• 제목/요약/키워드: cercaria

검색결과 48건 처리시간 0.023초

간흡충 세르카리아의 Chaetotaxy에 관한 연구 (Cercarial Chaetotaxy of Clonoychis sinensis)

  • 민득영;김재진
    • Parasites, Hosts and Diseases
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    • 제26권2호
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    • pp.113-116
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    • 1988
  • 간흡충 세르카리아의 감각유두 분포양상 즉 chaetotaxy를 관찰하였다. 이 세르카리아의 chaetotaxy는 두부(cephalic region)에서 제1열에 5∼6 Ci 1, 4∼5 Ci 2, 5∼6 Ci 3, 제2열에 4 Cii 1, 2 Cii 2, 4 Cii 3, 5∼6 Cii 4, 제3열에 3∼4 Ciii 1, 2∼3 Ciii 2, 및 제 4열에 2 Civ 1, 2∼3 Civ 2, 2∼3 Civ 3의 분포를 보였으며, 몸통 부위 (body region)의 각측(V) 및 때측(D)에서 2 AiV, 1 AiD, 2 AiiV, 1 AiiB,2 kiiiv,2 AiiiD, 1 AivV, 1 AivD, 1 PiiD, 1 PiiiD, 그리고 꼬리 부위(caudal region)에서 2-2-2-2의 분포양상을 보였다. 이 결과를Opisthorchis chabaudi 및 Metagonimus yokugawai에서 보고된 chaetotaxy와 비교하였다.

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Acanthoparyphium tyosenense Yamaguti, 1939의 생활사에 관한 연구 (Studies on the Life History of Acanthoparyphium tyosenense Yamaguti, 1939)

  • 김영길;유지은
    • 한국수산과학회지
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    • 제34권6호
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    • pp.720-728
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    • 2001
  • 1999년 3월부터 2000년 3월까지 서해안 만경강 하구역의 심포리 지선 간석지에서 채집한 갯우렁이 (Lunatia fortuni)와 큰구슬우 렁이 (Neverita didyma)에서 Cercaria yamagutii Ito,1957를 검출하고, 실험실에서 이매패류인 동죽 (Mactra venerifomis)에 감염 시켜 피낭유충이 형성됨을 확인하였고, 동 실험에서 형성된 피낭 유충을 괭이갈매기 (Larus crassiostris)에 경구 감염시켜 10일 후 성충을 얻어 Acanthoparyphium tyosenense Yamaguti, 1939임을 확인하여 지금까지 밝혀지지 않았던 이 충의 전 생활사를 밝혀냈다. 제1중간숙주는 해산 복족류인 갯우렁이, 큰구슬우렁이, Tympanotonus microptera와 Cerithidea (Cerithidea) largillierti, Cerithidea (Cerideopsilloa) cingulata이고, 제 2중간숙주는 이매패인 동죽, 개량조개, 맛조개, 바지락이며, 종숙주는 괭이갈매기와 물오리의 일종인 Melaniua fusca stejnegeri이다. 갯우렁이와 큰구슬우렁에서 검출한 Cercaria yamagutii를 실온 $25^{\circ}C$ 전후에서 동죽이 들어있는 수조에 넣은 후 30분 이내에 입수는 피낭의 크기 $300\sim360{\mu}m$, 탈낭유충$790\sim800\times300\sim310{\mu}m$크기로 활발히 신축 운동하는 성숙한 유충으로 발육되었다. 자연산 이매패에서 Acanthopayphium tyosenense의 피낭유충 기생율은 동죽 $99.5\%$, 맛조개 $76.3\%$, 바지락 $37\%$이었고, 피낭유 충의 부위별 기생율은 동죽의 경우 검출된 피낭유충 총 18,064개중 족부 근육 $62.9\%$ (11,368개), 아가미 $23.6\%$ (4,258개), 내장낭$13.5\%$ (2,435개)의 기생율을 나타냈다. 피낭유충은 숙주 종이나 크기와 관계없이 나타났다.

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간디스토마에 작용하는 천연물 및 그 유사체 (Anthelmintic Natural Products against Clonorchis sinensis and the Analogues)

  • 안병준;이재구
    • 약학회지
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    • 제30권5호
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    • pp.253-265
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    • 1986
  • Among 230 species of herbal drugs screened, thirty one show the anthelmintic activities in vitro against Clonorchis sinensis, the chinese liver fluke. The active substances have been isolated and their structures identified. Some of the active substances and their derivatives have been synthesized, followed by anthelmintic activity tests. The extracts from the active drugs cause damages in organs of the adult worm in the hepatic duct of rabbit. Some fresh water fish, which function as bad hosts for the fluke, excrete defense substances against the cercaria of the fluke. The defense substances have been isolated from Cyprinus carpio and Cyprinus carpio nudus, followed by structural identification. The results are summarized as follows: l) The bark of Machilus thunbergii as well as the seed of Schizandra chinensis contain meso-dihydro-guaiaretic acid as the anthelmintic component. Among derivatives synthesized, 4-phenyl-1-((3, 4-dihydroxyphenyl)-, 4-phenyl-1-(3-hydroxy-4-methoxy phenyl)-and 4-phenyl-1-(4-hydroxy-3-methoxy phenyl)-2, 3-dimethyl butanes show considerable activities. Administration of the bark extract mainly damages the bladder of the adult worm. 2) The active substance from the roasted fruit of Prunus mume is 2-hydroxymethylfurfural. This substance is produced during the roasting process. Administration of the fruitextract causes a damage of the bladder of the adult worm. 3) The active substance from the root of Scutellaria baicalensis is 5.2'-dihydroxy-6, 7, 8, 6'-tetramethoxyflavone. 4) Beside alantolactone, a very strong anthelmintic component is contained in the root of Inula helenium. Administration of the root extract causes irreversible damage on the worm, affecting mainly the reproductive organs. 5) The cercaricidal substances from the epidermis of C. carpio and C. carpio nudus are ethyl linoleate and linoleic acid, respertively. 6) The cercaricidal substances from various kinds of fresh water fish have different $R_f$ values, implying that the defense substances are species-specific. Unexpectedly, the fish with good host function, for example Pseudorasbora parva, excrete the defense substances, too. The defense substances are possibly organ-specific in individual species; the organs essenstial for the existence of the species excrete the defense substances, allowing other parts to be invaded by the cercaria. 7) The cercaricidal fraction of Carassius carassius is detected only in the fish which have been collected during the summer time from May to September, Its secretion is not dependent on water temperature. Thus, it seems to be possible that the secretion of the defense substance would be stimulated through a contact between the fish and cercaria.

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간흡충 제이중간숙주에 관한 실험적 연구 IV. 치리체내에 있어서 간흡충 피낭유충의 운명 (Experimental Studied on the Second Intermediate HoBts of Clonorchis sinensis IV. Observations on the fate of metacercariae of Clonorchis sinensie in the fish hast, CuffricuEus eigenmunni)

  • 이재구;백병걸;이호일
    • Parasites, Hosts and Diseases
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    • 제23권1호
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    • pp.79-86
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    • 1985
  • In order to judge appropriately the suitability as the second intermediate host of Clonorchis sinensis, the present experiments were proposed to survey the metacercaria of C. sinensis in the fish host, Cuztriculus eigenmanni, in the 6eld, and to observe, by experimental infection of the fish with Clonorchis cercaria, the penetrating ability of the cercaria, maturity, the process of degeneration and extinction, and infectivity of the metacercaria. The following is a brie summary of the leading facts gained through the experiments. 1. All the ten fish (mean body weight $13.4{\pm}3.439$) which were caught in Ok-ku irrigation reservoir were in acted with the metacercariae and the average number of the metacercariae found in the fish body/gram was 4.55. 2. The Bish was easily subject to invasion of the cercaria, and the examination of the cercariae after having the fish infected disclosed that 5:l hours after the infection, all of the cercariae formed their cysts in muscle and the metacercariae kept growing, that 7 days later the metacercariae were found folding their bodies twice, and that in 15 days the characteristic structure of the metacercariae was complete and made a vigorous rotary movement intermittently. 3. In the case of control fish, Pseudorasbora parka, both penetrating ability of the cercariae and the infective degree of the metacercariae to control host were stronger as compared with the 5sh host because that the control fish had higher suceptibility to C. sinensis. 4. Afterwards, the metacercariae in the fish host came to a state of maturity and beyond this stage some metacercariae started the Process of degeneration and extinction in as early as 30 days, and as more days elapsed, mortality of the metacercariae was not uniformly increased but difference of mortality appeared to be according to the individual of Ssh host. That is, in 390, 540 and 586 days, all of the metacercariae in the fish hosts became dead, meanwhile almost all of those found in the fish host of 596 days after the infection survived (mortality; 8, 51%) , Percentage of orally administered metacercariae developing to maturity in rabbit is 10.465 in the last case. 5. As the days were progressed the shapes of the metacercariae in the fish host tended to change from ellipsoid to almost round shapes, the length became shorter while the width was wider. The results shown above revealed that C. eigenmanni was, though it could not be proper host than p. larva. suitable as the second intermediate host in addition to our previous observation results.

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해산 이매패에 기생하는 흡충, Acanthoparyphium tyosenense Yamaguti, 1939의 생활사에 관한 연구

  • 유지은;김영길
    • 한국어업기술학회:학술대회논문집
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    • 한국어업기술학회 2000년도 춘계수산관련학회 공동학술대회발표요지집
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    • pp.427-428
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    • 2000
  • 본 연구는 우리 나라 간석지 산 패류에서의 흡충류 분류와 생활사 그리고 패류의 병해를 구명할 목적으로 1999년 3월부터 2000년 3월까지 만경강 하구 역에 위치한 심포 지선 간석지 애서 채집한 갯우렁이(Lunatia fortuni)와 큰구슬우렁이에서 Cercaria yamagutii Ito, 1957 동죽(Mactra veneriformis)과 맛조개 및 바지락에서 Acanthoparyphium tyosen Yamaguti,1939의 피낭유충을 검출하고, 이를 실험실에서 감염실험을 실시한 바, 지금까지 Yamaguti(1939) 와 Ito(1956), 김 등(1984, 1988)이 밝히지 못한 Acanthoparyphium tyosene의 전 생활사가 밝혀졌기에 보고한다. (중략)

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이전고환극구흡충의 실험실 내 생활사 (Experimental life history of Echinostoma cinetorchis)

  • 이순형;채종일
    • Parasites, Hosts and Diseases
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    • 제28권1호
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    • pp.39-44
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    • 1990
  • 실험실 내에서 또아리물달팽이(Hippeutis cantori)를 중간숙주로 한 이전고환극구흡충(E. cinetorchis)의 생활사를 관찰하였다. 실험감염시킨 흰쥐로부터 회수한 충체에서 충란을 얻어 28$^{\circ}C$의 물에서 배양하였던 바, 배양 24일에 탈각 부화하였으며 부화한 미라시디움(miraciaium)은 체표면에 밀생해 있는 섬모를 이용하여 매우 빠른 속도로 유영하였고, 크기는 평균 $102.5{\times}36.8{\mu\textrm{m}}$이었다. 미라시디움을 실험실에서 부화시킨 패류에 접촉 감염시 킨 후 유미유충의 유출_여부와 파쇄법에 의한 유충의 감염 상태를 조사하였다. 피낭유충은 검사한 16마리 패류 모두에서 17~321개가 검출되었고, 레디아(regia)와 유미유충(cercaria)이 3마리의 패류에서 다수가 발견되었다. 레디아는 길쭉한 자루 모양이었으며 평균 $1.43{\times}0.24{\;}mm$의 크기이었고, 근육성의 인두와 길쭉한 맹장 및 성숙한 유미유충을 보유하고 있었다. 유미유충은 타원형의 몸체와 막대 모양의 꼬리로 이루어져 있었고 몸체에는 두개 의 흡반과 인두 및 특징적인 광택성의 배설 과립이 존재하였다. 피낭유충은 원형 또는 타원형이었으며 평균 $139.0{\times}134.4{\mu\textrm{m}}$이었다. 감염 후 17일에 회수한 충체는 크기가 평균 $10.3{\times}2.4{\;}mm$이었으며, 두극의 수 및 배열, 고환의 수 및 위치 등에 있어서 이전고환극구흡충의 특징적인 소견을 나타내었다. 자연 유출된 유미유충은 전혀 관 찰할 수 없었다. 이 연구를 통하여 우리 나라에서 논과 수로에 흔히 서식하는 또아리물달팽이가 이전고환극구흡충의 제 1중간 숙주이고 동시에 제 2중간숙주가 될 수 있음을 실험적으로 확인하였다. 이러한 중간숙주로서의 역할은 자연계에서도 이루어질 것으로 추측된다.

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Metagonimus yokogawai 세르카리아의 감각유두에 관한 연구 (Study on Sensory Papillae of Metagonimus yokogawai Cercaria)

  • 김재진;민득영소진탁
    • Parasites, Hosts and Diseases
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    • 제22권1호
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    • pp.11-20
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    • 1984
  • A number of studies on the papillae of cercariae of trematodes reported that the papillar patterns (or chaetotaxy) of cercariae might be an excellent method to attain better understanding of the digenetic trematodes (Richard, 1971 ; Short and Cartrett, 1973; Bayssade-Dufour, 1979) . The present study was aimed to determine the number, distribution pattern and structure of the sensory papillae of Metagonimus yokogawai cercariae, and to elucidate the chaetotaxy of this digenetic trematode. M. yokogawai cercariae were pipetted from a vial in which infected snails (Semisulcospira libertina) had been kept for 3 hours. The snails were collected from an endemic area of M. yokogawai, Boseong river in west-southern part of Korea. Observations of papillae were based on light microscopy of those stained with silver nitrate, and on scanning electron microscopy The results are summarized as follows: 1, All papillae observed were uniciliated. 2. Cilia in anterior tip were shorter than the others in other portions. 3. The body papillae were arranged in essentially symmetrical patterns, Total number of the papillae was 126(63 pairs) in average; anterior tip 40(20 pairs), ventral 20(10 pairs), lateral 42(21 pairs), and caudal 8(4 pairs). 4. The chaetotany of M. yokogawai cercaria was: Ci cycle ($3+3C_{I}V,{\;}2+2C_{I}L,{\;}2+3C_{I}D),{\;}C_{II}{\;}cycle(2C_{II}V,{\;}1C_{II}L,{\;}2C_{II}D),{\;}C_{lll}{\;}cycle{\;}(1+lC_{III}V,{\;}1C_{IlI}L),{\;}C_{IV}{\;}cycle{\;}(1C_{IV}V,{\;}IC_{lV}L){\;}in{\;}cephalic{\;}region:{\;}A_I(1A_{IV}V,{\;}1+2A_{I}L,{\;}1A_{I}D),{\;}A_{II}(1A_{II}V,{\;}1+3A_{II}L,{\;}1A_{II}D),{\;}A_{III}(1A_{III}V,{\;}1+1A_{III}L,{\;}1A_{III}D){\;}and{\;}A_{IV}(1A_{IV}V,{\;}2A_{IV}L)$ in antacetabular region: $1M_{I}V{\;}and{\;}2M_{I}L$ in median: $1+1P_{I}L,{\;}1P_{II}L,{\;}1P_{II}D,{\;}1P_{III}L,{\;}1P_{IV}L{\;}and{\;}1P_{IV}D$ in postacetabular region: 2-2-2-2 in caudal region.

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Pathology Survey of the Manila Clam Ruditapes philippinarum from Hwangdo Tidal Flat in Cheonsu Bay on the West Coast of Korea

  • Yang, Hyun-Sung;Cho, Young-Ghan;Shin, Jong-Seop;Park, Heung-Sik;Choi, Kwang-Sik
    • Ocean and Polar Research
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    • 제43권4호
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    • pp.365-370
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    • 2021
  • Manila clam Ruditapes philippinarum is present at high rates of density in tidal flats in Cheonsu Bay on the west coast of Korea, where clams often exhibit mass mortalities in late summer. We monitored the pathologic condition of clams at Hwangdo tidal flat (HD) to understand the parasitic impacts on clam fitness. Manila clams were fully ripe in July and spawned during August and September, as the histology indicated. The histology revealed that clams in HD tidal flats were heavily infected by the protozoa parasite Perkinsus olseni, as the monthly prevalence ranged from 53% (September) to 93% (August). In addition, Manila clams were co-infected by the metazoan parasite Cercaria tapetis and Parvatrema duboisi with the prevalence of 0-33% and 0-14%, respectively. Massive hemocyte infiltration and subsequent inflammation were commonly observed from the gills of P. olseni infected clams. Clusters of P. olseni trophozoites and heavy hemocyte infiltration were also observed from the female gonad, suggesting that P. olseni interferes with host gonad maturation. The larval trematode occupied almost the entire host gonad, resulting in gonad castration. In addition, Metacercaria of P. duboisi were observed from the subsurface of the mantle. Ray's fluid thioglycollate medium assay (RFTM) indicated that clams collected in August and September contained approximately 4.0×106 P. olseni cells/g gills. Condition Index (CI) declined gradually from spring to early summer, and the decline in CI was interpreted as a consequence of the heavy parasitism, as the parasites drain the host's net energy to be used in somatic growth and gamete production.

간흡충에 대한 살충성 물질에 관한 연구 IV. 이스라엘잉어(향어) 점액으로부터 살충성 물질 분획 (The Wormicidal Substance of Fresh Water Fishes on Clonorchis IV. Preliminary research on the wormicidal substance from mucus of Cyprinus carpio nudes)

  • 이재구;김평길
    • Parasites, Hosts and Diseases
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    • 제21권1호
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    • pp.21-26
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    • 1983
  • As a series of studios on the suitability as a second intermediate host of Clcnorchis sinensis, the wormicidal effects of mucus from epidermis of Cyprinus carpio nudus were observed. The results obtained were sumlnarized as follows : On the silica gel thin layer chromatography with the greenisll yellow supernatant in acetone: benzene110:90 as carrier, seven spots were observed in iodine chamber. The spot of Rf. 0.225 value among them disclosed the strongest wormicidal effect on the cercaria, the encysted metacercaria, and the adult of C. sinensis. hfeanwhile, no seasonal variations were found in Rf. values and effect of the wormicidal fractions. The wormicidal materials in epidermal mucus of C. carpio nudes and Carassius carassius were compared in their effect, and the former was Inore effective killing substance than the latter. It seemed that C. carpio nodus could not be proper intermediate host of C. sinensis according to results of the present study, in addition to our previous observation results.

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