• Title/Summary/Keyword: chemo-enzymatic synthesis

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Hydrogel Synthesis using Glycosyl Methacrylate and Acrylate: 1. A Study on Chemo-Enzymatic Synthesis of Sorbitan Acrylate (배당화 메타크릴레이트와 아크릴에리트를 이용한 하이드로겔의 합성: I. 솔비탄 아크릴레이트의 화학.효소적 합성에 관한 연구)

  • 박돈희;임근길;정귀택;변기영;김인흥;이광연;김해성
    • KSBB Journal
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    • v.18 no.3
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    • pp.222-228
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    • 2003
  • This study was performed to research a chemo-enzymatic synthesis of sorbitan acrylate. It w as firstly to determine the optimum conditions for D-sorbitol cyclic reaction in the presence of p-toluenesulfonic acid (p-TSA) as catalyst material. It was secondly to find the optimum conditions for sorbitan acrylate synthesis using immobilized lipase Novozym 435 in t-butanol from its materials. The maximum yield of 1,4-sorbitan synthesis were obtained approximately 90% (w/w) at 13$0^{\circ}C$ and 200 mmHg vacuum pressure with 1% (w/w) p-TSA after 150 min reactin time on our experimental system. The product from optimum condition was less color than those obtained at higher temperatures and minimized byproduct and unreacted D-sorbitol. Sorbitan acrylate was synthesized to around 63.5% conversion of 1,4-sorbitan. The experimental optimum condition was found at 5$0^{\circ}C$, atmospheric pressure, 3% (w/v) Novozym 435, 50 g/L 1,4-sorbitan of initial reactant concentration, and 1:3 molar ratio of 1,4-sorbitan to acrylic acid.

Enzymatic Synthesis of Sorbitan Methacrylate Effect of Reaction Temoerature and Acyl Donor (솔비탄 메타크릴레이트의 효소적 합성 - 반응온도와 아실 공여체의 영향 -)

  • Jeong Gwi Taek;Park Eun Soo;Byun Ki Young;Lee Hye Jin;Kim In Heung;Joe Yung Il;Kim Hae Sung;Song Yo Soon;Kim Do Heyoung;Ryu Hwa Won;Lee Woo Tae;Sun Woo Chang Shin;Park Don Hee
    • KSBB Journal
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    • v.19 no.5
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    • pp.385-389
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    • 2004
  • In this research, the chemo-enzymatic synthesis of sorbitan methacrylate was investigated to optimize reaction conditions. Firstly, sorbitan was manufactured by sorbitol cyclic reaction in the presence of p-toluenesulfonic acid (p-TSA) as catalyst material. Secondly, sorbitan methacrylate was synthesized by immobilized lipase Novozyme 435 with acyl donors in t-butanol. As a result of enzymatic synthesis of sorbitan methacrylate, the conversion yield reached about $65\%$ in the condition of initial sorbitan conc. 50 g/L, enzyme content $3\%$ (w/v) , molar ratio 1:3, reaction temperature 50^{circ}C and reaction time 42 hrs using methyl methacrylate as acyl donor. Comparing with acyl donors and reaction temperature, the conversion yield reached about 18, 65 and $80\%$ with methacrylic acid, methyl methacrylate and vinyl methacrylate as acyl donor, respectively. And optimum reaction temperature was 60, 50, and 50^{circ}C, respectively