• Korean Journal of Environment and Ecology
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• 제22권5호
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• pp.595-597
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• 2008

We photographed a nest of Bubo bubo kiautschensis in Icheon, Gyeonggi-do, which had laid 3 eggs and was incubating the eggs. Although 2 chicks were hatched from the 2 eggs, one of them disappeared and the other of them died in March 8, 2007. After that, the parent bird ate the dead chick and left the nest abandoning breeding the next day.

• Applied Microscopy
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• 제18권2호
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• pp.119-131
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• 1988

The purpose of this study was to investigate the differentiation and degeneration of neurons in developing chick embryo. The activity of acid phosphatase(ACP) was measured and cytochemical study of ACP and ultrastructural changes were observed in prosencephalon, mesencephalon and rhombencephalon from day 4 to day 19 of incubation. As a result, the activity of ACP of all brain region was tend to increase from day 4 to day 19. On day 13, activities of ACP of mesencephalon and rhombencephalon were increased greatly and activity of ACP was decreased each region on day 17. On electron microscopic examination, the reaction product of ACP were localized at GERL complex, lysosome, Golgi body and vacuoles of neurons. Morphologically, disrupted nuclear envelope, mitochondrial destruction, vacuolization and ribosomal crystalization were observed.

• Biomolecules & Therapeutics
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• 제15권2호
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• pp.89-94
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• 2007

Ginseng is a widely-used alternative medicine for the treatment of cancer, diabetes, and cardiovascular diseases. Active components of P. ginseng, absorbed through gastrointestinal tract are the fermented ginsenosides by intestinal microorganisms. In the present study, we investigated the inhibitory effects of fermented ginseng with bifidobacterium (FGb) on the angiogenesis by analyzing in vitro tube formation and invasion assay using human umbilical vein endothelial cells (HUVECs), and in vivo angiogenesis using chick chorioallantoic membrane (CAM) assay. Treatment with FGb inhibited tube-like structure formation in a concentration-dependent manner. In addition, FGb significantly suppressed HUVEC invasion through Matrigel. Moreover, FGb dosedependently inhibited VEGF-induced angiogenesis in a CAM assay. These results suggest that FGb is a valuable anti-angiogenic remedy.

• Asian-Australasian Journal of Animal Sciences
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• 제7권4호
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• pp.499-503
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• 1994

One thousand and eighty eggs of Japanese quail (Coturnix coturnix Japonica) were set into the incubator maintaining 36 treatment groups (3 egg weight groups ${\times}3$ seasons ${\times}4$ preincubation holding periods) to evaluate their hatchability performances. Holding periods had significant (p<0.05) effect on hatchability and the seasons showed significant (p<0.05) effect on chick weight. All the parameters (except fertility) were significantly (p<0.01) influenced by the egg weight. None of the parameters maintained regular trend with egg weight and pre-incubation holding periods. Significant interactions were not observed on any of the parameters (except fertility) studied. The egg weight maintained significant (p<0.05) negative correlation with fertility and positive correlation with chick weight. Eggs of medium weight (9.10 to 10.00 cm) could be hatched satisfactorily between 4 and 7 days of pre-incubation holding periods in any season of the year.

• Journal of the Korean Society of Food Science and Nutrition
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• 제19권4호
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• pp.335-341
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• 1990

Nuclei proteins were purified from chick liver to homogeneity by means of acid extraction CM Sephadex c 25 column chromatography and Bio Rex 70 column chromatography, The molecular weight of liver Nuclei proteins 1 and 2 as estimated by electrophoresis on SDS-polycrylamide gel are 29000 and 27,000 respectively. These molecular weights are identical with those of Nuclei Proteins 1 and 2 isolated from chick erythrocyte. The liver and erythrocyte Nuclei Proteins also co-migrated in acetic acid-urea gel electrophoresis. Furthermore the anti-sera raised against liver Nuclei Proteins 1 and 2 cross-reacted with erythrocyte Nuclei Proteins 1 and 2 respectively, However the amino acid compositions of liver Nuclei Prooteins 1 and 2 were found to be different from those of corresponding erythrocyte Nuclei proteins ; the contents of serine and proline in liver Nuclei proteins were higherocyte Nuclei proteins ; the contents of serine and proline in liver Nuclei protesins were higher than those in erythrocyte Nuclei proteins while the content of lycsine in liver Nuclei proteins was lower than the erythrocyte Nuclei proteins, These results suggest that in spite of similarities in many respects the liver and erythrocyte Nuclei proteins in chicks and different proteins.

• Korean Journal of Veterinary Service
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• 제21권4호
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• pp.407-411
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• 1998

Aspergillosis, caused by infection with Aspergillus fumigatus and less commonly by other Aspergillus species, is a prevalent and costly respiratory disease of poultry, In a flock of chicks, the number of birds, 4∼5 months old, had become gradually emaciated and subsequently died. Gross necropsy revealed multiple granulomatous masses on the abdominal serosa. The masses, 4∼15 mm in size, were attached on mainly intestinal wall. Also, the smaller masses in size were on mesentery and pancreas. However, only a few small white nodules were scattered throughout liver and lung in few samples. Microscopically, the mass were granulomatous with a central area of necrosis containing numerous septate, branched fungal hypae consistent with Aspergillus sp. These were surrounded by macrophages, giant cells, lymphocytes and fibrous tissues. Nodular lesions of liver and lung were seemed to spread hemotogenously from intestine and the possible route of infection was speculated by oral. This report is a Aspergillus-induced granuloma limited to the serosa of abdominal cavity, especially of intestinal wall.

• The Korean Journal of Zoology
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• 제37권3호
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• pp.428-438
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• 1994

HgC12의 투여량에 따른 8일 계배의 대뇌 신경세포 분화에 미치는 영향을 규명하기 위하여 신경세포의 미세구조 변화를 전자현미경을 이용하여 관찰하였으며, 또한 탈수소효소의 활성도 및 ATP의 변화 상을 분석한 결과는 다음과 같다. 대뇌 신경세포의 미세구조의 변화는 0.1mg과 0.5mg 투여 군에서는 핵막이 약간 불규칙하게 관찰되지만 조면소포체와 Golgi복합체가 발달되고 rnltochondria가 다수 관찰되었다 그러나 1.Omg 투여군에서는 조면소포체와 Golgi복합체의 발달이 미약하였으며. 2.Omg 투여군에서는 핵막이 파괴되고 2중막이 분리되었으며, Golgi복합체의 발달이 매우 미약하였다. 탈수소효소 촬성도는 수은의 투여량이 증가할수록 감소하는 현상을 보였는데 LDH의 활성도는 1.Omg투여군이 약간 감소하였고, 2 Omg 투여 군에서는 69n로 크게 감소 현상을 보였다. MDH활성도는 2.Omg투여 군에서 82%로 활성이 감소하는 것으로 나타났다 한편 SDH활성도는 1.Omg투여 군에서 80%. 2.Omg투여군에서는 65%로 활성이 크게 감소하였다. ATP의 변화는 1.Omg투여군 이하에서는 정상군과 유의한 변화는 없으나 2.Omg투여군에서는 크게 증가하는 것으로 나타났다.

• The Korean Journal of Zoology
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• 제35권2호
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• pp.144-148
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• 1992

Insulin and transfenin (Tf) were found to be essential for suwival and differentiation of brain neuroblasts obtained from chick embryo. This requirement, however, is Changed from insulin to Tf upon neuronal development of the embryo, and this phenomenon is due to the changes in the levels of corresponding receptors. Using cultured neuroblasts, the level of Tf receptor is also found to increase Lvhile that of insulin receptor falls dramatically during the course of the cell differentiation. These results suggest that the development-specific changes in the levels of insulin and Tf receptors in embryo can be reproduced in the culture system during the differentiation period. Because the culture system used was a defined medium and contained no other macromolecules than insulin and Tf, it appears possible that insulin and Tf may act as signalling molecules in the control of neuronal development.

• The Korean Journal of Zoology
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• 제34권4호
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• pp.610-617
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• 1991

계배 근원세포의 분화 및 증식에 미치는 F3+의 영향을 조사하였다. 철이 없는 배양액은 근원 세포의 분화와 증식을 억제하는 것으로 나타났으며 , 따라서 근원세포의 분화에 철과 transferrin (Tf)은 필수적이다. 또한, 철 대신에 Co2+가 부착된 Tf가 첨가된 배양액에서도 근세포의 분화가 정상적으로 일어나는 것으로 나타났다. Lysosomotrophic amine(chloroquine, $\mu$M 수준;ammonium chloride, mM 수준)은 근세포의 분화와 증식을 억제시켰으며 , 근세포 분화의 철에 대한 의존성은 분화됨에 따라 둔화되었다. 세포내로의 T니 수송량은 MEM과 8102배양액에서 비슷하였고, 근세포가 분화됨에 따라 감소하였다. Lysosomotrophic azine은 최소한 3시간이 내에서는 세포내로 수송되는 Tf의 양에 영향을 미치지 못하였다.

• The Korean Journal of Zoology
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• 제26권1호
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• pp.1-17
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• 1983

The synthesis of myosin, actin, tropomyosin and troponin in the cultured muscle cells of chick embryo during the differentiation were analyzed. The synthesis of myosin and actin were very active prior to the myoblast fusion while the troponin synthesis became active after the fusion. Tropomyosin was synthesized practically constantly throughout the culture period. Several proteins were detected in the muscle-conditioned medium strongly suggesting that the cells in culture released polypeptides which might act on the membrane of neighboring cells cells to initiate the fusion.