• Applied Biological Chemistry
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• 제37권4호
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• pp.310-314
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• 1994

솔잎으로부터 프리 라디칼 소거 작용이 있는 물질을 분리하고, 여러 기기 분석 결과에 근거하여 4-hydroxy-5-methyl-3[2H]-furanone으로 동정하였다. 아울러 xylose와 glycine의 Maillard 반응을 통하여 이 물질을 합성하였다. 이 물질은 1,1-diphenyl-2-picrylhydrazyl(DPPH) 프리 라디칼에 대해 소거 작용을 보였으며(100 M의 DPPH의 50%를 환원 시키는데 필요한 농도, 즉 $SC_{50}=26\;{\mu}M$) 이는 구조적으로 유사한 공지의 항산화 물질인 $4-hydroxy-2,5-dimethyl-3[2H]-furanone(SC_{50}=53\;{\mu}M)$과 $3-hydroxy-2-methyl-{\gamma}-pyrone(SC_{50}=4.0\;mM)$에 비해 강력한 것이다. 4-Hydroxy-5-methyl-3[2H]-furanone은 3,4-dihydroxyphenylalanine과 linolenic acid의 자동 산화에 대해서 억제 작용을 나타내었다.

• Journal of Microbiology and Biotechnology
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• 제16권11호
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• pp.1819-1825
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• 2006

Four naphthalene-degrading bacteria (Pseudomonas sp. strains O1, W1, As1, and G1) were isolated feom pollutant-contaminated sites. Examination of their substrate utilization and analyses of key naphthalene-catabolic regulatory genes revealed that the pathway and regulation of naphthalene-degradation in all four strains resemble those of NAH7 from P. putida G7. Superoxide anion production, superoxide dismutase activity, and catalase activity during their growth on naphthalene-amended medium increased significantly, compared with those with glucose-amended medium. Addition of ascorbate, an antioxidant, or ferrous iron ($Fe^{2+}$) increased the growth rates of all tested microorganisms on naphthalene. Northern blot and HPLC analyses showed that both nahA gene expression and naphthalene degradation increased under those conditions. Our data suggest that naphthalene degradation can impose severe oxidative stress, and defenses against oxidative stress would play an important role in the metabolism of naphthalene.

• Parasites, Hosts and Diseases
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• 제53권6호
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• pp.683-688
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• 2015

Human diphyllobothriasis is a widespread fish-borne zoonosis caused by the infection with broad tapeworms belonging to the genus Diphyllobothrium. In mainland China, so far 20 human cases of Diphyllobothrium infections have been reported, and the etiologic species were identified as D. latum and D. nihonkaiense based on morphological characteristics or molecular analysis. In the present study, proglottids of diphyllobothriid tapeworms from 3 human cases that occurred in Heilongjiang Province, China were identified as D. nihonkaiense by sequencing mitochondrial cytochrome c oxidase subunit I (cox1) and NADH dehydrogenase subunit 5 (nad5) genes. Two different cox1 gene sequences were obtained. One sequence showed 100% homology with those from humans in Japan. The remaining cox1 gene sequence and 2 different nad5 gene sequences obtained were not described previously, and might reflect endemic genetic characterizations. D. nihonkaiense might also be a major causative species of human diphyllobothriasis in China. Meanwhile, the finding of the first pediatric case of D. nihonkaiense infection in China suggests that infants infected with D. nihonkaiense should not be ignored.

• 천문학회보
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• 제43권1호
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• pp.55.1-55.1
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• 2018

Studies on rotation-powered pulsars with strong surface magnetic field may help us clarify the unclear link between magnetars and canonical radio pulsars because the magnetar-like emission is expected to be observed. PSR J1119-6127 associated with SNR G292.2-0.5 has a high magnetic field of $4.1{\times}1013$ gauss, and a young characteristic age of ~1700 years can be served as the good candidate to compare with magnetars and rotation-powered pulsars. The glitch accompanied by the radiative changes detected in 2007 is the first case we observed for a rotationally powered radio pulsar. This pulsar experienced magnetar-like outbursts in mid. 2016, similar to the 2006 transition occurred on the other radio-quiet rotation-powered pulsar with strong surface magnetic field, PSR J1846-0258. In this talk, I'll report the investigation with X-ray and gamma-ray data of this magnetar-like pulsar. A sudden decrease in the gamma-ray emission at the GeV band was detected immediately after the X-ray outburst. Accompanying with the disappearance of the radio pulsation, the gamma-ray pulsation cannot be resolved as well after the outburst. We tried to derive the timing behavior and some intriguing features of this pulsar in this work corresponding to the outburst using the Swift data, NuSTAR and XMM observations.

• 보건의료산업학회지
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• 제7권1호
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• pp.155-165
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• 2013

This study aims to identify the significance of adolescent suicidal ideation in a multicultural family, propose suicidal prevention strategies, and provide considerable foundations supporting their secured future. The research results are in the follow. For the results of main factors of relative influence on the suicidal ideation, the relative dangerousness levels were 5.5 times more likely to experience with drugs, 4.5 times more likely to have depressive symptoms, 3.2 times more likely to experience violence at school, and 2.8 times more likely to experience drinking alcohol, 1.4 times more likely to feel unhappiness and 1.3 times more likely to be bad off in economic status, all of which contribute to suicidal ideation. All results turned out to be statistically significant. Therefore, the findings and results of this study indicate that appropriate and practical prevention strategies to address the suicidal behavior of adolescents in a multicultural family need to be carefully considered and designed in a most urgent manner.

• 한국식품영양학회지
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• 제27권4호
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• pp.609-618
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• 2014

본 실험에서는 조리과정(non-blanched, blanched, seasoned) 중 시래기의 항산화 관련 물질 함량과 항산화 활성 및 항균활성의 변화를 측정하여 한식 고유의 조리법인 나물의 우수성을 알리고자 하였다. 80% ethanol에 추출한 시래기의 수율은 무친 후가 7.55%로 가장 높았으며, 데치기 전이 4.91%를 나타내었고, 데친 후가 0.33%로 가장 낮은 수율을 보였다. 총 폴리페놀 함량은 3.57~129.85 mg GAE/100 g FW 범위로 무친 후 시래기가 $129.85{\pm}0.62mg$ GAE/100 g FW로 가장 높게 나타났으며, 총 플라보노이드 함량에서도 무친 후가 $35.56{\pm}1.19mg$ CHE/100 g FW로 가장 높은 함량을 나타냈다. 4가지의 항산화 실험(DPPH assay, ABTS assay, FRAP assay, Reducing power) 결과, 무친 후 > 데치기 전 > 데친 후 순으로 높은 활성을 보였다. 항균 활성 측정 결과, 데치기 전 시래기에서만 활성이 나타났는데, B. cereus와 E. cloacae 두 균에 대해서 농도 의존적으로 항균 활성을 나타냈고, E. coli와 P. aeruginosa 두 균에 대해서는 10 mg/disc의 농도에서만 각각 9.25 mm, 9 mm의 clear zone을 생성하였다. 상관관계 분석 결과, 총 폴리페놀과 총 플라보노이드 함량이 증가할수록 radical 소거 활성 및 환원력의 활성이 증가하여 시래기에 함유된 총 폴리페놀 및 플라보노이드 함량이 항산화 활성에 중요한 영향을 미치는 것으로 사료된다. 따라서 항균 활성 측면에서는 시래기를 조리하지 않고 섭취하는 것이 좋으나, 건조 나물을 섭취하기 위해서는 특성상 반드시 불린 후 조리하는 작업이 필요하다. 또한 무치거나 조리하는 과정에서 갖은 양념이 첨가되어 항산화 효능이 증가하므로 시래기를 섭취할 경우, 가능하면 장시간 물에 삶는 조리법은 피하고, 무치는 조리법을 사용하는 것이 건강에 이로울 것으로 사료된다.

• 미생물학회지
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• 제46권4호
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• pp.359-365
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• 2010

조릿대 근권토양으로부터 분리한 방선균 50균주를 대상으로 고추 세균성 점무늬병원균(Xanthomonas axonopodis pv. vesicatoria)의 항균활성 12균주를 선발하였다. 이들 항균활성 12균주의 계통학적 위치를 검토한 결과, 모두 Streptomyces 속의 Cluster II에 속하는 특징을 나타내었다. JR-24 균주는 최소저해 농도(MIC) 10 ${\mu}l$/disc를 나타내었으며, 배양액 5 ${\mu}l$/ml를 처리 하여 12시간 배양한 결과 Xanthomonas axonopodis pv. vesicatoria에 강한 생육저해효과를 나타내어 최우수 균주로 선발되었다. 항균활성 균주 JR-24의 16S rRNA 유전자 염기서열을 검토한 결과, Streptomyces galbus $DSM40089^T$ (X79852)와 98.1%, Streptomyces longwoodensis $LMG20096^T$ (AJ781356)와 98% 그리고 Streptomyces capoamus $JCM4734^T$ (AB045877)와 97.8%의 상동성을 나타내었다. API 20NE와 API 50CHE를 이용하여 JR-24 균주의 생리 생화학적 특성을 확인한 결과, L-arabinose, D-fructose, D-glucose, D-galactose을 이용하며 gelatin, protein, starch에 대하여 분해능이 있는 것으로 확인되었다. 주요지방산으로는 iso-$C_{14:0}$ (25.93%), iso-$C_{15:0}$ (10.13%), anteiso-$C_{15:0}$ (19.29%) 그리고 iso-$C_{16:0}$ (20.35%) 등을 함유하였으며, 퀴논종은 MK-9 ($H_4$) 4.37%, MK-9 ($H_6$) 51.22% 그리고 MK-9 ($H_8$) 49.47%로 동정되었다. Streptomyces sp. JR-24 균주의 계통학적 특성을 근연종인 Streptomyces galbus $DSM40089^T$와 비교한 결과, 다수의 표현형적 및 계통학적 차이를 나타내었다. 본 연구에서 분리된 Streptomyces sp. JR-24는 친환경 미생물제제 개발을 위한 유전자원 확보에 있어서 매우 큰의의가 있을 것으로 사료 된다.

• 한국실내디자인학회논문집
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• 제21권4호
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• pp.190-199
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• 2012

In this era of information, the public library takes a important role in the local community. In several years, public libraries have been proliferated in our society. However, there have not been specific planning standards of space size for construction of public library. The situation has lead the local governments to the need of consulting for the construction and management of library in search of appropriate guideline. The research paper focuses on the result of consulting support project for construction and management of public library. It examines total 11 libraries which were consulted from 2009 to 2011. It examines the configurational differences between the first scheme from the local government and the final scheme after the consulting, and the percentages of gross areas of each parts of library, reference and reading area, office area, education and cultural area, and public common use area. The analysis concludes that reference and reading area increased by 6.4% from 34.1% to 40.5%, and public common use area reduced by 6.1% from 30.2% to 24.1%, which represents a certain tendency of the configuration ratio of the each parts. Thus, the paper could be a starting point of establishing planning standard of space size for public library.

• 한국운동역학회지
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• 제27권3호
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• pp.181-187
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• 2017

Objective: The aim of the study was to compare & analyze on the variations of ground reaction force during ascending and descending of bus stair. Method: Simulated wooden stair of bus (raiser: 37.66 cm, width: 109 cm, tread: 29 cm) and GRF system (AMTI-OR-7/ AMTI., USA) was set up within experimental room. Adult female (n=8) performed ascending & descending of simulated bus stair, and variables analyzed consisted of TT (transfer-time), PVF (peak vertical force), LR (loading rate), DR (decay rate), CV (coefficient of variation) and AI (asymmetry index). Sample data from GRF cut off at 1,000 Hz. Results: TT showed shortest variation at phase 1 during descending, but longest variation at phase 1 during ascending of stair. PVF19 (Fz2, 100%) showed large pattern during descending than that of ascending, but rather showed small pattern during ascending of stair in case of PVF2 (Fz4). LR showed larger pattern during descending than that of ascending, but rather during ascending of stair in case of DR. Variation of CV (%) did not show difference between LR and DR, but showed higher possible occurrence of variation during descending of stair. Also AI (%) showed higher index during ascending than that of descending of stair. Conclusion: Because introduction of lowered bus stair has various realistic problems, if lined up at designated bus stopage exactly, rather can solve problems of inconvenience, reduce impulsive force and secure a stability of COG during ascending & descending of stair.

• 열처리공학회지
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• 제9권2호
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• pp.79-90
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• 1996

Transmission electron microscopy(TEM) investigation on the phase decomposition of B2-ordered (Ni,Co)Al supersaturated with Ni and Co has revealed the precipitation of $(Ni,Co)_2Al$ which has not been expected from the reported equilibrium phase diagram. The $(Ni,Co)_2Al$ phase has a hexagonal struture and takes a rod-like shape with the long axis of the rod parallel to the <111> directions of the B2 matrix. By aging at temperatures below 873 K, a long period Superlattice Structure appears in the hexagonal $(Ni,Co)_2Al$ Phase. The orientation relationship between the $(Ni,Co)_2Al$ Precipitates and the B2-(Ni,Co)Al matrix is found to be$(0001)_p$ // $(111)_{B2}$ and $[\bar{1}2\bar{1}0]_P$ // $[\bar{1}10]_{B2}$, Where the suffix p and B2 denote the $(Ni,Co)_2Al$ precipitate and the B2-(Ni,Co)Al matrix, respectively. (Ni,Co)Al hardens appreciably by the fine precipitation of the $(Ni,Co)_2Al$ phase. Energy dispersive spectroscopy was used to analyze the compositions of each phase formed in B2-(Ni,Co)Al.