• Title/Summary/Keyword: chemotaxonomy

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The Chemical Constituents of Some Taiwanese Liverworts

  • Wu, Chia-Li
    • Korean Journal of Pharmacognosy
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    • v.16 no.4
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    • pp.243-247
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    • 1985
  • Twelve species of Taiwanese liverworts from recent collection were examined by GC/MS and some of the major components were isolated and identified spectroscopically. Interesting findings from the view point of chemotaxonomy are discussed in the paper.

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Chemotaxonomy of Trichoderma spp. Using Mass Spectrometry-Based Metabolite Profiling

  • Kang, Dae-Jung;Kim, Ji-Young;Choi, Jung-Nam;Liu, Kwang-Hyeon;Lee, Choong-Hwan
    • Journal of Microbiology and Biotechnology
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    • v.21 no.1
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    • pp.5-13
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    • 2011
  • In this study, seven Trichoderma species (33 strains) were classified using secondary metabolite profile-based chemotaxonomy. Secondary metabolites were analyzed by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS-MS) and multivariate statistical methods. T. longibrachiatum and T. virens were independently clustered based on both internal transcribed spacer (ITS) sequence and secondary metabolite analyses. T. harzianum formed three subclusters in the ITS-based phylogenetic tree and two subclusters in the metabolitebased dendrogram. In contrast, T. koningii and T. atroviride strains were mixed in one cluster in the phylogenetic tree, whereas T. koningii was grouped in a different subcluster from T. atroviride and T. hamatum in the chemotaxonomic tree. Partial least-squares discriminant analysis (PLS-DA) was applied to determine which metabolites were responsible for the clustering patterns observed for the different Trichoderma strains. The metabolites were hetelidic acid, sorbicillinol, trichodermanone C, giocladic acid, bisorbicillinol, and three unidentified compounds in the comparison of T. virens and T. longibrachiatum; harzianic acid, demethylharzianic acid, homoharzianic acid, and three unidentified compounds in T. harzianum I and II; and koninginin B, E, and D, and six unidentified compounds in T. koningii and T. atroviride. The results of this study demonstrate that secondary metabolite profiling-based chemotaxonomy has distinct advantages relative to ITS-based classification, since it identified new Trichoderma clusters that were not found using the latter approach.

A DEEPLY BRANCHED NOVEL PHYLOTYPE FOUND IN PADDY SOIL

  • Kim, Hong-Ik;Kazunori Nakamura;Hiroshi Oyaizu
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 2000.04a
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    • pp.128-134
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    • 2000
  • In the course of flora analysis of soil Archaea, we found very strange 16S rDNA clones, which could possibly constitute a sister clade from known two archael, Crenarchaeota and Euryarchaeota, lineages. Overall signature sequences showed that the clones were closely related to domains Archaea and Eucarya. However, at least nine nucleotides distinguished the novel clones from domains Archaea and Eucarya. Phylogenetic trees drawn by maximum parsimony, neighbor joining and maximum likelihood methods also showed unique phylogenetic position of the clones. A very specific primer set was synthesized to detect the presence of the novel group of organisms in terrestrial environments. A specific DNA fragment was amplified from all of paddy soil DNAs, and this fact suggests that the novel organisms inhabit paddy soils.

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Numerical Identification of an Actinomycetes Strain Producing an Antitumor Antibiotic with Inhibitory Activity against DNA Topoisomerase (DNA topoisomerase I Inhibitor 를 생성하는 방선균 분리균주의 수리동정)

  • Lee, Dong-Sun;Ha, Sang-Chul;Shin, Woo-Chang;Kim, Tae-Ho;Kim, Hong-Joong;Park, Yong-Ha;Kim, Jong-Guk;Hong, Soon-Duck
    • Microbiology and Biotechnology Letters
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    • v.23 no.2
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    • pp.123-130
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    • 1995
  • DNA topoisomerase I have been shown to be important therapeutic target in cancer chemotherapy. Chemotaxonomy and numerical identification were carried out for an isolate strain No.7489 producing an antibiotic that inhibits DNA topoisomerase I activity. The genus of strain No.7489 was determined as Streptomyces sp. from culture, morphological and chemotaxonomic data. Thirty-nine taxonomic unit characters were tested and the data were analyzed numerically using the TAXON program. The isolate was best matched to Streptomyces melanosporofaciens in the major cluster 32 of Streptomyces. Therefore, it was concluded that the isolate was identified to be a member of Streptomyces melanosporofaciens.

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Correlation between Phylogeny and Zn-Resistance in Methylobacterium Species Isolated from Non-Polluted Soil Environments

  • Kim, Hong-Ik;Kazunori Nakamura;Hiroshi Oyaizu
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 2000.10a
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    • pp.118-124
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    • 2000
  • Zn-resistant Methylosobacterium strains were isolated from several non-polluted soil samples collected in all over Japan. Zn-resistant Methylosobacterium strains were predominantly detected in all soil samples and they were also characterized as a UV-tolerant bacteria. The MIC test revealed that the isolates have high zinc resistance in comparison with that of reference Methylosobacterium strains obtained from culture collections. The 16S rDNA-based phylogenetic analysis showed that all strains were divided into two clusters designated as cluster A and cluster B in the present study. All isolates were distributed only in the cluster A. The phylogenetic clustering also well coincided with the differences in the pattern of carbon source utilization.

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Numerical Identification of an Actiomycetes Stain Producing an Antibiotic that Generates Oxygen Radical (활성산소를 발생하는 항생물질을 생성하는 방선균 분리균주의 수리동정)

  • Lee, Dong-Sun;Ha, Sang-Chul;Shin, Woo-Chang;Kim, Tae-Ho;Kim, Hong-Joong;Park, Yong-Ha;Kim, Jong-Guk;Hong, Soon-Duck
    • Microbiology and Biotechnology Letters
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    • v.23 no.4
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    • pp.397-404
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    • 1995
  • Chemotaxonomy and numerical identification were carried out for an isolate strain No.8001 producing an antibiotic that generates oxygen radical. The genus of strain No.8001 was decided as Streptomyces sp. from chemotaxonomic data. Thirty-nine taxonomic unit characters were tested and the data were analyzed numerically using the TAXON program. The isolate was best matched to Streptomyces citreofluorescens in the major cluster 1B of Streptomyces. Therefore, it was concluded that the isolate was identified to be a member of Streptomyces citreofluorescens.

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A chemosystematic investigation of selected Stichococcus-like organisms (Trebouxiophyta)

  • Van, Anh Tu;Karsten, Ulf;Glaser, Karin
    • ALGAE
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    • v.36 no.2
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    • pp.123-135
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    • 2021
  • The taxonomy of green microalgae relies traditionally on morphological traits but has been rapidly changing since the advent of molecular methods. Stichococcus Nägeli is a cosmopolitan terrestrial algal genus of the class Trebouxiophyceae that has recently been split into seven lineages, which, along with Pseudostichococcus, comprise the Stichococcuslike group; there is a need to further characterize these genera, since they are morphologically enigmatic. Here we used organic osmolytes as chemotaxonomic marker to verify the phylogenetic position of Stichococcus-like strains and were also able to exclude a strain hitherto identified as Gloeotila contorta from this group. Stichococcus-like organisms, including those recently revised, were characterized by the production of the polyol sorbitol and the disaccharide sucrose in high amounts, as is typical of Prasiola-clade algae. The results demonstrate that organic osmolyte chemotaxonomy can support green algal taxonomic designations as fundamental research.