• Journal of Korean Society of Forest Science
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• v.103 no.3
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• pp.375-382
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• 2014

The objective of this study was to investigate suitable plantation site for planted Chamaecyparis obtusa seedlings from the analysis of regional early growth performances. Two years old C. obtusa seedlings were planted with the density of $3,900seedlings{\cdot}ha^{-1}$ in late March, 2011 at Haeman and Jangseong, Jeollanamdo. In each study site, three plots ($400m^2$ per plot) were established and root collar diameter (mm) and tree height (cm) of each C. obtusa were measured in April, 2011 and October from 2011 to 2013. We also analyzed soil physical and chemical properties of sites and compartmental nitrogen and phosphorus concentrations of C. obtusa. Although the concentrations of soil nitrogen, organic matter, and C.E.C. at Haenam site were higher than those at Jangseong site, early growth performances of planted C. obtusa at Jangseong site were significantly better than those at Haenam site. The reasons for these results were probably related to deep available soil depth at Jangseong site and relatively low annual precipitation and sea wind at Haenam site, which was adjacent to the sea. The compartmental nitrogen and phosphorus concentrations of C. obtusa was in the order of needles > current twigs > fine root > stem and branches root above 2 mm in diameter. The phosphorus concentration of needles at Haenam site was significantly higher than that at Jangseong site. The results of this study might be useful for the selection of suitable plantation site for C. obtusa.

• Journal of the Korean Applied Science and Technology
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• v.40 no.6
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• pp.1268-1277
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• 2023

In this study, to evaluate the possibility of utilizing Chamaecyparis obtusa (Siebold & Zucc.) Endl. (C. obtusa) leaf fractions as anti-inflammatory functional materials, C. obtusa extract extracted with 99% ethanol (CO99EL) was fractionated with hexane (CO99EL-H), chloroform (CO99EL-C), ethyl acetate (CO99EL-E), butanol (CO99EL-B) and distilled water (CO99EL-W). The anti-inflammatory effects of each fraction was performed using lipopolysaccharide (LPS)-induced RAW264.7 mouse macrophages. Cytotoxicity was highest in CO99EL-H and CO99EL-C and lowest in CO99EL-W. Interestingly, LPS-induced iNOS expression and NO production were significantly reduced by CO99EL-H and CO99EL-E, and COX-2 expression was significantly reduced by CO99EL-B and CO99EL-W. In addition, interleukin (IL)-1𝛽, an inflammatory cytokine increased by LPS, was significantly reduced by CO99EL-C, CO99EL-E, CO99EL-B and CO99EL-W, and IL-6 was significantly reduced by CO99EL-B and CO99EL-W. Therefore, the janus kinase (JAK)/signaling transducer and activator of transcription (STAT) signaling pathway activated by LPS was significantly reduced by CO99EL-H and CO99EL-C, and the mitogen-activated protein kinase (MAPK) signaling pathway was slightly reduced by CO99EL-H and CO99EL-C. However, nuclear factor (NF)-𝜅B activity was not reduced by any fractions. Based on the results of this study, it was confirmed that CO99EL fractions have different anti-inflammatory mechanisms depending on the solvent used for fractionation.

• Journal of the Korean Wood Science and Technology
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• v.36 no.6
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• pp.159-167
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• 2008

This study was carried out to investigate the potential promise of Chamaecyparis obtusa oil as a natural antioxidant. C. obtusa oil and its fractions were subjected to screening for their antioxidant activities by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and ammonium thiocyanate method. In the first case, $IC_{50}$ value of the C. obtusa oil was determined as $40{\mu}{\ell}/m{\ell}$. At $0.5{\mu}{\ell}/m{\ell}$ concentration level, free radical scavenging effect of fraction G determined as 66.94% was the highest among the fractions of C. obtusa oil. In the ammonium thiocyanate method, essential oil of C. obtusa and its fraction C, D, and E showed activities of 72.0%, 71.2%, 71.9% and 71.1%, respectively. Fraction G, most active fraction, was mainly consisted of $\alpha$-terpineol, elemol, widdrol and $\alpha$-cadinol.

• Journal of Veterinary Clinics
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• v.16 no.2
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• pp.253-256
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• 1999

The influence of essential oils from aboriginal softwoods as Pinus densiflora, Pinus koraiensis, Chamaecyparis obtusa and Chamaecyparis pisifera on the motor coordination, pulse, mean blood artery pressure and pain reducing test was investigated in mice and rats. The motor coordination of mouse was not induced by the inhalation of each oil. Furthermore, these oils did not alter the changes of the mean blood pressure and pulse rate. Chamaecyparis pisifera trand to reduce the mean blood pressure. The abdominal pain induced by acetic acid was reduced only by an essential oil from Pinus koraiensis in mouse.

• Journal of the Korean Wood Science and Technology
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• v.39 no.4
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• pp.291-302
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• 2011

The study was to investigate whitening and antioxidation effects by determining the tyrosinase inhibition activity, DPPH radical scavenging and superoxide dismutase like activity of essential oils from Cryptomeria japonica and Chamaecyparis obtusa. The aim of the present study was to suggest preliminary data for research whitening and anti-oxidant effects material of C. japonica and C. obtusa essential oils and confirm supplementary worth of natural volatile organic compounds (nVOCs). Essential oils of C. japonica and C. obtusa leaves were extracted by steam distillation method of clevenger type, and nVOCs were extracted by high-temperature reactor for utilizing nVOCs condensates released during drying of C. japonica and C. obtusa at 80, 100, and $120^{\circ}C$ temperature conditions, respectively. In the activity of whitening and antioxidation, C. japonica oil was more effective than C. obtusa oil. nVOCs of C. japonica and C. obtusa showed highly activity of tyrosinase inhibitory at higher temperature. Antioxidation activity only shown on nVOCs of C. japonica produced at $120^{\circ}C$.

• Journal of the Korean Wood Science and Technology
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• v.33 no.3 s.131
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• pp.72-78
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• 2005

The present study was conducted to evaluate the application of Chamaecyparis obtusa and to investigate potential utilization of essential oil from C. obtusa as plant-based medicine. The antifungal activity of essential oil from leaves and twigs of C. obtusa (Sieb. Et Zucc) was determined and the major components of active fractions against Microsporum canis (KCTC6591) and Trichophyton mentagrophytes (KCTC6077) were identified by GC/MS analysis. In treatment of essential oil from C. obtusa, the strain M. canis was more resistant than the other, T. mentagrophytes. In the agar diffusion assay, essential oil from C. obtusa inhibited hyphal growth of M. canis and T. mentagrophytes at the concentration of more than 5,000 ppm. The zones named B and C in the TLC assay of essential oil from C. obtusa showed antifungal activities. Among four sub-fractions of n-hexane extract from B and C zones, named as B-1, B-2, C-1 and C-2, the C-2 showed the highest antifungal activity. Instrumental GC/MS analysis for sub-fractions showed that a major component of C-1 was ${\alpha}$-terpineol as terpene alcohol, while C-2 contained sesquiterpenes such as elemol, cedrol and eudesmol.

• Journal of Forest and Environmental Science
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• v.30 no.3
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• pp.285-292
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• 2014

Essential oil yields and chemical compositions from 5 populations of Chamaecyparis obtusa with several environmental factors were investigated through essential oil extracted distillation apparatus and metabolite profiling by GC-MS analysis. Among the populations, content of essential oil at Gokseong was significantly higher than other populations. To compare the several environmental factors affecting on chemical composition and essential oil yields from C. obtuse at Gokseong, the environmental factors (soil condition, temperature, humidity, and moisture content) were measured during 1 year. The essential oils at Goksung based on humidity on March, July, and November was significantly different from other months. The essential oils at Goksung based on temperature on July and August was significantly different from other months. The essential oils at Goksung based on the moisture content on September were significantly different from other months. The percentage of T-N, OM, and yield of oil at Gokseong were significantly different on from other populations. The main constituents of C. obtusa at all populations were ${\alpha}$-pinene, ${\beta}$-pinene, ${\alpha}$-terpinene, ${\gamma}$-terpinene, terpinene-4-ol, isobonyl acetate, terpinyl acetate, and cedar acetate. Specially, Essential oil compositions (%) of ${\alpha}$-terpinene and cedar acetate were higher at Gokseong than at other populations. The chemical compositions of essential oils were variable depend on populations and environmental conditions. Therefore, this study might be used as fundamental research on study for selection of high productive terpenoids and for understanding about biosynthesis of essential oils in C. obtusa.

• Korean Journal of Agricultural and Forest Meteorology
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• v.14 no.4
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• pp.236-245
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• 2012

This research was performed to analyze growth performance and pattern of eighteen families of hinoki cypress (Chamaecyparis obtusa), which were imported in Jeju region. The growths of 'Yako-7', 'Muei-1', and 'Sangsong-9' families were superior to other families whereas those of 'Sanguen-3', 'Yago-6', and 'Hyena-2' families were poor. The height growth between age 9-year to 15-year was superior to those between other ages. No specific growth pattern was observed in the diameter at breast height (DBH) by ages. Family heritability of height, DBH, and volume were estimated at 0.68, 0.75, and 0.75, respectively. Pearson's correlation analysis showed that there was little juvenile-mature correlation in the growth of C. obtusa.

• Journal of the Korean Wood Science and Technology
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• v.30 no.1
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• pp.48-55
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• 2002

The essential oils were extracted from the needles of several softwood tree species, Pinus densiflora, Pinus koraiensis, Chamaecyparis obtusa and Chamaecyparis pisifera to investigate their biological activities. The antifungal activity by hyphal growth inhibition method was determined by three wood rot fungi, one mouldous fungi, and three tree pathogenic fungi and the antibacterial activity by paper disc method and turbidiometric method by three gram positive bacteria and one gram negative bacteria, Escherichia coli, were also tested. Antifungal activity was the best in the essential oil from C. obtusa, and the following was resulted in order of P. densiflora and P. koraiensis. The essential oil from P. densiflora showed higher antifungal activity against wood rot fungi than that from P. koraiensis while the result was contrary against tree pathogenic fungi. However, the antibacterial activity was the best in the essential oil from P. densiflora, and the following was in order of C. obtusa and P. koraiensis. From these results, the variation of biological activity seemed to be due to the chemical composition of essential oils.

• Journal of Convergence for Information Technology
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• v.12 no.5
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• pp.126-132
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• 2022

Wood Plastic Composite(WPC) has been mostly used for outdoor purposes such as deck materials and trails so far. In this study, WPC panels with improved antibacterial properties, total volatile organic compound emissions (TVOC), and flame retardant were manufactured to use Wood Plastic Compound as interior materials for indoor use. WPC compound was prepared by mixing Chamaecyparis obtusa wood flour with high density polyethylene(HDPE). The prepared WPC compound exhibited excellent antibacterial and antifungal properties, and the total volatile organic compound emission(TVOC) was 0.062 mg/m2·h. The WPC panel(303mm×606mm×10mm) manufactured by a twin screw extruder with the manufactured compound achieved the flame retardant grade 2 standard of KS F 2271.