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Investigation of Active Antifungal Compounds of Essential Oil from Chamaecyparis obtusa Against Dermatophytes, Microsporum canis and Trichophyton Mentagrophytes  

Park, Mi-Jin (Dept. of Forest Science, College of Agriculture & Life Science, Seoul national university)
Lee, Soo-Min (Dept. of Forest Science, College of Agriculture & Life Science, Seoul national university)
Gwak, Ki-Seob (Dept. of Forest Science, College of Agriculture & Life Science, Seoul national university)
Jeung, Eui-Bae (College of Veterinary Medicine, Chungbuk National University)
Chang, Je-Won (Enbita Co. LTD)
Choi, In-Gyu (Dept. of Forest Science, College of Agriculture & Life Science, Seoul national university)
Publication Information
Journal of the Korean Wood Science and Technology / v.33, no.3, 2005 , pp. 72-78 More about this Journal
Abstract
The present study was conducted to evaluate the application of Chamaecyparis obtusa and to investigate potential utilization of essential oil from C. obtusa as plant-based medicine. The antifungal activity of essential oil from leaves and twigs of C. obtusa (Sieb. Et Zucc) was determined and the major components of active fractions against Microsporum canis (KCTC6591) and Trichophyton mentagrophytes (KCTC6077) were identified by GC/MS analysis. In treatment of essential oil from C. obtusa, the strain M. canis was more resistant than the other, T. mentagrophytes. In the agar diffusion assay, essential oil from C. obtusa inhibited hyphal growth of M. canis and T. mentagrophytes at the concentration of more than 5,000 ppm. The zones named B and C in the TLC assay of essential oil from C. obtusa showed antifungal activities. Among four sub-fractions of n-hexane extract from B and C zones, named as B-1, B-2, C-1 and C-2, the C-2 showed the highest antifungal activity. Instrumental GC/MS analysis for sub-fractions showed that a major component of C-1 was ${\alpha}$-terpineol as terpene alcohol, while C-2 contained sesquiterpenes such as elemol, cedrol and eudesmol.
Keywords
Chamaecyparis obtusa; essential oil; antifungal activity; Microsporum canis; Trichophyton mentagrophytes;
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Times Cited By KSCI : 2  (Citation Analysis)
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