• Journal of the Korean Chemical Society
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• v.26 no.5
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• pp.304-309
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• 1982

The fluorescence yields of chl-a and-b complexes bound to silicon dimer, tetramer and hexamer containing pyridine group in diethyl ether solvent, were shown the transition of excited energy through silicon polymer chain, and the maximum energy quenching were appeared at 1 : 1 ratio of chl-a and pyridine group in silicon polymer but the chl-b complexes were shown the maximum energy of fluorescene emission at the same ratio.

• Applied Biological Chemistry
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• v.39 no.4
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• pp.293-296
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• 1996

The present study was carried out to investigate the effect of Ni on germination, cell elongation, ${\alpha}-amylase$ activity, contents of chlorophyll and protein in radish were determined in the water culture. As the concentration of Ni was increased in the water culture, germination of radish was 55% by Ni 10 mg/kg and 30% by Ni 20 mg/kg. The ratio of cell elongation injury was 50%, by two days after Ni 20 mg/kg treatment. The injury ratio of ${\alpha}-amylase$ activity was 45% in the same condition and as the time goes on, inhibition of ${\alpha}-amylase$ activity were slightly decreased. Contents of chlorophyll a and b were decreased two days after treatment and chlorophyll a was more inhibited than chlorophyll b. Also changes of the protein contents was slightly decreased. Activity of ${\alpha}-amylase$ was decreased at germination stage, contents of chlorophyll a and b were decreased at growing stage.

• Asian Journal of Turfgrass Science
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• v.4 no.1
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• pp.12-23
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• 1990

The chlorophyll-protein complexes were separated from thylakoid membranes of Spinach oleracea and Zoysia japonica by two gel Systems of LiDodSO4-PAGE and LiDodSo4/Urea- PAGE under nondenaturing conditions. Seven chlorophyll~protein complexes of CPI*, CPI, CPII*. CP47, CP43, CP29 and CPII were fractionated from both S,oleracea and Zjaponica by LiDodSO4-PAGE. CPI, CP47 and CP43 contained more chlorophyll a than chlorophyll b. The patterns of their absorption spectra at room temperature were similliar to that of chlorophyll a, judging by their UV-spedtroscopy. On the other hand, CPII* and CPII contained approximately equim-olar quantities of chlorophyll a and b. Additional five chlorophyll-protein complexes not separated in the LiDodSO4-PAGE system were electrophoretically isolated from both S, oleracea and Zjaponica by LiDodSO4/Urca-PAGE. The chlorophyll-protein complex just above LRCII $\alpha$in the gel appears CCII-RC separeted recently. 23 kDa and 20 kDa cho-protein complexes is probably LHCIa and LHCIb as judged from their molecular weight. Two novel chlorophyll~protein complexes designated "CPI7" and "CPI6" were fractionate by this gel system. Their molecular weights respectively. Although the stoichiometry of their components and their roles in thylakoid membranes are not apparant, It is thought that they are another kinds of LHCI.other kinds of LHCI.

• Korean Journal of Agricultural and Forest Meteorology
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• v.5 no.1
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• pp.18-23
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• 2003

This study was conducted to compare physiological responses of Pinus densiflora, Populus ${\times}$ tomentiglandulosa, Quercus acutissima and Eleutherococcus sessiliflorus exposed to SO$_2$, by measuring photosynthetic pigment contents and SOD activity. Four woody plants were exposed to relatively high SO$_2$ concentration (500 ppb, 800 ppb) for 8h day$^{-1}$ for 7 days in a chamber. Photosynthetic pigment contents in the leaves of four species decreased with increase of SO$_2$ concentration; also chlorophyll a, chlorophyll b and total carotenoid content were significantly different among tree species and treatments. The ratio of chlorophyll b to chlorophyll a of E. sessiliflorus and Q. acutissima increased for 500 ppb treatment but decreased at 800 ppb. This result showed that chlorophyll a was destroyed by 500 ppb SO$_2$ and chlorophyll b by 800 ppb SO$_2$. Therefore, the sensitivity of chlorophyll a to SO$_2$ may be higher than that of chlorophyll b. SOD activity differed significantly between species and treatments. SOD activity of E. sessiliflorus and Q. acutissima increased at 500 ppb but decreased at 800 ppb, but P. densiflora and P ${\times}$tomentiglandulosa maintained high SOD activity at both 500 ppb and 800 ppb. Based on the photosynthetic pigment contents and SOD activity in the leaves of four tree species, the tolerance of P. ${\times}$ tomentiglandulosa to SO$_2$ was the highest of four tree species.

• Journal of Microbiology and Biotechnology
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• v.11 no.2
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• pp.211-216
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• 2001

The growth of Selenastrum capricornutum in culture was investigated as a function of ionic copper concentrations to verify the toxicity and physiological variation. In a $Cu^{++}$ excess culture (E: $Cu^{++}$ of $130{\mu}g/l$), the growth rate (K)(0.32) was lower than that of the control culture (C: $Cu^{++}$ of $0.065{\mu}g/l$) (0.61) after 8 days and the exponential growth rate ($R_E$) was also found to be lower in culture E (1.1) than culture C (2.9). On the contrary, the K of S. capricornutum in trace $Cu^{++}$ culture ($T_1$ and $T_2$, 0.72) after 6 days tended to be more increased than culture C (0.68). From 8 to 14 days of culture, the amounts of chlorophyllls a and b were increased in culture C (chlorophyll a, $106->126{\mu}g/g$ dry wt; chlorophyll b, $158->208{\mu}g/g$ dry wt; chlorophyll b were decreased in culture E (chlorophyll a, $309->235{\mu}g/g$ dry wt; chlorophyll b, $405->352{\mu}g/g$ dry wt). The amounts of chlorophylls in ionic copper trace culture ($T_1$ and $T_2$) [(chl a/b) of $T_1$: $384/620\;{\mu}g/g$ dry wt; (chl a/b) of $T^2$:$320/467{\mu}g/g$ dry wt] were increased more than the culture C($260/387{\mu}g/g$ dry wt). Howeveer, when photosynthetic rates were normalized to the dry weight of algae, the control culture continued to showed higher values than the treated culture ($T_1$). An appropriate amount of ionic copper ($T_1:\;26\;{\mu}g/l$) stimulated the grwoth of S. capricornutum than the ionic copper content of $13\;{\mu}g/l$ ($T_2$), while the excess amount of ionic copper ($130\;{\mu}g/l$) resulted in the highest toxicity to the growth of S. capricornutum.

• Korean Journal of Soil Science and Fertilizer
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• v.6 no.4
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• pp.231-237
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• 1973

Eeffect of temperature (3-day or 7-day treatment under $15^{\circ}C$, $20^{\circ}C$ and $25^{\circ}C$) on chlorophyll, nitrogen and potassium content in the second leaf blade from top of IR667 line (Suwon 213 and 214) and leading local varieties (Jinheung and Paldal) was investigated at the end of nursery, maximum tillering and flowering stage using phytotron. 1. Chlorophyll content was higher in IR667 line than in the local at $25^{\circ}C$ but reversed at $20^{\circ}C$ or $15^{\circ}C$ and chlorophyll a/b value was always high in the local. 2. Chlorophyll content per fresh weight decreased with growth and low temperature effect was greater at the end of nursery and on chlorophyll a than on chlorophyll b. 3. Chlorophyll a/b value increases with the increase of chlorophyll content and the increasing rate of chl. a/b value per chlorophyll increment tends to decrease under unfavorable condition. This decrease is greater in low temperature sensitive IR667 than local varieties. 4. According to chlorophyll retention value IR667 line was weaker at low temperature. 5. The content of total nitrogen or soluble nitrogen (methanol soluble) was decreased by low temperature. 6. Chl. (a+b)/S-N value decreased with growth and seemed not to be greatly affected by temperature and always higher in the local. 7. Potassium content (total or methanol soluble) tends to decrease at low temperature and soluble K increased with chlorophyll content. 8. High yielding ability of IR667 seems to be attributable to its high chlorophyll content at high temperature and easy leaf discoloration by low temperature or by nitrogen depression of IR667 seems to be attributable to the low Chl./S-N value.

• Journal of Environmental Science International
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• v.5 no.5
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• pp.643-656
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• 1996

Various light intensity and light quality were treated to oat seedlings to investigate the effect of light on the chlorophyll accumulation and the formation of chlorophyll-protein complexes. The Increase of total chlorophyll accumulation and Chl (chlorophyll) a/b ratio was promoted under H (high intensity) white light during oat chloroplast development when compared to 1 (low intensity) white light. Also H white light was more effective in the formation of chlorophyll-protein complexes associated with PSI CCI and CCII than L white light. The seedlings grown in various right quality caused little changes in total chiorophyl and ChI-a/b ratio when compared to those grown in L white light. The assembly of LHCII trimer was more affected by L white light treatment in the formation of-chlorophyll-protein complexes than red light treatment. The effect of blue light on the relative composition of chlorophyll-protein complexes was similar to that of L white light. Particualrly, blue light was more effective in the synthesis of LHCII monomer than the other light quality at the early stage of greening. When compared to red light, blue light was more effective the increase of chlorophyll accumulation and Chl a/b ratio than 1ight Quality, and light Quality may be in important factor for the regulation of the organization in the chlorophyll-protein complexes curing greening.

• Food Science and Biotechnology
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• v.16 no.2
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• pp.205-211
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• 2007

This study was designed to investigate the suppressive effect of chlorophyll a on nitric oxide (NO) production and intracellular oxidative stress. In addition, chlorophyll a regulation of nuclear factor (NF) ${\kappa}B$ activation and inducible NO synthase (iNOS) expression were explored as potential mechanisms of NO suppression in a lipopolysaccharide (LPS)-stimulated macrophage cell line. RAW 264.7 murine macrophages were preincubated with various concentrations ($0-10\;{\mu}g/ mL$) of chlorophyll a and stimulated with LPS to induce oxidative stress and inflammatory response. Treatment with chlorophyll a reduced the accumulation of thiobarbituric acid-reactive substances (TBARS), enhancing glutathione level and the activities of antioxidative enzymes including superoxide dismutase, catalase, glutathione peroxidase (GSH-px), and glutathione reductase in LPS-stimulated macrophages compared to LPS-only treated cells. NO production was significantly suppressed in a dose-dependent manner (p<0.05) with an $IC_{50}$ of $12.8\;{\mu}g/mL$. Treatment with chlorophyll a suppressed the levels of iNOS protein and its mRNA expression. The specific DNA binding activities of NFkB on nuclear extracts from chlorophyll a treated cells were significantly suppressed in a dose-dependent manner with an $IC_{50}$ of $10.7\;{\mu}g/mL$. Chlorophyll a ameliorates NO production and iNOS expression through the down-regulation of NFkB activity, which may be mediated by attenuated oxidative stress in RAW 264.7 macrophages.

• Applied Biological Chemistry
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• v.44 no.2
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• pp.73-80
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• 2001

Factors such as temperature $(20,\;60^{\circ}C)$), pH (4.5, 7.0), gaseous phase $(N_2,\;0_2)$, and light (0 lux, 5,000 lux), antioxidants and packaging conditions were investigated to study the effects of above factors on the chlorophyll components in spinach during storage. Regardless of other factors, as the storage temperature increased from $20^{\circ}C$ to $60^{\circ}C$ and pH decreased from 7.0 to 4.5, the contents of chlorophyll a and chlorophyll b in spinach decreased significantly (P<0.05). The amounts of chlorophyll a and chlorophyll b in spinach stored in nitrogen gas were significantly (P<0.05) lower than those in sample in oxygen phase. As the light intensity increased from 0 lux to 5,000 lux during storage, the contents of chlorophyll a and chlorophyll b in spinach significantly (P<0.05) decreased. The antioxidants reduced the degradation of chlorophyll a in a model system during dark storage by minimization of free radical oxidation. The effectiveness of antioxidants decreased as following orders; ${\alpha}-tocopherol$>ascorbic acid>${\beta}-carotene$>catechin>quercetin>rutin>kaempherol>caffeic acid>chlorogenic acid>p-coumaric acid>ferulic acid. The degradation of chlorophyll a in a model system during light storage was minimized by antioxidants due to the reduction of singlet oxygen oxidation. The antidiscoloring potential of antioxidants decreased as following orders; ${\beta}-carotene$>${\alpha}-tocopherol$>ascorbic acid>catechin>quercetin>rutin>kaem-pherol>caffeic acid>chlorogenic acid>p-coumaric acid>ferulic acid. The amounts of chlorophyll a and chlorophyll b in freeze dried spinach packed with polyethylene bag were significantly (P<0.05) lower than those in non-packed freeze dried spinach. The package of spinach in polyethylene bag with the combination of antioxidants could be used to minimize the degradation of chlorophyll components in spinach during storage.

• Journal of Korean Society of Forest Science
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• v.76 no.1
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• pp.11-16
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• 1987

With the purpose of pursuing the increase and decrease of chlorophyll a and chlorophyll b caused by application of the simulated acid rain treatment on Pinus koraiensis seedlings and Ligustrum obtusifolium cuttings, the experimental design of randomized block arrangement with three replications was implemented in the experimental field of Yesan National Agricultural Junior College, during the growing season of 1985. Pinus koraiensis seeds stratified in cool and moist condition were sown on pots, and in case of Ligustrum obtusifolium, C1/1 cuttings were potted for experimental use in the early spring. The regime of artificial acid rain, based upon precipitation frequency and density, was simulated from the learning of climatological data averaged from 30 years records. The spray of acid water containing pH values of 4.0 and 2.0 was initiated from the 1st of May and ended on the 31st of August. As control, ground water was also treated at the same time. To analyse the chlorophyll content, those leaves looking representative and unaffected by other harmful agents were sampled on the 18th of September, and UV-visible spectrophotometer was used. With decrease in pH values of acid rain, the content of chlorophyll a and chlorophyll b decreased in both species. The decrease in chlorophyll a could be confirmed through statistical significance, but not in chlorophyll b. And when we discussed the chlorophyll decrease index which was explained in detail in the paper, an attention might be given to similarly decreasing values in both chlorophyll a and chlorophyll b, according as pH levels of acid rain decreased. The ratios of chlorophyll a to chlorophyll b in both species were not affected by different pH leaves of acid rain.