• 한국환경과학회:학술대회논문집
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• 한국환경과학회 2003년도 International Symposium on Clean Environment
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• pp.103-108
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• 2003

A method to determine chromium(III) ion in aqueous solution by chemiluminescence method using a lucigenin entrapped silica sol-gel film has been studied. An optical probe for chromium(III) ion has been prepared by entrapping lucigenin into silica sol-gel film coated on a glass support by dip coating. The chromium(III) optical sensor is based on the catalytic effect of chromium(IIII) ion on the reaction between lucigenin and hydrogen peroxide in basic solutions. The effects of Nafion, DMF and Triton X-100 were investigated to find the optimum condition to minimize cracking and leaching from the probe. The effects of pH and concentrations of lucigenin and hydrogen peroxide on the chemiluminescence intensity were investigated. The chemiluminescence intensity was increased linearly with increasing chromium(III) concentration from $2.5{\times}10^{-4}$M to $8.0{\times}10^{-7}$M and the detection limit was $4.0{\times}10^{-7}$M.

• 한국연소학회지
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• 제20권1호
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• pp.24-31
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• 2015

Flame structure, which contains a useful information for studying combustion instability of the flame, is often quantitatively visualized with PLIF (planar laser-induced fluorescence) and/or chemiluminescence images. The latter, a line-integral of a flame property, needs to be preprocessed before being inverted, mainly due to its inherent noise and the axisymmetry assumption of the inversion. A preprocessing scheme utilizing multi-division of ROI (region of interest) of the chemiluminescence image is proposed. Its feasibility has been tested with OH PLIF and $OH^*$ chemiluminescence images of SNG (synthetic natural gas) swirl-stabilized flames taken from a model gas turbine combustor. It turns out that the multi-division technique outperforms two conventional ones: those are, one without preprocessing and the other with uni-division preprocessing, reconstructing the SNG flame structure much better than its two counterparts, when compared with the corresponding OH PLIF images. It is also found that the Canny edge detection algorithm used for detecting edges in the multi-division method works better than the Sobel algorithm does.

• Bulletin of the Korean Chemical Society
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• 제30권1호
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• pp.102-106
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• 2009

A rapid and sensitive chemiluminescence (CL) method using flow-injection (FI) has been developed for the determination of mandelic acid which is based on the enhancement of mandelic acid to the CL intensity of ${Ru(bipy)_{3}}^{2+}$-Ce(IV) system. The enhancement effect was dependent on the concentration of mandelic acid, based on which, CL system was established for the determination of mandelic acid. The concentrations of ${Ru(bipy)_{3}}^{2+}$+, Ce(IV), and $H_2SO_4$ were optimized. Under the optimum experimental conditions, the linear range and detection limit are 1.46-342.0 ${\mu}g/ml$ and 0.072 ${\mu}g/ml$, respectively. The correlation coefficient (R) was 0.99732. The utility of this method was demonstrated by determining mandelic acid in capsules and human urine sample.

• Bulletin of the Korean Chemical Society
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• 제32권7호
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• pp.2187-2192
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• 2011

Hydrogen peroxide plays a key role as a second messenger in the normal cellular signaling but its overproduction has been implicated in various life-threatening diseases. Peroxalate chemiluminescence is the light emission from a three component reaction between peroxalate, hydrogen peroxide and fluorophores. It has proven great potential as a methodology to detect hydrogen peroxide in physiological environments because of its excellent sensitivity and specificity to hydrogen peroxide. We developed chemiluminescent micelles composed of amphiphilic polymers, peroxalate and fluorescent dyes to detect hydrogen peroxide at physiological concentrations. In this work, we studied the relationship between the chemiluminescence reactivity and stability of peroxalate by varying the substitutes on the aryl rings of peroxalate. Alkyl substitutes on the aryl ring of peroxalate increased the stability against water hydrolysis, but diminished the reactivity to hydrogen peroxide. Chemiluminescent micelles encapsulating diphenyl peroxalate showed significantly higher chemiluminescence intensity than the counterpart encapsulating dimethylphenyl or dipropylphenyl peroxalate. Diphenyl peroxalate-encapsulated micelles could detect hydrogen peroxide generated from macrophage cells stimulated by lipopolysaccharide (LPS) and image hydrogen peroxide generated during LPS-induced inflammatory responses in a mouse.

• Journal of Photoscience
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• 제12권3호
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• pp.137-141
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• 2005

The chemiluminescence reaction of lophine with $H_2O_2$ in alkaline solution has been investigated for use in determination of $Cu^{2+}$ ions. The observed chemiluminescence intensity is found to be a function of the concentration of $Cu^{2+}$. Under the optimum reagent concentrations such as $4{\times}10^{-4}M$ lophine, 0.8 M KOH, 0.2M $H_2O_2,{\lambda}_{em}$, 533nm, the linear range and the detection limit were found to be 0.048ug/ml-48.32ug/ml (R=0.99897) and 0.005ulg/ml respectively. Relative standard deviation for five determinations of 24.16ug/ml $Cu^{2+}$ is 2.35%. The interference from other species was investigated. The proposed method was applied to the determination of $Cu^{2+}$ in different water samples.

• Journal of Mechanical Science and Technology
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• 제18권10호
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• pp.1859-1868
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• 2004

Monitoring of OH chemiluminescence through an optical fiber was demonstrated to be a useful method in detecting self-excited combustion oscillations. OH chemiluminescence intensity detected by the optical fiber showed mostly excellent agreement with those obtained by high speed CCD camera measurements when combustion oscillations were strong. Symptoms of self-excited combustion oscillation were also studied in order to predict the onset of combustion oscillation before it proceeded to a catastrophic failure. For the purpose, we have found and proposed unique measures to tell the onset of self-excited combustion oscillations based on the careful statistics of fluctuating properties in flames, such as pressure or emission of OH radicals.

• 대한화학회지
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• 제45권3호
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• pp.223-229
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• 2001

흐름주입 장치를 이용하여 화학발광법에 의한 전혈 중의 당을 정량하는 방법에 대하여 연구하였다. 당의 효소반응에서 생성되는 과산화수소에 의하여 424nm에서 발생하는 luminol의 화학발광 세기의 차이를 정량에 이용하였다. 효소반응기는 glucose oxidase를 aminopropyl glass bead에 혼입하여 만들었으며, 흐름셀에서 발생하는 화학발광의 세기는 광섬유 다발을 이용하여 측정하였다. 최적 실험조건을 구하기 위하여 화학발광 시약 및 효소반응기의 pH, 흐름속도 및 온도가 화학발광세기에 미치는 영향을 조사하였다. 최적실험조건에서 구한 검정 곡선은 $1.0{\times}10^{-1}$~ 7.0mM에서 직선성이 성립하였으며, 검출한계는 $6.0{\times}10^{-2}$mM이었다. 본 방법을 전혈 중의 당 정량에 적용하였으며, 그 결과를 기존의 분석법에서 구한 결과와 비교하였다. 또한, 회수율 측정을 통하여 본 방법의 신뢰성을 검증하였다.

• 대한화학회지
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• 제46권2호
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• pp.145-150
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• 2002

자동흐름분석법으로 lucigenin을 발광시약으로 이용하여 화학발광의 세기를 측정함으로써 수용액 중의 Cr(III)을 정량하는 방법에 대하여 연구하였다. pH, 시료의 주입 양과 속도, lucigenin의 농도 및 방출파장이 방출세기에 미치는 영향을 조사하였다. Lucigenin과 과산화수소의 화학발광 반응에서 Cr(III)를 첨가하였을 때 방출세기가 현저히 증가함을 관찰하였다. Cr(III) 이온 검정곡선의 직선감응범위와 검출한계는 들뜸 파장, pH 및 lucigenin과 과산화수소의 농도가 각각 473 nm, 12.8 및 1.0${\times}10^{-6}$M과 2.0M였을 때, 1.0${\times}10^{-6}$M∼1.0${\times}10^{-3}$M 및 5.2${\times}10^{-8}$M이었다.

• Bulletin of the Korean Chemical Society
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• 제25권8호
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• pp.1177-1181
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• 2004

The determination of uric acid in urine samples was studied by a chemiluminescence measurement using tris (2,2'-bipyridine)ruthenium(II)-octylphenylpolyglycolether [Ru$(bpy)_3^{2+}$ -OPE] system. The oxidized uric acid by Ce(IV) excited Ru$(bpy)_3^{2+}$ to emit a chemiluminescence in this system so that the intensity was stoichio-metrically dependent upon the concentration of uric acid. In a reaction cell, a luminescent reagent, oxidant, surfactant and sulfuric acid were flowed into and mixed with a taken sample. Experimental conditions were optimized to obtain the maximum intensity of chemiluminescence. Each reactant solution of more than the following concentration gave a good result: $2\;{\times}\;10^{?4}$ M Ru$(bpy)_3^{2+}$ , 0.01 M Ce(IV), 6% OPE, and 0.33 M $H_2SO_4$. Any interferences were not shown in this process by the investigation of concomitant constitutes such as albumin, creatine, lactic acid, glucose, urea, $Cl^?,\; Mg^{2+},\;Ca^{2+}$ and so on. The linearity of a calibration curve was good with r = 0.998, the relative standard deviation of the slope was 3.3% and the detection limit was 5.6ng/mL. The recoveries of 80 to 91% were obtained from the standard spiked samples. The values were little bit low, but this procedure could be considered to be reliable for the determination of trace uric acid in urine samples.

• Journal of Photoscience
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• 제9권2호
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• pp.412-414
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• 2002

Several peroxovanadium(V) complexes with an organic chelate ligand decompose spontaneously, depending on the nature of the chelate ligand. The self-decomposition reactions of the dinuclear peroxovanadium(V) complex with 2-oxo-l,3-diaminopropane-N,N,N',N'-tetraacetate (dpot) and the peroxovanadium(V) complexes with N-carboxymethylhistidinate (cmhist) and histamine-N,N-diacetate (histada) accompany the reduction of vanadium(V) to vanadium(IV). This implies that the peroxide anion acts as a reducing agent and thus the peroxide is oxidized in the decomposition process of the peroxovanadium(V) complexes. The oxidized dioxygen species have been characterized spectrophotometrically. Superoxide anion has been detected in 2-3 % yields using the reduction of cytochrome c method and chemiluminescence method utilized MCLA as a fluorescer. Singlet oxygen has also been detected in higher yields on the basis of chemiluminescence of tryptophan.