• Natural Product Sciences
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• 제27권2호
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• pp.122-127
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• 2021

A new steroidal saponin, (23S,24S)-spirosta-5,25(27)-diene-1𝛽,3𝛽,23,24-tetrol 1-O-((2,3-diacetyl-α-L-rhamnopyranosyl)-(1→2)-[𝛽-D-xylopyranosyl-(1→3)]-α-L-arabinopyranoside)-24-O-𝛽-D-glucopyranoside (humilisoside) together with the known 𝛽-sitosterol 3-O-glucopyranoside, adenosine, dioscin, and methylprotodioscin were isolated from the leaves of Dracaena humilis. Their structures were elucidated by spectral techniques including mass spectrometry (ESIMS, HRESIMS, tandem MS-MS), 1D NMR (¹H, ¹³C NMR), 2D NMR (HSQC, ¹H-¹H COSY, HMBC, NOESY), chemical method as well as by comparison with spectroscopic data reported in the literature. The chemotaxonomic significance of the isolation of these compounds is discussed. This is the first report on the phytochemical investigation of D. humilis.

• Journal of Forest and Environmental Science
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• 제40권2호
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• pp.123-140
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• 2024

More than half of the global distribution of the Rhododendron genus is found in China, with over 74% being endemic species. However, there is still insufficient data to chemically classify the Rhododendron genus native to China. Therefore, in this study, a chemotaxonomic study was conducted to determine the presence of taxifolin-3-O-arabinopyranoside, a flavonoid compound, in the Rhododendron genus native to China. Forty-three species of Rhododendron native to China-20 from across China, 8 from Beijing, 6 from Yanbian, and 9 from Yunnan-were utilized in the experiment. Through HPLC analysis, the retention time was compared with that of taxifolin-3-O-arabinopyranoside, a standard compound, and quantitative analysis was conducted. As a result, taxifolin-3-O-arabinopyranoside was detected in 22 out of 43 the Chinese Rhododendron species. Afterwards, LC-MS/MS analysis was performed on the 22 species in which taxifolin-3-O-arabinopyranoside was detected to determine whether the molecular weight was consistent with the standard compound. Under negative conditions, it was confirmed that all samples exhibited the same molecular weight as taxifolin-3-O-arabinopyranoside, 435-436 m/z. The same compound was detected in more than half of the Rhododendron species used in the experiment, and taxifolin-3-O-arabinopyranoside was determined to be an indicator compound for Rhododendron species native to China. In addition, the possibility of using the above results as basic data for chemical classification of Chinese Rhododendron genus was confirmed.

• Journal of Forest and Environmental Science
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• 제38권3호
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• pp.159-173
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• 2022

Genus of Rhododendron has been used in traditional medicine since ancient times and is known to be effective in immune function, inflammation, and cold symptoms. And the reason for this activity is the flavanonol type among flavonoids in the genus of Rhododendron. Among the flavanonol types, Taxifolin-3-O-arabinopyranoside was isolated from the root of native R. mucronulatum in Korea, and the structure was finally identified through HPLC, LC-MS/MS, ¹H-NMR, and ¹³C-NMR. Taxifolin-3-O-arabinopyranoside is a compound mainly found in R. mucronulatum, a representative species of the genus of Rhododendron, and exhibits antioxidant, anti-inflammatory, and anti-atopic activities. In this study, Taxifolin-3-O-arabinopyranoside was chemotaxonomic significant in 5 species of the genus Rhododendron native to Korea (R. mucronulatum, R. mucronulatum var. ciliatum, R. schlippenbachii, R. yedoense var. Poukhanense, R. japonicum for. Flavum). Compared with the existing literature, Taxifolin-3-O-arabinopyranoside was identified for the first time in 4 species of Rhododendron except for the R. mucronulatum.

• Journal of the Korean Wood Science and Technology
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• 제48권6호
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• pp.888-895
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• 2020

Ulmus genus has excellent various physiological activities, including anti-ulcer, antioxidant, antibacterial, anti-cancer, immunity, and homeostasis maintenance effects, and it is known to have many additional drug effects And one of reasons for these excellentbiological activities is a flavan-3-ol chemical group in Ulmus genus. In this study a new flavan-3-ol compound was identified in Ulmus davidiana var. japonica. A flavan-3-ol,(2R,3S)-7-[(2S,3R,4R)-3,4-dihydroxy-4-(hydroxymethyl)oxolan-2-yl]oxy-2-(3,4-dihydroxyphenyl)-3,4-dihydro-2H-chromene-3,5-diol, named as catechin 7-O-beta-D apiofuranoside, was isolated from the stems and barks of Ulmus davidiana var. japonica for. suberosa, which is a species belonging to the genus Ulmus, growing throughout the Korea peninsula. The structure was elucidated by various spectroscopic methods including high-resolution TOF mass spectrometry, ¹H-NMR and ¹³C-NMR spectrometry and comparison with chemical structures of defined compounds.

• 생명과학회지
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• 제8권1호
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• pp.27-31
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• 1998

Isoprenoid quinones은 미생물의 호흡계나 관합성의 전자전달계의 필수적인 구성성분으로, 그들의 화학적분류 뿐만 아니라 생리학적인 중요성으니 충분히 인식되어 있다. 본실험에서는 Trichoderma, Gliocladium, Verticillium, Aspergullus 및 Agaridus bisporus, 및 Agaricus biporus, Lenrinus edodes, Pleurotus ostrearus igniarius, Phellinus chrysolona를 포함한 일부 식용버섯의 ubiquinone type을 결정하였다. 대부분의 불완전균휴는 Q-10($H_2$)를, 본 실험에 사용된 식용버섯 전체는 Q-9을 main ubipuinone type을 가지는 것으로 나타났다. 따라서 upiquinone type은 진균류에 있어서도 화학적 분류의 유용한 수단으로 간주되며, 보다 광범위한 연구결과의 축척이 필요한 것이다.

• Journal of Forest and Environmental Science
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• 제40권1호
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• pp.53-63
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• 2024

Alnus japonica is indigenous to korea and usually grows in wet lowlands and damp regions in the mountains. Oregonin, is known as a representative compound of the diarylheptanoid mainly found in Alnus species and has been reported to have anti-inflammatory and antioxidant effects. In this experiment, we conducted a study on the presence of orogonin in the native Alnus japonica from the Baekdudaegan mountain range in Korea. We collected a total of 30 samples from Chuncheon, Yangyang, and Jeongseon and conducted experiments. In the HPLC analysis, we confirmed that oregonin was detected in all samples. After the quantitative analysis, samples with the highest content from each region were selected and LC-MS/MS experiments were performed. As a result, the same molecular weight as oregonin, 477.2 m/z, was confirmed in all samples.

• Toxicological Research
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• 제23권1호
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• pp.79-86
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• 2007

The isolated plantamajoside from Plantago asiatica that is often used as a marker compound in chemotaxonomic studies has various bioactivites such as the inhibitions of cyclic AMP phosphodi-esterase and 5-lipoxygenase, microbial growth and inflammation, and currently demands the generation of toxicity data. The purpose of this study was to examine the toxicities of the single and 14 days repeated dose toxicity in Sprague-Dawley rats orally administrated with plantamajoside at dose levels of 0, 500, 1000, and 2000 mg of dried material/kg body weight/day. The results showed that there was no difference in body weight change, food intake, water consumption, or relative organ weight among different dose groups. Also we observed no death and abnormal clinical signs were observed during the experimental period. Between the groups orally administered Plantago asiatica and the control group, there was no statistical significance in hematological test or serum biochemical values. There were no gross findings at final sacrifice. There was no evidence of histopathological alteration mediated by 14 days treatment with Plantago asiatica. These results suggest that no observed adverse effect level (NOAEL) of the oral application was considered to be more than 2000 mg/kg in rats under the conditions employed in this study. Another observation was performed to investigate the safety of Plantago asiatica in respect of genotoxicity. This substance was examined that Salmonella typhimurium reversion assay (Ames test) in strain TA98, TA100, TA1535. In the reverse mutation test, Plantago asiatica did not induce mutagenicity in Samonella typhimurium with and without metabolic activation. These results indicated that Plantago asiatica had no genotoxicity.