Korean Journal of Agricultural and Forest Meteorology (한국농림기상학회지)

Korean Society of Agricultural and Forest Meteorology (한국농림기상학회)
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Changes of Photosynthetic Pigment Contents and SOD Activity in the Leaves of Four Tree Species Exposed to SO2

SO2 노출된 4개 수종의 엽내 광색소 함량 및 SOD 활성 변화

  • Published : 2003.03.01
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Abstract

This study was conducted to compare physiological responses of Pinus densiflora, Populus ${\times}$ tomentiglandulosa, Quercus acutissima and Eleutherococcus sessiliflorus exposed to SO$_2$, by measuring photosynthetic pigment contents and SOD activity. Four woody plants were exposed to relatively high SO$_2$ concentration (500 ppb, 800 ppb) for 8h day$^{-1}$ for 7 days in a chamber. Photosynthetic pigment contents in the leaves of four species decreased with increase of SO$_2$ concentration; also chlorophyll a, chlorophyll b and total carotenoid content were significantly different among tree species and treatments. The ratio of chlorophyll b to chlorophyll a of E. sessiliflorus and Q. acutissima increased for 500 ppb treatment but decreased at 800 ppb. This result showed that chlorophyll a was destroyed by 500 ppb SO$_2$ and chlorophyll b by 800 ppb SO$_2$. Therefore, the sensitivity of chlorophyll a to SO$_2$ may be higher than that of chlorophyll b. SOD activity differed significantly between species and treatments. SOD activity of E. sessiliflorus and Q. acutissima increased at 500 ppb but decreased at 800 ppb, but P. densiflora and P ${\times}$tomentiglandulosa maintained high SOD activity at both 500 ppb and 800 ppb. Based on the photosynthetic pigment contents and SOD activity in the leaves of four tree species, the tolerance of P. ${\times}$ tomentiglandulosa to SO$_2$ was the highest of four tree species.

목본 식물을 대상으로 SO$_2$에 의해 나타나는 생리적인 반응을 구명하기 위하여 소나무, 오갈피나무, 현사시, 상수리나무를 대상으로 SO$_2$를 500 ppb, 800 ppb로 하루에 8시간씩 7일간 처리하여 잎의 광색소 함량과 SOD 활성을 비교 분석한 결과는 다음과 같았다. SO$_2$의 처리 농도가 증가할수록 4개 수목의 엽 내 엽록소 함량은 감소하였으며, 엽록소 a와 엽록소 b, 카로티노이드 함량의 변화는 수종별, 처리별로 다른 경향을 보였다. 오갈피나무와 상수리나무의 엽록소 b와 a의 비는 SO$_2$는 500 ppb 처리구에서는 증가하다가 800ppb 처리구에서는 감소하였다. 즉 500ppb에서는 엽록소 a가 파괴되며, 800ppb에서는 엽록소 b도 파괴될 수 있음을 보여 주었으며, SO$_2$에 대한 민감성은 엽록소 a가 엽록소 b보다 높은 것으로 나타났다. 4개 수종의 잎 SOD활성은 수종별, 처리별 큰 차이를 나타냈다. 오갈피나무와 상수리나무는 500 ppb 처리구에서는 SOD 활성이 증가하다가 더 높은 농도에서는 활성이 감소하였으며, 소나무와 현사시의 경우는 500ppb 처리와 800ppb 처리에서 높은 SOD활성을 유지하여 내성을 보인다. 그러나 광색소와 SOD 활성을 기준으로 판단해 볼 때, SO$_2$에 대한 저항성은 현사시가 가장 높은 것으로 판단된다.

Keywords

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