• 한국패류학회지
• /
• 제20권1호
• /
• pp.93-105
• /
• 2004

Arkshell clams, Scapharca broughtonii, are economically important edible bivalves and widely cultivated in the Southern coast of Korea. Recently, the production of S. broughtonii has been dramatically decreased and various reasons including chemical pollution were suspected to be related to the production declines. However, it remains unknown whether the chemical pollution levels of the surrounding environments were high enough for the biological and ecological disturbance for the population of S. broughtonii, because no systematic toxicological study using S. broughtonii has been conducted previously. In the present study, we exposed arkshell clams, S. broughtonii to various waterborne pollutants including heavy metals (Cd, Cu and Hg), ammonia and organotins (tributyltin and triphenyltin) to determine the effect concentrations of these pollutants for the survival of S. broughtonii for 20 days. The median lethal concentrations ($LC_50$) of S. broughtonii were 2.1 mg/l for Cd, 0.065 mg/l for Cu, 0.40 mg/l for Hg, 79.4 mg/l for total ammonia (1.9 mg/l for unionized ammonia), 0.5 ${\mu}$g/l for TBT, and 14${\mu}$g/l for TPhT. Lethal toxicity of the most pollutants increased with both exposure duration and concentration. The toxicity of TBT was greatest for S. broughtonii, followed by TPhT > Cu > Hg > Cd > ammonia. The sensitivities of S. broughtonii to heavy metals and TBT were comparable to those of other aquatic organisms, but they were relatively tolerable to ammonia. The environmental concentrations of the tested pollutants were compared with the effect concentrations of those for the survival of S. broughtonii to assess the potential risks of the pollutants in the field conditions.

• Journal of Microbiology and Biotechnology
• /
• 제4권2호
• /
• pp.119-125
• /
• 1994

Steroid $\Delta^1$-dehydrogenase purified from hydrocortisone-induced cells of Arthrobacter simplex converted various 3-ketosteroids into their corresponding $\Delta^1$-dehydrogenated products. The transformation efficiencies depend upon the chemical structure of the steroids, especially length of the side chain at 17 position and hydroxyl groups at 11 and 17 positions. The Km values for androstenedione, the most favorable substrate examined, and hydrocortisone were 74 ${\mu}M$ and 294 ${\mu}M$, respectively. The optimum temperature and pH of the enzyme reaction were 35$^{\circ}C$ and pH 9, respectively, and the enzyme was relatively stable at the range from 20 to 35$^{\circ}C$ and from pH 5 to 10 after one hour of incubation. The enzyme activity was markedly inhibited in the presence of $Cu^{2+},\;Fe^{3+},\;Hg^{2+},\;Mo^{6+}$ ions, and somewhat inhibited by $Zn^{2+}$ and $Fe^{2+}$. $\alpha,\alpha'$-Dipyridyl that inhibits 9$\alpha$-hydroxylase and accumulates 1,4-androstadiene-3,17-dione from sterols revealed no inhibitory effect on this enzyme. EGTA showed inhibitory effect. $\beta$-Estradiol competitively inhibited the enzyme activity. Chemical modifications of the enzyme were attempted with several reagents. p-Hydroxymer-curibenzoate showed inhibition of the enzyme activity and protection of the substrate. This suggests that cysteine residue may be involved in the active site of the enzyme.

• Archives of Pharmacal Research
• /
• 제23권2호
• /
• pp.128-138
• /
• 2000

In continuation of the previous work (Fathalla, 1992) on the synthesis of some heterocycles containing uracil moiety, we report herein the incorporation of uracil moiety into cyan-opyridine thione, thiosemecarbazone, semicarbazone, cyanopyridine, ami nocyano pyridine, isoxazoline, pyrazoline, pyrimidine, triazolo pyrimidine, pyran, selena and thiazole derivatives which might modify their biological activities. The biological studies revealed that the chemical compound III f showed high molluscicdal activity than other compounds. The profile of the nucleoprotein extracted from chemically (compound IIIc, e, f and g) treated or UV-irradiated B.alexandrina snails did not show appreciable differences when compared to non-treated (native) snails by using SDS-PAGE, where no obvious qualitative or quantitative differences were observed. Immunization of experimental animals with the nucleoprotein extracted from native, chemically (compound III f ＆ g) treated or physically treated B.alexandrina snails induced significant protection against challenge with normal S.mansoni cercariae, as compared to the non-immunized challenged control. As well as , a decrease in the number of granuloma formation and the size range of granuloma was also observed in immunized animals. It is concluded that, compounds III f and g have a potent molluscicidal activity. They also induced chemical modification comparable to that induced by physical treatment in the snail's nucleoprotein, which could possibly be used in immunization against S. mansoni infection.

• Korean Chemical Engineering Research
• /
• 제50권1호
• /
• pp.112-117
• /
• 2012

나노기술의 발전과 함께 나노물질을 포함한 소비재가 대중화되고 있다. 그러나 지난 10여년간 조심스럽게 제기되고 있는 나노물질의 잠재적 위해성으로 인해, 나노제품 사용을 불안해하고 있다. 특히 나노제품을 직접 취급하는 생산시설(연구소 및 업체)의 작업자는 직접적인 인체 노출을 초래하게 된다. 따라서 이들에 대한 인체 및 환경 노출 안전관리를 위하여, 직접적인 노출평가가 필요하다. 본 연구에서는 기상 및 액상 반응을 통해 나노물질을 생산하는 두 곳의 업체를 현장 방문하여 나노물질의 주요 노출대상 공정과 노출원을 파악하고 SMPS를 이용한 실시간 현장 모니터링을 실시하였다. 분석 결과, 액상 공정도 기상으로의 나노입자 노출이 심각하게 발생하고 있음을 확인하였다. 가장 문제가 되는 점은 나노물질의 잠재적인 위해성에 관한 인식의 부족으로 제대로 된 방호활동을 못하고 있다는 점이다. 따라서 보다 다양한 나노물질 취급 시설에 대한 환경노출 평가가 필요하고 이를 바탕으로 한 나노물질 취급 안전관리 방법이 제시되어야 한다.

• Bulletin of the Korean Chemical Society
• /
• 제28권10호
• /
• pp.1729-1734
• /
• 2007

The electrochemical behaviors of estrone in the presence of various surfactants were examined with great details. It is found that a cationic surfactant, cetyltrimethylammonium bromide (CTAB), obviously facilitates the electro-oxidation of estrone at carbon paste electrode (CPE) from the significant peak current enhancement and the negative shift of peak potential. Additionally, chronocoulometry and electrochemical impedance spectroscopy (EIS) were also used for further investigation of the electrode process of estrone, indicating that low concentration of CTAB exhibits excellent enhancement effects on the electrochemical oxidation of estrone, greatly enhances the diffusion coefficient and the electron transfer rate. Based on this, an electrochemical method was proposed for the determination of estrone. The oxidation peak current is proportional to the concentration of estrone in the ranges over 9.0 × 10?8 - 8.0 × 10?6 mol/L, and a low detection limit of 4.0 × 10?8 mol/L was obtained for 180s accumulation at open circuit (S/N = 3). Finally, this proposed method was demonstrated using estrone tablets with good satisfaction.

• 한국시뮬레이션학회논문지
• /
• 제30권2호
• /
• pp.49-64
• /
• 2021

화학·생물(화생) 위험을 초기 단계에 효과적으로 대응하기 위해서는 화생 대응 계획을 체계적으로 발전시켜야 하며, 모델링 및 시뮬레이션은 이를 위한 과학적 수단으로 활용될 수 있다. 그러나 오염 확산 모델링 분야는 많은 발전을 이루고 있으나, 화생 대응 계획을 모의하고 적절성을 분석하는 시뮬레이션 분야는 여전히 초기 단계에 머무르고 있다. 이에 본 논문에서는 화생 오염 탐지, 보호, 제독 등 대응 계획을 과학적으로 모의하기 위한 모델을 제안한다. 먼저 기상 및 지형 조건을 고려하여 예측된 오염 확산 결과를 교전 모델에 반영하는 방법을 제시한다. 이어서 공개된 사상자 예측 기법을 기반으로 전투 모의 개체의 화생 피해를 모의하는 화생 전투 피해 모의 기법을 설계한다. 그리고 화생 위험 탐지·정찰, 제독, 보호 등 화생 위험 대응 계획을 체계적으로 모의하는 과업을 모델링한다. 끝으로 화생 감시소 운용에 의한 오염 탐지의 신속성을 분석하는 한편, 화생 제독소 운용 시 오염 부대 규모와 제독 부대 규모에 따른 제독 소요 시간을 분석함으로써 화생 전투 모의 실험의 가능성을 확인한다. 제안된 모델을 이용하면 향후 군의 화생 방호 체계 및 운용개념에 대한 효과 분석은 물론 재난 방재 및 모의 훈련 분야에서도 일부 활용이 가능할 것으로 기대된다.

• Journal of Ginseng Research
• /
• 제38권3호
• /
• pp.215-219
• /
• 2014

Background: Biological control of plant pathogens using benign or beneficial microorganisms as antagonistic agents is currently considered to be an important component of integrated pest management in agricultural crops. In this study, we evaluated the potential of Bacillus subtilis strain HK-CSM-1 as a biological control agent against Colletotrichum panacicola. Methods: The potential of B. subtilis HK-CSM-1 as a biological control agent for ginseng anthracnose was assessed. C. panacicola was inoculated to ginseng plants and the incidence and severity of disease was assessed to examine the efficacy of the bacterium as a biological control against C. panacicola. Results: Inoculation of Panax ginseng plants with B. subtilis significantly suppressed the number of disease lesions of C. panacicola and was as effective as the chemical fungicide iminoctadine tris(albesilate). The antifungal activity of B. subtilis against C. panacicola was observed on a co-culture medium. Interestingly, treatment with B. subtilis did not significantly affect the diameter of the lesions, suggesting that the mechanism of protection was through the reduction in the incidence of infection related to the initial events of the infection cycle, including penetration and infection via spore germination and appressorium formation rather than by the inhibition of invasive growth after infection. Conclusion: Our results suggest that B. subtilis HK-CSM-1 can be used as an effective and ecologically friendly biological control agent for anthracnose in P. ginseng.

• 한국환경보건학회지
• /
• 제31권5호
• /
• pp.365-371
• /
• 2005

This study was peformed to explore the current situation of university environmental health and safety (EHS) system and evaluate the effects of such system on university EHS practices. Essential elements for the university EHS system were identified based on the representative examples of foreign universities, and Hey were incorporated into a questionnaire, which was used in this study. Among the academic institutions we surveyed in this study, 89% of the universities employed health and safety manager, and 65% kept departments. However, less than 50% of universities maintained EHS policies, maintained health and established safety committees within the university headquarters, and held health and safety meetings on regular basis. Several basic requirements such as chemical hygiene program and laboratory safety training program lacked in many universities. Some basic surveillance was performed in many universities, however, only few universities carried out surveillance on biological safety, medical examination, and chemical exposure monitoring. EHS system had significant influence on university EHS program (p<0.05). Performances of EHS activities were influenced by presence of EHS policies, and university health and safety committees.

• 한국식품영양과학회지
• /
• 제23권5호
• /
• pp.867-878
• /
• 1994

A general overview of the lipid peroxidation and its nutritional significance are presented ,with emphasis on the reaction mechaisms, peroxidized products, further interaction and nutritional/biological deterioration in a series of oxidative process. Overall mechanism with various factors and elements for initiation , propagation and termination of free radical reaction is reviewed and the primary /secondary products of peroxidized lipids are defined. Since these products are potentially reactive substances that can cause deterioration of proteins /amino acids and vitamins (carotene, tocopherols and ascorbic acid etc), mechanism and actual damages of their deterioration in some foods and biological models are outlined. Especially , chemical changes caused by interaction of peroxidized products (related hydroperoxides, radicals and malonaldehye etc) and protein are emphasized here. And also, the detailed mechanisms on radical scavenging of the these vitamins which are the most prominent natural antioxidants are presented . Additionally , the possible roles of peroxidicaed lipids and their secondary products in the process of aging an carcinogenesis are briefly discussed . However, it is important to not that more detailed and integrated studies on the reaction kinetics, energetics of peroxidation, their decomposed products , biochemical interaction potential damaging/aging / carcinogenic effects, protection from their oxidative spoilage and novel antioxidants in food and heterogeneous biological systems will be essential in order to assessing the implication of lipid peroxidation to human nutrition and health.

• 한국산학기술학회논문지
• /
• 제20권5호
• /
• pp.87-92
• /
• 2019

본 논문은 집단보호장비에 들어가는 회로카드조립체의 고장 원인 분석 및 품질 개선에 관한 연구이다. 해당 장비는 현재 운용중인 무기체계의 구성품으로 냉난방 기능뿐만 아니라, 화생 방어 역할을 한다. 그런데 군에서 운용중에 응축부조립체의 팬이 동작하지 않는 현상이 다수 발생되었다. 이에 따라 고장 원인을 분석하였고 특정 회로카드조립체가 소손됨을 확인하였다. 고온의 환경조건에서 지속적인 냉방가동에 따라 부품이 가열되고 이에 따라 고온에 노출된 전자부품이 열화되어 소손됨을 알 수 있었다. 따라서 본 논문은 이를 해결하기 위해 방열판을 적용하여 과온 동작에 의한 고장빈도를 낮추고 회로카드조립체의 수명을 연장한 품질 개선에 관한 연구이다. 개선된 회로카드조립체는 실험을 통해 방열성능을 확인하였다. 뿐만 아니라 체계 호환성 검사, 양압유지, 소음 시험, 작동시험 등을 통해 성능검사를 마쳤으며 현재 개선된 제품을 적용중이다. 이번 개선을 통해 현재까지 해당 회로카드조립체에서 발생한 고장은 없으며 해당 장비의 품질이 향상됨을 확인하였다.