the OC2 algorithm would give unbiased estimates of actual chlorophyll a values. In November 2000, a cruise conducted bio-optical surveys in the same area. This time we added HPLC (High Performance Liquid Chromatography) method for measuring chlorophyll a concentration to the standard fluorometric method, which we hale been using during the past Fluorometric method with acidification is known to result in under/overestimation of chlorophyll values in many parts of the world oceans, while it is easier and cheaper than HPLC method. To our surprise, the comparison of HPLC chlorophyll and fluorometric chlorophyll values show that fluorometric values gave an underestimation up to 50%. This error was due to the presence of accessory pigments such as chlorophyll b. Considering this error, our precious result of May 2000(Yoo et al., 2000) might have to be reinterpreted. Calculation of reflectance at 490 and 555nm, however, indicated that this is not still enough to explain the discrepancies.

• 한국식품과학회지
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• 제10권1호
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• pp.8-10
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• 1978

고추의 Polyethylene Film house 재배시에 피복 재료로서 White, Blue, Green 및 Red Color의 Film을 사용하여 광질에 따른 실험을 행한 바 있다. White와 Red Film의 처리시에 Chlorophyll, Carotenoid, 및 Capsaicin 함량을 측정한 결과는 다음과 같다. 1. 총(總) Chlorophyll의 함량은 White구가 $208.9{\mu}g/g$-F.W., Red구가 $153.0{\mu}g/g$-F.W.으로서 White구에서 그 함량이 현저히 높았으며, Chlorophyll a,b의 비는 White, Red구가 각각 2.15, 2.13으로 비슷하였다. 2. 총(總) Carotenoid는 Red구가 White구의 3.4배에 달하였으며, 특히 $\beta$-carotene은 Red, White구가 각각 $490.6{\mu}g%,\;75.5{\mu}g%$로서 Red구에서 현저히 많았다. 3. Capsaicin의 함량은 Red구가 White구보다 약 20%정도 높았다.

• Journal of Plant Biology
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• 제39권4호
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• pp.309-314
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• 1996

Developmental of photosynthetic pigments and changes in chlorophyll fluorescence of dark-grown rice seedlings were studied during greening. Light-illumination stimulated accumulations of total chlorophylls and carotenoids in leaves of etiolated seedlings, accompanied by a decrease in the ratio of chlorophyll a to chlorophyll b. When the composition of carotenoids was analyzed, violaxanthin level was shown to increase up to 24 h after the beginning of light illumination, followed by a subsequent decline. In contrast to this, zeaxanthin level increased consistently with progress of deetiolatin. The role of zeaxanthin is discussed in relation to chlorophyll fluorescence quenching. A study on chlorophyll fluorescence kinetics of the rice seedlings being deetiolated showed a time-dependent increase in Fv/Fm (yield of variable fluorescence/maximum yield of fluoresecnece) ratios, indicating that greening is responsible for the activation of photochemical reaction centers of the photosystem. When chlorophyll fluorescence quenching was examined, qNP (nonphotochemical quenching) and qE (energy-dependent quenching) exhibited a time-dependent decline with progress of greening. The presented results indicate that greening-induced development of the photosynthetic machinery is associated the conversion of the carotenoid violaxanthin to zeaxanthin, suggesting that zeaxanthin synthesized in the illuminated leaves may provide the protection from the damage when etiolated plants are exposed to light.